Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,235,656 T→C 63.8% I131T (ATC→ACC)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,6560TC63.8% 161.1 / 152.5 160I131T (ATC→ACC) preAdihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base T (27/31);  new base C (55/47);  total (82/78)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 161 total aligned reads displayed.

TTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGA  >  NC_000913/2235558‑2235754
                                                                                                  |                                                                                                  
ttACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGAtn                                                                                                   <  2:3040660/100‑2 (MQ=255)
  aCCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGa                                                                                                 >  2:2655095/1‑100 (MQ=255)
  aCCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGa                                                                                                 >  1:438739/1‑100 (MQ=255)
   cccGGACAAAGTATTGATCGATTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAg                                                                                                >  1:2068052/1‑100 (MQ=255)
    ccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg                                                                                                <  1:2345066/99‑1 (MQ=255)
     cGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgtg                                                                                              <  1:2524961/100‑1 (MQ=255)
     cGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgtg                                                                                              <  1:1133218/100‑1 (MQ=255)
      ggACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATAGATCggaagag                                                                                            >  1:2991920/1‑94 (MQ=255)
       gACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTa                                                                                            <  2:347605/100‑1 (MQ=255)
        aCAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTaa                                                                                           <  2:493304/100‑1 (MQ=255)
           aaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACtt                                                                                        >  1:1488122/1‑100 (MQ=255)
           aaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACtt                                                                                        >  1:384447/1‑100 (MQ=255)
             gTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTtct                                                                                      <  2:2350766/100‑1 (MQ=255)
               aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTcc                                                                                    <  2:1542161/100‑1 (MQ=255)
               aTTGATCGCTTCGACCATGGGGGAACATGAGCAGCAATGTGAGGAGCTGGCGCGCCTGGTTACAGAAACTGGCGTGGATATGGCCGAGTCTAACTtct                                                                                      >  1:1525568/1‑98 (MQ=255)
                   aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTc                                                                                <  1:2008262/100‑1 (MQ=255)
                    tCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTcc                                                                               <  1:3216126/100‑1 (MQ=255)
                      gCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAGGCTGGCGCGGATATGATCGAGGGTAACTTCTCCTGTCCg                                                                              >  1:2105139/1‑99 (MQ=255)
                      gCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg                                                                              <  2:3331177/99‑1 (MQ=255)
                      gCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGc                                                                             >  1:2360099/1‑100 (MQ=255)
                       cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCa                                                                            <  2:2983473/100‑1 (MQ=255)
                           gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATg                                                                        >  2:695673/1‑100 (MQ=255)
                           gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATg                                                                        >  2:1785356/1‑100 (MQ=255)
                             tCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGAc                                                                      <  2:392602/100‑1 (MQ=255)
                             tCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGAc                                                                      >  2:761504/1‑100 (MQ=255)
                              cATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACt                                                                     <  2:862134/100‑1 (MQ=255)
                                tGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTtc                                                                   <  2:605364/100‑1 (MQ=255)
                                tGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTtc                                                                   <  1:1320894/100‑1 (MQ=255)
                                tGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtc                                                                   <  2:1142591/100‑1 (MQ=255)
                                 gggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct                                                                  >  2:1398044/1‑100 (MQ=255)
                                  ggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtctc                                                                 >  2:2099446/1‑100 (MQ=255)
                                     gAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                              >  2:2267578/1‑100 (MQ=255)
                                     gAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                              >  2:3362279/1‑100 (MQ=255)
                                       aaaTGAGTAGCCATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTCTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                                            >  1:1776156/1‑100 (MQ=255)
                                       aaaTGAGCATCAATCTGATTAGCTGGCGCTCCTGTTTCAAGAATCTGGCGCTCATATTTCCGAATGTAACTTCTCTTGTCCGCAAATGACTTCTCATGCg                                                            <  1:3167051/100‑1 (MQ=255)
                                       aaaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                                            >  1:46600/1‑100 (MQ=255)
                                         aTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCACATGACTTCTCATGCGAt                                                          >  2:2933103/1‑100 (MQ=255)
                                          tGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATg                                                         >  2:2891995/1‑100 (MQ=255)
                                          tGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATg                                                         <  2:166096/100‑1 (MQ=255)
                                           gAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATgg                                                        >  2:2806746/1‑100 (MQ=255)
                                           gAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATgg                                                        >  2:1105699/1‑100 (MQ=255)
                                            agcagcAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGCGTGTAACTTCTCCTGTCCGCAAATGAATTCTCATGCGATggg                                                       >  1:1509825/1‑100 (MQ=255)
                                            agcagcAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATggg                                                       <  1:3022130/100‑1 (MQ=255)
                                            agcagcAATGGGAGGAGCTAGAGCGCCGGTTGCAAGAAGCTGGAGCGGAGAGGACCGAGTGTAACGCCTCCTGTGTGCAAATGACTTCTCAAGCCATg                                                         >  1:2846063/1‑98 (MQ=255)
                                             ttccgatctggaggaGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGt                                                      <  2:1002503/91‑1 (MQ=255)
                                             gcagcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGt                                                      >  1:1949635/1‑100 (MQ=255)
                                              cagcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTa                                                     >  2:1255366/1‑100 (MQ=255)
                                              cagcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTa                                                     <  1:3337620/100‑1 (MQ=255)
                                                gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                   <  2:2562563/100‑1 (MQ=255)
                                                gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                   <  2:3276649/100‑1 (MQ=255)
                                                 caATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCACATGACTTCTCATGCGATGGGTAGCg                                                  >  1:1301745/1‑100 (MQ=255)
                                                  aaTGGGAGGGGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGa                                                 <  1:2136029/100‑1 (MQ=255)
                                                  aaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGa                                                 >  2:1336833/1‑100 (MQ=255)
                                                     gggAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACATCTCCTGTCAGCAAATGACTTCTCATGCGATGGGTAGCGCTGt                                              >  2:1470384/1‑100 (MQ=255)
                                                      ggaggaGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAgatcggaa                                             >  1:1002500/1‑93 (MQ=255)
                                                        aggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCgg                                           >  1:2246098/1‑100 (MQ=255)
                                                        aggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCgg                                           <  1:1009307/100‑1 (MQ=255)
                                                           agCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCa                                        >  2:1725791/1‑100 (MQ=255)
                                                            gCTGGCGCGCGTGGTGAAAGTAGCTGGCGCGGATATGATCGAGTGTAAGTTCTCCTGTCCGCAAATGACTGCTCATGCGATGGGTAGCGa                                                 <  1:915886/90‑1 (MQ=255)
                                                               ggCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGc                                    >  2:2129986/1‑100 (MQ=255)
                                                               ggCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCTAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGc                                    >  1:1818744/1‑100 (MQ=255)
                                                               ggCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGc                                    >  2:1614206/1‑100 (MQ=255)
                                                                  gcgcCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCg                                 <  2:276510/100‑1 (MQ=255)
                                                                   cgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCgg                                <  1:1484754/100‑1 (MQ=255)
                                                                      cTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGc                             <  1:2925926/100‑1 (MQ=255)
                                                                       tGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCt                            <  1:2752893/100‑1 (MQ=255)
                                                                       tGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCt                            >  1:1712990/1‑100 (MQ=255)
                                                                       tGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCt                            >  1:1648986/1‑100 (MQ=255)
                                                                        ggTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTg                           >  1:9433/1‑100 (MQ=255)
                                                                         gTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg                          <  2:197407/100‑1 (MQ=255)
                                                                         gTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTg                           >  1:620702/1‑99 (MQ=255)
                                                                         gTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTGCTCCTCTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg                          <  1:2162476/100‑1 (MQ=255)
                                                                          tGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                         <  2:2702864/100‑1 (MQ=255)
                                                                          tGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                         <  2:1891389/100‑1 (MQ=255)
                                                                           gCAAGAAGCTGGCGCTGATATGACCGAGTGTAACGTCTCCTGGCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                         <  1:3223919/99‑1 (MQ=255)
                                                                           gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTa                        <  2:2612710/100‑1 (MQ=255)
                                                                            cAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAg                       >  2:613007/1‑100 (MQ=255)
                                                                              agaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCGGGTAGaa                     >  1:3226426/1‑100 (MQ=255)
                                                                               gaagCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaa                    >  1:542125/1‑100 (MQ=255)
                                                                                  gCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAat                 <  2:1269161/100‑1 (MQ=255)
                                                                                  gCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAat                 <  1:1336828/100‑1 (MQ=255)
                                                                                   cTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAata                <  1:135124/100‑1 (MQ=255)
                                                                                     ggCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt              >  2:3197790/1‑100 (MQ=255)
                                                                                     ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt              >  1:2697860/1‑100 (MQ=255)
                                                                                     ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGACGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt              >  1:1211417/1‑100 (MQ=255)
                                                                                      gcgcGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTg             <  2:2221743/100‑1 (MQ=255)
                                                                                       cgcgGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTgn            <  1:3362783/100‑2 (MQ=255)
                                                                                       cgcgGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGACAAAGCCCGGAGCTGGTAGAAAAATAtt              >  1:1641518/1‑98 (MQ=255)
                                                                                       cgcgGATATGACCGAGTGTAATTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGAAGCTGGTAGAAAAATATTGt            >  1:198236/1‑100 (MQ=255)
                                                                                       cgcgGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGt            >  2:2461514/1‑100 (MQ=255)
                                                                                       cgcgGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGt            <  1:577896/100‑1 (MQ=255)
                                                                                           gATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCggg        >  2:2467497/1‑100 (MQ=255)
                                                                                           gATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCggg        <  1:1614199/100‑1 (MQ=255)
                                                                                           gATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCgg         <  1:1130476/99‑1 (MQ=255)
                                                                                              atGACCGAGTGTAACTTCTACTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAg     <  1:1406611/100‑1 (MQ=255)
                                                                                               tGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGt    >  1:2365555/1‑100 (MQ=255)
                                                                                               tGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGt    >  2:1523383/1‑100 (MQ=255)
                                                                                                gATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTg   >  2:738059/1‑100 (MQ=255)
                                                                                                 aTCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGa  >  1:2424940/1‑100 (MQ=255)
                                                                                                 aTCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGa  >  2:3274553/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
TTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGA  >  NC_000913/2235558‑2235754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: