Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,191,432 C→T 100% G72S (GGC→AGC)  yehC ← putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,191,4320CT98.0% 482.4 / ‑1.8 144G72S (GGC→AGC) yehCputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (68/72);  total (70/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

TTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGC  >  NC_000913/2191336‑2191526
                                                                                                |                                                                                              
tttttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGCACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatc                                                                                             >  2:837214/1‑100 (MQ=255)
tttttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatc                                                                                             <  2:923968/100‑1 (MQ=255)
tttttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatc                                                                                             <  1:2947754/100‑1 (MQ=255)
tttttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatc                                                                                             >  2:1697655/1‑100 (MQ=255)
tttttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatc                                                                                             <  2:806828/100‑1 (MQ=255)
 ttttAGTTGCTGCCTGGAATTTGCCCCTTTTTTTGCCGCTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatca                                                                                            <  1:2600379/100‑1 (MQ=255)
 ttttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatca                                                                                            >  2:122116/1‑100 (MQ=255)
    tACTTGCTGCCCGGAATTTGACCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTAtcatca                                                                                         >  2:3187629/1‑100 (MQ=255)
       ttGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGCATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATc                                                                                      <  1:634730/100‑1 (MQ=255)
            gcCCGGAATTTGCCCCTATTTTTTTTTTTTTTTTCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                 >  1:2988986/1‑100 (MQ=255)
            gcCCGGAATTTGCCCCTATTTTTTTTTTTTTTTGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGTTAATGACGTATCGGCATCATCAATCCACGc                                                                                 >  1:1977321/1‑100 (MQ=255)
            gcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACg                                                                                  >  1:2192701/1‑99 (MQ=255)
            gcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                 >  2:3019827/1‑100 (MQ=255)
            gcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                 <  1:2336505/100‑1 (MQ=255)
            gcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                 >  2:1703801/1‑100 (MQ=255)
            gcCCGGAATTTGCCCCTATTCTTGCCAGTGGTGGCGTGAACATGAAAGGATTCTGAATTTTTTCTGGTGGTAATGACGTATCGTTATCATCAATcc                                                                                     >  1:1975067/1‑96 (MQ=255)
             cccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGcc                                                                                >  1:2956065/1‑100 (MQ=255)
              ccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCt                                                                               >  2:609635/1‑100 (MQ=255)
              ccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCt                                                                               >  2:1752171/1‑100 (MQ=255)
              ccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCt                                                                               >  2:1955645/1‑100 (MQ=255)
                ggAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTgc                                                                             <  2:1667684/100‑1 (MQ=255)
                 gAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTgca                                                                            >  2:2565260/1‑100 (MQ=255)
                  aaTTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTgctg                                                                           <  2:2293307/100‑3 (MQ=255)
                      tGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCagcagc                                                                       <  2:2883999/100‑1 (MQ=255)
                       gCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCg                                                                      <  2:1783854/100‑1 (MQ=255)
                           cTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCgaag                                                                   <  1:311329/99‑1 (MQ=255)
                              tttttGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACg                                                               <  1:1404030/100‑1 (MQ=255)
                               ttttGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACgg                                                              <  2:850387/100‑1 (MQ=255)
                                   gCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTc                                                          >  2:1663538/1‑100 (MQ=255)
                                    ccACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc                                                         <  2:1525930/100‑1 (MQ=255)
                                    ccACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc                                                         >  2:1243692/1‑100 (MQ=255)
                                    ccACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc                                                         >  1:110377/1‑100 (MQ=255)
                                    ccACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc                                                         >  1:2308459/1‑100 (MQ=255)
                                     cACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTccc                                                        <  1:699382/100‑1 (MQ=255)
                                      aCTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCt                                                       <  1:1461691/100‑1 (MQ=255)
                                       cTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCCATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTg                                                      <  1:762869/100‑1 (MQ=255)
                                       cTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGAAGCAGCGAAGAACGGTTTCCCTg                                                      <  1:65909/100‑1 (MQ=255)
                                           tggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtc                                                  <  2:2956091/100‑1 (MQ=255)
                                           tggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtc                                                  <  2:1753754/100‑1 (MQ=255)
                                           tggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtc                                                  <  2:1048814/100‑1 (MQ=255)
                                           tggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtc                                                  >  1:1224141/1‑100 (MQ=255)
                                            ggggTTAACATAAAAGGAAACTGAATTTTTTCTGCTGGTAATGACGTAACGCTATCTTTAATTCACGCCTGCAGTAGCGAAGATCGGTGTCCCTGGt                                                    <  1:2088119/94‑1 (MQ=255)
                                            ggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtca                                                 >  1:1464260/1‑100 (MQ=255)
                                            ggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtca                                                 >  2:830354/1‑100 (MQ=255)
                                              cGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatc                                               >  2:2828714/1‑100 (MQ=255)
                                              cGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatc                                               >  2:1897882/1‑100 (MQ=255)
                                              agTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatc                                               <  1:2755267/99‑1 (MQ=255)
                                               gTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatca                                              <  2:2315227/100‑1 (MQ=255)
                                                 tAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                            >  1:1371163/1‑100 (MQ=255)
                                                  aaCATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACt                                           <  2:1977828/100‑1 (MQ=255)
                                                   aCATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCACTCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTg                                          <  1:1673225/100‑1 (MQ=255)
                                                    cATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCTCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGc                                         <  2:3006295/100‑1 (MQ=255)
                                                    cATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGc                                         >  2:823492/1‑100 (MQ=255)
                                                    cATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGc                                         >  2:1943539/1‑100 (MQ=255)
                                                    cATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGc                                         >  1:3352808/1‑100 (MQ=255)
                                                    cATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGc                                         <  2:3193409/100‑1 (MQ=255)
                                                     aTGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCa                                        >  2:2730982/1‑100 (MQ=255)
                                                       gAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCCTCAACTGCAcc                                      >  2:851765/1‑100 (MQ=255)
                                                       gAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAcc                                      <  2:2179763/100‑1 (MQ=255)
                                                         aaGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCAt                                    <  2:854564/100‑1 (MQ=255)
                                                           ggAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCa                                  <  2:2656565/100‑1 (MQ=255)
                                                             aaCCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCAct                                >  2:3265813/1‑100 (MQ=255)
                                                             aaCCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCAct                                <  2:1464266/100‑1 (MQ=255)
                                                             aaCCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCAc                                 <  1:1536017/99‑1 (MQ=255)
                                                              aCCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActt                               <  2:2121972/100‑1 (MQ=255)
                                                               ccTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtc                              >  2:2265652/1‑100 (MQ=255)
                                                               ccTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtc                              <  2:2842661/100‑1 (MQ=255)
                                                                cTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtct                             >  1:2526453/1‑100 (MQ=255)
                                                                cTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtct                             >  2:719551/1‑100 (MQ=255)
                                                                cTGAATATAATCTGGAGGTCATGACGTATCGCTATCATCAATCCACGTCTGCAGCAGCGAAGAACGGGTTCCCTGGTTCATCAACTGCACCATCActtc                              >  1:118143/1‑99 (MQ=255)
                                                                 tGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtctt                            <  1:3185529/100‑1 (MQ=255)
                                                                    aTTTTTTCTGGTGGTCATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt                         <  1:3078714/100‑1 (MQ=255)
                                                                    aTTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt                         <  1:837211/100‑1 (MQ=255)
                                                                    aTTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt                         >  1:1910139/1‑100 (MQ=255)
                                                                          tCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTg                   <  1:2501682/100‑1 (MQ=255)
                                                                           cTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGc                  <  1:1017836/100‑1 (MQ=255)
                                                                            tggtggTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc                 >  2:391471/1‑100 (MQ=255)
                                                                             ggtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCg                >  2:2611525/1‑100 (MQ=255)
                                                                             ggtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCg                >  2:1004645/1‑100 (MQ=255)
                                                                             ggtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCg                <  2:432642/100‑1 (MQ=255)
                                                                             ggtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCg                >  2:1521897/1‑100 (MQ=255)
                                                                             cttccgatctaCGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCg                <  1:277181/90‑1 (MQ=255)
                                                                              gtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCgg               >  1:3001178/1‑100 (MQ=255)
                                                                              gtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCgg               <  2:2064922/100‑1 (MQ=255)
                                                                               tggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCGTCACTTCTTTATTTTCTGCCggg              <  1:1928784/100‑1 (MQ=255)
                                                                               tcttAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCggg              <  1:2352426/97‑1 (MQ=255)
                                                                                ggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGt             >  1:1599970/1‑100 (MQ=255)
                                                                                ggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGt             >  2:2504770/1‑100 (MQ=255)
                                                                                  tAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTaa           >  2:3328277/1‑100 (MQ=255)
                                                                                   naTGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTaaa          >  1:3138407/2‑100 (MQ=255)
                                                                                     tGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGACCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTGTATTTTCTGCCGGGTAaata        <  1:2860669/100‑1 (MQ=255)
                                                                                      gACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAaataa       >  1:1175297/1‑100 (MQ=255)
                                                                                      gACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAaataa       >  1:865235/1‑100 (MQ=255)
                                                                                       aCGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCgagatcggaag      >  2:277182/1‑90 (MQ=255)
                                                                                       aCGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAaataat      <  1:1286845/100‑1 (MQ=255)
                                                                                       aCGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAATGCACCATCACTTCTTTATTTTCTGCCGGGTAaataat      <  1:2463220/100‑1 (MQ=255)
                                                                                          tATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATgcg   <  2:2944090/100‑1 (MQ=255)
                                                                                           aTCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATgcgc  <  1:2569584/100‑1 (MQ=255)
                                                                                           aTCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATgcgc  >  2:2053678/1‑100 (MQ=255)
                                                                                           aTCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCACTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATgcgc  >  1:250282/1‑100 (MQ=255)
                                                                                                |                                                                                              
TTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGC  >  NC_000913/2191336‑2191526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: