Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,985 T→C 33.7% intergenic (‑195/+113) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9850TC33.7% 255.1 / 176.3 181intergenic (‑195/+113)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (59/61);  new base C (57/4);  total (116/65)
Fisher's exact test for biased strand distribution p-value = 4.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-02

Only 100 of 183 total aligned reads displayed.

TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTT  >  NC_000913/3705886‑3706084
                                                                                                   |                                                                                                         
 gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACACAGTGCGCTCTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                          >  1:2775941/1‑100 (MQ=255)
 gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                          >  1:1070749/1‑100 (MQ=255)
  ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                         >  2:525132/1‑100 (MQ=255)
       ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                    >  2:2292391/1‑100 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                   <  2:1348758/100‑1 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                   <  2:575896/100‑1 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                   <  2:1673983/100‑1 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                                   <  1:2577210/100‑2 (MQ=37)
         aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                                  <  2:1617370/100‑3 (MQ=37)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                                 <  2:3350030/100‑1 (MQ=255)
            gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                              >  2:1976710/1‑100 (MQ=255)
            gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaaa                                                                                               <  1:973800/100‑3 (MQ=25)
             cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                             >  2:433335/1‑100 (MQ=255)
              gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                            >  2:453470/1‑100 (MQ=255)
                tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                          <  1:481235/100‑1 (MQ=255)
                 gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                         >  2:2647779/1‑100 (MQ=255)
                    cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTACCCGGCCTACAAAATCGTg                                                                                      <  1:490811/100‑1 (MQ=255)
                     gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                     <  1:1576694/100‑1 (MQ=255)
                     gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                     <  2:303189/100‑1 (MQ=255)
                     gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                     <  1:1246125/100‑1 (MQ=255)
                      cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                    <  1:2412074/100‑1 (MQ=255)
                         agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                                 <  2:1376918/100‑1 (MQ=255)
                            cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                              >  1:1514883/1‑100 (MQ=255)
                            cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                              <  2:1070752/100‑1 (MQ=255)
                             acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                                             >  2:652931/1‑100 (MQ=255)
                              caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                            <  1:2673634/100‑1 (MQ=255)
                                  gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                        >  2:2882506/1‑100 (MQ=255)
                                  gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                        <  2:2850252/100‑1 (MQ=255)
                                  gcCACCGGGTATACAGATTCTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                        <  1:360147/100‑1 (MQ=255)
                                  cccACCGGGTATACAGATTGATGACAAAATGCGCCATGTTTATGACAGAAACACCGTTAACGACTTGTCCGTCCTACAACATAATGCAAATTCAAGACa                                                                         >  1:2873693/2‑99 (MQ=255)
                                   ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCGTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                       >  2:2377219/1‑100 (MQ=255)
                                      ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                                    <  1:1618408/100‑1 (MQ=255)
                                        gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                                 >  1:417066/1‑100 (MQ=255)
                                             taCAGATTGTTCACAATGTTCGCTTTGTTTGTTCCGGATGCGGCGTTTAGCCCTTATCCGGTCTACAGTATCGTTCAATTTCATTTCATTGAAGAGttt                                                           >  1:2597208/1‑99 (MQ=12)
                                              aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTc                                                         <  2:1808234/100‑1 (MQ=255)
                                                aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                                     <  2:92022/100‑1 (MQ=255)
                                                 gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                                    <  1:1000320/100‑1 (MQ=255)
                                                  aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAg                                                   >  2:2161586/1‑100 (MQ=255)
                                                   ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                                    >  1:1692304/1‑98 (MQ=255)
                                                   ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                                  <  2:1223669/100‑1 (MQ=255)
                                                     gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                                >  1:1379029/1‑100 (MQ=255)
                                                      ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                               <  2:3246252/100‑1 (MQ=255)
                                                      ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                               <  1:2990399/100‑1 (MQ=255)
                                                        gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                               >  1:3114353/1‑98 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATa                                           <  1:73175/100‑1 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATa                                           <  1:1185480/100‑1 (MQ=255)
                                                            aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAg                                         >  2:2525288/1‑100 (MQ=255)
                                                               tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCgg                                      <  1:1464915/100‑1 (MQ=255)
                                                                gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGa                                     <  1:2525268/100‑1 (MQ=255)
                                                                 cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                                    >  2:1512176/1‑100 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTCCAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                   <  1:2167561/100‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                   <  2:1545345/100‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                   <  1:156416/100‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                   <  1:859607/100‑1 (MQ=255)
                                                                   cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                                  <  2:1265712/100‑1 (MQ=255)
                                                                   cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                                  <  2:2254074/100‑1 (MQ=255)
                                                                     ctttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAgcgcagcgca                                >  2:3390066/9‑100 (MQ=21)
                                                                      tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                               >  1:2121174/1‑100 (MQ=255)
                                                                         gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                           >  2:1291721/5‑100 (MQ=18)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAAACCTGCAAATTCAATATATTGCAGGATACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                            >  1:3358721/5‑100 (MQ=18)
                                                                           aggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                          >  2:3149123/3‑100 (MQ=18)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:2382679/3‑100 (MQ=21)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:3132533/3‑100 (MQ=21)
                                                                           aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                          <  2:209180/100‑1 (MQ=255)
                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  2:1085467/2‑100 (MQ=17)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:2398409/2‑100 (MQ=18)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTC‑‑‑‑AGGCCTGATAAGCGTAGCGCATCAGGctt                        >  2:1840620/2‑98 (MQ=21)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  2:2699243/2‑100 (MQ=18)
                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                        >  2:879412/1‑100 (MQ=255)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                       >  2:247189/1‑100 (MQ=18)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                        >  2:1782752/1‑99 (MQ=17)
                                                                             gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                       >  2:3018624/1‑100 (MQ=21)
                                                                              ccggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                       >  2:546887/9‑98 (MQ=18)
                                                                              ccggataaggcgTTCACGCCTCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                       >  1:2072701/9‑98 (MQ=17)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaat                       >  2:224570/9‑98 (MQ=25)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                       >  2:781015/9‑98 (MQ=21)
                                                                               cggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAatt                      >  2:2618651/8‑100 (MQ=17)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  2:1897287/7‑100 (MQ=18)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  1:1667946/7‑100 (MQ=18)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  2:671829/7‑100 (MQ=14)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTTATAAGCGTAGCGCATCAGGCAgttt                     >  1:2621897/7‑100 (MQ=17)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattc                     >  1:2426462/7‑99 (MQ=21)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                    >  1:3025458/7‑98 (MQ=21)
                                                                                ggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaacta                     >  2:569447/7‑96 (MQ=25)
                                                                                 gataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttt                    >  2:3318869/6‑100 (MQ=18)
                                                                                 gataaggcgTTCACGCCTTATCCTGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATGGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttttt                    >  2:994446/6‑100 (MQ=14)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                   <  1:2500960/100‑1 (MQ=255)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                   <  1:3231696/100‑1 (MQ=255)
                                                                                   taaggcgTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAAGCAgttt                     >  2:779446/4‑97 (MQ=21)
                                                                                     cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                >  2:852676/1‑100 (MQ=255)
                                                                                      ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt               >  2:2436216/1‑100 (MQ=255)
                                                                                        cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg             <  2:318497/100‑1 (MQ=255)
                                                                                         gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc            <  2:1308958/100‑1 (MQ=255)
                                                                                           tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGTCTTTGTCa          >  1:826406/1‑100 (MQ=255)
                                                                                           tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa          >  1:1605822/1‑100 (MQ=255)
                                                                                            aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTTGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt         >  1:364156/1‑100 (MQ=255)
                                                                                             aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc        <  2:1691979/100‑1 (MQ=255)
                                                                                             aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc        >  2:1653727/1‑100 (MQ=255)
                                                                                             aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc        <  2:3286066/100‑1 (MQ=255)
                                                                                                ccTTATCCGGCCTACAAAATCGTACAAATTCAATACATTGCAGACTTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATGTTTGCCTTTGCCCTCAGt     >  1:3326085/1‑100 (MQ=21)
                                                                                                   |                                                                                                         
TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTT  >  NC_000913/3705886‑3706084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: