Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,524,413 A→G 65.0% I515V (ATT→GTT)  mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,524,4130AG65.0% 170.3 / 131.8 151I515V (ATT→GTT) mrcApenicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (23/30);  new base G (51/47);  total (74/77)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

GGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAAC  >  NC_000913/3524316‑3524509
                                                                                                 |                                                                                                
ggCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAAtt                                                                                                >  1:677324/1‑100 (MQ=255)
 gCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACTGCAGTATGCTGGTCCAGTTc                                                                                               <  2:3186994/100‑1 (MQ=255)
  cAAGTATGTTGAACGATGTGCCAATTTCTCGCTGTGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCAGGTCCAGTTCg                                                                                              <  1:1780473/100‑1 (MQ=255)
     gTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCCCCGCAGTATGCTGGTCCAGTTCGCtt                                                                                           >  1:2902225/1‑100 (MQ=255)
      tATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTa                                                                                          <  2:2974625/100‑1 (MQ=255)
      tATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTa                                                                                          <  2:40842/100‑1 (MQ=255)
      tATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTa                                                                                          <  2:716429/100‑1 (MQ=255)
       aTGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTAc                                                                                         <  1:378887/100‑1 (MQ=255)
        tGTTGAACGCTGTGCCATTTTCTTGATGTGATGCATGTACCGGTTCTGACTGTTATCCTAAGAACACACCACCGCAGTATACTGGGCCAGTTCCCtt                                                                                           >  1:623532/1‑97 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACTACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                        <  1:1524401/100‑1 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                        >  2:1343096/1‑100 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                        >  2:2412129/1‑100 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                        >  1:555768/1‑100 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                        <  1:1315290/100‑1 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACg                                                                                        <  1:1678895/100‑1 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACg                                                                                        <  1:168308/100‑1 (MQ=255)
         gTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGt                                                                                       <  1:707664/100‑1 (MQ=255)
         gTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGt                                                                                       >  2:3066587/1‑100 (MQ=255)
           tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTCACGTCa                                                                                     >  1:791205/1‑100 (MQ=255)
           tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCa                                                                                     >  1:2581950/1‑100 (MQ=255)
            gAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAg                                                                                    >  2:2157003/1‑100 (MQ=255)
            gAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAg                                                                                    <  1:1262579/100‑1 (MQ=255)
             aaCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAgg                                                                                   <  1:246976/100‑1 (MQ=255)
             aaCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAgg                                                                                   <  1:1196702/100‑1 (MQ=255)
               cGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAgggg                                                                                 <  1:1358328/100‑1 (MQ=255)
                gATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGACCTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGc                                                                                <  1:1474474/100‑1 (MQ=255)
                 aTGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCt                                                                               >  2:2787480/1‑100 (MQ=255)
                   gtgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTgg                                                                             >  1:2222113/1‑100 (MQ=255)
                    tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                            <  2:129206/100‑1 (MQ=255)
                    tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                            >  2:1502783/1‑100 (MQ=255)
                    tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                            <  1:2412111/100‑1 (MQ=255)
                    tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                            <  1:1343090/100‑1 (MQ=255)
                    tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                            <  1:2983715/100‑1 (MQ=255)
                     gCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCGCCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGt                                                                           >  2:1403489/1‑100 (MQ=255)
                     gCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGt                                                                           <  2:205676/100‑1 (MQ=255)
                     gCCAATTGCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGt                                                                           <  1:2899241/100‑1 (MQ=255)
                       cAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCa                                                                         >  2:2308222/1‑100 (MQ=255)
                        aaTTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTCTGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAg                                                                        <  1:1814656/100‑1 (MQ=255)
                        aaTTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCa                                                                         >  1:2333701/1‑99 (MQ=255)
                          tttCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTc                                                                      <  2:2098685/100‑1 (MQ=255)
                            tctcGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGTTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGa                                                                    <  1:2603747/100‑1 (MQ=255)
                             ctcGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGaa                                                                   <  1:505979/100‑1 (MQ=255)
                             ctcGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGaa                                                                   <  1:2834971/100‑1 (MQ=255)
                                  tGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTAGGCTGGTCCAATTCGCTTACGTCAGGTGCTGGGTCAGTCGAAAAACg                                                              <  1:1879422/100‑1 (MQ=255)
                                   gggCTGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACg                                                              >  1:1365771/1‑99 (MQ=255)
                                    ggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtg                                                            <  2:2467296/100‑1 (MQ=255)
                                     gATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                           <  2:252588/100‑1 (MQ=255)
                                     gATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                           >  1:2397211/1‑100 (MQ=255)
                                      aggCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtggt                                                          <  2:2338568/98‑1 (MQ=255)
                                        gCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGa                                                        <  2:2117081/100‑1 (MQ=255)
                                         cAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGAt                                                       >  1:288526/1‑100 (MQ=255)
                                         cAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGAt                                                       >  2:1520901/1‑100 (MQ=255)
                                         cAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGAt                                                       <  2:3350793/100‑1 (MQ=255)
                                         cAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGAt                                                       >  2:674132/1‑100 (MQ=255)
                                         cAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACAACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGATGGGTCAGTCGAAAAACgtggtg                                                         >  1:2013293/1‑98 (MQ=255)
                                           aGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATgg                                                     >  2:376790/1‑100 (MQ=255)
                                            gTGACGGTGCTGCCTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGt                                                    <  1:936786/100‑1 (MQ=255)
                                                   ttCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCa                                             <  2:2673454/100‑1 (MQ=255)
                                                      taaCTGGCAGCCGAAGAACTCACCACCGCAGGATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATg                                          >  2:2282522/3‑100 (MQ=255)
                                                      tGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATg                                          >  1:1008693/1‑100 (MQ=255)
                                                       gACTGGCAGCCGCAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGc                                         <  1:433470/100‑1 (MQ=255)
                                                           ggCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGc                                     >  2:1804381/1‑100 (MQ=255)
                                                           ggCAGCCGAAGAACTCACCACCGAAGTATGCTGGTCCAGTTCGCATACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGGGGGc                                     >  1:295759/1‑100 (MQ=255)
                                                            gCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGCGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCg                                    >  1:1629068/1‑100 (MQ=255)
                                                             cAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGa                                   >  2:1037215/1‑100 (MQ=255)
                                                             cAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGa                                   >  2:1989539/1‑100 (MQ=255)
                                                             cAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGa                                   >  2:2698079/1‑100 (MQ=255)
                                                             cAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTACGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGa                                   >  1:2083020/1‑100 (MQ=255)
                                                              aGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGGCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGAt                                  <  1:2469381/100‑1 (MQ=255)
                                                               gCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATg                                 >  2:1221003/1‑100 (MQ=255)
                                                               gCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATg                                 >  1:3209190/1‑100 (MQ=255)
                                                               gCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATg                                 <  2:287575/100‑1 (MQ=255)
                                                                  gaagaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGCCTGGGTCAGACGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATagg                               >  1:3047330/1‑99 (MQ=255)
                                                                   aagaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCg                             >  1:2221451/1‑100 (MQ=255)
                                                                   aagaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCg                             <  1:475428/100‑1 (MQ=255)
                                                                     gaaCTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTAGTGATGGTACGCGCAATGCGGGCGATGGGCGTc                           >  1:2274729/1‑100 (MQ=255)
                                                                      aaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCg                          <  2:1842847/100‑1 (MQ=255)
                                                                        cTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAc                        >  2:2781868/1‑100 (MQ=255)
                                                                         tCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGCCGATGGGCGTCGACt                       >  2:3337640/1‑100 (MQ=255)
                                                                         tCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAcn                       <  2:2888700/100‑2 (MQ=255)
                                                                          caccacCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                      >  1:1155049/1‑100 (MQ=255)
                                                                          caccacCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                      <  2:492206/100‑1 (MQ=255)
                                                                          caccacCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                      >  1:1212218/1‑100 (MQ=255)
                                                                          caccacCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                      >  2:1704878/1‑100 (MQ=255)
                                                                           accaccGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGTCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTAc                     >  2:292705/1‑100 (MQ=255)
                                                                             caccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGc                   >  1:1392949/1‑100 (MQ=255)
                                                                              accGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGAAGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCt                  >  2:609497/1‑100 (MQ=255)
                                                                               ccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTg                 >  2:3237853/1‑100 (MQ=255)
                                                                                   aGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGa             <  2:3047356/100‑1 (MQ=255)
                                                                                     tATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAat           <  2:1138454/100‑1 (MQ=255)
                                                                                      aTGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAata          <  1:607932/100‑1 (MQ=255)
                                                                                      aTGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAata          <  1:725181/100‑1 (MQ=255)
                                                                                       tGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAatat         >  2:1865517/1‑100 (MQ=255)
                                                                                        gCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAatat         >  1:1756182/1‑99 (MQ=255)
                                                                                         cTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCt       >  1:917326/1‑100 (MQ=255)
                                                                                            gTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCa    >  2:2191442/1‑100 (MQ=255)
                                                                                            gTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCa    <  2:927399/100‑1 (MQ=255)
                                                                                             tCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCaa   >  2:530977/1‑100 (MQ=255)
                                                                                             tCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCa    >  1:1250176/1‑99 (MQ=255)
                                                                                              ccAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAAc  <  2:642325/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAAC  >  NC_000913/3524316‑3524509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: