Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,291,109 A→T 16.7% K766* (AAA→TAA) ‡ yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,1090AT16.7% 332.4 / 53.8 132K766* (AAA→TAA) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (58/52);  new base T (12/10);  total (70/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-01

Only 100 of 134 total aligned reads displayed.

ACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGT  >  NC_000913/3291010‑3291207
                                                                                                   |                                                                                                  
 cacaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                   <  1:507786/100‑1 (MQ=255)
    aGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAAcc                                                                                                <  2:2900755/100‑1 (MQ=255)
       ttGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACTGTAAACCGtt                                                                                             >  1:1037423/1‑100 (MQ=255)
        tgtgGTGCCCAACAGAGGTGCAATGGTAGCGGCGTGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTg                                                                                            <  1:1785304/100‑1 (MQ=255)
          tggtgCCCAACAGAGGTGCAATGGGAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcg                                                                                          <  1:1430777/100‑2 (MQ=255)
           ggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGccc                                                                                         <  1:3353730/100‑1 (MQ=255)
           ggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGccc                                                                                         >  2:3245826/1‑100 (MQ=255)
            gtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgat                                                                                        <  1:214643/100‑4 (MQ=255)
             tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatc                                                                                       <  1:3039433/100‑5 (MQ=255)
             tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatc                                                                                       <  2:1347637/100‑5 (MQ=255)
              gCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatcg                                                                                      <  1:3137308/100‑6 (MQ=255)
              gCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCttt                                                                                      >  2:3230440/1‑100 (MQ=255)
               cccAGCAGAGGTGCAATGGTAGCGGCCCGTTTTACCGCCAATATCGGTTATCCCGTACTTGTTACAGTCAGCGATCGCAACGTTTTTCCGTTGcg                                                                                          <  1:173785/95‑2 (MQ=255)
               cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatcgc                                                                                     <  2:1926782/100‑7 (MQ=255)
                ccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTgg                                                                                    <  2:1514386/100‑1 (MQ=255)
                 cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgc                                                                                   <  2:2740367/100‑1 (MQ=255)
                  aaCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgcg                                                                                  <  2:3153035/100‑1 (MQ=255)
                        ggTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTg                                                                            <  2:1731774/100‑1 (MQ=255)
                        ggTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTg                                                                            >  2:2475462/1‑100 (MQ=255)
                         gTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                           <  2:2898879/100‑1 (MQ=255)
                          tGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGcc                                                                          <  2:2016616/100‑1 (MQ=255)
                           gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCa                                                                         >  2:2755994/1‑100 (MQ=255)
                            cAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAg                                                                        >  1:1076560/1‑100 (MQ=255)
                            cAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAg                                                                        >  1:244646/1‑100 (MQ=255)
                                ggTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                    <  1:1103673/100‑1 (MQ=255)
                                    gcggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGAGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatg                                                                >  1:2229170/1‑100 (MQ=255)
                                      ggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatgat                                                              >  2:2525623/1‑100 (MQ=255)
                                        cgcgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATAc                                                            <  2:2939010/100‑1 (MQ=255)
                                          cgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgg                                                          >  1:101793/1‑100 (MQ=255)
                                           gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACggg                                                         >  1:467920/1‑100 (MQ=255)
                                           gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACggg                                                         <  2:701073/100‑1 (MQ=255)
                                           gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACggg                                                         >  2:3088726/1‑100 (MQ=255)
                                           gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACggg                                                         <  2:3294658/100‑1 (MQ=255)
                                            tttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                        >  1:1221076/1‑100 (MQ=255)
                                            tttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCATTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                        <  1:2504584/100‑1 (MQ=255)
                                             ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                       >  1:802008/1‑100 (MQ=255)
                                             ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                       >  2:2618615/1‑100 (MQ=255)
                                             ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                       >  2:660563/1‑100 (MQ=255)
                                             ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                       >  1:914204/1‑100 (MQ=255)
                                              tCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGca                                                      <  2:1463001/100‑1 (MQ=255)
                                              tCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGca                                                      >  1:306883/1‑100 (MQ=255)
                                                  cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGcaacaa                                                  >  1:2885967/1‑100 (MQ=255)
                                                    ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                <  2:467922/100‑1 (MQ=255)
                                                    ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                <  1:1517475/100‑1 (MQ=255)
                                                    ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                <  2:1057765/100‑1 (MQ=255)
                                                    ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                <  2:3044922/100‑1 (MQ=255)
                                                     cAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGt                                               <  2:53855/100‑1 (MQ=255)
                                                      aaTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCGCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTa                                              <  1:1046383/100‑1 (MQ=255)
                                                      aaTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                 >  1:2674771/1‑97 (MQ=255)
                                                              ttATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGAt                                      <  2:1585506/100‑1 (MQ=255)
                                                               tATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATg                                     >  1:3308349/1‑100 (MQ=255)
                                                                   gcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAggg                                 >  2:1415844/1‑100 (MQ=255)
                                                                     gTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcg                               <  2:2682143/100‑1 (MQ=255)
                                                                      tACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcgg                              >  2:3187197/1‑100 (MQ=255)
                                                                      tACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcgg                              <  2:895641/100‑1 (MQ=255)
                                                                       aCTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcggc                             <  1:2121252/100‑1 (MQ=255)
                                                                        cTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcgaga                            >  1:1336529/1‑97 (MQ=255)
                                                                        cTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCa                            >  2:1012389/1‑100 (MQ=255)
                                                                        cTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTCTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCa                            >  2:2277602/1‑100 (MQ=255)
                                                                          tGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATa                          >  1:3314127/1‑100 (MQ=255)
                                                                          tGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATa                          <  2:613780/100‑1 (MQ=255)
                                                                           gTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATAc                         <  2:1291560/100‑1 (MQ=255)
                                                                             tACAGTCAGCGTTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACTATGGTACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATa                          <  1:769079/97‑1 (MQ=255)
                                                                             tACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                       <  1:1899080/100‑1 (MQ=255)
                                                                              aCAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtat                      >  1:3154160/1‑100 (MQ=255)
                                                                              aCAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtat                      <  2:2713461/100‑1 (MQ=255)
                                                                              aCAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtac                      >  1:536191/1‑99 (MQ=255)
                                                                               naGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata                     >  1:1910544/2‑100 (MQ=255)
                                                                               cAGTCAGCTATCGCAACGGTAAACCTTTCCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATTAGGGCGGCATACtat                      <  1:3075937/99‑1 (MQ=255)
                                                                               cAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGCTACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata                     >  1:2439469/1‑100 (MQ=255)
                                                                                   cAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctc                 <  2:2521111/100‑1 (MQ=255)
                                                                                   cAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctc                 >  2:1220614/1‑100 (MQ=255)
                                                                                    ccaaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctct                >  2:3204949/8‑100 (MQ=255)
                                                                                    aGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctct                >  1:2495275/1‑100 (MQ=255)
                                                                                     gCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctctc               >  1:181836/1‑100 (MQ=255)
                                                                                      cGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctctc               >  2:1773211/1‑99 (MQ=255)
                                                                                      cGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATctctct              >  1:1075259/1‑100 (MQ=255)
                                                                                      aaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATctctct              >  1:1860226/6‑100 (MQ=255)
                                                                                      aaagggCAACGGTTTACCGTTGCCCTGTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATatctct              >  1:1478487/6‑100 (MQ=255)
                                                                                        agggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg            >  1:728026/4‑100 (MQ=255)
                                                                                        agggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg            >  1:2646091/4‑100 (MQ=255)
                                                                                        agggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg            >  2:2615075/4‑100 (MQ=255)
                                                                                        aTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGTGGCTTACTATAtctctg               >  2:1394142/1‑96 (MQ=255)
                                                                                        aTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg            <  2:1329011/100‑1 (MQ=255)
                                                                                         gggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTggg           >  1:3126865/3‑100 (MQ=255)
                                                                                         gggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGAGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCAggg           >  1:375972/3‑100 (MQ=255)
                                                                                          ggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa          >  2:1898428/2‑100 (MQ=255)
                                                                                          ggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa          <  2:3126892/99‑1 (MQ=255)
                                                                                          cGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCGTACTATATCTCTCTggat          >  1:1472401/1‑98 (MQ=255)
                                                                                          cGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa          <  2:1450267/100‑1 (MQ=255)
                                                                                           gCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGat         >  1:2198323/1‑100 (MQ=255)
                                                                                           gCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGat         <  1:73782/100‑1 (MQ=255)
                                                                                           gCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTAGGat         >  1:2010438/1‑100 (MQ=255)
                                                                                             aaCGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGatat       >  1:2445816/1‑100 (MQ=255)
                                                                                               cGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCg     <  1:2475443/100‑1 (MQ=255)
                                                                                               cGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCg     <  2:223459/100‑1 (MQ=255)
                                                                                               cGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGAAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCg     <  1:2198724/100‑1 (MQ=255)
                                                                                                 gTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg   <  2:471755/100‑1 (MQ=255)
                                                                                                 gTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg   <  2:2114865/100‑1 (MQ=255)
                                                                                                  tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAgg  <  2:874022/100‑2 (MQ=255)
                                                                                                   |                                                                                                  
ACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGT  >  NC_000913/3291010‑3291207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: