Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,361 T→G 16.0% intergenic (‑32/+39) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3610TG16.0% 361.3 / 58.6 148intergenic (‑32/+39)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base T (70/53);  new base G (15/9);  total (85/63)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-01

Only 100 of 150 total aligned reads displayed.

GGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTC  >  NC_000913/3471263‑3471460
                                                                                                  |                                                                                                   
ggTCAACGTGGCCGATAGTACCAGCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTc                                                                                                    <  1:2744632/100‑1 (MQ=255)
  tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                                  <  1:2057539/100‑1 (MQ=255)
  tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                                  <  2:61616/100‑1 (MQ=255)
  tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                                  <  1:1751032/100‑1 (MQ=255)
   cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTTTTTTTTTTTTTCTATATTCATTCCTATAATGCTCTCCCCTTc                                                                                                    >  1:404030/1‑97 (MQ=14)
   cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                                 >  1:360676/1‑100 (MQ=255)
   cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                                 >  2:221054/1‑100 (MQ=255)
    aaCGTGGCCGATAGTACCAACTTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACTCGGGTATATTCCTTACTATTGTGCTCTCCCCTTCAGga                                                                                                <  1:542406/100‑1 (MQ=21)
      cGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                                 >  2:1841642/1‑97 (MQ=255)
       gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                             <  2:2321363/100‑1 (MQ=255)
        tGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagaga                                                                                            >  2:2335855/1‑100 (MQ=255)
         ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                           >  1:2725654/1‑100 (MQ=255)
         ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                           <  2:2158875/100‑1 (MQ=255)
          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                          <  2:1034377/100‑1 (MQ=255)
           ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCa                                                                                         <  1:3011959/100‑1 (MQ=25)
            cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTTTAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                        <  2:1171369/100‑1 (MQ=21)
            cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                        <  2:1795507/100‑1 (MQ=25)
            cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCAc                                                                                        >  2:2526433/1‑100 (MQ=255)
             gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAct                                                                                       <  1:2017576/100‑2 (MQ=25)
             gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACg                                                                                       >  2:390705/1‑100 (MQ=255)
                 gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                   <  2:639848/100‑1 (MQ=255)
                 gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                   >  2:1193983/1‑100 (MQ=255)
                  tACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACt                                                                                  >  1:2657621/1‑100 (MQ=255)
                   aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                                 >  1:2913572/1‑100 (MQ=255)
                   aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACt                                                                                  >  1:1116907/1‑99 (MQ=255)
                    ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActatagtaa                                                                                <  2:1997983/100‑9 (MQ=25)
                      aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACCCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                              >  1:3056560/1‑100 (MQ=255)
                      aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                              >  2:2770053/1‑100 (MQ=255)
                       aCGTTAACGTGCGGTTTTGTTCTTCCCATTTTTTCTTTAGACACGGCTATATTCCTCACTATAGTGTTCTCCCCTTCAGGTGATAGCACGGGACTTTGGt                                                                             <  1:1483540/100‑1 (MQ=255)
                       aCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                             <  2:2978281/100‑1 (MQ=255)
                        cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                            <  2:1473150/100‑1 (MQ=255)
                         gTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                            <  1:465217/99‑1 (MQ=255)
                            aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTaa                                                                        >  2:1111998/1‑100 (MQ=255)
                             aCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAc                                                                       <  1:1833272/100‑1 (MQ=255)
                               gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCGGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                     >  2:3311229/1‑100 (MQ=255)
                                 gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                      >  2:529614/1‑97 (MQ=255)
                                 gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                                   >  1:2216126/1‑100 (MQ=255)
                                  cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                  >  2:1139065/1‑100 (MQ=255)
                                    gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCATTAgg                                                                >  1:2577201/1‑100 (MQ=255)
                                      tttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGc                                                               >  2:1922282/1‑99 (MQ=255)
                                        tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGTAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCtt                                                             >  2:1339960/1‑99 (MQ=255)
                                          tACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAtt                                                          <  2:1350545/100‑1 (MQ=255)
                                            cGTTCAAATTTTTCTTTAGACACGGCTATATTCTTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGCCTTTGGTATTAACCCTTAGGCTTAtt                                                          <  2:3133910/98‑1 (MQ=255)
                                            cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGATCACGGGACTTTGGTATTATCCCTTAGGCTTATTTa                                                        >  1:2318028/1‑100 (MQ=255)
                                              ttCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAcc                                                      <  1:3169056/100‑1 (MQ=255)
                                                cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                    <  2:408448/100‑1 (MQ=255)
                                                  aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                  >  1:454853/1‑100 (MQ=255)
                                                    tttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGTAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                >  1:131165/1‑100 (MQ=255)
                                                    tttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                <  1:1054712/100‑1 (MQ=255)
                                                    tttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTGCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                <  2:3205952/100‑1 (MQ=255)
                                                     ttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                               <  1:1231173/100‑1 (MQ=255)
                                                       ttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTc                                             >  1:1774941/1‑100 (MQ=255)
                                                        tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                            <  1:1592996/100‑1 (MQ=255)
                                                        tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                            >  2:480631/1‑100 (MQ=255)
                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTTGGCTTATTTACCACGGGCTTCaa                                           >  1:1795073/1‑100 (MQ=255)
                                                             aGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTACAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTATTTACCACGGGCTTCAATTACg                                      <  1:2282972/100‑1 (MQ=255)
                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                      <  2:2234750/100‑1 (MQ=255)
                                                               acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                     <  2:745601/100‑1 (MQ=255)
                                                                 acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                   <  2:2351524/100‑1 (MQ=255)
                                                                    gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                                 <  1:642326/99‑1 (MQ=255)
                                                                    gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                                <  1:2985574/100‑1 (MQ=255)
                                                                    gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                                >  2:2759266/1‑100 (MQ=255)
                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                               >  2:1559857/1‑100 (MQ=255)
                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                               >  2:2670290/1‑100 (MQ=255)
                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                               <  1:994700/100‑1 (MQ=255)
                                                                         atTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAAc                           <  2:252907/100‑1 (MQ=255)
                                                                           tCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAAACATAGGCTTATTTACCACGGGCTTCCATTACGGACTAAGAAACGt                         <  1:1339954/100‑1 (MQ=255)
                                                                            ccgtACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                        >  1:2829567/4‑100 (MQ=255)
                                                                            ccTTATTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                        <  1:1693185/100‑1 (MQ=255)
                                                                            ccTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTATGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                        <  2:2829590/100‑1 (MQ=255)
                                                                             cTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGTACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTa                       >  1:2436124/1‑100 (MQ=255)
                                                                               tACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACt                     >  1:62321/1‑100 (MQ=255)
                                                                                aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                    >  1:2474193/1‑100 (MQ=255)
                                                                                   cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                 >  2:1713016/4‑100 (MQ=38)
                                                                                   ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                 >  1:1944643/1‑100 (MQ=255)
                                                                                   ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                 >  1:2638369/1‑100 (MQ=255)
                                                                                    ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                >  2:3249013/3‑100 (MQ=38)
                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCACTTACGGCCTGAGCAACGTTACTc                    >  1:2852585/2‑95 (MQ=38)
                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc               >  1:167390/2‑100 (MQ=38)
                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc               >  2:413717/2‑100 (MQ=38)
                                                                                     aGTTCTCTCCCCTGCAGGAGAGAGCACGCGACTTTGGTATTAACACTTAGGCTTATTTACTACGGGCTTCAATTACGATCTCAACAACGTTCCTCGgcgc               >  1:608032/1‑100 (MQ=255)
                                                                                     aGTGCTCTCCCCTTCTGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc               <  2:3331786/100‑1 (MQ=255)
                                                                                      gTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt              >  1:766954/1‑100 (MQ=39)
                                                                                      gTGCTCTCTCCTGAAGGGGAGAGCACGGGAATTTGGTATTAACCCTTAGGGTTATTTACCACGGGCTTCAATTACGCCTTGAGCAACg                          >  1:2442787/1‑88 (MQ=25)
                                                                                      gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTGCCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt              <  1:1892735/100‑1 (MQ=255)
                                                                                       tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt             >  2:1616613/1‑100 (MQ=255)
                                                                                       tGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt             >  2:2293978/1‑100 (MQ=255)
                                                                                        gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc            >  2:2754145/1‑100 (MQ=255)
                                                                                        gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc            <  1:1820783/100‑1 (MQ=255)
                                                                                         ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca           >  2:766646/1‑100 (MQ=255)
                                                                                         ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca           >  2:2238457/1‑100 (MQ=255)
                                                                                         ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca           <  2:1824152/100‑1 (MQ=255)
                                                                                          tctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat          <  2:1984152/100‑1 (MQ=255)
                                                                                          tctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat          <  2:1429905/100‑1 (MQ=255)
                                                                                           ctcCCCTTCAGGAGAGGGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCGTCAATTACAGCCTGAGCAACGATACTCGGCGCTtcat          >  2:2701169/1‑99 (MQ=255)
                                                                                           ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc         <  2:3385301/100‑1 (MQ=255)
                                                                                           ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc         <  2:171046/100‑1 (MQ=255)
                                                                                              cccTTCAGGAGAGAGCACGGGACGTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatca        <  1:1203279/98‑1 (MQ=255)
                                                                                               ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc     <  1:88075/100‑1 (MQ=255)
                                                                                                cTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACt    <  1:3140759/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTC  >  NC_000913/3471263‑3471460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: