Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,638,037 C→A 67.6% A206S (GCG→TCG)  bamB ← BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,638,0370CA67.6% 142.2 / 111.9 114A206S (GCG→TCG) bamBBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (14/23);  new base A (36/41);  total (50/64)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

AACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCG  >  NC_000913/2637941‑2638135
                                                                                                |                                                                                                  
 aCCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCa                                                                                                <  2:2205453/100‑1 (MQ=255)
  ccGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCaa                                                                                               >  1:299310/1‑100 (MQ=255)
   cGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCaaa                                                                                              <  1:3355064/100‑1 (MQ=255)
    ggTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCaaaa                                                                                             >  1:1697303/1‑100 (MQ=255)
     gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCGCCAAAAg                                                                                            <  2:1769991/100‑1 (MQ=255)
       cGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGcc                                                                                          >  2:2823272/1‑100 (MQ=255)
         ccTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGt                                                                                        >  2:3107911/1‑100 (MQ=255)
         ccTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGt                                                                                        >  1:1520000/1‑100 (MQ=255)
          cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGtt                                                                                       <  2:1031522/100‑1 (MQ=255)
          cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGtt                                                                                       <  1:2401375/100‑1 (MQ=255)
          cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGtt                                                                                       >  2:2272738/1‑100 (MQ=255)
           tgtgAAGTACGCTGGTGCCCAATCATCTGGCCCTGTTCCCTCAGCACTGCGCTGCCGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGt                                                                                        <  1:2952425/95‑1 (MQ=255)
           tGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTg                                                                                      <  2:725602/100‑1 (MQ=255)
            gggAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTg                                                                                      >  1:1674014/1‑99 (MQ=255)
               aaaTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCgg                                                                                  <  1:3017430/100‑1 (MQ=255)
               aaaTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCgg                                                                                  <  2:2265389/100‑1 (MQ=255)
               aaaTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCgg                                                                                  <  2:2450499/100‑1 (MQ=255)
               aaaTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCgg                                                                                  <  2:1050510/100‑1 (MQ=255)
                 atgcGCTGCTGTCAAATGATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgcg                                                                                <  1:2085502/97‑1 (MQ=255)
                 aTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgcg                                                                                <  2:3318123/100‑1 (MQ=255)
                 aTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgcg                                                                                <  1:2731427/100‑1 (MQ=255)
                  tACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgcgc                                                                               <  2:814201/100‑1 (MQ=255)
                   aCGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCa                                                                              <  2:1697312/100‑1 (MQ=255)
                    cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTTTCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAg                                                                             <  2:1882931/100‑1 (MQ=255)
                    cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAg                                                                             <  2:478632/100‑1 (MQ=255)
                      ctgctgCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGAc                                                                           <  1:1004358/100‑1 (MQ=255)
                      ctgctgCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGAc                                                                           <  1:3025949/100‑1 (MQ=255)
                          tgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGCCCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTTTCGGCGCAGACTCGc                                                                       >  2:2845029/1‑100 (MQ=255)
                           gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                      <  2:2768114/100‑1 (MQ=255)
                           gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                      >  1:999065/1‑100 (MQ=255)
                           gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAACCCGTTGTCGGCGCTGACTCGcc                                                                      >  1:1536086/1‑100 (MQ=255)
                            ccAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCa                                                                     >  2:2138674/1‑100 (MQ=255)
                             cAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCAc                                                                    >  2:2264522/1‑100 (MQ=255)
                              aaaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACg                                                                   >  2:1433953/1‑100 (MQ=255)
                                    tcTGGCCCTGTTCCATCAGCACTGCGCTGACGCTACCATTATCGCCCCCCACGACGGCCGAACCAAATGCCGTTGTCGGCGCAGACTCGCCACGCAAaga                                                             <  2:544705/100‑1 (MQ=255)
                                     cTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagag                                                            >  1:835784/1‑100 (MQ=255)
                                      tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagaga                                                           >  2:1183871/1‑100 (MQ=255)
                                       ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagagag                                                          <  2:3003539/100‑1 (MQ=255)
                                       ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagagag                                                          <  1:3012811/100‑1 (MQ=255)
                                        gCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGc                                                         <  2:1381890/100‑1 (MQ=255)
                                        gCCCTGCCCCAGCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagagag                                                          <  1:2798859/99‑1 (MQ=255)
                                          ccTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCTCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGa                                                       <  1:2371948/100‑1 (MQ=255)
                                          ccTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGa                                                       >  1:1534870/1‑100 (MQ=255)
                                           cTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                     <  2:1723476/100‑1 (MQ=255)
                                            tGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                     <  2:1281582/100‑1 (MQ=255)
                                              ttCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGc                                                   <  1:378158/100‑1 (MQ=255)
                                              ttCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGc                                                   <  2:2688214/100‑1 (MQ=255)
                                               tCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                                  >  2:2554464/1‑100 (MQ=255)
                                               tCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                                  >  2:2666060/1‑100 (MQ=255)
                                                ccATCAGCACTGCGCTGACGCGGCCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                                  <  1:2125652/99‑1 (MQ=255)
                                                 cATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTTGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                >  2:3157841/1‑100 (MQ=255)
                                                 cATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                >  2:2673436/1‑100 (MQ=255)
                                                 cATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGACCGAAGGCata                                                >  1:1216742/1‑100 (MQ=255)
                                                   tCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATc                                              >  1:710998/1‑100 (MQ=255)
                                                    cAGCACTGCGCTGACGCGACCATTATTGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGTCa                                                  >  1:259418/1‑95 (MQ=255)
                                                     aGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGTCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGa                                            <  2:1502798/100‑1 (MQ=255)
                                                      gCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAg                                           <  2:2673372/100‑1 (MQ=255)
                                                      gCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAg                                           >  1:3130464/1‑100 (MQ=255)
                                                        aCTGCGCTGACGCGACCATTATCTCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGt                                         <  1:1036769/100‑1 (MQ=255)
                                                        aCTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGt                                         >  2:1394850/1‑100 (MQ=255)
                                                         cgccgcTGACGCGATCATTATCGCCCCCCACGACGGCCGCTCCAAAAGCCGTTGTCCGCGCAGAGTCGCCACGCAAAGAGAGCGAACGCATATCGAg                                           <  1:3061460/94‑1 (MQ=255)
                                                            cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                     <  1:1824129/100‑1 (MQ=255)
                                                            cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                     <  2:1505317/100‑1 (MQ=255)
                                                            cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                     <  2:635164/100‑1 (MQ=255)
                                                             gcTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACt                                    <  1:64476/100‑1 (MQ=255)
                                                             gcTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACt                                    >  2:1921549/1‑100 (MQ=255)
                                                              cTGACGCGCCCATTATCGCCCCCCCCGACGGCCGAACACACAGCCGTTGTCGGCGCAGGCTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                   <  1:1843950/100‑1 (MQ=255)
                                                              cTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                   <  1:2554444/100‑1 (MQ=255)
                                                                gACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTc                                 >  1:1678364/1‑100 (MQ=255)
                                                                gACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTc                                 >  1:2701191/1‑100 (MQ=255)
                                                                gACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTc                                 >  1:3364200/1‑100 (MQ=255)
                                                                gACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTc                                 <  2:374259/100‑1 (MQ=255)
                                                                  cgcgACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCa                               >  2:186560/1‑100 (MQ=255)
                                                                  cgcgACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCa                               >  2:3257695/1‑100 (MQ=255)
                                                                     gACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCAttt                            >  1:83727/1‑100 (MQ=255)
                                                                      aCCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                           <  2:2462331/100‑1 (MQ=255)
                                                                      aCCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                           <  2:137859/100‑1 (MQ=255)
                                                                      aCCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                           <  1:1504973/100‑1 (MQ=255)
                                                                      aCCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                           >  2:149635/1‑100 (MQ=255)
                                                                        cATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGAc                         <  1:1672282/100‑1 (MQ=255)
                                                                        cATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGAc                         <  1:2272722/100‑1 (MQ=255)
                                                                            agcGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCGGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcg                     <  1:2887316/98‑1 (MQ=255)
                                                                            aTCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcg                     >  1:3148585/1‑100 (MQ=255)
                                                                            aTCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcg                     <  2:3364228/100‑1 (MQ=255)
                                                                            aTCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcg                     >  2:1896433/1‑100 (MQ=255)
                                                                            aTCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCAATTTGACAgc                      >  1:2972247/1‑99 (MQ=255)
                                                                             tCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcgc                    <  1:3175778/100‑1 (MQ=255)
                                                                             tCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcgc                    >  2:415308/1‑100 (MQ=255)
                                                                             tCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCCAAGGCATATCGAGGTTAACTGTCCATTTGACAgcgc                    <  2:2389486/100‑1 (MQ=255)
                                                                              cGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGcc                   <  2:2205932/100‑1 (MQ=255)
                                                                               gCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTACATTTGACAGCGCCg                  >  2:3332176/1‑100 (MQ=255)
                                                                               gCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCg                  >  1:1103992/1‑100 (MQ=255)
                                                                                  ccccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCa               >  2:1614571/1‑100 (MQ=255)
                                                                                   cccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAg              >  2:3320841/1‑100 (MQ=255)
                                                                                    ccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc             <  1:1736682/100‑1 (MQ=255)
                                                                                        gacgGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCg         <  1:824865/100‑1 (MQ=255)
                                                                                          cgGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGtt       <  2:3323862/100‑1 (MQ=255)
                                                                                           ggCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTc      <  1:2856660/100‑1 (MQ=255)
                                                                                             ccGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAg    >  2:3265674/1‑100 (MQ=255)
                                                                                             ccGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAg    >  2:286337/1‑100 (MQ=255)
                                                                                                |                                                                                                  
AACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCG  >  NC_000913/2637941‑2638135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑