Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,357 C→T 15.6% intergenic (‑28/+43) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3570CT15.6% 336.8 / 47.7 141intergenic (‑28/+43)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base C (67/52);  new base T (14/8);  total (81/60)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01

Only 100 of 146 total aligned reads displayed.

CCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCAT  >  NC_000913/3471259‑3471455
                                                                                                  |                                                                                                  
 cGTGGTCAACGTGGCCGCTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTcc                                                                                                    <  1:3192730/98‑1 (MQ=255)
      tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                             <  2:61616/100‑1 (MQ=255)
      tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                             <  1:1751032/100‑1 (MQ=255)
       cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTTTTTTTTTTTTTCTATATTCATTCCTATAATGCTCTCCCCTTc                                                                                               >  1:404030/1‑97 (MQ=14)
       cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                            >  1:360676/1‑100 (MQ=255)
       cAACGTGGCCGATAGTACCAACGTTAACATGCGGATTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                            >  1:1833196/1‑100 (MQ=255)
        aaCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGga                                                                                           >  1:2443179/1‑100 (MQ=255)
          cGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                            >  2:1841642/1‑97 (MQ=255)
          cGTGGCCGATAGTACCAACGTTAACGTGCGGAATTGTACGTTCAAATTTTTCTTTAGCCACGGCTATCTCCCTGACTATAGTGCTCTCCCCTTCAGgaga                                                                                         >  1:619671/1‑100 (MQ=25)
           gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                        >  2:3289553/1‑100 (MQ=255)
            tGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagaga                                                                                       >  2:2335855/1‑100 (MQ=255)
             ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                      <  2:2053012/100‑1 (MQ=255)
             ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                      <  2:2158875/100‑1 (MQ=255)
             ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                      >  1:2725654/1‑100 (MQ=255)
               ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                                                    >  2:3179123/1‑100 (MQ=255)
                cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTTTAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                   <  2:1171369/100‑1 (MQ=21)
                cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                   <  2:1795507/100‑1 (MQ=25)
                cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCAc                                                                                   >  2:2526433/1‑100 (MQ=255)
                 gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAct                                                                                  <  1:2017576/100‑2 (MQ=25)
                 gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACg                                                                                  >  2:390705/1‑100 (MQ=255)
                     gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                              >  2:1193983/1‑100 (MQ=255)
                     gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                              <  2:639848/100‑1 (MQ=255)
                       aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                            >  1:2913572/1‑100 (MQ=255)
                       aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACt                                                                             >  1:1116907/1‑99 (MQ=255)
                        ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActatagtaa                                                                           <  2:1922769/100‑9 (MQ=25)
                         cAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTg                                                                          <  1:257785/100‑1 (MQ=255)
                          aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACCCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                         >  1:3056560/1‑100 (MQ=255)
                           aCGTTAACGTGCGGTTTTGTTCTTCCCATTTTTTCTTTAGACACGGCTATATTCCTCACTATAGTGTTCTCCCCTTCAGGTGATAGCACGGGACTTTGGt                                                                        <  1:1483540/100‑1 (MQ=255)
                            cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                       <  2:1473150/100‑1 (MQ=255)
                             gTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                       <  1:465217/99‑1 (MQ=255)
                                aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTaa                                                                   >  2:1111998/1‑100 (MQ=255)
                                   gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCGGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                >  2:3311229/1‑100 (MQ=255)
                                     gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                 >  2:529614/1‑97 (MQ=255)
                                     gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                              >  1:2216126/1‑100 (MQ=255)
                                      cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                             >  2:1139065/1‑100 (MQ=255)
                                       ggTTTTGTACGTTCAAATTTTTCTCTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                            <  1:2991634/100‑1 (MQ=255)
                                        gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                            >  1:2780884/1‑99 (MQ=255)
                                        gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCATTAgg                                                           >  1:2577201/1‑100 (MQ=255)
                                            tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGTAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCtt                                                        >  2:1339960/1‑99 (MQ=255)
                                              tACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAtt                                                     <  2:1350545/100‑1 (MQ=255)
                                                cGTTCAAATTTTTCTTTAGACACGGCTATATTCTTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGCCTTTGGTATTAACCCTTAGGCTTAtt                                                     <  2:3133910/98‑1 (MQ=255)
                                                cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGATCACGGGACTTTGGTATTATCCCTTAGGCTTATTTa                                                   >  1:2318028/1‑100 (MQ=255)
                                                cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTa                                                   >  1:3323520/1‑100 (MQ=255)
                                                 gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                  >  1:534568/1‑100 (MQ=255)
                                                    cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                               <  2:408448/100‑1 (MQ=255)
                                                      aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCGTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                             <  1:2258326/100‑1 (MQ=255)
                                                        tttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGTAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                           >  1:131165/1‑100 (MQ=255)
                                                        tttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                           <  1:1054712/100‑1 (MQ=255)
                                                         ttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                          <  1:1231173/100‑1 (MQ=255)
                                                           ttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTc                                        >  1:1774941/1‑100 (MQ=255)
                                                            tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                       >  2:480631/1‑100 (MQ=255)
                                                             cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTTGGCTTATTTACCACGGGCTTCaa                                      >  1:1795073/1‑100 (MQ=255)
                                                                  gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                 <  2:2234750/100‑1 (MQ=255)
                                                                   acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                <  2:745601/100‑1 (MQ=255)
                                                                    cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                               <  1:1911692/100‑1 (MQ=255)
                                                                     acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                              <  2:2351524/100‑1 (MQ=255)
                                                                       ggCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                            <  1:116269/100‑1 (MQ=255)
                                                                        gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                            <  1:642326/99‑1 (MQ=255)
                                                                        gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                           >  2:2759266/1‑100 (MQ=255)
                                                                        gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                           <  1:2985574/100‑1 (MQ=255)
                                                                         cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                          >  2:1559857/1‑100 (MQ=255)
                                                                         cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                          <  1:994700/100‑1 (MQ=255)
                                                                         cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                          >  2:2670290/1‑100 (MQ=255)
                                                                             atTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAAc                      <  2:252907/100‑1 (MQ=255)
                                                                                ccgtACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                   >  1:2829567/4‑100 (MQ=255)
                                                                                ccTTATTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                   <  1:1693185/100‑1 (MQ=255)
                                                                                ccTTACTATCGTGCTCTCCCCTTCAGCAGAGAGCCCGGGACTTTGGTCTTCACCCTTAGTCTTATTTACCACGCGCTTCAATTACGGCCTGACCAACg                     >  1:2881381/1‑98 (MQ=255)
                                                                                ccTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTATGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                   <  2:2829590/100‑1 (MQ=255)
                                                                                  ttACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTAc                 >  2:1834415/1‑100 (MQ=255)
                                                                                      tcccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCgg             >  2:1079229/5‑100 (MQ=37)
                                                                                       cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTCCCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg              >  1:1432459/4‑98 (MQ=37)
                                                                                       cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc            >  2:1713016/4‑100 (MQ=38)
                                                                                       ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCgg             >  1:2352278/1‑99 (MQ=255)
                                                                                       ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc            <  2:2216141/100‑1 (MQ=255)
                                                                                       ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc            >  1:2638369/1‑100 (MQ=255)
                                                                                        ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg           >  2:3249013/3‑100 (MQ=38)
                                                                                         cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc          >  2:2330156/2‑100 (MQ=38)
                                                                                         cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc          >  1:167390/2‑100 (MQ=38)
                                                                                         cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc          >  2:413717/2‑100 (MQ=38)
                                                                                         aGTTCTCTCCCCTGCAGGAGAGAGCACGCGACTTTGGTATTAACACTTAGGCTTATTTACTACGGGCTTCAATTACGATCTCAACAACGTTCCTCGgcgc          >  1:608032/1‑100 (MQ=255)
                                                                                          gTGCTCTCTCCTGAAGGGGAGAGCACGGGAATTTGGTATTAACCCTTAGGGTTATTTACCACGGGCTTCAATTACGCCTTGAGCAACg                     >  1:2442787/1‑88 (MQ=25)
                                                                                          gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTGCCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt         <  1:1892735/100‑1 (MQ=255)
                                                                                          gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt         >  1:2024634/1‑100 (MQ=255)
                                                                                           tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt        >  2:1616613/1‑100 (MQ=255)
                                                                                           tGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt        >  2:2293978/1‑100 (MQ=255)
                                                                                            gCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc       >  1:2687162/1‑100 (MQ=255)
                                                                                            gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc       <  1:1820783/100‑1 (MQ=255)
                                                                                            gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc       >  2:2754145/1‑100 (MQ=255)
                                                                                            gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc       >  1:445538/1‑100 (MQ=255)
                                                                                             ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca      >  2:766646/1‑100 (MQ=255)
                                                                                             ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca      >  1:3121437/1‑100 (MQ=255)
                                                                                              tgtcCCCTTCAGGATAGTGCAGGGGACTTTGGTATTAACCCTTAGCCTTATATACCACGGGTTCCAATTACCGCCCGACCAACGTTACCCGGCGCTtcat     >  1:978504/3‑100 (MQ=255)
                                                                                              tctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat     <  2:1429905/100‑1 (MQ=255)
                                                                                              tctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat     <  2:1984152/100‑1 (MQ=255)
                                                                                               ctcCCCTTCAGGAGAGGGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCGTCAATTACAGCCTGAGCAACGATACTCGGCGCTtcat     >  2:2701169/1‑99 (MQ=255)
                                                                                               ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc    <  2:3385301/100‑1 (MQ=255)
                                                                                               ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc    <  1:67455/100‑1 (MQ=255)
                                                                                               ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc    <  2:171046/100‑1 (MQ=255)
                                                                                               ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc    >  2:2265020/1‑100 (MQ=255)
                                                                                                 ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTcatcat  >  2:2786500/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
CCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCAT  >  NC_000913/3471259‑3471455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: