Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,291,110 A→T 16.7% K766I (AAA→ATA) ‡ yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,1100AT16.7% 335.1 / 52.9 132K766I (AAA→ATA) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (58/52);  new base T (12/10);  total (70/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 133 total aligned reads displayed.

CACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGT  >  NC_000913/3291011‑3291207
                                                                                                   |                                                                                                 
cacaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                   <  1:507786/100‑1 (MQ=255)
   aGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAAcc                                                                                                <  2:2900755/100‑1 (MQ=255)
      ttGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACTGTAAACCGtt                                                                                             >  1:1037423/1‑100 (MQ=255)
       tgtgGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTg                                                                                            >  2:542355/1‑100 (MQ=255)
         tggtgCCCAACAGAGGTGCAATGGGAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcg                                                                                          <  1:1430777/100‑2 (MQ=255)
          ggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGccc                                                                                         >  2:3245826/1‑100 (MQ=255)
          ggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGccc                                                                                         <  1:3353730/100‑1 (MQ=255)
           gtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgat                                                                                        <  2:2093713/100‑4 (MQ=255)
           gtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgat                                                                                        <  2:2821747/100‑4 (MQ=255)
           gtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgat                                                                                        <  1:214643/100‑4 (MQ=255)
            tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatc                                                                                       <  1:3039433/100‑5 (MQ=255)
            tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatc                                                                                       <  2:1347637/100‑5 (MQ=255)
            tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCtt                                                                                       >  1:2545220/1‑100 (MQ=255)
             gCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCttt                                                                                      >  2:3230440/1‑100 (MQ=255)
              cccAGCAGAGGTGCAATGGTAGCGGCCCGTTTTACCGCCAATATCGGTTATCCCGTACTTGTTACAGTCAGCGATCGCAACGTTTTTCCGTTGcg                                                                                          <  1:173785/95‑2 (MQ=255)
              cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatcgc                                                                                     <  2:1926782/100‑7 (MQ=255)
               ccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTgg                                                                                    <  2:3211111/100‑1 (MQ=255)
                cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgc                                                                                   <  2:2740367/100‑1 (MQ=255)
                 aaCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgcg                                                                                  <  2:3153035/100‑1 (MQ=255)
                      agGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCtt                                                                             <  1:2155865/100‑1 (MQ=255)
                       ggTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTg                                                                            >  2:2475462/1‑100 (MQ=255)
                       ggTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTg                                                                            <  2:1731774/100‑1 (MQ=255)
                        gTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                           <  2:2898879/100‑1 (MQ=255)
                        gTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                           >  2:2447244/1‑100 (MQ=255)
                           cAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAg                                                                        >  1:1076560/1‑100 (MQ=255)
                           cAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAg                                                                        >  1:244646/1‑100 (MQ=255)
                               ggTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                    >  2:3028024/1‑100 (MQ=255)
                               ggTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                    <  1:1103673/100‑1 (MQ=255)
                               ggTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                    >  2:1444049/1‑100 (MQ=255)
                               ggTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGAGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                    >  1:812942/1‑100 (MQ=255)
                                gTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACg                                                                   >  2:3344815/1‑100 (MQ=255)
                                   gtggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGACACCGAACGCAACGGCAAACCGTTGACCTTTGGCGCTCTTGCCAGCAACg                                                                   >  1:3250317/3‑97 (MQ=255)
                                   gcggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGAGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatg                                                                >  1:2229170/1‑100 (MQ=255)
                                      gcgcGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATa                                                             <  2:1847836/100‑1 (MQ=255)
                                         cgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgg                                                          >  1:1067069/1‑100 (MQ=255)
                                         cgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgg                                                          >  1:101793/1‑100 (MQ=255)
                                          gTTTCAACGCCATTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACggg                                                         >  1:223459/1‑100 (MQ=255)
                                          gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACggg                                                         >  2:3088726/1‑100 (MQ=255)
                                           tttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                        >  1:1221076/1‑100 (MQ=255)
                                           tttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCATTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                        <  1:2504584/100‑1 (MQ=255)
                                            ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                       >  2:2618615/1‑100 (MQ=255)
                                            ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                       >  2:660563/1‑100 (MQ=255)
                                            ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                       >  1:914204/1‑100 (MQ=255)
                                             tCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGGCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGca                                                      <  1:3090764/100‑1 (MQ=255)
                                             tCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGca                                                      <  2:1463001/100‑1 (MQ=255)
                                               aaCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGcaac                                                    <  2:1583977/100‑1 (MQ=255)
                                                 cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGcaacaa                                                  >  1:2885967/1‑100 (MQ=255)
                                                   ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                <  1:2469415/100‑1 (MQ=255)
                                                   ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                <  2:3044922/100‑1 (MQ=255)
                                                   ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                <  1:1517475/100‑1 (MQ=255)
                                                   ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                <  2:467922/100‑1 (MQ=255)
                                                     aaTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCGCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTa                                              <  1:1046383/100‑1 (MQ=255)
                                                     aaTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTa                                              >  1:3028138/1‑100 (MQ=255)
                                                             ttATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGAt                                      <  2:1585506/100‑1 (MQ=255)
                                                              tATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATg                                     >  1:3308349/1‑100 (MQ=255)
                                                                    tctcTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcg                               <  2:1336534/97‑1 (MQ=255)
                                                                     tACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcgg                              <  2:895641/100‑1 (MQ=255)
                                                                     tACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcgg                              >  2:3187197/1‑100 (MQ=255)
                                                                       cTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCa                            >  2:1012389/1‑100 (MQ=255)
                                                                         tGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATa                          <  2:613780/100‑1 (MQ=255)
                                                                         tGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATa                          <  1:767674/100‑1 (MQ=255)
                                                                          gTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATAc                         <  2:1291560/100‑1 (MQ=255)
                                                                          gTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATAc                         >  1:1838096/1‑100 (MQ=255)
                                                                            tACAGTCAGCGTTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACTATGGTACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATa                          <  1:769079/97‑1 (MQ=255)
                                                                            tACAGTCAGCGATCGCAACGGTAAACCGTTGTCCTTTGGCGGTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                       <  1:1528359/100‑1 (MQ=255)
                                                                            tACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                       <  2:79182/100‑1 (MQ=255)
                                                                             aCAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtat                      >  1:3154160/1‑100 (MQ=255)
                                                                             aCAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtat                      <  2:2713461/100‑1 (MQ=255)
                                                                             aCAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtac                      >  1:536191/1‑99 (MQ=255)
                                                                              naGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata                     >  1:1910544/2‑100 (MQ=255)
                                                                              cAGTCAGCTATCGCAACGGTAAACCTTTCCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATTAGGGCGGCATACtat                      <  1:3075937/99‑1 (MQ=255)
                                                                              cAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGCTACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata                     >  1:2439469/1‑100 (MQ=255)
                                                                              cAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata                     >  1:364198/1‑100 (MQ=255)
                                                                                gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtc                   <  2:1160476/100‑1 (MQ=255)
                                                                                gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtc                   >  2:3130623/1‑100 (MQ=255)
                                                                                gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTACCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtc                   >  2:2570752/1‑100 (MQ=255)
                                                                                  cAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctc                 <  2:2521111/100‑1 (MQ=255)
                                                                                  cAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctc                 >  2:1220614/1‑100 (MQ=255)
                                                                                   ccaaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGCATCGTCGATGAGGGCGGCATACTATAtctct                >  1:519656/8‑100 (MQ=255)
                                                                                   ccaaagggCAACGGTTTACCGTTGCCATTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtct                  >  2:268623/8‑98 (MQ=38)
                                                                                    gCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctctc               >  1:181836/1‑100 (MQ=255)
                                                                                     aaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATctctct              >  1:1860226/6‑100 (MQ=255)
                                                                                       agggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg            >  2:2615075/4‑100 (MQ=255)
                                                                                       agggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg            >  1:2646091/4‑100 (MQ=255)
                                                                                       aTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGTGGCTTACTATAtctctg               >  2:1394142/1‑96 (MQ=255)
                                                                                        gggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTggg           >  1:3126865/3‑100 (MQ=255)
                                                                                        gggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGAGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCAggg           >  1:375972/3‑100 (MQ=255)
                                                                                         ggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa          >  2:1898428/2‑100 (MQ=255)
                                                                                         cGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCGTACTATATCTCTCTggat          >  1:1472401/1‑98 (MQ=255)
                                                                                         cGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa          <  2:1450267/100‑1 (MQ=255)
                                                                                         cGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa          >  1:1450857/1‑100 (MQ=255)
                                                                                          gCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGat         >  1:2198323/1‑100 (MQ=255)
                                                                                          gCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGat         <  1:73782/100‑1 (MQ=255)
                                                                                          gCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTAGGat         >  1:2010438/1‑100 (MQ=255)
                                                                                             aCGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATc      >  1:1933992/1‑100 (MQ=255)
                                                                                              cGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCg     <  2:223459/100‑1 (MQ=255)
                                                                                              cGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCg     <  1:2475443/100‑1 (MQ=255)
                                                                                                gTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg   <  2:471755/100‑1 (MQ=255)
                                                                                                gTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg   <  2:2114865/100‑1 (MQ=255)
                                                                                                 tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAgg  <  2:874022/100‑2 (MQ=255)
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CACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGT  >  NC_000913/3291011‑3291207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: