Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,161,946 A→G 58.8% A161A (GCA→GCG iceT → putative citrate/iron‑citrate/zinc‑citrate efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,161,9460AG58.8% 83.5 / 117.9 116A161A (GCA→GCGiceTputative citrate/iron‑citrate/zinc‑citrate efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (24/24);  new base G (38/30);  total (62/54)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GCGCGAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCGGCGC  >  NC_000913/2161847‑2162045
                                                                                                   |                                                                                                   
 cgcgAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCAt                                                                                                    >  1:1071356/1‑100 (MQ=255)
    gAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGcgtcgt                                                                                                 <  1:1066422/100‑1 (MQ=255)
     aGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTg                                                                                                >  1:3334166/1‑100 (MQ=255)
       cAATGTATGGCGGCGATGACCTTTGGCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGc                                                                                              <  1:1109204/100‑1 (MQ=255)
       cAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGc                                                                                              >  1:81935/1‑100 (MQ=255)
        aaTATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCa                                                                                             <  1:952826/100‑1 (MQ=255)
          tatatGGCGGCGATGACCTTTGTCACGTTACCCGATCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACt                                                                                           <  2:2904874/100‑1 (MQ=255)
             atGGCGGCGATGACCTTTGTCACGTTACCCGGTCTGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTCCGCATCGTGGCACTGGa                                                                                        <  1:2314101/100‑1 (MQ=255)
               ggcggcGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATc                                                                                      <  2:2422568/100‑1 (MQ=255)
               ggcggcGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATc                                                                                      >  2:3235792/1‑100 (MQ=255)
                gcggctATGACCGGTGTGACGGTACCGGGTCAGGTCGGTCCGCTGCGCGGTCCGGCGCACAGCGGTCTGCTGGTGGATTACGCATCGTGGCACTGGATc                                                                                      <  1:1578256/99‑1 (MQ=255)
                gcggcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGATCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCt                                                                                     >  1:656931/1‑100 (MQ=255)
                 cggcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGTTCtt                                                                                    >  2:250318/1‑100 (MQ=255)
                   gcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCtttt                                                                                  >  1:1522954/1‑100 (MQ=255)
                      aTGACCATTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCttttt                                                                                 <  1:2853627/98‑1 (MQ=255)
                       tGACCTTTGTCACGTGACCCGGTCAGGTCGGTCGGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGAt                                                                              <  1:130233/100‑1 (MQ=255)
                        gACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATc                                                                             >  1:2215992/1‑100 (MQ=255)
                         aCCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCa                                                                            >  2:476418/1‑100 (MQ=255)
                         aCCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCa                                                                            >  1:1167033/1‑100 (MQ=255)
                          ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCaa                                                                           >  1:215957/1‑100 (MQ=255)
                          ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCaa                                                                           >  2:540803/1‑100 (MQ=255)
                             ttGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACAt                                                                        >  1:1064833/1‑100 (MQ=255)
                                tCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTcc                                                                     >  2:16312/1‑100 (MQ=255)
                                tCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTcc                                                                     >  2:1886697/1‑100 (MQ=255)
                                 naCGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCg                                                                    >  1:3075329/2‑100 (MQ=255)
                                  aCGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCgg                                                                   <  2:411982/100‑1 (MQ=255)
                                  aCGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCgg                                                                   <  2:2574600/100‑1 (MQ=255)
                                   cGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGt                                                                  >  2:2993565/1‑100 (MQ=255)
                                   cGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGt                                                                  <  1:576499/100‑1 (MQ=255)
                                    gTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGTTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTg                                                                 >  2:811315/1‑100 (MQ=255)
                                     ttACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTgg                                                                <  2:3231618/100‑1 (MQ=255)
                                      tACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTggg                                                               >  2:2157505/1‑100 (MQ=255)
                                      tACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTggg                                                               <  2:1095788/100‑1 (MQ=255)
                                      tACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTggg                                                               <  2:1844282/100‑1 (MQ=255)
                                       aCCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTgggg                                                              >  1:3377526/1‑100 (MQ=255)
                                          cGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGaat                                                           >  2:1749610/1‑98 (MQ=255)
                                          cGGTCAGGTCGGTCCGCTGCTCGCTCCGGCGGTCGGCGGTCTGCTGGTGGAGTACGCGCCGTGGCACGGGCCCTGTTTGAACAACATTCCGGtcg                                                                >  1:1538661/1‑93 (MQ=255)
                                             tCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATc                                                        <  1:1800555/100‑1 (MQ=255)
                                             tCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATc                                                        >  1:283476/1‑100 (MQ=255)
                                              cAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCg                                                       >  2:1795092/1‑100 (MQ=255)
                                               aGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCgg                                                      >  2:3158579/1‑100 (MQ=255)
                                                ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGt                                                     >  2:1292750/1‑100 (MQ=255)
                                                ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGt                                                     >  2:1804003/1‑100 (MQ=255)
                                                 gtcggtgCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                    <  1:3035463/100‑1 (MQ=255)
                                                 gtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                    <  1:1965448/100‑1 (MQ=255)
                                                  tcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGc                                                   <  2:2139960/100‑1 (MQ=255)
                                                  tcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGGCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                    <  1:161710/99‑1 (MQ=255)
                                                   cggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCg                                                  <  2:1284338/100‑1 (MQ=255)
                                                    ggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGa                                                 >  2:1317987/1‑100 (MQ=255)
                                                      tcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATc                                               <  1:776805/100‑1 (MQ=255)
                                                       gatctgctCGGTCTGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCg                                              <  1:816140/97‑1 (MQ=255)
                                                        cGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGc                                             >  2:519040/1‑100 (MQ=255)
                                                         gctgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGcc                                            <  1:2617801/100‑1 (MQ=255)
                                                         gctgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGcc                                            <  2:1340779/100‑1 (MQ=255)
                                                         gctgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGcc                                            <  2:3268364/100‑1 (MQ=255)
                                                          ctgcgCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGcc                                            <  1:704841/99‑1 (MQ=255)
                                                           tgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCcac                                          >  2:2022369/1‑100 (MQ=255)
                                                            gctCGGTCTGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCgagatc                                         >  2:816143/1‑95 (MQ=255)
                                                              tCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACAtt                                       >  1:2121362/1‑100 (MQ=255)
                                                               cGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTg                                      >  2:1075787/1‑100 (MQ=255)
                                                               cGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTg                                      >  2:2429187/1‑100 (MQ=255)
                                                                ggTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGc                                     <  2:1770812/100‑1 (MQ=255)
                                                                 gTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCt                                    >  2:2595858/1‑100 (MQ=255)
                                                                 gTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCt                                    >  2:2338064/1‑100 (MQ=255)
                                                                  tCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTg                                   <  2:2625182/100‑1 (MQ=255)
                                                                  tCCGGCGCTCGGCGGTCTGCTGGTGGAGGACGCGTCGTGGCACTGGAGCTTTTTGCTCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTg                                   <  1:3010633/100‑1 (MQ=255)
                                                                    cGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGtt                                 <  2:1148150/100‑1 (MQ=255)
                                                                        gcTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATg                             <  2:1277251/100‑1 (MQ=255)
                                                                           cggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCg                          >  2:2697564/1‑100 (MQ=255)
                                                                           cggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCg                          <  2:2436174/100‑1 (MQ=255)
                                                                           cggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGAACTTTTTGATCAACATTCCGGTGGGGATTATCGCTGCGATCGCCACATTGCTGTTAATGcc                           >  1:1301284/1‑99 (MQ=255)
                                                                              cggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                       >  2:512372/1‑100 (MQ=255)
                                                                              cggTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTAATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                       <  1:3252963/100‑1 (MQ=255)
                                                                               ggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACt                      >  1:1885182/1‑100 (MQ=255)
                                                                               ggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACt                      <  2:1167037/100‑1 (MQ=255)
                                                                                gTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTa                     <  1:2993539/100‑1 (MQ=255)
                                                                                gTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTa                     >  2:1572948/1‑100 (MQ=255)
                                                                                 tatgctgGTGGAGTAGGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTac                    <  1:2825248/98‑1 (MQ=255)
                                                                                  ctgctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTaca                   <  2:3057915/100‑1 (MQ=255)
                                                                                   tgctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTacac                  >  2:1860239/1‑100 (MQ=255)
                                                                                   tgctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTacac                  <  2:3156401/100‑1 (MQ=255)
                                                                                    gctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACAcc                 >  1:3335957/1‑100 (MQ=255)
                                                                                    gctgGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACAcc                 <  2:3377555/100‑1 (MQ=255)
                                                                                     ctgGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCa                <  2:1697738/100‑1 (MQ=255)
                                                                                      tggtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCTACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCAt               >  1:2079908/1‑100 (MQ=255)
                                                                                      tggtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCAt               >  2:3259925/1‑100 (MQ=255)
                                                                                       ggtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATg              >  1:225431/1‑100 (MQ=255)
                                                                                        gtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCTAACTACACCATGc             <  2:2276479/100‑1 (MQ=255)
                                                                                         tggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCa            >  2:434391/1‑100 (MQ=255)
                                                                                          ggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAg           >  2:2090482/1‑100 (MQ=255)
                                                                                           gAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGa          <  1:2628630/100‑1 (MQ=255)
                                                                                            aGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAc         >  1:2027093/1‑100 (MQ=255)
                                                                                            aGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAc         >  1:1503478/1‑100 (MQ=255)
                                                                                               aCGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAcgcg      <  2:518324/100‑1 (MQ=255)
                                                                                                cgcgTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACgcgg     >  1:2406693/1‑100 (MQ=255)
                                                                                                cGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACCCCATGCAGACgcgg     >  2:400225/1‑100 (MQ=255)
                                                                                                 gCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACgcggc    <  2:1186660/100‑1 (MQ=255)
                                                                                                  cATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACgcggcg   >  1:2382558/1‑100 (MQ=255)
                                                                                                  cATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACgcggcg   >  2:2210197/1‑100 (MQ=255)
                                                                                                   aTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCGgcgc  >  1:231966/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GCGCGAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCGGCGC  >  NC_000913/2161847‑2162045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: