Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,159,354	C→T	65.0%	R323R (CGC→CGT)	mdtC →	multidrug efflux system, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,159,354	0	C	T	65.0%	92.3 / 86.3	97	R323R (CGC→CGT)	mdtC	multidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (18/16);  new base T (27/36);  total (45/52)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGG  >  NC_000913/2159256‑2159452                                                                                                   |                                                                                                   gCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGc                                                                                                    <  1:2694176/99‑1 (MQ=255)  ccAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGc                                                                                                    <  1:2210283/98‑1 (MQ=255)  ccAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTc                                                                                                  >  2:1876998/1‑100 (MQ=255)     atatTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTcccc                                                                                               <  1:2523011/100‑1 (MQ=255)      tattatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTccccc                                                                                              <  1:1227603/100‑1 (MQ=255)      tattatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTccccc                                                                                              >  1:3322463/1‑100 (MQ=255)         tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCccacc                                                                                           >  1:1204241/1‑100 (MQ=255)         tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCccacc                                                                                           >  1:57538/1‑100 (MQ=255)         tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCccacc                                                                                           <  2:103054/100‑1 (MQ=255)         tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccacc                                                                                           <  1:948624/100‑1 (MQ=255)         tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccacc                                                                                           >  2:2471429/1‑100 (MQ=255)           tCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCAt                                                                                         >  1:2012359/1‑100 (MQ=255)            ccAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCAtt                                                                                        >  2:2302541/1‑100 (MQ=255)             cAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTc                                                                                       >  2:2625172/1‑100 (MQ=255)             cAGACGGTTGACAGCATCCGGGC‑AAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTcg                                                                                      >  1:131091/1‑100 (MQ=255)               gACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCCGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTcgc                                                                                     <  1:2533242/100‑1 (MQ=255)                aCGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTcgcg                                                                                    <  2:1272863/100‑1 (MQ=255)                 cGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCgcgc                                                                                   <  1:1287743/100‑1 (MQ=255)                   gTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCt                                                                                 >  1:1558223/1‑100 (MQ=255)                   gTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGcc                                                                                  <  1:903726/99‑1 (MQ=255)                    ttGACAGCATCCGGGCAAAATTACCTGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTc                                                                                <  1:3086105/100‑1 (MQ=255)                      gACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGc                                                                              <  2:115648/100‑1 (MQ=255)                       aCAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCt                                                                             >  2:3118050/1‑100 (MQ=255)                        cAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTg                                                                            >  1:1307040/1‑100 (MQ=255)                        cAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTg                                                                            <  2:3113743/100‑1 (MQ=255)                        cAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTg                                                                            <  1:2651696/100‑1 (MQ=255)                          gCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTgg                                                                           >  2:327977/1‑99 (MQ=255)                          gCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGga                                                                          <  2:1651520/100‑1 (MQ=255)                           cATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaa                                                                         <  2:175787/100‑1 (MQ=255)                            aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaag                                                                        <  2:3132786/100‑1 (MQ=255)                                 ggCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGt                                                                    <  1:1955109/99‑1 (MQ=255)                                  gCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCg                                                                  <  1:2610404/100‑1 (MQ=255)                                  gCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCg                                                                  >  1:680865/1‑100 (MQ=255)                                   cAAAATTACCGTAGTTGCAGGAAATCATGCCGGCCGCCACTGATCTGCAGACTGCCCAGAGCCGCTCGCCCCCCACTCGCGCCTCGCTGGAAGAAGTc                                                                   <  1:326321/98‑1 (MQ=255)                                   cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGa                                                                 <  1:708815/100‑1 (MQ=255)                                   cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGa                                                                 >  1:397330/1‑100 (MQ=255)                                     aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc                                                               <  2:1316136/100‑1 (MQ=255)                                      aaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCa                                                              <  2:3374716/100‑1 (MQ=255)                                       aTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTAGAGCaa                                                             >  1:1507278/1‑100 (MQ=255)                                           ccGGTGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGc                                                         <  1:895485/100‑1 (MQ=255)                                             ggAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTg                                                       <  1:86220/100‑1 (MQ=255)                                              gAGTTGCCGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGa                                                      <  1:1540921/100‑1 (MQ=255)                                                tctGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAtt                                                    <  2:2265869/98‑1 (MQ=255)                                                gTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAtt                                                    >  2:2236146/1‑100 (MQ=255)                                                gTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAtt                                                    >  2:2915790/1‑100 (MQ=255)                                                  tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                                  >  2:1468235/1‑100 (MQ=255)                                                  tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                                  >  2:1028/1‑100 (MQ=255)                                                  tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                                  >  1:1538616/1‑100 (MQ=255)                                                  tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                                  <  1:1817025/100‑1 (MQ=255)                                                   gCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTaga                                                 >  1:2265853/1‑98 (MQ=255)                                                    cAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtct                                                >  1:340695/1‑100 (MQ=255)                                                     aGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtctc                                               <  1:2595035/100‑1 (MQ=255)                                                      ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                              >  2:179647/1‑100 (MQ=255)                                                      ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                              >  2:1226826/1‑100 (MQ=255)                                                      ggAAACCATTCCGGCGGCGATTGACCTGCAACTTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGCAGAAGTCGCGCAAACGCTGATTACCTCg                                              >  1:400000/1‑100 (MQ=255)                                                       gAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCgg                                             <  2:790586/100‑1 (MQ=255)                                                        aaaCCATTCCGGCGGCTACTGATCTGCAAATTGCGAAGGGTCGTTCGCCCACCATTCGCGCCTCGCTGGAAGAGGTCGGGCAAACGCTGATGATCTCgg                                             <  1:1008226/99‑1 (MQ=255)                                                           ccATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgc                                         >  2:2682747/1‑100 (MQ=255)                                                            cATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTgg                                          >  1:1571997/1‑98 (MQ=255)                                                            cATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgc                                         >  1:3243701/1‑99 (MQ=255)                                                             aTTCGGGCGGCGATTGATCTGCACATTGCCGAGGATCGTGCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgcg                                        <  1:1272754/99‑1 (MQ=255)                                                               tCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTg                                     >  2:3294253/1‑100 (MQ=255)                                                                ccGGCGGCTATTGATCTGCAAATTGCCCAGGATCGTATCCCCACCTTTCCCGCCGCGGTGGACGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTgg                                    <  1:1308282/100‑1 (MQ=255)                                                                ccGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTgg                                    >  1:207502/1‑100 (MQ=255)                                                                ccGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTgg                                    >  2:1292042/1‑100 (MQ=255)                                                                 cggcggCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt                                   <  2:332974/100‑1 (MQ=255)                                                                 cggcggCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt                                   <  2:207502/100‑1 (MQ=255)                                                                    cggcgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGAt                                <  1:1825735/100‑1 (MQ=255)                                                                    ccgcgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCCCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGAt                                <  1:1292038/98‑1 (MQ=255)                                                                        gATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATGCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtg                            <  2:2689379/100‑1 (MQ=255)                                                                         aTTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGGAGAAGTCGAGCAAACACTGATTATATCGGTGGCGCTGGTGTTTCt                             >  1:2287994/1‑98 (MQ=255)                                                                         aTTGATCTGCAAATTGACCAGGACCGTTCCACCTTTATTCCTTTCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtgg                           <  1:829054/100‑1 (MQ=255)                                                                          ttGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggt                          <  2:2005067/100‑1 (MQ=255)                                                                           tgctCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtg                         <  1:1836497/97‑1 (MQ=255)                                                                           tGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtg                         <  2:2430148/100‑1 (MQ=255)                                                                            gATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtgg                        <  2:95530/100‑1 (MQ=255)                                                                            gATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtgg                        <  2:200636/100‑1 (MQ=255)                                                                              tcgggaagttGCCGAGGATCGTTCCCCCGCCATTGGCGCTGCGGTGGGAGAAGTCGAGCAACCGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg                      <  1:3340823/92‑1 (MQ=255)                                                                                tGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGGTGGTGATTCt                             >  1:2852015/1‑91 (MQ=255)                                                                                tGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt                    >  2:568264/1‑100 (MQ=255)                                                                                tGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt                    >  2:380540/1‑100 (MQ=255)                                                                                tGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCGCGGTGAttttaaag                         >  1:250813/1‑89 (MQ=255)                                                                                tGCAAATTGCCCAGGATCGCTCCCCCGCCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt                    >  2:2059542/1‑100 (MQ=255)                                                                                 gCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttt                   >  2:1359714/1‑100 (MQ=255)                                                                                  cAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtttt                  <  2:1036652/100‑1 (MQ=255)                                                                                  cAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtttt                  <  2:3145374/100‑1 (MQ=255)                                                                                    aaTTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTa                <  2:946125/100‑1 (MQ=255)                                                                                     aTTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTAt               <  2:2012372/100‑1 (MQ=255)                                                                                       tGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGCTTTTATTc             >  1:941631/1‑100 (MQ=255)                                                                                        gCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTcc            >  2:2101726/1‑100 (MQ=255)                                                                                        gCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTcc            <  2:1275643/100‑1 (MQ=255)                                                                                        gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTcc            <  2:3097675/100‑1 (MQ=255)                                                                                         cccAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGACTCTGGTGGTGAATTTATTGCt           >  1:1379192/1‑100 (MQ=255)                                                                                          ccAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg          <  2:568620/100‑1 (MQ=255)                                                                                          ccACGAACGCGCCGCCAACCTTCGCGCCTGGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg          <  1:1577298/100‑1 (MQ=255)                                                                                           cAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTgc         <  2:2588892/100‑1 (MQ=255)                                                                                           cAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTCGTGACTCTGGTGGTGTTTTTATTCCTgc         >  1:230700/1‑100 (MQ=255)                                                                                             ggATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTgcgc       >  2:3281131/1‑100 (MQ=255)                                                                                                  gCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTCGTGATTCTGGTGGAGTTTTTATTCCTGCGCTCggg  >  1:1809265/1‑100 (MQ=255)                                                                                                   |                                                                                                   GCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGG  >  NC_000913/2159256‑2159452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑