BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,530,925	T→C	68.8%	Q103Q (CAA→CAG)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,530,925	0	T	C	68.8%	186.9 / 112.3	141	Q103Q (CAA→CAG)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base T (18/26); new base C (41/56); total (59/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 144 total aligned reads displayed.

TGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTC  >  NC_000913/2530827‑2531021

tGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGtt                                                                                                 >  2:594659/1‑100 (MQ=255)

tGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGtt                                                                                                 >  2:2915641/1‑100 (MQ=255)

tGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGtt                                                                                                 <  2:821678/100‑1 (MQ=255)

   tCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAc                                                                                              >  1:1007458/1‑100 (MQ=255)

     aGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGAcgc                                                                                            >  2:3323057/1‑100 (MQ=255)

      ggCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGCCCGGTTGAcgcg                                                                                           >  1:3188717/1‑100 (MQ=255)

      ggCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAcgcg                                                                                           >  2:2772480/1‑100 (MQ=255)

       gCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCACTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCgg                                                                                          >  2:3281137/1‑100 (MQ=255)

        cTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGc                                                                                         <  2:53025/100‑1 (MQ=255)

        cTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGc                                                                                         <  1:355672/100‑1 (MQ=255)

          gCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGc                                                                                         <  1:906775/98‑1 (MQ=255)

            ggCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcg                                                                                     >  1:1389042/1‑100 (MQ=255)

             gCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTgcgc                                                                                    <  2:842332/100‑1 (MQ=255)

             gCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTgcgc                                                                                    <  2:3107133/100‑1 (MQ=255)

              cTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCa                                                                                   <  1:1837995/100‑1 (MQ=255)

              cTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCa                                                                                   <  2:2346335/100‑1 (MQ=255)

              cTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCa                                                                                   <  2:3168087/100‑1 (MQ=255)

               tGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAg                                                                                  >  1:952682/1‑100 (MQ=255)

                gATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGa                                                                                 >  1:22589/1‑100 (MQ=255)

                 aTAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGAc                                                                                <  2:1742996/100‑1 (MQ=255)

                  tAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACg                                                                               >  2:856042/1‑100 (MQ=255)

                    ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             >  1:4109/1‑100 (MQ=255)

                    ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             <  1:748308/100‑1 (MQ=255)

                    ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             <  2:697499/100‑1 (MQ=255)

                     gcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCAt                                                                            >  2:1756050/1‑100 (MQ=255)

                     gcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCAt                                                                            >  1:650893/1‑100 (MQ=255)

                      caggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATa                                                                           <  2:22589/100‑1 (MQ=255)

                      caggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATa                                                                           <  2:127284/100‑1 (MQ=255)

                        ggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAg                                                                         <  1:1194794/100‑1 (MQ=255)

                        ggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAg                                                                         >  2:157605/1‑100 (MQ=255)

                        ggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAg                                                                         <  1:3066298/100‑1 (MQ=255)

                          ctgctgCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGc                                                                       <  1:2506661/100‑1 (MQ=255)

                             ctgcACCGGCTGCGCTGGCTGCGGGGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGCCCGTCTCACGCGTCTGCGCAGACGCATAAGGCgg                                                                     <  1:2841939/99‑1 (MQ=255)

                              tgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGtt                                                                   >  2:1679527/1‑100 (MQ=255)

                              tgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGtt                                                                   >  1:1673847/1‑100 (MQ=255)

                               gcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTg                                                                  >  1:2415332/1‑100 (MQ=255)

                               gcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTg                                                                  <  1:792833/100‑1 (MQ=255)

                                cACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTgg                                                                 <  2:2463223/100‑1 (MQ=255)

                                     gCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTg                                                            <  1:837263/100‑1 (MQ=255)

                                      cTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           <  2:2345139/100‑1 (MQ=255)

                                      cTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           <  2:1181726/100‑1 (MQ=255)

                                      cTGCGCTGGATGCGGAGCATGTTGCCGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           <  2:1170881/100‑1 (MQ=255)

                                       tGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCCGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           >  1:1845221/1‑99 (MQ=255)

                                       tGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCTGTTGGTACTGgt                                                          <  2:2227380/100‑1 (MQ=255)

                                       tGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgt                                                          <  2:1202474/100‑1 (MQ=255)

                                          gcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGtt                                                       <  1:1536056/100‑1 (MQ=255)

                                           cTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTAACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      <  2:409955/100‑1 (MQ=255)

                                             ggATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGGGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGGGTTgcg                                                    <  1:2775218/100‑1 (MQ=255)

                                             ggATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcg                                                    >  1:1487653/1‑100 (MQ=255)

                                              gATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcgg                                                   <  1:2153421/100‑1 (MQ=255)

                                               aTGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  <  2:3334262/100‑1 (MQ=255)

                                               aTGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  >  2:530457/1‑100 (MQ=255)

                                                   ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacg                                              >  1:2578489/1‑100 (MQ=255)

                                                   ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacg                                              >  2:1362191/1‑100 (MQ=255)

                                                   ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacg                                              <  2:1901783/100‑1 (MQ=255)

                                                      gCATGTTGCGGCGGTACCTGCGCTTCAGGTGGCTGCTGGACCGGTTGACGCGGCAGCGCAGACGCATAAGGCGGTTGGTACTGGTATTGCGGCgacgga                                            >  1:920412/1‑99 (MQ=255)

                                                        aTGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGa                                         <  2:2887593/100‑1 (MQ=255)

                                                         tGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGag                                        <  2:2608900/100‑1 (MQ=255)

                                                         tGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGag                                        >  2:2177636/1‑100 (MQ=255)

                                                         tGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGag                                        <  2:2941104/100‑1 (MQ=255)

                                                          gTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGagc                                       >  2:1613457/1‑100 (MQ=255)

                                                              cggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGcc                                   <  1:242807/100‑1 (MQ=255)

                                                              cggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAAcc                                   <  2:1095219/100‑1 (MQ=255)

                                                              cggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGcc                                   >  2:3091660/1‑100 (MQ=255)

                                                               gttggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCt                                  >  1:1266452/4‑100 (MQ=255)

                                                               ggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCt                                  <  2:1487659/100‑1 (MQ=255)

                                                                gcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTc                                 >  1:2659692/1‑100 (MQ=255)

                                                                gcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTc                                 <  2:2578509/100‑1 (MQ=255)

                                                                 cggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCa                                >  1:2469670/1‑100 (MQ=255)

                                                                  ggTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCAt                               >  2:2732619/1‑100 (MQ=255)

                                                                    tACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGc                             <  2:661739/100‑1 (MQ=255)

                                                                     aCCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGACTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGc                             >  1:1804336/1‑99 (MQ=255)

                                                                       cTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTcc                          <  1:2530795/100‑1 (MQ=255)

                                                                       cTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTcc                          <  1:2465525/100‑1 (MQ=255)

                                                                        tGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCt                         >  1:146880/1‑100 (MQ=255)

                                                                         gcgcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTg                        >  1:3350511/1‑100 (MQ=255)

                                                                          cgcTTCAGGCGGCTGCTGTAGCGGCTGACGCGGCTGCGGAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGTACGAGCAGCCTCATGCTCCTg                        <  1:2057529/99‑1 (MQ=255)

                                                                          cgcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGCCGGACGAGCAGCCTCATGCTCCTGa                       >  1:769222/1‑100 (MQ=255)

                                                                           gcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAg                      >  1:151458/1‑100 (MQ=255)

                                                                                 ggcggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAg                >  2:973898/1‑100 (MQ=255)

                                                                                 ggcggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAg                <  1:312938/100‑1 (MQ=255)

                                                                                  gcggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCAGAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGc               >  1:776398/1‑100 (MQ=255)

                                                                                   cggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGcc              >  1:2317820/1‑100 (MQ=255)

                                                                                   cggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACCGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGcc              <  1:841022/100‑1 (MQ=255)

                                                                                    ggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGAACGAGCAGCCTCATGCTCCTGAGCGTTAGCCg             <  1:2612063/100‑1 (MQ=255)

                                                                                     gctgctGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCgg            <  1:110245/100‑1 (MQ=255)

                                                                                     gctgctGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCgg            <  2:3188745/100‑1 (MQ=255)

                                                                                     gctgctGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCgg            <  2:3362684/100‑1 (MQ=255)

                                                                                     gctgctGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCgg            <  2:2659714/100‑1 (MQ=255)

                                                                                      ctgctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           >  2:1603512/1‑100 (MQ=255)

                                                                                        gctgGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGc         >  2:765615/1‑100 (MQ=255)

                                                                                        gctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGc         <  2:2769804/100‑1 (MQ=255)

                                                                                        gctgGACCGGCTGACGCGGCGGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGc         <  1:1984114/100‑1 (MQ=255)

                                                                                         ctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCa        >  1:1644196/1‑100 (MQ=255)

                                                                                          tgGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCAt       >  2:93006/1‑100 (MQ=255)

                                                                                           ggACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATg      >  1:3306628/1‑100 (MQ=255)

                                                                                           ggACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATg      >  2:2469775/1‑100 (MQ=255)

                                                                                           ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATg      <  2:1935883/100‑1 (MQ=255)

                                                                                             aCCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGAt    >  1:1720639/1‑100 (MQ=255)

                                                                                               cGGCTGACGCGGCTGCGCAGACAAATAAGGCGCTTGGTACTGGTGTTGCGGCGACAGACGACCAGCCCCATGCTCCTGAGCGTTAGCCGGGGCATg      >  1:1269500/1‑96 (MQ=255)

TGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTC  >  NC_000913/2530827‑2531021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`