Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,278,298 C→T 100% intergenic (‑61/+272) yejG ← / ← bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,278,2980CT98.4% 425.1 / ‑2.5 127intergenic (‑61/+272)yejG/bcruncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (64/60);  total (65/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 133 total aligned reads displayed.

GCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTG  >  NC_000913/2278201‑2278396
                                                                                                 |                                                                                                  
gCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaa                                                                                                  <  1:3310355/100‑1 (MQ=255)
 cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaac                                                                                                 <  1:3197770/100‑1 (MQ=255)
 cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaac                                                                                                 <  2:107609/100‑1 (MQ=255)
 cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaac                                                                                                 >  1:369513/1‑100 (MQ=255)
    tCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACGCATCATTAACAAt                                                                                              <  1:3318757/100‑1 (MQ=255)
     cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                             >  2:2311666/1‑100 (MQ=255)
      gATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATcc                                                                                            >  2:1739488/1‑100 (MQ=255)
       aTGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATccn                                                                                           <  2:1910566/100‑2 (MQ=255)
       aTGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCg                                                                                           >  1:416453/1‑100 (MQ=255)
        tGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGt                                                                                          >  2:1170267/1‑100 (MQ=255)
         ggACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTc                                                                                         <  2:264365/100‑1 (MQ=255)
         ggACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTc                                                                                         >  2:1203050/1‑100 (MQ=255)
         ggACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTc                                                                                         >  1:668201/1‑100 (MQ=255)
           aCGATTGAGAGTTGTAATGAAGTCACTTACTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACACCAATTTTAACACATCATTAACAATCCGTc                                                                                         <  2:2080970/98‑1 (MQ=255)
           aCGATATAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTGTACATCAATTTTAACACATCATTAACAATCCGTcgt                                                                                       <  1:3078548/100‑3 (MQ=255)
            cGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCttt                                                                                      <  1:757467/100‑1 (MQ=255)
            cGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCttt                                                                                      <  1:1076154/100‑1 (MQ=255)
            cGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCttt                                                                                      >  2:3157061/1‑100 (MQ=255)
             gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                     <  1:1016405/100‑1 (MQ=255)
              aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                                    <  1:2477431/100‑1 (MQ=255)
              aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                                    >  2:1235395/1‑100 (MQ=255)
                tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                                  <  1:2787796/100‑1 (MQ=255)
                tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                                  <  1:1143589/100‑1 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                                 <  2:668204/100‑1 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                                 >  1:1951738/1‑100 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                                 >  1:1011200/1‑100 (MQ=255)
                   gatttGTAATGAAGTCACTTAGTTTCCCCGGAAGCACATTCACATCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGa                                                                               <  1:2333082/97‑1 (MQ=255)
                   gagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGa                                                                               <  1:309998/100‑1 (MQ=255)
                   gagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGa                                                                               >  2:1670033/1‑100 (MQ=255)
                    agTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGAt                                                                              >  1:1295239/1‑100 (MQ=255)
                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                            <  1:2599342/100‑1 (MQ=255)
                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                            <  1:1937786/100‑1 (MQ=255)
                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                            <  2:2228952/100‑1 (MQ=255)
                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                            <  2:1549851/100‑1 (MQ=255)
                       tGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGt                                                                           >  2:3282412/1‑100 (MQ=255)
                       tGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGt                                                                           <  2:1520079/100‑1 (MQ=255)
                         tAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTaa                                                                         <  1:1546631/100‑1 (MQ=255)
                         tAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTaa                                                                         >  1:3171437/1‑100 (MQ=255)
                          aaTGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTaaa                                                                        >  2:1141151/1‑100 (MQ=255)
                            tGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGa                                                                      >  1:2237886/1‑100 (MQ=255)
                             gAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAt                                                                     <  2:509085/100‑1 (MQ=255)
                              aaGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                                    <  1:2986421/100‑1 (MQ=255)
                              aaGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                                    <  2:3248140/100‑1 (MQ=255)
                                gTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGa                                                                  >  2:3325822/1‑100 (MQ=255)
                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                                <  1:148760/100‑1 (MQ=255)
                                   aCTTATTTTCCCTGGAAGCACATTCACTTCACAATTTGTTTATATCAATATTAACACATCATTAACAATCCGTTTTTATACAGATCGTACAGAtt                                                                    <  1:1573772/95‑1 (MQ=255)
                                   aCTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGc                                                               >  1:1182951/1‑100 (MQ=255)
                                      tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTc                                                            >  2:2536424/1‑100 (MQ=255)
                                        ttttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGc                                                          >  2:175023/1‑100 (MQ=255)
                                         tttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGcc                                                         <  2:1079651/100‑1 (MQ=255)
                                          ttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGcc                                                         <  2:3171464/99‑1 (MQ=255)
                                           tCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTg                                                       <  2:35613/100‑1 (MQ=255)
                                           tCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTg                                                       <  2:604466/100‑1 (MQ=255)
                                             cccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCg                                                     >  2:1070402/1‑100 (MQ=255)
                                             cccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCg                                                     <  2:2477227/100‑1 (MQ=255)
                                             cccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCg                                                     >  2:269916/1‑100 (MQ=255)
                                                ggAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTgg                                                  <  2:1486934/100‑1 (MQ=255)
                                                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                                 <  2:734147/100‑1 (MQ=255)
                                                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                                 <  1:1541687/100‑1 (MQ=255)
                                                  aaGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGcc                                                <  1:3157034/100‑1 (MQ=255)
                                                  aaGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGcc                                                <  1:2213247/100‑1 (MQ=255)
                                                  aaGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGcc                                                <  2:488108/100‑1 (MQ=255)
                                                   aGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGcc                                                <  1:2357244/99‑1 (MQ=255)
                                                    gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                              <  2:1503642/100‑1 (MQ=255)
                                                     cacaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctct                                             >  2:2810765/1‑100 (MQ=255)
                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                          <  2:241312/100‑1 (MQ=255)
                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                          <  2:3196112/100‑1 (MQ=255)
                                                         ttcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATgg                                         >  2:2758103/1‑100 (MQ=255)
                                                         ttcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATgg                                         >  1:2662803/1‑100 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                        <  2:2049636/100‑1 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                        <  1:3095425/100‑1 (MQ=255)
                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                      >  1:3169667/1‑100 (MQ=255)
                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                      >  2:1154705/1‑100 (MQ=255)
                                                             cttcccAATTTGTTTACATCAATTTTAACCCATCATTAACAATCCGTCTCTATACAGTTCCTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                     <  1:2023187/100‑1 (MQ=255)
                                                             cttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                     >  2:423108/1‑100 (MQ=255)
                                                              ttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTg                                    <  2:1676031/100‑1 (MQ=255)
                                                               tcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGa                                   >  1:2215213/1‑100 (MQ=255)
                                                                  caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                                >  2:486639/1‑100 (MQ=255)
                                                                  caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                                >  1:2999475/1‑100 (MQ=255)
                                                                  caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                  >  1:766689/1‑98 (MQ=255)
                                                                   aaTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTa                               <  1:2810742/100‑1 (MQ=255)
                                                                    aTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTaa                              >  1:3361052/1‑100 (MQ=255)
                                                                     tttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAg                             <  2:1442013/100‑1 (MQ=255)
                                                                     tttGTCTACATCAATTTTAACATGTCGTGAACAATCCGTCTTTATACAGACGGTAATGATGGAGGCCTTGCCTGCGGTACCTCGGTGAGTCTGATTAAAg                             <  1:3206203/100‑1 (MQ=255)
                                                                      ttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGt                            <  1:1030571/100‑1 (MQ=255)
                                                                      ttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGt                            >  1:1580776/1‑100 (MQ=255)
                                                                       tGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                           <  2:220192/100‑1 (MQ=255)
                                                                          ttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAata                        >  2:2497986/1‑100 (MQ=255)
                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGCTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTCATATCTg                    >  1:319577/1‑100 (MQ=255)
                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                    >  2:3109216/1‑100 (MQ=255)
                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                    >  2:2687430/1‑100 (MQ=255)
                                                                                cAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg                  >  2:2688817/1‑100 (MQ=255)
                                                                                 aaTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCgg                 >  2:2497484/1‑100 (MQ=255)
                                                                                 aaTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCgg                 <  1:3282384/100‑1 (MQ=255)
                                                                                  ttccgatctcatcatTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg                <  2:2278779/91‑1 (MQ=255)
                                                                                        acacATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGt          >  1:1743795/1‑100 (MQ=255)
                                                                                         cacaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTc         >  1:2993111/1‑100 (MQ=255)
                                                                                           catcatTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCgggagatcggaa       >  1:2278763/1‑91 (MQ=255)
                                                                                           catcatTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAc       >  1:2543555/1‑100 (MQ=255)
                                                                                                tcaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTg  <  2:2413942/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
GCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTG  >  NC_000913/2278201‑2278396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: