Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,564,525 G→A 100% M1M (ATG→ATA) † yffS → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,564,5250GA98.5% 439.3 / ‑2.6 133M1M (ATG→ATA) †yffSCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (65/66);  total (66/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 133 total aligned reads displayed.

TGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCG  >  NC_000913/2564429‑2564619
                                                                                                |                                                                                              
tgATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGt                                                                                             >  1:3164900/1‑100 (MQ=255)
tgATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGt                                                                                             >  2:3220860/1‑100 (MQ=255)
 gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAg                                                                                              >  1:639432/1‑98 (MQ=255)
 gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt                                                                                            >  2:702379/1‑100 (MQ=255)
   tCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTAc                                                                                          >  2:1076786/1‑100 (MQ=255)
        gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAAt                                                                                     >  1:1830085/1‑100 (MQ=255)
           aaTATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCaa                                                                                  <  2:2394909/100‑1 (MQ=255)
           aaTATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCaa                                                                                  <  2:3385348/100‑1 (MQ=255)
               ttCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAtt                                                                              >  2:206320/1‑100 (MQ=255)
                 cccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTg                                                                            >  1:3119265/1‑100 (MQ=255)
                  ccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCTTAAGTTACGAAATCAAAATTTGc                                                                           >  1:2529030/1‑100 (MQ=255)
                  ccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGc                                                                           >  1:1186699/1‑100 (MQ=255)
                   cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCg                                                                          <  1:443818/100‑1 (MQ=255)
                   cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCg                                                                          >  2:443000/1‑100 (MQ=255)
                   cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCg                                                                          <  1:2155739/100‑1 (MQ=255)
                     tCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGAc                                                                        <  1:2241301/100‑1 (MQ=255)
                     tCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGAc                                                                        <  2:2299121/100‑1 (MQ=255)
                     tCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGAc                                                                        <  2:1404714/100‑1 (MQ=255)
                      ccGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACa                                                                       <  1:1112825/100‑1 (MQ=255)
                       cGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACAt                                                                      >  2:3026648/1‑100 (MQ=255)
                        gTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATc                                                                     >  1:798276/1‑100 (MQ=255)
                           aCGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTc                                                                  <  2:1164956/100‑1 (MQ=255)
                           aCGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTc                                                                  <  1:977836/100‑1 (MQ=255)
                             gCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaa                                                                >  2:2765041/1‑100 (MQ=255)
                             cgatctccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaa                                                                <  2:2491574/94‑1 (MQ=255)
                               ttACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAg                                                              <  1:2108716/100‑1 (MQ=255)
                                tACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAgg                                                             >  2:2842230/1‑100 (MQ=255)
                                 aCCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgc                                                            <  1:503073/100‑1 (MQ=255)
                                 aCCCGCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgc                                                            <  1:2654848/100‑1 (MQ=255)
                                  nccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCAATGAGATGGGAGATAAAAATCATAAGATACGAAATCAAAATTTGCGACATCCTCAAAGgcg                                                           >  1:2983852/2‑100 (MQ=255)
                                  cccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg                                                           >  1:2251106/1‑100 (MQ=255)
                                  cccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg                                                           >  1:1455676/1‑100 (MQ=255)
                                  cccTCCACCAATAAGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg                                                           >  2:2278559/1‑100 (MQ=255)
                                   ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAgatcg                                                          >  1:2491555/1‑96 (MQ=255)
                                   ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcgc                                                          <  1:2863473/100‑1 (MQ=255)
                                   ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcgc                                                          <  1:804933/100‑1 (MQ=255)
                                   ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCATCAAAGgcgc                                                          >  1:2812226/1‑100 (MQ=255)
                                     tCCACCAATACGCGTGTTAACCTCCCAATGGATTATATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTg                                                        <  1:566077/100‑1 (MQ=255)
                                      ccaccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGc                                                       >  2:1677263/1‑100 (MQ=255)
                                       caccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCg                                                      <  1:206320/100‑1 (MQ=255)
                                       caccaATACGCGTGTTAACCTCCCAATGGATTCTACGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCg                                                      >  1:3325277/1‑100 (MQ=255)
                                         ccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGAt                                                    <  1:3169543/100‑1 (MQ=255)
                                          caATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATg                                                   <  2:1158850/100‑1 (MQ=255)
                                          caATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATg                                                   <  1:2475568/100‑1 (MQ=255)
                                             tACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGaa                                                >  2:1863995/1‑100 (MQ=255)
                                              aCGCGTGTTAACCTCCCAATGGATTGTATGAGATGGGAGATAAAGATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATgg                                                  <  1:3007966/97‑1 (MQ=255)
                                               cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGaa                                                >  1:147379/1‑98 (MQ=255)
                                               cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAgg                                              <  2:465342/100‑1 (MQ=255)
                                               cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAgg                                              >  1:1040136/1‑100 (MQ=255)
                                               cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAgg                                              >  2:1902104/1‑100 (MQ=255)
                                                  gtgtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACa                                           >  2:2131915/1‑100 (MQ=255)
                                                     ttAATCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAAGCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAatn                                        <  1:435024/100‑2 (MQ=255)
                                                     ttAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAata                                        <  2:2541386/100‑1 (MQ=255)
                                                     ttAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAata                                        >  2:1497784/1‑100 (MQ=255)
                                                     ttAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAata                                        <  2:1284865/100‑1 (MQ=255)
                                                     ttAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATACTCAAAGGCGCTGCGATGGAAGGACAata                                        >  2:1888269/1‑100 (MQ=255)
                                                     ttAACCTCCCAATGGATTCTATGAGAGGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAata                                        <  2:266392/100‑1 (MQ=255)
                                                       aaCCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata                                      >  2:3295322/1‑100 (MQ=255)
                                                        aCCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATaa                                     >  1:1572998/1‑100 (MQ=255)
                                                         gcTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAg                                    >  1:283042/2‑100 (MQ=255)
                                                          cTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAgg                                   >  1:2922204/1‑100 (MQ=255)
                                                           tCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg                                  <  1:796994/100‑1 (MQ=255)
                                                           tCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg                                  >  1:1365866/1‑100 (MQ=255)
                                                           tCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAgg                                   >  1:1797672/1‑99 (MQ=255)
                                                             ccAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgc                                 <  1:3009627/99‑1 (MQ=255)
                                                             ccAACGGAGTCTATTAGATGGGAGATAAAAAACATAAGTAACGAAATAAAAAATTGTGACAACCTCAAAGGCGCTGTGATGGAAGGACAATATAAGGgc                                 <  1:2124620/99‑1 (MQ=255)
                                                              cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc                               >  1:1392225/1‑100 (MQ=255)
                                                              cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc                               <  2:441450/100‑1 (MQ=255)
                                                               aaTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                              <  1:1235738/100‑1 (MQ=255)
                                                                aTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc                               >  1:2765656/1‑98 (MQ=255)
                                                                  ggATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCaa                           >  1:2585727/1‑100 (MQ=255)
                                                                   gATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAAc                          <  1:2932809/100‑1 (MQ=255)
                                                                    aTTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCTACATCCTCAAAGGCGCTGCGATGTAAGGACAATATAAGGGCGCTCAACg                         <  1:1857065/100‑1 (MQ=255)
                                                                    aTTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACg                         >  1:1210879/1‑100 (MQ=255)
                                                                     ttCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGt                        <  1:2739286/100‑1 (MQ=255)
                                                                      tCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTg                       <  2:2549779/100‑1 (MQ=255)
                                                                       cTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg                      >  2:250239/1‑100 (MQ=255)
                                                                        tATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAACATAAGGGCGCTCAACGTggg                     <  1:2071371/100‑1 (MQ=255)
                                                                         aTGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgggg                    <  2:2512782/100‑1 (MQ=255)
                                                                           gagaTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGcc                  <  2:2110185/100‑1 (MQ=255)
                                                                             gaTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCaa                >  1:587442/1‑100 (MQ=255)
                                                                              aTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCaaa               >  1:1011362/1‑100 (MQ=255)
                                                                               tGGGAGATAAACCTCATAAGTTACAACACCAAACTTTGCGACATCCTCAAACGCGCTGGGAAGAAACGACAACATACGGGCGCTCAACGTGGGGCAAAAt              <  1:1518574/100‑1 (MQ=255)
                                                                               tGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAAt              <  1:1019445/100‑1 (MQ=255)
                                                                                 ggAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGc            >  2:2732056/1‑100 (MQ=255)
                                                                                 ggAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGc            >  2:767564/1‑100 (MQ=255)
                                                                                  gagaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGTACAATATAAGGGCGCTCAACGTGGGGCCAAATGCg           <  2:840670/100‑1 (MQ=255)
                                                                                  gagaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCg           >  1:1358597/1‑100 (MQ=255)
                                                                                   agaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGTGGCCAAATGCga          <  2:2232240/100‑1 (MQ=255)
                                                                                   agaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCga          <  1:928429/100‑1 (MQ=255)
                                                                                   agaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCga          <  1:344246/100‑1 (MQ=255)
                                                                                    gaTAAAAATCATAGGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGTACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgag         <  1:3273641/100‑1 (MQ=255)
                                                                                     aTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagg        >  1:2409653/1‑100 (MQ=255)
                                                                                     aTAAAAAGCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagg        <  1:2048022/100‑1 (MQ=255)
                                                                                      tAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagga       <  2:3070335/100‑1 (MQ=255)
                                                                                      tAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagga       >  1:2389296/1‑100 (MQ=255)
                                                                                      tAAAAATCATAAGTTACGAAATCAAAATTTGCGACATACTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagga       >  1:1547843/1‑100 (MQ=255)
                                                                                           aTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCg  <  1:30350/100‑1 (MQ=255)
                                                                                           aTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCg  <  1:2186813/100‑1 (MQ=255)
                                                                                           aTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCg  <  1:1439015/100‑1 (MQ=255)
                                                                                                |                                                                                              
TGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCG  >  NC_000913/2564429‑2564619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: