Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,782,883 G→A 25.3% intergenic (‑157/+182) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,782,8830GA25.3% 284.8 / 99.9 160intergenic (‑157/+182)ypjA/pinHadhesin‑like autotransporter/pseudogene, invertase resolvase family
Reads supporting (aligned to +/- strand):  ref base G (69/49);  new base A (20/20);  total (91/69)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

Only 100 of 175 total aligned reads displayed.

TGCAACATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTC  >  NC_000913/2782784‑2782981
                                                                                                   |                                                                                                  
   aaCATCAGCCCACAGGCACCAGACCAGGTGATTCATCCTGAAGCGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAatgatg                                                                                                 <  1:1282869/100‑1 (MQ=255)
    aCATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAAtgatga                                                                                                <  1:736472/100‑1 (MQ=255)
    aCATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAAtaatga                                                                                                >  1:1694419/1‑100 (MQ=255)
    aCATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAAtaatga                                                                                                <  2:3298414/100‑1 (MQ=255)
     cATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGTCAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATgatgat                                                                                               >  1:740159/1‑100 (MQ=255)
     cATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATgatgat                                                                                               >  2:313622/1‑100 (MQ=255)
     cATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATgatgat                                                                                               <  2:1012823/100‑1 (MQ=255)
     cATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATgatgat                                                                                               >  1:2550529/1‑100 (MQ=40)
       tCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGatat                                                                                             >  2:2895158/1‑100 (MQ=255)
          gCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTcc                                                                                          >  2:571617/1‑100 (MQ=255)
            ccACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGc                                                                                        >  2:1209832/1‑100 (MQ=255)
             cacaGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCa                                                                                       <  2:1042117/100‑1 (MQ=255)
             cacaGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCa                                                                                       >  1:364352/1‑100 (MQ=255)
             cacaGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCa                                                                                       >  1:3303477/1‑100 (MQ=255)
                aGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATaa                                                                                    <  2:1195939/100‑1 (MQ=255)
                 ggCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATAAAAGACATCATGATGAAATTATGATATTCCGCATAAg                                                                                   >  1:3101235/1‑100 (MQ=255)
                   cACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGaa                                                                                 >  2:1987950/1‑100 (MQ=255)
                     ccagaccggGGCATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGCTGAAATAATGATATTCCGCATAAGa                                                                                  >  1:1780017/1‑97 (MQ=14)
                     ccagaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATg                                                                               >  2:2689784/1‑100 (MQ=255)
                       agaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAg                                                                             <  1:1921760/100‑1 (MQ=255)
                             ggggATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCCAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCAtt                                                                        >  1:2969609/1‑99 (MQ=255)
                             ggggATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCAttt                                                                       >  2:2069696/1‑100 (MQ=255)
                               ggATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCAttttt                                                                     >  1:2628280/1‑100 (MQ=255)
                               ggATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCAttttt                                                                     <  2:142034/100‑1 (MQ=255)
                               ggATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGAATAAGAATGAGGCAttt                                                                       >  2:623649/1‑98 (MQ=255)
                                gATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCAtttttt                                                                    <  2:1070300/100‑1 (MQ=255)
                                   tCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTaan                                                                 <  2:1601061/100‑2 (MQ=255)
                                   tCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTaaa                                                                 <  1:2601530/100‑1 (MQ=255)
                                    cATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAAc                                                                <  2:778692/100‑1 (MQ=255)
                                    cATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAAc                                                                >  1:2786037/1‑100 (MQ=255)
                                    cATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAAc                                                                <  2:3074661/100‑1 (MQ=255)
                                       ccTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCa                                                             >  2:147253/1‑100 (MQ=255)
                                        cTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAg                                                            <  2:1631139/100‑1 (MQ=255)
                                        cTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAg                                                            <  2:2142683/100‑1 (MQ=255)
                                         tGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCa                                                             >  1:3005270/1‑98 (MQ=255)
                                          gAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTg                                                          >  2:568024/1‑100 (MQ=255)
                                               gaCAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTg                                                     <  2:2601460/100‑1 (MQ=255)
                                               gaCAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTg                                                     >  2:493980/1‑100 (MQ=255)
                                                 cAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGaa                                                   >  2:2936792/1‑100 (MQ=255)
                                                 cAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGaa                                                   >  2:2461639/1‑100 (MQ=255)
                                                   gcgcAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAg                                                  >  1:2100138/1‑99 (MQ=255)
                                                    cgtaaGTGTATTGTTTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAgtg                                                <  2:661230/97‑1 (MQ=255)
                                                    cgcAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGaacgg                                                >  1:1023303/1‑97 (MQ=255)
                                                    cgcAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAgtg                                                <  1:124259/100‑1 (MQ=255)
                                                     gcAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAgtgt                                               <  1:2938065/100‑1 (MQ=255)
                                                      cAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGtgtg                                              >  1:1267043/1‑100 (MQ=255)
                                                       aaGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTgg                                             <  2:2262760/100‑1 (MQ=255)
                                                       aaGGGTATTGTGTTCACCGCTCATCAAAGACATCAGGATGAAAGGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTgg                                             >  2:2700702/1‑100 (MQ=255)
                                                        aGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGt                                            <  1:493978/100‑1 (MQ=255)
                                                        aGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGt                                            <  1:90963/100‑1 (MQ=255)
                                                          tgtATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTg                                          <  1:1633679/100‑1 (MQ=255)
                                                          tgtATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGGATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTg                                          <  1:1413323/100‑1 (MQ=255)
                                                          tgtATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTg                                          <  2:1664274/100‑1 (MQ=255)
                                                           gtATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTgg                                         >  1:2243029/1‑100 (MQ=255)
                                                            tATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTg                                          <  1:1003289/98‑1 (MQ=255)
                                                            tATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGa                                        <  2:3183794/100‑1 (MQ=255)
                                                            tATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGa                                        <  2:2330972/100‑1 (MQ=255)
                                                             aTTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGAt                                       >  2:3126775/1‑100 (MQ=255)
                                                             aTTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGAt                                       >  2:549523/1‑100 (MQ=255)
                                                              ttGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGGATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATa                                      <  1:1987066/100‑1 (MQ=255)
                                                               tgtgTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATaa                                     >  2:2587000/1‑100 (MQ=255)
                                                                ntgtTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATaaa                                    >  2:3138236/2‑100 (MQ=255)
                                                                gtgtTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATaaa                                    >  2:3270016/1‑100 (MQ=255)
                                                                gtgtTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTTTTTTTTTTTTGCTGAAGTGTGGTTGGATaaa                                    >  1:2435902/1‑100 (MQ=14)
                                                                gtgtTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATaaa                                    >  1:738701/1‑100 (MQ=255)
                                                                  gtTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTGATACGCAGTGCGCTGAAGTGTGGTTGGATaaaaa                                  >  1:1775221/1‑100 (MQ=255)
                                                                    tCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCTCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAg                                <  2:3248071/100‑1 (MQ=255)
                                                                    tCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAg                                <  2:717497/100‑1 (MQ=255)
                                                                    tCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAg                                <  2:947960/100‑1 (MQ=255)
                                                                    ncACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAATTTCTTTGCGCTGAAGTGTGGTTGGATAAAAAAg                                >  1:2673244/2‑100 (MQ=17)
                                                                     cACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGt                               >  2:319354/1‑100 (MQ=255)
                                                                      aCCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTc                              <  2:3322830/100‑1 (MQ=255)
                                                                      aCCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTc                              <  1:387676/100‑1 (MQ=255)
                                                                       ccGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCa                             >  1:430661/1‑100 (MQ=255)
                                                                        cGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCaa                            >  2:3384167/1‑100 (MQ=255)
                                                                        cGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCaa                            >  1:973132/1‑100 (MQ=255)
                                                                        cGCTCATCAAAGACATCATGATGAAATGAAGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCaa                            <  1:1336289/100‑1 (MQ=255)
                                                                           tcatcaAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATcc                         <  1:292385/100‑1 (MQ=255)
                                                                            catcaAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCa                        >  1:1461494/1‑100 (MQ=255)
                                                                            catcaAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCa                        >  1:1518128/1‑100 (MQ=255)
                                                                              tcaAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACTCATTCCGCTGAAGTGCGGTTCGATAAAAAAGTCAATCCaat                      >  1:665975/1‑98 (MQ=17)
                                                                              tcaAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCAtt                      >  2:709294/1‑100 (MQ=255)
                                                                               caAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATCAAAAAGTCAATCGATTc                     >  2:1513106/1‑100 (MQ=255)
                                                                               caAAGACATCATGATGAAATGAGGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGATGAAGTGTGGTTGGATAAAAAAGTCAATCCATTc                     >  1:1512786/1‑100 (MQ=255)
                                                                               caAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTc                     >  2:190788/1‑100 (MQ=255)
                                                                                  aGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAgg                  <  2:431691/100‑1 (MQ=255)
                                                                                  aGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAgg                  <  2:408604/100‑1 (MQ=255)
                                                                                   gACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGa                 >  2:1318239/1‑100 (MQ=255)
                                                                                   gACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGa                 <  1:2736180/100‑1 (MQ=255)
                                                                                     catcatGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAAGCCATTCAGGaaa               <  2:2037754/100‑1 (MQ=255)
                                                                                     catcatGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATATAAAAGTCAATCCATTCAGGaaa               >  2:1667233/1‑100 (MQ=255)
                                                                                     catcatGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGaaa               <  1:214486/100‑1 (MQ=255)
                                                                                      atcatGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAAt              >  1:800144/1‑100 (MQ=255)
                                                                                         atgatgAAATGATGATATTCCGCATAAGAATGAGGCATTTCTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACg           >  2:2752432/1‑100 (MQ=255)
                                                                                         atgatgAAATAATGATATTCCGCATAAGAATGATGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGGTAAAAAAGTCAATCCATTCAGGAAATACg           >  1:2042296/1‑100 (MQ=255)
                                                                                           gatgaAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACggg         <  1:2321739/100‑1 (MQ=255)
                                                                                            atgaAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCATTCCATTCAGGAAATACGGGc        <  1:2062715/100‑1 (MQ=255)
                                                                                             tgaAATGATGATATTGCGCATAAGAATGAGGCATTTTTTAAAAGCAGTGCGCTGAGGGGAGAGTGGATAAAAAAGTCAATCAATTCAGGAAATaag           >  1:3039231/1‑94 (MQ=21)
                                                                                             tgaAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCg       <  1:3190269/100‑1 (MQ=255)
                                                                                              gaAATGATGATATTCCGCATAAGAATGAGGCATGTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGt      >  1:2036700/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TGCAACATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTC  >  NC_000913/2782784‑2782981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: