Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,984 T→G 33.8% intergenic (‑194/+114) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG33.8% 283.5 / 204.2 181intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (59/61);  new base G (57/4);  total (116/65)
Fisher's exact test for biased strand distribution p-value = 4.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-02

Only 100 of 182 total aligned reads displayed.

TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTC  >  NC_000913/3705886‑3706082
                                                                                                  |                                                                                                        
 gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACACAGTGCGCTCTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                        >  1:2775941/1‑100 (MQ=255)
 gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                        >  1:1070749/1‑100 (MQ=255)
  ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                       >  2:525132/1‑100 (MQ=255)
       ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                  >  2:2292391/1‑100 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                 <  2:1348758/100‑1 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                 <  2:575896/100‑1 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                 <  2:1673983/100‑1 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                                 <  1:2577210/100‑2 (MQ=37)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                               <  2:3350030/100‑1 (MQ=255)
            gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                            >  2:1976710/1‑100 (MQ=255)
            gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaaa                                                                                             <  1:973800/100‑3 (MQ=25)
             cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                           >  2:433335/1‑100 (MQ=255)
              gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                          >  2:453470/1‑100 (MQ=255)
                tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                        <  1:481235/100‑1 (MQ=255)
                 gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                       >  2:2647779/1‑100 (MQ=255)
                    cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                    <  1:489847/100‑1 (MQ=255)
                     gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                   <  1:1576694/100‑1 (MQ=255)
                     gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                   <  2:303189/100‑1 (MQ=255)
                     gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                   <  1:1246125/100‑1 (MQ=255)
                      cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                  <  1:2412074/100‑1 (MQ=255)
                      cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                  <  2:3345516/100‑1 (MQ=255)
                         agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                               <  2:1376918/100‑1 (MQ=255)
                           gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                             <  1:708013/100‑1 (MQ=255)
                            cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                            >  1:1514883/1‑100 (MQ=255)
                            cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                            <  2:1070752/100‑1 (MQ=255)
                            cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                            >  2:1083528/1‑100 (MQ=255)
                             acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                                           >  2:652931/1‑100 (MQ=255)
                              caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                          <  1:2673634/100‑1 (MQ=255)
                              caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                          <  1:768381/100‑1 (MQ=255)
                                  gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                      >  2:2882506/1‑100 (MQ=255)
                                  gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                      <  2:2850252/100‑1 (MQ=255)
                                  cccACCGGGTATACAGATTGATGACAAAATGCGCCATGTTTATGACAGAAACACCGTTAACGACTTGTCCGTCCTACAACATAATGCAAATTCAAGACa                                                                       >  1:2873693/2‑99 (MQ=255)
                                   ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCGTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                     >  2:2377219/1‑100 (MQ=255)
                                      ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                                  <  1:1618408/100‑1 (MQ=255)
                                        gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                               >  1:417066/1‑100 (MQ=255)
                                                aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                                   <  2:92022/100‑1 (MQ=255)
                                                 gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                                  <  1:1000320/100‑1 (MQ=255)
                                                  aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                                  <  2:1621402/99‑1 (MQ=255)
                                                  aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAg                                                 >  2:2161586/1‑100 (MQ=255)
                                                   ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                                  >  1:1692304/1‑98 (MQ=255)
                                                     gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                              >  1:2594915/1‑100 (MQ=255)
                                                     gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                              >  1:1379029/1‑100 (MQ=255)
                                                      ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                             <  2:3246252/100‑1 (MQ=255)
                                                       tcaCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTg                                            >  2:2291088/3‑100 (MQ=255)
                                                        gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                             >  1:3114353/1‑98 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATa                                         <  1:3339263/100‑1 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATa                                         <  1:1185480/100‑1 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATa                                         >  2:1247314/1‑100 (MQ=255)
                                                               tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCgg                                    <  1:1464915/100‑1 (MQ=255)
                                                                gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGa                                   <  1:2525268/100‑1 (MQ=255)
                                                                 cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                                  >  2:1512176/1‑100 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTCCAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                 <  1:2167561/100‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                 <  1:156416/100‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                 <  1:859607/100‑1 (MQ=255)
                                                                     ctttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAgcgcagcgca                              >  2:3390066/9‑100 (MQ=21)
                                                                      tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                             >  1:2121174/1‑100 (MQ=255)
                                                                        tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                           <  1:2396405/100‑1 (MQ=255)
                                                                         gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                         >  2:1291721/5‑100 (MQ=18)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAg                          >  2:1571330/5‑100 (MQ=18)
                                                                          taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                         >  1:2980811/4‑100 (MQ=18)
                                                                           aggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  2:3149123/3‑100 (MQ=18)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  2:181404/3‑100 (MQ=17)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:3132533/3‑100 (MQ=21)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:2382679/3‑100 (MQ=21)
                                                                           aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                        <  2:209180/100‑1 (MQ=255)
                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  2:3029712/2‑100 (MQ=18)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  2:2699243/2‑100 (MQ=18)
                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                      >  2:879412/1‑100 (MQ=255)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                     >  2:247189/1‑100 (MQ=18)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                      >  2:1782752/1‑99 (MQ=17)
                                                                             gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCag                      >  2:2571260/1‑99 (MQ=18)
                                                                              ccggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                     >  2:546887/9‑98 (MQ=18)
                                                                              ccggataaggcgTTCACGCCTCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                     >  1:2072701/9‑98 (MQ=17)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaat                     >  2:224634/9‑98 (MQ=25)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                     >  2:781015/9‑98 (MQ=21)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                     >  2:1672967/9‑98 (MQ=18)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                   >  1:1667946/7‑100 (MQ=18)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtt                    >  1:3008101/7‑99 (MQ=21)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                   >  2:671829/7‑100 (MQ=14)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTTATAAGCGTAGCGCATCAGGCAgttt                   >  1:2621897/7‑100 (MQ=17)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattc                   >  1:2426462/7‑99 (MQ=21)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                   >  2:2622136/7‑100 (MQ=17)
                                                                                ggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaacta                   >  2:569447/7‑96 (MQ=25)
                                                                                 gataaggcgTTCACGCCTTATCCTGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATGGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttttt                  >  2:994446/6‑100 (MQ=14)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                 <  1:2500960/100‑1 (MQ=255)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                 <  1:3231696/100‑1 (MQ=255)
                                                                                   taaggcgTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAAGCAgttt                   >  2:779446/4‑97 (MQ=21)
                                                                                     cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttttttctt              >  1:3390503/1‑96 (MQ=255)
                                                                                     cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct              >  2:852676/1‑100 (MQ=255)
                                                                                      ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt             >  2:2436216/1‑100 (MQ=255)
                                                                                        cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg           <  2:318497/100‑1 (MQ=255)
                                                                                         gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc          <  2:1308958/100‑1 (MQ=255)
                                                                                          ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc         >  2:1869514/1‑100 (MQ=255)
                                                                                           tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa        >  1:1605822/1‑100 (MQ=255)
                                                                                            aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTTGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt       >  1:364156/1‑100 (MQ=255)
                                                                                             aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc      <  2:3286066/100‑1 (MQ=255)
                                                                                             aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc      <  2:1691979/100‑1 (MQ=255)
                                                                                              cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa     >  2:1964953/1‑100 (MQ=255)
                                                                                               gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg    <  2:114407/100‑1 (MQ=255)
                                                                                                ccTTATCCGGCCTACAAAATCGTACAAATTCAATACATTGCAGACTTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATGTTTGCCTTTGCCCTCAGt   >  1:3326085/1‑100 (MQ=21)
                                                                                                  |                                                                                                        
TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTC  >  NC_000913/3705886‑3706082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: