Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,756 G→A 100% intergenic (+77/‑14) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7560GA98.6% 472.5 / ‑3.1 142intergenic (+77/‑14)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (75/65);  total (76/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 143 total aligned reads displayed.

AAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCT  >  NC_000913/2810659‑2810852
                                                                                                 |                                                                                                
aaaGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAg                                                                                                <  2:477543/100‑1 (MQ=255)
 aaGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAgg                                                                                               <  2:1433647/100‑1 (MQ=255)
 aaGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACAATTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAg                                                                                                >  1:1212389/1‑99 (MQ=255)
  aGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGt                                                                                              <  1:3052654/100‑1 (MQ=255)
    tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCa                                                                                            >  1:588991/1‑100 (MQ=255)
     gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCAt                                                                                           >  1:92146/1‑100 (MQ=255)
      aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATa                                                                                          >  2:2901604/1‑100 (MQ=255)
       tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAc                                                                                         >  1:2554945/1‑100 (MQ=255)
         gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAccc                                                                                       >  2:1443352/1‑100 (MQ=255)
          ccgatctataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                      <  2:2801903/94‑1 (MQ=255)
          cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                      >  1:1415645/1‑100 (MQ=255)
          cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTAACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCa                                                                                      >  1:16885/1‑100 (MQ=255)
            aTTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCaaa                                                                                    <  2:329376/100‑1 (MQ=255)
             ttATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAAt                                                                                   >  2:1473353/1‑100 (MQ=255)
              tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATg                                                                                  <  2:1745181/100‑1 (MQ=255)
                 ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCaagatcgg                                                                               >  1:2801880/1‑94 (MQ=255)
                 ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGAt                                                                               <  1:1039196/100‑1 (MQ=255)
                   aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAg                                                                             <  1:1503045/100‑1 (MQ=255)
                    aCCCACAAGAATCATTTTTTTTTTTTTTTTCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAATTTAATAAAGTCATACCCAAATGGAt                                                                               >  1:514378/1‑97 (MQ=255)
                      ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTc                                                                          <  1:2601543/100‑1 (MQ=255)
                       cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                         <  2:2110266/100‑1 (MQ=255)
                       cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                         >  2:1178178/1‑100 (MQ=255)
                       cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                         >  2:3229296/1‑100 (MQ=255)
                         caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGtt                                                                       <  2:3128152/100‑1 (MQ=255)
                          aaGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGttt                                                                      <  1:1899307/100‑1 (MQ=255)
                          aaGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGttt                                                                      <  1:192582/100‑1 (MQ=255)
                          aaGAATCATTTTTCTAAAACAATACAGTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGttt                                                                      >  2:257770/1‑100 (MQ=255)
                            gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTAc                                                                    <  1:2249980/100‑1 (MQ=255)
                              aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGc                                                                  <  1:1320341/100‑1 (MQ=255)
                               tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGcc                                                                 <  1:1637274/100‑1 (MQ=255)
                               tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGc                                                                  >  1:143174/1‑99 (MQ=255)
                                cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGCAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTa                                                                     >  1:1825730/1‑95 (MQ=255)
                                 aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                               >  1:692378/1‑100 (MQ=255)
                                 aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                               <  2:2877115/100‑1 (MQ=255)
                                    tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                            <  1:162719/100‑1 (MQ=255)
                                    tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                            <  2:2613044/100‑1 (MQ=255)
                                     ttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGa                                                           <  2:1949880/100‑1 (MQ=255)
                                       cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCGTACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                         >  2:655296/1‑100 (MQ=255)
                                       cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                         <  2:1329250/100‑1 (MQ=255)
                                       cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                         >  1:1932387/1‑100 (MQ=255)
                                       cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                         >  1:309521/1‑100 (MQ=255)
                                        tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGCAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                        >  1:750883/1‑100 (MQ=255)
                                        tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                        <  1:1233011/100‑1 (MQ=255)
                                        tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                        <  1:1043057/100‑1 (MQ=255)
                                          aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                      <  1:2318259/100‑1 (MQ=255)
                                          aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                      >  1:711265/1‑100 (MQ=255)
                                           aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                     <  1:2405312/100‑1 (MQ=255)
                                           aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                     <  1:1751020/100‑1 (MQ=255)
                                            aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                    <  2:2698850/100‑1 (MQ=255)
                                            aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                    <  1:2044223/100‑1 (MQ=255)
                                            aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                    >  1:1785060/1‑100 (MQ=255)
                                             cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                   <  1:142270/100‑1 (MQ=255)
                                              aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCTGCTGAAATTAATAAGGTCACACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                  <  1:1848879/100‑1 (MQ=255)
                                                 aCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                               <  2:2554965/100‑1 (MQ=255)
                                                  cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                              >  1:388876/1‑100 (MQ=255)
                                                  cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                              >  2:3131768/1‑100 (MQ=255)
                                                  cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                              >  1:1721193/1‑100 (MQ=255)
                                                  cATTTACTTTATTTGTCACTGTCGTTACTATATCAGCTGAAATTAATAAAGTAATACACAAATGGATAGTAAGTTTACGACCATTGAACAAATAGTaaaa                                              >  1:2664889/1‑100 (MQ=255)
                                                    tttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                            <  2:21492/100‑1 (MQ=255)
                                                    tttACTTTATTTGTCACTGTCGTTACTATATCGGATGAAATTAATAAGGTCATACACAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                            >  1:1093606/1‑100 (MQ=255)
                                                     ttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAttt                                           <  2:2634119/100‑1 (MQ=255)
                                                        cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGTCCATTGAACAAATGCTAAAATTTcgc                                        >  1:1024886/1‑100 (MQ=255)
                                                        cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                        >  2:1004072/1‑100 (MQ=255)
                                                        cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                        >  2:1901478/1‑100 (MQ=255)
                                                         tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                       >  1:2135981/1‑100 (MQ=255)
                                                         tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                       <  1:2725865/100‑1 (MQ=255)
                                                          ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                      <  2:2537550/100‑1 (MQ=255)
                                                            aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCa                                    >  2:2419102/1‑100 (MQ=255)
                                                             tttGTCACTGTCGTTACTATATCGGCTGACATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                   <  1:2042954/100‑1 (MQ=255)
                                                             tttGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                   >  2:1180017/1‑100 (MQ=255)
                                                                gTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                <  2:1068163/100‑1 (MQ=255)
                                                                gTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                <  1:2134914/100‑1 (MQ=255)
                                                                  cACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                              >  2:1277203/1‑100 (MQ=255)
                                                                    cTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCAc                            <  1:11042/100‑1 (MQ=255)
                                                                     tGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                           >  2:2600142/1‑100 (MQ=255)
                                                                     tGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                           <  2:426871/100‑1 (MQ=255)
                                                                      gTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACCAATGCTAAAATTTCGCGCCAGCCGCCACGa                          >  2:3008385/1‑100 (MQ=255)
                                                                        cGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                        >  2:791694/1‑100 (MQ=255)
                                                                        cGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                        <  1:1898853/100‑1 (MQ=255)
                                                                        cGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                        >  2:1689732/1‑100 (MQ=255)
                                                                        cGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                        >  2:1935309/1‑100 (MQ=255)
                                                                         gTTACTATATCGGCTGAAATTAATAATGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                 <  1:810026/90‑1 (MQ=255)
                                                                         gTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                       <  1:534125/100‑1 (MQ=255)
                                                                            aCTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                    >  1:2096323/1‑100 (MQ=255)
                                                                             cTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                   >  1:1976919/1‑100 (MQ=255)
                                                                              tataTCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                  <  1:219241/100‑1 (MQ=255)
                                                                               atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 >  2:2422231/1‑100 (MQ=255)
                                                                               atatCGGCTGAAATTAATAAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                 <  2:2049173/100‑1 (MQ=255)
                                                                                tatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                <  2:3226707/100‑1 (MQ=255)
                                                                                tatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                >  1:2388674/1‑100 (MQ=255)
                                                                                 atCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt               >  1:129356/1‑100 (MQ=255)
                                                                                  tCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTa              <  2:596594/100‑1 (MQ=255)
                                                                                   cGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt             >  1:1151374/1‑100 (MQ=255)
                                                                                     gCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAGGATTTTCCTTATCa           >  1:2310918/1‑100 (MQ=255)
                                                                                      cTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg          >  2:2980559/1‑100 (MQ=255)
                                                                                      cTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg          >  2:192805/1‑100 (MQ=255)
                                                                                         aaaTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga        >  2:849354/1‑99 (MQ=255)
                                                                                          aaTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga      >  1:247277/1‑100 (MQ=255)
                                                                                          aaTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCAAGCCGCCACGAAGATTTTCCTTATCAGgaga      >  1:594631/1‑100 (MQ=255)
                                                                                              aaTAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt  >  1:1977705/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCT  >  NC_000913/2810659‑2810852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: