Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,311,616 C→T 15.5% intergenic (‑82/+30) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,311,6160CT15.5% 340.8 / 44.0 135intergenic (‑82/+30)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (55/59);  new base T (7/14);  total (62/73)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 141 total aligned reads displayed.

GGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG  >  NC_000913/2311517‑2311715
                                                                                                   |                                                                                                   
ggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGccc                                                                                                     >  2:628170/1‑100 (MQ=255)
      gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAgg                                                                                                >  1:2024934/1‑99 (MQ=255)
      gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGc                                                                                               <  2:2781867/100‑1 (MQ=255)
      gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGc                                                                                               >  2:3199791/1‑100 (MQ=255)
      gCTTATGTCCATACGTGATTATCCTCATGAGAACGGTCGAAAGAGTACACCAAAAAACTGAGTTTGAACGCAAAAAAAAAAGAAAAAAGGGCCCGCAGGc                                                                                               <  2:2669961/100‑1 (MQ=255)
      gCTGATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGc                                                                                               <  2:2785529/100‑1 (MQ=255)
       ctcaTTTCCATACGAGATTATCCTCATAAAAACAGAAAAAAGAGAAAAAAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGcc                                                                                              <  1:1944370/97‑1 (MQ=255)
       cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTGCGCTGAAAACAATGAAAAAAGGGCCCGCAGGcc                                                                                              <  2:502589/100‑1 (MQ=255)
        ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGccc                                                                                             <  2:227146/100‑1 (MQ=255)
           tttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCttt                                                                                          >  1:1032033/1‑100 (MQ=255)
            ttCCATAGGTGATTATCCTCATGCGAACGGTCGCAAAAAAAAAAAAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCtttt                                                                                         <  1:2734876/100‑2 (MQ=255)
            ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTg                                                                                         <  1:818613/100‑1 (MQ=255)
             tCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGt                                                                                        <  2:2124508/100‑1 (MQ=255)
               cATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAACGGCCTGCGGGCCCTTTTTTc                                                                                      <  1:2073100/100‑1 (MQ=255)
                aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGGGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                     <  2:2535160/100‑1 (MQ=255)
                aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTca                                                                                     <  1:2145200/100‑2 (MQ=38)
                aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTc                                                                                      <  1:3291623/99‑1 (MQ=255)
                aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                     >  2:2023252/1‑100 (MQ=255)
                aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                     >  2:2022948/1‑100 (MQ=255)
                aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                     <  1:1915928/100‑1 (MQ=255)
                aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                     >  1:119005/1‑100 (MQ=255)
                 tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcat                                                                                    <  1:3064029/100‑3 (MQ=38)
                 tACGTGAGTATCCTCATGCGAACGGTCGCAAGGGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTc                                                                                      <  1:1780884/98‑1 (MQ=255)
                 tACGGGAGTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcat                                                                                    <  2:60756/100‑3 (MQ=38)
                  aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                   <  2:243507/100‑1 (MQ=255)
                  aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                   <  2:389323/100‑1 (MQ=255)
                  aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                   <  1:1415881/100‑1 (MQ=255)
                  aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                   <  2:2775011/100‑1 (MQ=255)
                  aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                   <  2:868569/100‑1 (MQ=255)
                   cGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGata                                                                                  <  2:2504991/100‑1 (MQ=255)
                   cGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGata                                                                                  >  1:2584781/1‑100 (MQ=255)
                     tGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATc                                                                                >  2:1470616/1‑100 (MQ=255)
                     tGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATc                                                                                <  1:3365130/100‑1 (MQ=255)
                      gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcattgttt                                                                               <  1:1932530/100‑8 (MQ=25)
                      gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcattgttt                                                                               <  2:1172717/100‑8 (MQ=25)
                      gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcattgttt                                                                               <  1:1366149/100‑8 (MQ=25)
                       aTTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCaa                                                                              >  1:2546582/1‑100 (MQ=255)
                        ttATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcattgttttc                                                                             <  2:1236792/100‑10 (MQ=25)
                         tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcattgtttt                                                                              <  2:1499503/98‑9 (MQ=25)
                          aTCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCg                                                                           >  2:1848530/1‑100 (MQ=255)
                          aTCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCg                                                                           <  1:914493/100‑1 (MQ=255)
                           tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCga                                                                          <  2:865910/100‑1 (MQ=255)
                            ccTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCgag                                                                         <  1:2974738/100‑1 (MQ=255)
                            ccTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCgag                                                                         <  2:1539931/100‑1 (MQ=255)
                             cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCgaga                                                                        <  1:2627211/100‑1 (MQ=255)
                             cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCgaga                                                                        >  1:625455/1‑100 (MQ=255)
                              tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGAt                                                                       >  1:1979364/1‑100 (MQ=255)
                               cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGAtt                                                                      <  1:1579929/100‑1 (MQ=255)
                               cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGAtt                                                                      >  2:295270/1‑100 (MQ=255)
                                 tGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAg                                                                    <  1:2315234/100‑1 (MQ=255)
                                   cGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAATAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGaa                                                                  >  1:2160712/1‑100 (MQ=255)
                                            gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAc                                                         >  1:1130845/1‑100 (MQ=255)
                                            gCAAGAGTACACCAAAAAACTGAGTTGGTACGATGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAc                                                         <  1:1654643/100‑1 (MQ=255)
                                             cAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAcc                                                        >  2:123699/1‑100 (MQ=255)
                                                  gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAcc                                                   <  2:2039861/100‑1 (MQ=255)
                                                    acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCa                                                 >  2:2602010/1‑100 (MQ=255)
                                                     cacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCag                                                >  1:3178845/1‑100 (MQ=255)
                                                     cacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCag                                                >  2:1874842/1‑100 (MQ=255)
                                                      acCAAAAAACTGAGTTTGTCCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGGTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCaga                                               <  1:1142021/100‑1 (MQ=255)
                                                       ccAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCagag                                              >  1:1301149/1‑100 (MQ=255)
                                                       acAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAAGGCACGCAGGACCTTTGTTCTATATCAATATAGATTTGAACTGATAAATAAGActaata                                               >  1:377653/2‑94 (MQ=255)
                                                            aaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACg                                         >  1:2775452/1‑100 (MQ=255)
                                                            aaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACg                                         >  2:1480563/1‑100 (MQ=255)
                                                                tGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGt                                     >  2:1665579/1‑100 (MQ=255)
                                                                 gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTATTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGtt                                    >  1:568972/1‑100 (MQ=255)
                                                                  aGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                   >  1:170418/1‑100 (MQ=255)
                                                                    tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATc                                 <  2:562183/100‑1 (MQ=255)
                                                                      tGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAg                               >  2:579300/1‑100 (MQ=255)
                                                                      tGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAg                               <  2:2205315/100‑1 (MQ=255)
                                                                      tGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTATTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAg                               <  2:568975/100‑1 (MQ=255)
                                                                        tACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtg                             <  2:1301153/100‑1 (MQ=255)
                                                                         aCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtgt                            <  2:3022657/100‑1 (MQ=255)
                                                                          cGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                           <  1:818729/100‑1 (MQ=255)
                                                                          cGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCAATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                           <  2:2305457/100‑1 (MQ=255)
                                                                           gCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTg                          <  2:13661/100‑1 (MQ=255)
                                                                              gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                       <  2:2620708/100‑1 (MQ=255)
                                                                              gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCACACCCAGAGCTACGATGTTATCAGTGTTGATg                       <  2:2283218/100‑1 (MQ=255)
                                                                               aaaaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                      >  2:1299517/1‑100 (MQ=255)
                                                                                    atcgaacaaaGGGCCTGCGGGCCCTTTTTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCg                 >  2:1107912/8‑100 (MQ=37)
                                                                                    aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCg                 >  2:2295392/1‑100 (MQ=255)
                                                                                     aTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGt                <  2:1036903/100‑1 (MQ=255)
                                                                                      tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc               >  2:1118780/1‑100 (MQ=255)
                                                                                      tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc               >  2:2455104/1‑100 (MQ=255)
                                                                                      tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc               <  2:404948/100‑1 (MQ=255)
                                                                                       gAACAAAGGGCCTGCGGGCCCTTTTTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCa              >  1:1302245/1‑100 (MQ=38)
                                                                                       gAACAAAGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCa              >  1:3271327/1‑100 (MQ=38)
                                                                                       gAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCa              >  2:242301/1‑100 (MQ=255)
                                                                                        aacaaaGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCAc             >  2:303203/4‑100 (MQ=38)
                                                                                        aaaaaaGGGCCCGCCGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc               >  1:1219415/1‑98 (MQ=255)
                                                                                        aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCAc             >  2:754970/1‑100 (MQ=255)
                                                                                        aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCAc             <  2:2510694/100‑1 (MQ=255)
                                                                                         aaaaaGGGCTCGTGAGCCCTTGTTTCGAGATCAAGCGAGAGTAGAACTGTTAACCCAGACCCAGAGCTATGATGTTATCAGTGTTGATGCGAGCGTCa              <  1:1325178/98‑1 (MQ=255)
                                                                                         aaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg            >  1:1517691/1‑100 (MQ=255)
                                                                                         aaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACg            <  2:3212591/100‑1 (MQ=255)
                                                                                          caaaGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGa           >  2:755953/2‑100 (MQ=255)
                                                                                                gCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc     <  2:1418937/100‑1 (MQ=255)
                                                                                                gCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc     <  2:2081361/100‑1 (MQ=255)
                                                                                                gCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc     >  1:771465/1‑100 (MQ=255)
                                                                                                 cccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACt    >  1:1505559/1‑100 (MQ=255)
                                                                                                  ccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTg   >  2:1448901/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGG  >  NC_000913/2311517‑2311715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: