Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,076 C→A 71.3% pseudogene (287/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,0760CA71.3% 199.0 / 101.2 126pseudogene (287/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (21/16);  new base A (46/43);  total (67/59)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 127 total aligned reads displayed.

ATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACA  >  NC_000913/2172979‑2173174
                                                                                                 |                                                                                                  
 tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGcc                                                                                                 >  2:284856/1‑100 (MQ=255)
 tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGcc                                                                                                 <  2:1587583/100‑1 (MQ=255)
 tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGcc                                                                                                 >  2:168076/1‑100 (MQ=255)
 tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGcc                                                                                                 >  2:3327028/1‑100 (MQ=255)
    aaaTACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTg                                                                                              <  2:887257/100‑1 (MQ=255)
       tACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATc                                                                                           <  2:2083700/100‑1 (MQ=255)
        aCCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATcc                                                                                          <  1:1040180/100‑1 (MQ=255)
         ccGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGACAAGCCCTGCTCCa                                                                                         >  2:2446247/1‑100 (MQ=255)
         ccGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCa                                                                                         >  1:1481051/1‑100 (MQ=255)
         ccGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATc                                                                                           >  1:2494861/1‑98 (MQ=255)
         ccGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCa                                                                                         >  2:2369290/1‑100 (MQ=255)
           ggTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAtt                                                                                       <  1:2924982/100‑1 (MQ=255)
           ggTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAtt                                                                                       >  1:2045993/1‑100 (MQ=255)
           ggTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAtt                                                                                       <  2:3248824/100‑1 (MQ=255)
           ggTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAtt                                                                                       >  1:642899/1‑100 (MQ=255)
           ggTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAtt                                                                                       <  2:1362692/100‑1 (MQ=255)
              cAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGaa                                                                                    >  1:2068990/1‑100 (MQ=255)
               aGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAg                                                                                   <  2:3265878/100‑1 (MQ=255)
               aGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAg                                                                                   <  1:361140/100‑1 (MQ=255)
               aGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAg                                                                                   <  2:420058/100‑1 (MQ=255)
                gATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGc                                                                                  <  2:1209815/100‑1 (MQ=255)
                 aTAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGcc                                                                                 >  1:3372836/1‑100 (MQ=255)
                 aTAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGcc                                                                                 >  1:2571145/1‑100 (MQ=255)
                    aaTTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGccacc                                                                              <  1:613537/100‑1 (MQ=255)
                    aaTTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGccacc                                                                              <  2:2642017/100‑1 (MQ=255)
                      ttGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCAt                                                                            <  2:677500/100‑1 (MQ=255)
                       tGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATa                                                                           >  2:668970/1‑100 (MQ=255)
                        gCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATAc                                                                          <  2:2263505/100‑1 (MQ=255)
                          aCCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATAcc                                                                         >  2:2826764/1‑99 (MQ=255)
                            cGATAATAATGCAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCACTGATCCATTGAAGCCACCATAccccc                                                                      >  1:323079/1‑100 (MQ=255)
                             gATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGGAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATAccc                                                                        >  1:249481/1‑97 (MQ=255)
                               taataaTGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGc                                                                   <  1:3057453/100‑1 (MQ=255)
                               taataaTGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGc                                                                   <  2:2157517/100‑1 (MQ=255)
                                 ataatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCtt                                                                 >  1:3299855/1‑100 (MQ=255)
                                  taatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTCATGGGAGAAACACCCTGATCCATTGAAGCCACCATACACCCGGCtat                                                                >  1:1667823/1‑98 (MQ=255)
                                   aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCtttt                                                               <  2:2586993/100‑1 (MQ=255)
                                    attaaaCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTGACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCGCCATTCCCCCGGCTTTTa                                                              <  1:707740/97‑1 (MQ=255)
                                    atGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTa                                                              >  1:912899/1‑100 (MQ=255)
                                     tGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTaa                                                             <  1:1780428/100‑1 (MQ=255)
                                      gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAAc                                                            >  1:1142626/1‑100 (MQ=255)
                                       aaaCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACg                                                           >  2:2650948/1‑100 (MQ=255)
                                          ccGGGAATATTTTGCGGGGAGAAAACATGAATAAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGccc                                                        >  1:2717764/1‑100 (MQ=255)
                                           cGGGACTATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGcccc                                                       <  1:2557803/100‑1 (MQ=255)
                                           cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGcccc                                                       >  2:2981486/1‑100 (MQ=255)
                                             ggAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCa                                                      >  1:1188657/1‑99 (MQ=255)
                                             ggAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAg                                                     <  2:775204/100‑1 (MQ=255)
                                             ggAATATGTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACACCGGGCTTTTAACGCCCAAg                                                     >  1:107940/1‑100 (MQ=255)
                                              gAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGc                                                    <  1:284855/100‑1 (MQ=255)
                                              gAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGc                                                    <  2:639993/100‑1 (MQ=255)
                                                atatTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCan                                                  <  1:1593347/100‑2 (MQ=255)
                                                 tatTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCAtt                                                 >  2:2983426/1‑100 (MQ=255)
                                                   ttttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAg                                               <  2:719204/100‑1 (MQ=255)
                                                     ttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgcc                                             >  2:1324032/1‑100 (MQ=255)
                                                     ttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgcc                                             <  1:1682847/100‑1 (MQ=255)
                                                       gCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgccgc                                           >  1:3143260/1‑100 (MQ=255)
                                                       gCGGGGAGAAAACCTGAATCAGTAACCAGGTAATAGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgccgc                                           >  1:1551691/1‑100 (MQ=255)
                                                        cGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCTTGATCCATTGAAGCCACCATACACACGGCTTTTAACGCCCGAGCATTAgccgcc                                          >  1:868827/1‑100 (MQ=255)
                                                        cGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgcc                                             >  1:246402/1‑97 (MQ=255)
                                                         ggggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCa                                         >  2:106987/1‑100 (MQ=255)
                                                         ggggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCa                                         >  2:106273/1‑100 (MQ=255)
                                                              gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTgg                                    >  2:777870/1‑100 (MQ=255)
                                                               aaaaACTGAATCAGTAACCAGGTAAAGGGAGAAACGCCCTGATCCATTGAAGCCACCATCCACCCGGCTTTTAACGACCCAGCATTAGCCGCCAGTTggg                                   <  1:678901/100‑1 (MQ=255)
                                                                aaaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCTCAGCATTAGCCGCCAGTTggg                                   >  2:1133403/1‑99 (MQ=255)
                                                                aaaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGgt                                  >  2:2997249/1‑100 (MQ=255)
                                                                 aaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGgtg                                 <  1:2085047/100‑1 (MQ=255)
                                                                   ccTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGtgtg                               >  2:2125304/1‑100 (MQ=255)
                                                                   ccTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGtgtg                               <  2:2265443/100‑1 (MQ=255)
                                                                    cTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGc                              <  1:3104/100‑1 (MQ=255)
                                                                    cTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGc                              <  2:3372864/100‑1 (MQ=255)
                                                                    cTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGc                              >  2:1390100/1‑100 (MQ=255)
                                                                    cTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGc                              <  1:3039676/100‑1 (MQ=255)
                                                                     tGAATCAGTAACCAGCTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCa                             <  1:2979272/100‑1 (MQ=255)
                                                                      gAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCaa                            >  2:1720876/1‑100 (MQ=255)
                                                                       aaTCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCaaa                           <  1:2852654/100‑1 (MQ=255)
                                                                          cAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAACCAACCATACACCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCa                        >  1:564657/1‑100 (MQ=255)
                                                                           aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaa                       >  1:328706/1‑100 (MQ=255)
                                                                           aGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaa                       >  2:105290/1‑100 (MQ=255)
                                                                            gTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAAt                      >  2:2095580/1‑100 (MQ=255)
                                                                              aaCCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAg                    <  2:2909320/100‑1 (MQ=255)
                                                                               agcAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCAGACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGt                   >  2:2291390/3‑100 (MQ=255)
                                                                                ccAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAg                    >  1:2166920/1‑98 (MQ=255)
                                                                                 cAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGttt                 >  2:560121/1‑100 (MQ=255)
                                                                                 cAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGttt                 >  2:1665093/1‑100 (MQ=255)
                                                                                  aGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTg                >  1:503804/1‑100 (MQ=255)
                                                                                  aGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATCAGCCGCCAGCTGGGTGTGCAAACCACTAGttt                 >  1:2722082/1‑99 (MQ=255)
                                                                                   ggTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGc               >  2:2702876/1‑100 (MQ=255)
                                                                                    gTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGTCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCg              <  1:122060/100‑1 (MQ=255)
                                                                                    gTAATGGGAGAACCGCCCTGATCCATCGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCg              >  1:2003157/1‑100 (MQ=255)
                                                                                     tAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGt             >  2:2294550/1‑100 (MQ=255)
                                                                                      aaTGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTc            >  2:2809981/1‑100 (MQ=255)
                                                                                       aTGGGAGAACCGCTCTGATCCATTGAAGCCACCATACCTCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcacac           <  1:1279878/100‑5 (MQ=255)
                                                                                        tGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCACTAGCCGCCAGTTCGCCGTACACACCAATAGTTTGCGTcgc          >  1:1392009/1‑100 (MQ=255)
                                                                                         gggAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgcg         >  2:233511/1‑100 (MQ=255)
                                                                                         gggAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgcg         <  2:1970198/100‑1 (MQ=255)
                                                                                          ggAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGa        >  2:2431014/1‑100 (MQ=255)
                                                                                          ggAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGa        >  2:464679/1‑100 (MQ=255)
                                                                                          ggAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGCTGGGTGTGCAAACCAATAGTTTGCGTcgcg         >  1:94476/1‑99 (MQ=255)
                                                                                             gaACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc     <  2:1814424/100‑1 (MQ=255)
                                                                                             gaACAGCCCTCATCCATTGAAACCACCATACCCCCGGCTTTTAAAGCCCCAGCATTAGCCGCCAGTTGAGTGTGCAAACCAATAGTTTGCGTCGCGATcc     <  1:1171426/100‑1 (MQ=255)
                                                                                                ccGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCcaca  <  2:2003170/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
ATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACA  >  NC_000913/2172979‑2173174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: