BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,853,909	A→G	100%	intergenic (+267/‑13)	tisB → / → emrD	toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,853,909	0	A	G	100.0%	479.7 / ‑6.7	138	intergenic (+267/‑13)	tisB/emrD	toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (77/58); minor base T (1/1); total (79/59)

Only 100 of 138 total aligned reads displayed.

CCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATAT  >  NC_000913/3853814‑3854004

ccTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCAt                                                                                             <  1:406552/100‑1 (MQ=255)

 cTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATc                                                                                            >  2:1285053/1‑100 (MQ=255)

  tGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATcc                                                                                           >  2:2227549/1‑100 (MQ=255)

  tGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATcc                                                                                           >  1:290542/1‑100 (MQ=255)

    gCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGt                                                                                         <  1:3085913/100‑1 (MQ=255)

     cGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTg                                                                                        >  1:1327538/1‑100 (MQ=255)

       tataTCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGAt                                                                                      >  2:2120844/1‑100 (MQ=255)

       tataTCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGAt                                                                                      <  2:1988991/100‑1 (MQ=255)

        atatCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTATTTATTTTTATAAAGGCATCCGTGAt                                                                                      >  1:156708/1‑99 (MQ=255)

        atatCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATa                                                                                     >  1:1736804/1‑100 (MQ=255)

         tatCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATaa                                                                                    >  1:1736924/1‑100 (MQ=255)

         natCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATaa                                                                                    >  1:2544992/2‑100 (MQ=255)

          atCTGGCTAACATTCATCAATGTGATAGATTCCTTTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAAt                                                                                   <  2:1630355/100‑1 (MQ=255)

          atCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAAt                                                                                   <  1:2120829/100‑1 (MQ=255)

           tCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATg                                                                                  >  1:2002495/1‑100 (MQ=255)

            cTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGa                                                                                 >  2:2741042/1‑100 (MQ=255)

            cTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGa                                                                                 <  2:1011560/100‑1 (MQ=255)

              ggCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGaaa                                                                               >  1:2001141/1‑100 (MQ=255)

              ggCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGaaa                                                                               >  2:1303933/1‑100 (MQ=255)

                 tAACATTCATCAATGTGATAGATTCCTCTCTCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGAGATAATGAAAAgg                                                                            >  1:1126830/1‑100 (MQ=255)

                  aaCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGc                                                                           <  2:3178298/100‑1 (MQ=255)

                   aCATTCATCAATGTGATAGATTCCTCTTCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATGATTTTTATAAAGGCATCCGTGATAATGAAAAGGCa                                                                          <  2:3363650/100‑1 (MQ=255)

                   aCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCa                                                                          <  2:1290749/100‑1 (MQ=255)

                    cATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCaa                                                                         >  1:2702712/1‑100 (MQ=255)

                    cATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATAATTTTTATAAACGCATCCGTGATAATGAAAAg                                                                             >  2:417146/1‑96 (MQ=255)

                      ttCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAg                                                                       >  2:3176816/1‑100 (MQ=255)

                       tcatcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGGAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGa                                                                      >  1:2494301/1‑100 (MQ=255)

                        catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTGTTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGaa                                                                     >  1:2606610/1‑100 (MQ=255)

                        catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGaa                                                                     >  1:2709463/1‑100 (MQ=255)

                        catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGa                                                                      <  1:611071/99‑1 (MQ=255)

                          tcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAAc                                                                   >  1:944456/1‑100 (MQ=255)

                          tcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAAc                                                                   >  1:1703586/1‑100 (MQ=255)

                           caATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACg                                                                  <  2:2398380/100‑1 (MQ=255)

                             aTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTc                                                                <  1:2046639/100‑1 (MQ=255)

                               gtgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCaa                                                              >  2:1413795/1‑100 (MQ=255)

                                 gATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAtt                                                            <  2:3033941/100‑1 (MQ=255)

                                 gATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAt                                                             >  1:929252/1‑99 (MQ=255)

                                  aTAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAtt                                                            >  1:3375002/1‑99 (MQ=255)

                                   tAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTg                                                          <  1:2550016/100‑1 (MQ=255)

                                   tAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAAAGAAAAGGCAAAGAAACGTCAATTTg                                                          >  2:2923994/1‑100 (MQ=255)

                                    aGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGt                                                         <  2:1755021/100‑1 (MQ=255)

                                     gATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGtt                                                        >  1:2529197/1‑100 (MQ=255)

                                     gATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGtt                                                        >  2:2396122/1‑100 (MQ=255)

                                     gATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGtt                                                        <  2:2247393/100‑1 (MQ=255)

                                      aTTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTa                                                       <  2:515998/100‑1 (MQ=255)

                                      aTTCCTCTCACGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTa                                                       >  1:3036631/1‑100 (MQ=255)

                                       ttCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAt                                                      >  1:2187058/1‑100 (MQ=255)

                                       ttCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAt                                                      >  1:1197340/1‑100 (MQ=255)

                                        tcctccCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAt                                                      <  1:2948614/99‑1 (MQ=255)

                                        tCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAtt                                                     >  2:1387274/1‑100 (MQ=255)

                                        tCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAtt                                                     <  1:1387268/100‑1 (MQ=255)

                                         ccTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTg                                                    >  2:457907/1‑100 (MQ=255)

                                          ctctCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGa                                                   <  2:217538/100‑1 (MQ=255)

                                          ctctCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGa                                                   >  2:3292210/1‑100 (MQ=255)

                                            ctcCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATg                                                 >  2:835309/1‑100 (MQ=255)

                                             tcCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATg                                                 <  1:2363208/99‑1 (MQ=255)

                                               ccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCTAAGAAACGTCAATTTGTTATTGATGTTg                                              >  1:819527/1‑100 (MQ=255)

                                               ccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGGTATTGATGTTg                                              >  2:326416/1‑100 (MQ=255)

                                                   aTTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTAt                                          <  1:169106/100‑1 (MQ=255)

                                                   aTTTATGGGAACGCGTAGTGACCTATTCTAATTATTTTTATAAAGGCAACCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTa                                           >  1:1409514/1‑99 (MQ=255)

                                                     ttATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTa                                        >  1:2016344/1‑100 (MQ=255)

                                                     ttATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTa                                        >  1:3174357/1‑100 (MQ=255)

                                                      tATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTAc                                       >  1:3247643/1‑100 (MQ=255)

                                                      tATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTAc                                       >  2:2644370/1‑100 (MQ=255)

                                                        tGGGAATGCGTAGTGACTTATTCTAATTATTTTTTTATTTTTATCCGTGATAATGAAAAGGAAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTc                                     >  1:2449180/1‑100 (MQ=255)

                                                        tGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTc                                     >  1:3026828/1‑100 (MQ=255)

                                                        tGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTc                                     >  1:2449312/1‑100 (MQ=255)

                                                           gAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTg                                  <  2:1807440/100‑1 (MQ=255)

                                                             aTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGc                                <  2:416048/100‑1 (MQ=255)

                                                             aTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGc                                <  2:583554/100‑1 (MQ=255)

                                                               gCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCg                              <  2:2314547/100‑1 (MQ=255)

                                                                 gTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTc                            >  1:2301018/1‑100 (MQ=255)

                                                                 gTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTc                            <  2:1736935/100‑1 (MQ=255)

                                                                   aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCgg                          <  2:131579/100‑1 (MQ=255)

                                                                   aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCg                           >  2:32580/1‑99 (MQ=255)

                                                                    gTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGt                         <  1:1446300/100‑1 (MQ=255)

                                                                     tGACTTATTCTAATTATTTTTATAAAGGCATACGTGACAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTc                        <  1:2279942/100‑1 (MQ=255)

                                                                      gACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAATGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCa                       >  1:153360/1‑100 (MQ=255)

                                                                      gACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCa                       <  1:835306/100‑1 (MQ=255)

                                                                       aCTTATTCTAAGTATTTTTATAATGGCATCCGTGATAATGAAAAGGCAAAGAAGCGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCa                       <  1:3205698/99‑1 (MQ=255)

                                                                           aTTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGTCCGTCGGTCAGATgg                  >  1:2486745/1‑100 (MQ=255)

                                                                           aTTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATgg                  <  1:1637817/100‑1 (MQ=255)

                                                                           aTTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATgg                  <  2:755183/100‑1 (MQ=255)

                                                                            ttCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTTAGATGgc                 >  2:2666081/1‑100 (MQ=255)

                                                                             tCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcg                <  1:2158266/100‑1 (MQ=255)

                                                                             tCTAATTATTTGTGTAAAGGCAGCCGTGATAAGGAAGAGGCAAAGAAACGTCAATTTGTTATGGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcg                <  1:1500730/100‑1 (MQ=255)

                                                                              cTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcg                <  2:1667681/99‑1 (MQ=255)

                                                                               tAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAATAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcgc               >  1:768449/1‑99 (MQ=255)

                                                                               tAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcgc               >  1:523821/1‑99 (MQ=255)

                                                                               tAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCa              <  2:448613/100‑1 (MQ=255)

                                                                                aaTTATTTTTATAAAGGCATCCGTGATAATGAACAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCGGATGGCGCaa             >  1:2222661/1‑100 (MQ=255)

                                                                                aaTTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCaa             >  2:1495921/1‑100 (MQ=255)

                                                                                    attTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCACAcc          >  2:541636/1‑99 (MQ=255)

                                                                                     tttttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAt        >  2:2032001/1‑100 (MQ=255)

                                                                                     tttttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTAGCCGTCGGTCAGATGGCGCAAACCAt        >  2:1520716/1‑100 (MQ=255)

                                                                                      ttttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAtt       >  1:1416934/1‑100 (MQ=255)

                                                                                        ttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTta     >  1:1360404/1‑100 (MQ=255)

                                                                                        ttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTta     <  1:2785217/100‑1 (MQ=255)

                                                                                           taAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTatat  <  2:2725986/100‑1 (MQ=255)

                                                                                           taAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTatat  >  2:2196722/1‑100 (MQ=255)

CCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATAT  >  NC_000913/3853814‑3854004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`