Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,352,993 T→A 32.5% I116N (ATC→AAC)  glpA → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,352,9930TA32.5% 152.0 / 124.3 133I116N (ATC→AAC) glpAanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (44/45);  new base A (25/18);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01

Only 100 of 134 total aligned reads displayed.

TCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGA  >  NC_000913/2352895‑2353092
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tCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATc                                                                                                    <  2:2944275/100‑1 (MQ=255)
  aCTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg                                                                                                  <  2:2811929/100‑1 (MQ=255)
   cTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAgc                                                                                                 <  1:548660/100‑1 (MQ=255)
   cTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAgc                                                                                                 >  2:825096/1‑100 (MQ=255)
    tGCGTTGAACTAACCAACGGCCTTTTTATCACCTTTCCGGAAGATGACCTCTCTTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAgc                                                                                                 <  1:831563/99‑1 (MQ=255)
    tGCGTTGAACCAACCATTTGCCTTTTTATTACCCTGCCGGAAGATGACGTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAgcg                                                                                                <  2:875414/100‑1 (MQ=255)
    tGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAgcg                                                                                                <  1:2125209/100‑1 (MQ=255)
    tGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAgcg                                                                                                <  2:1627152/100‑1 (MQ=255)
      cGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCa                                                                                              >  2:3311102/1‑100 (MQ=255)
        ttGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGa                                                                                            >  1:1528462/1‑100 (MQ=255)
         tGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGaa                                                                                           >  1:2439384/1‑100 (MQ=255)
         tGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGaa                                                                                           >  1:2013747/1‑100 (MQ=255)
          gAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAg                                                                                          >  1:1414492/1‑100 (MQ=255)
           aaccaaccAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGc                                                                                         <  1:116547/100‑1 (MQ=255)
           aaccaaccAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGc                                                                                         <  1:1420987/100‑1 (MQ=255)
            accaaccaACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCt                                                                                        <  2:1736368/100‑1 (MQ=255)
            accaaccaACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGc                                                                                         >  2:2077222/1‑99 (MQ=255)
               aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCtata                                                                                     >  2:1318115/1‑100 (MQ=255)
                 ccaacGGCCTGATTATCACCCTGCCGGAAGACGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGa                                                                                   >  1:2599808/1‑100 (MQ=255)
                   aacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGAcc                                                                                 >  1:2892402/1‑100 (MQ=255)
                   aacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGAcc                                                                                 >  1:1788727/1‑100 (MQ=255)
                      ggtcTGTTGATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCg                                                                               <  2:280430/96‑1 (MQ=255)
                      ggCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCgc                                                                              <  2:3057213/100‑1 (MQ=255)
                       gCCTGTTTATGACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGGAGTAGGGATCAGCGCAGAAGCTATAGACCCgca                                                                             <  1:2234124/100‑1 (MQ=255)
                       gCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgca                                                                             >  2:1634851/1‑100 (MQ=255)
                       gCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgca                                                                             <  2:3272550/100‑1 (MQ=255)
                       gCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCgca                                                                             >  1:642522/1‑100 (MQ=255)
                         cTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgcagc                                                                           <  1:695327/100‑1 (MQ=255)
                            tttATCACCCTGCCGGAAGATGACCTCTCCTTCCTGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCgcagc                                                                           >  1:2968123/1‑97 (MQ=255)
                            tttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgcagca                                                                          <  1:530908/98‑1 (MQ=255)
                            tttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAg                                                                        <  2:344551/100‑1 (MQ=255)
                               aTCACCCTGCCGGAAGATGACCTCTCCTTCCAGACCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCACAGCAAgcgc                                                                     >  1:2220848/1‑100 (MQ=255)
                                tCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcgcg                                                                    >  1:1759318/1‑100 (MQ=255)
                                tCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcgcg                                                                    >  2:843494/1‑100 (MQ=255)
                                 cACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcgcgc                                                                   >  1:2633415/1‑100 (MQ=255)
                                  aCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCa                                                                  <  1:3301084/100‑1 (MQ=255)
                                   cccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAt                                                                 >  2:1149749/1‑100 (MQ=255)
                                   cccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAt                                                                 >  2:1083919/1‑100 (MQ=255)
                                    ccTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAtt                                                                >  2:475578/1‑100 (MQ=255)
                                        ccGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCg                                                            >  2:922322/1‑100 (MQ=255)
                                        ccGGAAGATCACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATc                                                             <  1:2427789/99‑1 (MQ=255)
                                          ggAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGaa                                                          >  1:3278410/1‑100 (MQ=255)
                                           gAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAAc                                                         <  1:1066481/100‑1 (MQ=255)
                                            aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAAc                                                         <  2:192178/99‑1 (MQ=255)
                                               aTGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGc                                                     <  2:2004538/100‑1 (MQ=255)
                                                 gagctctCCTTCCAGACCACTTTGATACGCGCAGGCGAAGAAGCAGGGAGCAGCGCAGAAGCTATAGACCCGCATCAATCGCGCAGTATCGAACCAGcc                                                    <  1:1749777/96‑1 (MQ=255)
                                                  aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGcc                                                    <  1:1045585/98‑1 (MQ=255)
                                                  aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGt                                                  >  2:216721/1‑100 (MQ=255)
                                                  aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGt                                                  >  1:1553874/1‑100 (MQ=255)
                                                  aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGt                                                  <  1:3024413/100‑1 (MQ=255)
                                                   ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt                                                 <  2:2256588/100‑1 (MQ=255)
                                                   ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt                                                 >  2:424314/1‑100 (MQ=255)
                                                       tcCTGCCAGGCCACTTTTAGTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTAGCGAACCTGCCGTTAAcc                                             <  1:703125/100‑1 (MQ=255)
                                                        ccTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAAccc                                            >  2:2046092/1‑100 (MQ=255)
                                                         cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg                                           >  2:534783/1‑100 (MQ=255)
                                                         cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg                                           >  2:530297/1‑100 (MQ=255)
                                                         cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg                                           >  1:1150943/1‑100 (MQ=255)
                                                         cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg                                           >  1:1178612/1‑100 (MQ=255)
                                                            ccAGGCCACTTTTATTCGCGCCTGCGAAGAAGCGGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGc                                         <  1:1585428/99‑1 (MQ=255)
                                                              aGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACt                                      <  1:2217583/100‑1 (MQ=255)
                                                                gCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGa                                    >  2:2504953/1‑100 (MQ=255)
                                                                  cACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGAtt                                  >  2:587000/1‑100 (MQ=255)
                                                                    cTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg                                >  2:2458188/1‑100 (MQ=255)
                                                                    cTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg                                <  1:981455/100‑1 (MQ=255)
                                                                    cTTTTATTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAGGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTgg                                <  2:2111273/100‑1 (MQ=255)
                                                                     ttttATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgc                               >  2:2691285/1‑100 (MQ=255)
                                                                      tttATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgcg                              >  2:3159156/1‑100 (MQ=255)
                                                                       ttATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgcgc                             <  1:1051079/100‑1 (MQ=255)
                                                                         aTTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg                           >  2:2964748/1‑100 (MQ=255)
                                                                         aTTCGCGCCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg                           <  2:3093751/100‑1 (MQ=255)
                                                                          ttCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCgg                           >  2:1262582/1‑99 (MQ=255)
                                                                           tCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTg                         <  2:48679/100‑1 (MQ=255)
                                                                            cgcgCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGa                        >  2:1828209/1‑100 (MQ=255)
                                                                             gcgcCTGCGAAGAAGCAGGGATCAGGGCAGTAGCTATAGACCCGCAGCAAGCGCGCACTATCGAACCTGCCGTTAACCCGGCTCTGATTGGCGCgg                           <  1:2094497/96‑1 (MQ=255)
                                                                             gcgcCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGaa                       >  2:1768816/1‑100 (MQ=255)
                                                                              cgcCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGaaa                      >  1:3168313/1‑100 (MQ=255)
                                                                               gcCTGCGCAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAg                     >  1:2361079/1‑100 (MQ=255)
                                                                               gcCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGGCCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAg                     <  1:1255028/100‑1 (MQ=255)
                                                                               gcCTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAg                     >  1:3331159/1‑100 (MQ=255)
                                                                                ccTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGt                    <  2:1988280/100‑1 (MQ=255)
                                                                                ccTGCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGt                    <  1:80791/100‑1 (MQ=255)
                                                                                 cTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGtt                   >  2:2231059/1‑100 (MQ=255)
                                                                                  tGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGtt                   >  1:1037649/1‑99 (MQ=255)
                                                                                  tGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTc                  >  1:859136/1‑100 (MQ=255)
                                                                                  tGCGAAGAAGCAGGGAACAGCGCAGAAGCTATACACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTc                  >  2:1164753/1‑100 (MQ=255)
                                                                                   gCGAAGAAGCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTcc                 >  1:1362869/1‑100 (MQ=255)
                                                                                    cGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCg                <  2:2595027/100‑1 (MQ=255)
                                                                                      aagaagCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGa              >  2:3194422/1‑100 (MQ=255)
                                                                                        gaagCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATg            <  2:2790094/100‑1 (MQ=255)
                                                                                        gaagCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATg            <  1:133905/100‑1 (MQ=255)
                                                                                        gaagCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATg            <  2:2581957/100‑1 (MQ=255)
                                                                                         aagCAGGGATCAGCGCAGAAGCTATAGACCGGCAGCAAGCGCGCGTTCTCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTccctg               >  1:2649843/1‑94 (MQ=255)
                                                                                           ncAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGCTTGGCGCGGTGAAAGTTCCGGATGGc          >  1:3138388/2‑99 (MQ=255)
                                                                                           gCAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCa         <  2:1475304/100‑1 (MQ=255)
                                                                                            cAGGGAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCAc        >  2:2187510/1‑100 (MQ=255)
                                                                                              gggAACAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCg      >  2:1061147/1‑100 (MQ=255)
                                                                                                gATCAGCGCATAAGCTATAGCCCCGCAGCAAGCGCGCATTATCGAACATGTCGTTAACCCGGCACTGATTGTCGCGGTGAAAGTTCCGGATGGCACCGtt    <  1:2917700/100‑1 (MQ=255)
                                                                                                gATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCa         >  1:567674/1‑95 (MQ=255)
                                                                                                gATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGtt    <  2:776053/100‑1 (MQ=255)
                                                                                                 aTCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTg   <  2:1660820/100‑1 (MQ=255)
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TCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGA  >  NC_000913/2352895‑2353092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: