Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,971 C→A 24.1% intergenic (‑181/+127) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9710CA24.1% 320.9 / 98.6 137intergenic (‑181/+127)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (47/57);  new base A (29/4);  total (76/61)
Fisher's exact test for biased strand distribution p-value = 1.40e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-01

Only 100 of 146 total aligned reads displayed.

GGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCT  >  NC_000913/3705873‑3706070
                                                                                                  |                                                                                                         
gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                          >  1:3385468/1‑100 (MQ=255)
  gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAAGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                        >  1:1808223/1‑100 (MQ=255)
   aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                       <  2:1785681/100‑1 (MQ=255)
   aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                       <  2:3017071/100‑1 (MQ=255)
   aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                       <  2:560399/100‑1 (MQ=255)
     tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                     >  1:3262150/1‑100 (MQ=255)
      gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                    >  2:3351893/1‑100 (MQ=255)
       aaGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                   >  1:1641763/1‑100 (MQ=255)
         gTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                                 >  1:780706/1‑100 (MQ=255)
           aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                               <  2:604557/100‑1 (MQ=255)
             tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                             >  2:3169757/1‑100 (MQ=255)
              gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACACAGTGCGCTCTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                            >  1:2775941/1‑100 (MQ=255)
              gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                            >  1:1070749/1‑100 (MQ=255)
               ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                           >  2:525132/1‑100 (MQ=255)
                    ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                      >  2:2292391/1‑100 (MQ=255)
                     gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTGGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                     <  1:1656019/100‑2 (MQ=37)
                      aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                    <  1:1539511/100‑1 (MQ=255)
                      aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                    <  2:1617370/100‑3 (MQ=37)
                       tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                   >  1:2772246/1‑100 (MQ=255)
                        ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                  >  2:1049306/1‑100 (MQ=255)
                         gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                >  2:1976710/1‑100 (MQ=255)
                           gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                              >  2:453470/1‑100 (MQ=255)
                           gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCATTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                              >  1:1595649/1‑100 (MQ=255)
                             tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                            <  1:481235/100‑1 (MQ=255)
                             tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                            <  2:2857025/100‑1 (MQ=255)
                                 cgtagagGCACAGCACCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        <  1:1075305/97‑1 (MQ=255)
                                 cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        >  1:3245450/1‑100 (MQ=255)
                                 cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        <  1:489847/100‑1 (MQ=255)
                                  gtagagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                       <  1:1246876/98‑1 (MQ=255)
                                  gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                       <  2:303189/100‑1 (MQ=255)
                                  gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                       <  1:1576694/100‑1 (MQ=255)
                                  gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                       <  1:1246125/100‑1 (MQ=255)
                                   cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                      <  2:3345516/100‑1 (MQ=255)
                                   cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                      <  1:2412074/100‑1 (MQ=255)
                                   cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                      >  1:415854/1‑100 (MQ=255)
                                    agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                      <  1:317922/99‑1 (MQ=255)
                                      agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                   <  2:1376918/100‑1 (MQ=255)
                                        gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                 <  1:708013/100‑1 (MQ=255)
                                         cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                <  2:1070752/100‑1 (MQ=255)
                                          acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                               >  2:652931/1‑100 (MQ=255)
                                               gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                          >  2:2882506/1‑100 (MQ=255)
                                               gcCACCGGGTATACAGATTCTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                          <  1:360147/100‑1 (MQ=255)
                                                ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                         >  2:2048219/1‑100 (MQ=255)
                                                   ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                      <  1:1618408/100‑1 (MQ=255)
                                                    cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                    >  1:1872440/1‑100 (MQ=255)
                                                    cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                    <  1:2647757/100‑1 (MQ=255)
                                                     gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                   >  1:417066/1‑100 (MQ=255)
                                                          taCAGATTGTTCACAATGTTCGCTTTGTTTGTTCCGGATGCGGCGTTTAGCCCTTATCCGGTCTACAGTATCGTTCAATTTCATTTCATTGAAGAGttt                                             >  1:2597208/1‑99 (MQ=12)
                                                           aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTc                                           <  2:1808234/100‑1 (MQ=255)
                                                           aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTc                                           >  2:569526/1‑100 (MQ=255)
                                                             aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                       <  2:2698321/100‑1 (MQ=255)
                                                             aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                       <  2:92022/100‑1 (MQ=255)
                                                              gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                      <  1:1000320/100‑1 (MQ=255)
                                                               aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                      <  2:1621402/99‑1 (MQ=255)
                                                               aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAg                                     >  2:2161586/1‑100 (MQ=255)
                                                                ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                    <  2:1223669/100‑1 (MQ=255)
                                                                  gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                  >  1:2594915/1‑100 (MQ=255)
                                                                  ataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                  >  2:3185898/5‑100 (MQ=18)
                                                                   ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                 <  1:2990399/100‑1 (MQ=255)
                                                                   ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                 <  2:1715781/100‑1 (MQ=255)
                                                                    tcaCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTg                                >  2:2291088/3‑100 (MQ=255)
                                                                       cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATa                             <  1:73175/100‑1 (MQ=255)
                                                                         aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAg                           >  2:2525288/1‑100 (MQ=255)
                                                                            tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCgg                        <  1:1464915/100‑1 (MQ=255)
                                                                            tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCgg                        <  2:419628/100‑1 (MQ=255)
                                                                             gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGa                       <  1:2525268/100‑1 (MQ=255)
                                                                             gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGa                       <  2:2396043/100‑1 (MQ=255)
                                                                              cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                      >  2:1512176/1‑100 (MQ=255)
                                                                              cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                      >  2:786088/1‑100 (MQ=255)
                                                                               gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                     <  2:1545345/100‑1 (MQ=255)
                                                                               gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                     <  1:859607/100‑1 (MQ=255)
                                                                               gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                     <  1:156416/100‑1 (MQ=255)
                                                                                cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                    <  2:1265712/100‑1 (MQ=255)
                                                                                  ctttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAgcgcagcgca                  >  2:3390066/9‑100 (MQ=21)
                                                                                   tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                 >  1:2121174/1‑100 (MQ=255)
                                                                                     tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa               <  1:2396405/100‑1 (MQ=255)
                                                                                      gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  2:1291721/5‑100 (MQ=18)
                                                                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg              >  1:2412996/5‑100 (MQ=17)
                                                                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAAACCTGCAAATTCAATATATTGCAGGATACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg              >  1:3358721/5‑100 (MQ=18)
                                                                                       taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  2:3054615/4‑100 (MQ=18)
                                                                                       taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  1:2980811/4‑100 (MQ=18)
                                                                                       taggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg             >  1:1421353/4‑100 (MQ=18)
                                                                                        aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc            >  2:1305699/3‑100 (MQ=18)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGc            >  2:1413983/3‑100 (MQ=21)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc            >  2:181404/3‑100 (MQ=17)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  1:3132533/3‑100 (MQ=21)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  1:2382679/3‑100 (MQ=21)
                                                                                         tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa           >  2:922977/1‑100 (MQ=255)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:3274293/2‑100 (MQ=18)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:1085467/2‑100 (MQ=17)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:210517/2‑100 (MQ=21)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:2834935/2‑100 (MQ=17)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:1682563/2‑100 (MQ=17)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:2699243/2‑100 (MQ=18)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt          >  2:2446522/2‑100 (MQ=21)
                                                                                          gCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa          >  1:2144166/1‑99 (MQ=18)
                                                                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCag          >  2:2571260/1‑99 (MQ=18)
                                                                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt         >  2:3018624/1‑100 (MQ=21)
                                                                                               aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg     <  1:2500960/100‑1 (MQ=255)
                                                                                                  cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct  >  2:852676/1‑100 (MQ=255)
                                                                                                  |                                                                                                         
GGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCT  >  NC_000913/3705873‑3706070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: