BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,600,288	C→T	100%	S128L (TCG→TTG)	gcvR →	transcriptional repressor, regulatory protein accessory to GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,600,288	0	C	T	100.0%	522.9 / NA	151	S128L (TCG→TTG)	gcvR	transcriptional repressor, regulatory protein accessory to GcvA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (63/88); total (63/88)

Only 100 of 155 total aligned reads displayed.

GTCTGGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGCA  >  NC_000913/2600191‑2600384

gtctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTtgc                                                                                                <  1:986326/100‑1 (MQ=255)

  ctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTgcgc                                                                                              <  2:851355/100‑1 (MQ=255)

   tgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgca                                                                                             >  1:1174802/1‑100 (MQ=255)

   tgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgca                                                                                             <  1:1207616/100‑1 (MQ=255)

   tgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgca                                                                                             <  2:2996368/100‑1 (MQ=255)

   tgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgca                                                                                             <  1:828880/100‑1 (MQ=255)

   tgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgca                                                                                             <  1:1968555/100‑1 (MQ=255)

   tgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATCGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgca                                                                                             >  1:2081327/1‑100 (MQ=255)

    gggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcac                                                                                            >  2:831599/1‑100 (MQ=255)

    gggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcac                                                                                            >  2:2324952/1‑100 (MQ=255)

     ggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcacg                                                                                           >  1:1865229/1‑100 (MQ=255)

      gTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcacgc                                                                                          >  2:1307822/1‑100 (MQ=255)

      gTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcacgc                                                                                          <  2:3087103/100‑1 (MQ=255)

      gTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcacgc                                                                                          >  2:1405707/1‑100 (MQ=255)

       tgcAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCAGTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcacgca                                                                                         <  1:862622/98‑1 (MQ=255)

            gTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcacc                                                                                    <  2:3142370/100‑4 (MQ=255)

            gTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcacc                                                                                    <  1:1428345/100‑4 (MQ=255)

               gATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACctgctg                                                                                 >  1:459839/1‑100 (MQ=255)

                 tgtgGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCATTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcaccag                                                                                  <  1:2164200/97‑6 (MQ=255)

                 tgtgGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTACTGaa                                                                               <  1:178334/100‑1 (MQ=255)

                    ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAAt                                                                            >  2:3287138/1‑100 (MQ=255)

                    ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAAt                                                                            >  1:335505/1‑100 (MQ=255)

                    ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGCTGCGCACGCAACCTGCTGAAAAt                                                                            >  1:1575402/1‑100 (MQ=255)

                     gCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATg                                                                           <  1:1750939/100‑1 (MQ=255)

                        gACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAAc                                                                        <  2:2446767/100‑1 (MQ=255)

                         aCTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACg                                                                       <  1:190727/100‑1 (MQ=255)

                            ccccGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGc                                                                    >  1:2884595/1‑100 (MQ=255)

                                gCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTgcg                                                                <  1:2362185/100‑1 (MQ=255)

                                gCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTgc                                                                 <  2:1386259/99‑1 (MQ=255)

                                  aTTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCa                                                              >  2:2064588/1‑100 (MQ=255)

                                    ttAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGt                                                            <  1:3109982/100‑1 (MQ=255)

                                    ttAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGt                                                            <  2:2050907/100‑1 (MQ=255)

                                    ttAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGAGGAGCTGATGTTGCGCACGCAACCTACTGAAAATGAACGGGCTGCGCAGt                                                            >  1:2525224/1‑100 (MQ=255)

                                      aaTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTg                                                          <  1:290967/100‑1 (MQ=255)

                                      aaTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTg                                                          <  1:3323289/100‑1 (MQ=255)

                                      aaTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTg                                                          <  2:1980120/100‑1 (MQ=255)

                                       aTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGc                                                         >  1:2758949/1‑100 (MQ=255)

                                       aTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGc                                                         >  2:1858011/1‑100 (MQ=255)

                                       aTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTATTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGc                                                         >  1:2167849/1‑100 (MQ=255)

                                          gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                      >  1:3066942/1‑100 (MQ=255)

                                          gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                      >  2:1496097/1‑100 (MQ=255)

                                          gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                      >  1:377417/1‑100 (MQ=255)

                                          gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                      >  1:2101530/1‑100 (MQ=255)

                                          gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                      >  2:2978165/1‑100 (MQ=255)

                                           aaCGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                      <  2:2919427/99‑1 (MQ=255)

                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATAttn                                                   <  2:908477/100‑2 (MQ=255)

                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTc                                                   <  2:2080079/100‑1 (MQ=255)

                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTc                                                   <  2:2813258/100‑1 (MQ=255)

                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTc                                                   >  2:3159613/1‑100 (MQ=255)

                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTc                                                   >  1:1241577/1‑100 (MQ=255)

                                               cTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAg                                                 >  2:1388309/1‑100 (MQ=255)

                                               cTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAg                                                 >  2:2646447/1‑100 (MQ=255)

                                                ttCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGa                                                <  2:1005654/100‑1 (MQ=255)

                                                 tCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGAt                                               >  2:2160553/1‑100 (MQ=255)

                                                 tCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGAt                                               <  1:3010816/100‑1 (MQ=255)

                                                 tCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCACTTGCATATTCAGAt                                               >  1:2936000/1‑100 (MQ=255)

                                                  cacaGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATa                                              >  2:446916/1‑100 (MQ=255)

                                                    caGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATaa                                             >  1:3214887/1‑99 (MQ=255)

                                                    caGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAAc                                            <  2:1159977/100‑1 (MQ=255)

                                                    caGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAAc                                            >  2:2425555/1‑100 (MQ=255)

                                                     aGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAAcc                                           <  2:3206383/100‑1 (MQ=255)

                                                     aGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAAcc                                           <  2:2406785/100‑1 (MQ=255)

                                                      gCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCg                                          >  1:859327/1‑100 (MQ=255)

                                                       cACTTTTCGACGCGCATCATATGAACCTTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGACCATGAACCGGCTGCGCAGTTGCATATTCAGATAAcc                                           >  1:1530572/1‑98 (MQ=255)

                                                         cTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGccc                                       >  2:2531266/1‑100 (MQ=255)

                                                          ttttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGccc                                       <  1:809556/99‑1 (MQ=255)

                                                          ttttCGACGCGCATCATATGAACATTGCGGAGCTGGCGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCca                                      <  2:2380223/100‑1 (MQ=255)

                                                           tttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAATATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCcac                                     <  1:1841896/100‑1 (MQ=255)

                                                           tttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCcac                                     <  2:1836056/100‑1 (MQ=255)

                                                           tttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCcac                                     <  1:328529/100‑1 (MQ=255)

                                                           tttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCcac                                     <  1:3338702/100‑1 (MQ=255)

                                                            ttCGAAGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCcaca                                    <  1:1183966/100‑1 (MQ=255)

                                                             tCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAg                                   <  2:2237202/100‑1 (MQ=255)

                                                              cGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGc                                  <  2:596086/100‑1 (MQ=255)

                                                                 cgcgCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGcccc                               <  1:3203357/100‑1 (MQ=255)

                                                                  gcgcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCg                              <  1:2305120/100‑1 (MQ=255)

                                                                  gcgcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCg                              <  2:1479604/100‑1 (MQ=255)

                                                                   cgcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGc                             >  1:517826/1‑100 (MQ=255)

                                                                    gcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAACTGAACGGGCTGCGCAGTTGAATATTCAGATAACCGCCCACAGCCCCGCa                            >  1:792269/1‑100 (MQ=255)

                                                                    gcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCa                            <  1:750073/100‑1 (MQ=255)

                                                                       tcatATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCt                         >  2:779646/1‑100 (MQ=255)

                                                                          tatGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCg                      <  2:562742/100‑1 (MQ=255)

                                                                           atGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCgg                     <  2:3254188/100‑1 (MQ=255)

                                                                            tGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGa                    <  2:3060468/100‑1 (MQ=255)

                                                                            tGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGa                    <  1:2425537/100‑1 (MQ=255)

                                                                             gAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGAc                   >  1:2480217/1‑100 (MQ=255)

                                                                                  ttGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgcagc              <  2:267268/100‑1 (MQ=255)

                                                                                  ttGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgcagc              <  2:543239/100‑1 (MQ=255)

                                                                                   tGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgcagca             <  2:1618530/100‑1 (MQ=255)

                                                                                    gCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCaa            <  2:1512963/100‑1 (MQ=255)

                                                                                        aGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAatat        <  2:1865241/100‑1 (MQ=255)

                                                                                          cTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTg      <  2:2582059/100‑1 (MQ=255)

                                                                                          cTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTg      <  1:1590139/100‑1 (MQ=255)

                                                                                          cTGGTGTTGCGCACGCAACATGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTg      <  1:308519/100‑1 (MQ=255)

                                                                                           tGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGACAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGa     >  1:2372521/1‑100 (MQ=255)

                                                                                            ggTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAg    <  1:1401766/100‑1 (MQ=255)

                                                                                            ggTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAg    >  2:1810513/1‑100 (MQ=255)

                                                                                             gtgtTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGc   <  2:290285/100‑1 (MQ=255)

                                                                                             gtgtTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGc   >  2:1354251/1‑100 (MQ=255)

                                                                                              tgtTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGCa  >  2:357283/1‑100 (MQ=255)

GTCTGGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGCA  >  NC_000913/2600191‑2600384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`