Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,434 T→C 63.8% intergenic (‑15/‑206) fkpA ← / → slyX FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,4340TC63.8% 129.2 / 117.3 132intergenic (‑15/‑206)fkpA/slyXFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein
Reads supporting (aligned to +/- strand):  ref base T (17/30);  new base C (37/45);  total (56/76)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTC  >  NC_000913/3477339‑3477533
                                                                                               |                                                                                                   
ttCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCggg                                                                                                 <  2:459381/100‑1 (MQ=255)
ttCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGACCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCggg                                                                                                 <  1:2283565/100‑1 (MQ=255)
  cagcagCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGc                                                                                               <  2:2911677/100‑1 (MQ=255)
  cagcagCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGc                                                                                               <  2:2083710/100‑1 (MQ=255)
   agcagcAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCa                                                                                              >  2:1176693/1‑100 (MQ=255)
   agcagcAAAAGTGAGTGGTGCATGCAGGGCAACGGCGATTGTGGTCGCAAGCAGCGTTACTTTAAACAGTGATTTGATCCATAGCTCCAGGACCGGGGCa                                                                                              >  1:2596583/1‑100 (MQ=255)
    gcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCAt                                                                                             >  2:925721/1‑100 (MQ=255)
    gcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAt                                                                                             >  1:1994114/1‑100 (MQ=255)
      agcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCAtct                                                                                           >  2:1601836/1‑100 (MQ=255)
      agcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtct                                                                                           <  1:2877445/100‑1 (MQ=255)
      agcaAAAGTGATTGGTGCATCCAGGGCAACGGCCATAGTGATCGCCAGCGGCGTAACTATAAACAGTGATTGCATCCATATCTCCAGGGTCGGTGCAtct                                                                                           >  1:2936986/1‑100 (MQ=255)
       gcaAAAGTGATTGGTGCATGCAGGGCAATGGCCATTGTGGGCTCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCAtct                                                                                           <  1:1046776/99‑1 (MQ=255)
       gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                          <  2:2848032/100‑1 (MQ=255)
       gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                          <  1:1331899/100‑1 (MQ=255)
       gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                          <  2:1825009/100‑1 (MQ=255)
        caAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCa                                                                                         <  2:3269043/100‑1 (MQ=255)
         aaaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAc                                                                                        >  1:572628/1‑100 (MQ=255)
         aaaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAc                                                                                        >  1:2867559/1‑100 (MQ=255)
          aaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAcc                                                                                       <  2:511495/100‑1 (MQ=255)
               gATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAgg                                                                                  >  1:3082621/1‑100 (MQ=255)
                aTTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAggg                                                                                 <  2:2858759/100‑1 (MQ=255)
                aTTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAggg                                                                                 <  2:2183236/100‑1 (MQ=255)
                  tGGTGCATGCAGGGCGATGGCCATTGTGGGCGCAAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCAgccc                                                                                     <  1:2847419/94‑1 (MQ=255)
                  tGGTGCATGCAGCGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTTATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGtt                                                                               <  1:1852671/100‑1 (MQ=255)
                   ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                              >  2:2176497/1‑100 (MQ=255)
                   ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                              >  2:1991194/1‑100 (MQ=255)
                   ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                              <  2:252887/100‑1 (MQ=255)
                   ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                              >  1:3282018/1‑100 (MQ=255)
                    gTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTaa                                                                             >  2:2824928/1‑100 (MQ=255)
                    gTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTaa                                                                             >  2:1789454/1‑100 (MQ=255)
                      gcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACt                                                                           >  1:1155012/1‑100 (MQ=255)
                       catgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACta                                                                          <  2:3202920/100‑1 (MQ=255)
                         tgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtatt                                                                        >  2:2118038/1‑100 (MQ=255)
                           caGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtattat                                                                      <  2:572771/100‑1 (MQ=255)
                            aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                     <  1:489723/100‑1 (MQ=255)
                                 aaCGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAg                                                                <  2:1502597/100‑1 (MQ=255)
                                   cGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAggg                                                              <  2:938755/100‑1 (MQ=255)
                                    ggCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGt                                                             <  1:1387625/100‑1 (MQ=255)
                                     gCCATTGTGGTCGCCAGCAGCGTTACATTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTa                                                            >  2:2066425/1‑100 (MQ=255)
                                     gCCAGTGTGGTCGCCAGCAGCGTTACTTTAAGCAGTGATTTCATCCATATCTCCAAGACCGGGGCATGTCACCCCAGGGTTAACTATTATCAGAAGGGt                                                             <  1:525206/99‑1 (MQ=255)
                                      ccATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTAc                                                           >  1:1018260/1‑100 (MQ=255)
                                       cATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACt                                                          <  1:3386785/100‑1 (MQ=255)
                                         ttGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACtat                                                        <  1:3156995/100‑1 (MQ=255)
                                         ttGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACtat                                                        <  2:3249279/100‑1 (MQ=255)
                                            tgGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGATTAACTATTATCAGAAGGGTACTATaaa                                                     >  1:733227/1‑100 (MQ=255)
                                             ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCTAGGGTTAACTATTATCAGAAGGGTACTATaaa                                                     >  1:2796767/1‑99 (MQ=255)
                                             ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                    >  1:79830/1‑100 (MQ=255)
                                              gTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGc                                                   <  1:787885/100‑1 (MQ=255)
                                               tCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCg                                                  <  1:2472636/100‑1 (MQ=255)
                                                cGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt                                                 <  2:79830/100‑1 (MQ=255)
                                                  ccAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                               >  1:2067313/1‑100 (MQ=255)
                                                  ccAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                               <  2:513843/100‑1 (MQ=255)
                                                     gcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCg                                            <  2:213779/100‑1 (MQ=255)
                                                     gcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCg                                            <  2:1155232/100‑1 (MQ=255)
                                                       agcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGaa                                          <  1:834451/100‑1 (MQ=255)
                                                       agcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGAACGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGaa                                          <  1:2215047/100‑1 (MQ=255)
                                                        gcGTTACTTTAAATAGTGATTTCAGCCATATCTACATGATTGGAGCATCTCAACCGAGGGGTAACTATTATCAGAAGGGTACTAGAAAGCGGTGTCg                                            <  1:692772/97‑1 (MQ=255)
                                                        gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCTGGGCATCTCACCCCAGGGATAACTATTATCAGAAGGGTACTATAAAGCGCTGTCGAAc                                         >  1:1661003/1‑100 (MQ=255)
                                                        gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                         <  1:1992145/100‑1 (MQ=255)
                                                        gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                         >  2:3013927/1‑100 (MQ=255)
                                                            tACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACACCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAAt                                     <  1:2018066/100‑1 (MQ=255)
                                                            tACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAAt                                     >  2:1520440/1‑100 (MQ=255)
                                                             aCTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    >  1:973049/1‑100 (MQ=255)
                                                             aCTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    <  1:1674454/100‑1 (MQ=255)
                                                             aCTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    <  1:1942778/100‑1 (MQ=255)
                                                              cTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                   >  1:1101407/1‑100 (MQ=255)
                                                              cTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                   >  1:1689807/1‑100 (MQ=255)
                                                               tttAAACAGTGATTTCATCCATATCTCCAGGGTCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCta                                  <  2:2210740/100‑1 (MQ=255)
                                                               tttAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCta                                  <  1:610673/100‑1 (MQ=255)
                                                               tttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCta                                  <  1:105775/100‑1 (MQ=255)
                                                                ttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtac                                 >  2:1223044/1‑100 (MQ=255)
                                                                 tAAACAGTTATTTCAGTCATATCTTCAGGACCGGGGCATCGCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtaca                                <  1:2980421/100‑1 (MQ=255)
                                                                    aCAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacatac                             <  2:878456/100‑1 (MQ=255)
                                                                     cAGTGATTTCATTCATATCTCCAGGAGCGGGGCATATCAGCCCAGGGTTGACTATTATCAGAAGGGGACTATAAAGCGTTGTCGAACAAATTttcata                              >  1:164493/1‑98 (MQ=255)
                                                                     cAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                            >  1:1865589/1‑100 (MQ=255)
                                                                     cAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                            <  1:3383744/100‑1 (MQ=255)
                                                                     cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                            >  1:2427007/1‑100 (MQ=255)
                                                                     cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                            <  2:1018263/100‑1 (MQ=255)
                                                                      aGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAg                           <  1:849003/100‑1 (MQ=255)
                                                                      aGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAg                           >  2:2064249/1‑100 (MQ=255)
                                                                      aGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAg                           <  1:1264157/100‑1 (MQ=255)
                                                                       gTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAACGCGTTGTCGAACAAATCTACATACAg                           >  2:1106783/1‑99 (MQ=255)
                                                                       gTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGa                          <  1:2149776/100‑1 (MQ=255)
                                                                        tGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGac                         <  1:2912098/100‑1 (MQ=255)
                                                                          aTTTCATCCATAACTCCAGGACCGGGGCATCTCACCCAACGGTTAACTATTACCAGAAGGGTACTATAAAGCGTGGCCTAACAAATCTACAGACAGacac                       >  1:1474521/1‑100 (MQ=255)
                                                                             tCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTATATACAGACACGcc                    <  1:2092590/100‑1 (MQ=255)
                                                                              cATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGc                     >  1:2549789/1‑98 (MQ=255)
                                                                              cATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                   <  1:856840/100‑1 (MQ=255)
                                                                              cATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                   >  2:604822/1‑100 (MQ=255)
                                                                                tCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCta                 <  1:3013901/100‑1 (MQ=255)
                                                                                 ccATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtat                <  1:1520433/100‑1 (MQ=255)
                                                                                  cATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt               >  1:114087/1‑100 (MQ=255)
                                                                                     atCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatc            <  2:2605883/100‑1 (MQ=255)
                                                                                      tctcCATGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca           <  1:2714999/100‑1 (MQ=255)
                                                                                      tctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca           <  2:390505/100‑1 (MQ=255)
                                                                                        tcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc         >  2:2673691/1‑100 (MQ=255)
                                                                                         ccAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCt        >  1:1067927/1‑100 (MQ=255)
                                                                                         ccAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCt        >  2:1752654/1‑100 (MQ=255)
                                                                                          cAGGAACGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCTAACAAATATACATACAGACACGCCATATTATCATCTa       >  1:679903/1‑100 (MQ=255)
                                                                                               tCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTc  <  1:2084104/100‑1 (MQ=255)
                                                                                               |                                                                                                   
TTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTC  >  NC_000913/3477339‑3477533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: