Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,712,402 A→G 21.3% S121S (AGT→AGC trmN ← tRNA1(Val) (adenine(37)‑N6)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,712,4020AG21.3% 265.1 / 74.4 141S121S (AGT→AGCtrmNtRNA1(Val) (adenine(37)‑N6)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (61/50);  new base G (16/14);  total (77/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 145 total aligned reads displayed.

CAGGTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGATA  >  NC_000913/2712304‑2712501
                                                                                                  |                                                                                                   
caGGTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGCTTAc                                                                                                    >  1:1032550/1‑100 (MQ=255)
 aGGTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCt                                                                                                   <  1:2668614/100‑1 (MQ=255)
  ggTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtg                                                                                                  >  1:2829080/1‑100 (MQ=255)
  ggTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtg                                                                                                  <  1:3161573/100‑1 (MQ=255)
  ggTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtg                                                                                                  >  2:1284496/1‑100 (MQ=255)
  ggTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACt                                                                                                   >  1:1592406/1‑99 (MQ=255)
   gTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtga                                                                                                 <  1:346825/100‑1 (MQ=255)
   gTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtg                                                                                                  >  1:1934644/1‑99 (MQ=255)
    tctgCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtgat                                                                                                <  2:2306312/97‑1 (MQ=255)
     cAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtgatg                                                                                               >  2:1250800/1‑100 (MQ=255)
     cAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtgatg                                                                                               >  1:1004548/1‑100 (MQ=255)
      aGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtgatga                                                                                              >  1:3313444/1‑100 (MQ=255)
      aGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtgatga                                                                                              <  1:2456289/100‑1 (MQ=255)
      aGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtgatga                                                                                              >  1:732052/1‑100 (MQ=255)
       gCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTgatgat                                                                                             <  1:3092729/100‑1 (MQ=255)
       gCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtgataga                                                                                             >  1:2306296/1‑97 (MQ=255)
        cAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACtgatg                                                                                               >  2:1068462/1‑97 (MQ=255)
        cAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGAtt                                                                                            >  2:1186884/1‑100 (MQ=255)
         aaCGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTa                                                                                           <  2:3162713/100‑1 (MQ=255)
         aaCGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTa                                                                                           <  1:2508481/100‑1 (MQ=255)
         aaCGAGTGGTGATTAAGCGGAGGGGTATAGCGAGCCTGTTGCCAGTGGGGTGTTGTGGACTATACTCCCTGCGGATAGTAAGGTGGGGTGCTgatgat                                                                                             <  1:2037877/98‑1 (MQ=255)
           cGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTaaa                                                                                         <  2:2573191/100‑1 (MQ=255)
              tGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCg                                                                                      >  2:1640070/1‑100 (MQ=255)
               gggTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGa                                                                                     <  2:2834063/100‑1 (MQ=255)
               gggTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGa                                                                                     <  1:2841084/100‑1 (MQ=255)
                ggTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTACGGTGGGTTACTGATGATTAAATCgca                                                                                    >  1:3205831/1‑98 (MQ=255)
                ggTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGaa                                                                                    >  2:3025403/1‑100 (MQ=255)
                   gATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCg                                                                                 <  1:2095024/100‑1 (MQ=255)
                   gATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCg                                                                                 >  1:2635231/1‑100 (MQ=255)
                     tCAAGCTTAGAGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGGAACGTGGGTTACTGATGATTAAATCGAAGCGTa                                                                               <  1:2226924/100‑1 (MQ=255)
                     tCAAGCGTAGTGGACTAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGGTAGTAAGGCGGGTTACTGATGCTTAAATCGAAGCg                                                                                 >  1:1099116/1‑98 (MQ=255)
                      cAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTAc                                                                              <  1:1418868/100‑1 (MQ=255)
                        aGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTACTg                                                                            <  2:539238/100‑1 (MQ=255)
                            tAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTACTGTctg                                                                        >  2:144343/1‑100 (MQ=255)
                              gTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTACTGTctgct                                                                      <  1:2866827/100‑1 (MQ=255)
                              gTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTctgct                                                                      >  2:1978454/1‑100 (MQ=255)
                              gTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTctgct                                                                      <  2:2166270/100‑1 (MQ=255)
                               tGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTctgctg                                                                     <  2:2829103/100‑1 (MQ=255)
                                ggTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCtgt                                                                    >  1:1198780/1‑100 (MQ=255)
                                ggTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCtgt                                                                    >  1:801160/1‑100 (MQ=255)
                                  tataGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCtgtgt                                                                  <  2:458464/100‑1 (MQ=255)
                                   ataGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCtgtgtg                                                                 >  2:367411/1‑100 (MQ=255)
                                     aGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTCCTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTAATGTCTGCtgtgtg                                                                 <  1:271509/98‑1 (MQ=255)
                                      gCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATc                                                              <  1:1427015/100‑1 (MQ=255)
                                      gCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATc                                                              >  1:3246582/1‑100 (MQ=255)
                                       cGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATcc                                                             >  1:1930530/1‑100 (MQ=255)
                                         aGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCAc                                                           >  2:318210/1‑100 (MQ=255)
                                            cTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCActgc                                                        >  2:134248/1‑100 (MQ=255)
                                            cTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCActgc                                                        <  2:1191979/100‑1 (MQ=255)
                                              gTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCActgctg                                                      <  1:2007041/100‑1 (MQ=255)
                                              gTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCActgctg                                                      >  2:560099/1‑100 (MQ=255)
                                              gTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCActgctg                                                      <  1:1701705/100‑1 (MQ=255)
                                                tCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGaa                                                    <  2:1613043/100‑1 (MQ=255)
                                                 cccGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAat                                                   <  1:2621679/100‑1 (MQ=255)
                                                 cccGTGGAGGGGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAat                                                   <  1:2209574/100‑1 (MQ=255)
                                                  ccGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAata                                                  <  2:3351203/100‑1 (MQ=255)
                                                     ttGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATcc                                               <  2:2982068/100‑1 (MQ=255)
                                                      tGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAATGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATccg                                              <  1:1155509/100‑1 (MQ=255)
                                                      tGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATccg                                              <  2:708755/100‑1 (MQ=255)
                                                      tGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACCGTCTGCTGTGTGATCCATTGCTGAATATccg                                              <  2:2282952/100‑1 (MQ=255)
                                                       gAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATAAccgc                                             <  1:318209/100‑1 (MQ=255)
                                                          gtgtCGCGCACTCTACTCCCGGCTGATAGTAAGGTGGGTTGCTGATGAGTAAATCGAAGCGTACTGTCTGCTGTGTGATCGACTGCAGAATATccgc                                             >  2:72364/1‑97 (MQ=255)
                                                          gtgtCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGt                                          >  2:1108555/1‑100 (MQ=255)
                                                          gtgtCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGt                                          >  1:3078206/1‑100 (MQ=255)
                                                          gtgtCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGt                                          >  2:2508995/1‑100 (MQ=255)
                                                           tgtCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTa                                         >  1:185519/1‑100 (MQ=255)
                                                           tgtCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTa                                         <  1:388984/100‑1 (MQ=255)
                                                            gtCGAGCGCTCGACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACGGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTAt                                        <  1:1787690/100‑1 (MQ=255)
                                                            gtCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTAt                                        <  1:1597463/100‑1 (MQ=255)
                                                             tCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATg                                       <  2:1705039/100‑1 (MQ=255)
                                                              cGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATgg                                      >  1:2698734/1‑100 (MQ=255)
                                                              cGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATgg                                      >  2:3115862/1‑100 (MQ=255)
                                                                 gCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGAc                                    <  1:872177/99‑1 (MQ=255)
                                                                   aCTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGtt                                 <  1:897789/100‑1 (MQ=255)
                                                                    ctctACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGCTTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGATGAATATCCGCCGTATGGACGtt                                 <  1:804733/99‑1 (MQ=255)
                                                                    ctctACTCCCTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTa                                >  2:2786300/1‑100 (MQ=255)
                                                                      ctACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAAt                              >  2:3277913/1‑100 (MQ=255)
                                                                      ctACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAAt                              >  2:2242637/1‑100 (MQ=255)
                                                                           cccTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGct                         >  1:1356926/1‑100 (MQ=255)
                                                                            ccTGCTGATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGctc                        >  2:180791/1‑100 (MQ=255)
                                                                                 tgATAGTAAGGTGGGTTGCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGccc                   <  2:932014/100‑1 (MQ=255)
                                                                                  gATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCa                  >  1:379170/1‑100 (MQ=255)
                                                                                     aGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACgg               <  2:2663892/100‑1 (MQ=255)
                                                                                     aGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACgg               >  1:1658440/1‑100 (MQ=255)
                                                                                      gTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACggg              >  2:1554170/1‑100 (MQ=255)
                                                                                       tAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACgggg             >  2:2975900/1‑100 (MQ=255)
                                                                                       tAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACgggg             >  1:1614788/1‑100 (MQ=255)
                                                                                       tAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACgggg             <  1:2177407/100‑1 (MQ=255)
                                                                                       tAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACggg              >  2:2010754/1‑99 (MQ=255)
                                                                                          ggTGGGTTGCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACt          >  1:580771/1‑100 (MQ=255)
                                                                                           gTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTg         >  1:558234/1‑100 (MQ=255)
                                                                                            tGGGTTGCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTgg        <  2:2077501/100‑1 (MQ=255)
                                                                                            tGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTgg        <  1:2242621/100‑1 (MQ=255)
                                                                                             gggTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTg         >  1:1452855/1‑98 (MQ=255)
                                                                                              ggTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGtt      <  2:964903/100‑1 (MQ=255)
                                                                                               gTTGCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTg     >  2:3316123/1‑100 (MQ=255)
                                                                                               gTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTg     >  1:3349400/1‑100 (MQ=255)
                                                                                                  aCTGATGATTACATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGat   <  1:2875088/99‑1 (MQ=255)
                                                                                                  aCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGata  <  2:2646325/100‑1 (MQ=255)
                                                                                                  aCTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGata  >  1:2314182/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CAGGTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGATAGTAAGGTGGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGATA  >  NC_000913/2712304‑2712501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: