BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	364,024	C→T	58.3%	R761H (CGC→CAC)	lacZ ←	beta‑D‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,024	0	C	T	58.3%	56.9 / 164.8	139	R761H (CGC→CAC)	lacZ	beta‑D‑galactosidase
Reads supporting (aligned to +/- strand): ref base C (30/28); new base T (47/34); total (77/62)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-03

Only 100 of 141 total aligned reads displayed.

TCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACAC  >  NC_000913/363928‑364123

tCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTggtggt                                                                                                  <  2:2001249/100‑1 (MQ=255)

tCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGt                                                                                                  <  1:3075837/100‑1 (MQ=255)

 cGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGtt                                                                                                 >  2:1847454/1‑100 (MQ=255)

  gTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTa                                                                                                <  2:2732394/100‑1 (MQ=255)

    tATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTaga                                                                                              >  2:2828645/1‑98 (MQ=255)

      tCCAGCGGTGCACGGTTGAACTGATCGCGCGGCGCCGTCCGCAGTTGTTTTTGATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAtt                                                                                            <  1:2742379/100‑1 (MQ=255)

      tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAtt                                                                                            >  1:237828/1‑100 (MQ=255)

      tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAAtt                                                                                            >  1:3141592/1‑100 (MQ=255)

      tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAAtt                                                                                            <  2:1511504/100‑1 (MQ=255)

      tCCAGCGGCGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAtt                                                                                            >  1:3291044/1‑100 (MQ=255)

        cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                          <  1:1653777/100‑1 (MQ=255)

        cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTg                                                                                           <  1:451861/99‑1 (MQ=255)

              tGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGc                                                                                    <  2:2116967/100‑1 (MQ=255)

              tGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGc                                                                                    <  1:336375/100‑1 (MQ=255)

              tGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGc                                                                                    >  2:1655943/1‑100 (MQ=255)

               gCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCt                                                                                   >  1:3023009/1‑100 (MQ=255)

                cACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCtt                                                                                  >  2:1800994/1‑100 (MQ=255)

                 aCGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCtta                                                                                 <  2:860675/100‑1 (MQ=255)

                  cGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCttat                                                                                <  2:2837758/100‑1 (MQ=255)

                    ggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGGAAGGCAGCTTGACTGGTGGTTAAATTGCCAACGCttat                                                                                >  1:2056410/1‑98 (MQ=255)

                      tGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCCACGCTTATTAcc                                                                            >  2:1674531/1‑100 (MQ=255)

                       gAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAccc                                                                           >  2:1555773/1‑100 (MQ=255)

                       gAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTAccc                                                                           <  1:545432/100‑1 (MQ=255)

                         aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAg                                                                         >  2:875601/1‑100 (MQ=255)

                         aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAg                                                                         <  1:3307409/100‑1 (MQ=255)

                          cTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGc                                                                        >  1:2898449/1‑100 (MQ=255)

                          cTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGc                                                                        >  2:2441487/1‑100 (MQ=255)

                          cTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGc                                                                        >  1:1569973/1‑100 (MQ=255)

                            naTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                      >  1:2622598/2‑100 (MQ=255)

                            gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                      >  2:3041121/1‑100 (MQ=255)

                            gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAACCCCCATCTGGGAAAGAAAGCCTGACTGGTGGCTAAATTCCCAACCCTTATTAccc                                                                           >  1:2903319/1‑95 (MQ=255)

                               cgcgCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGAt                                                                   <  2:270568/100‑1 (MQ=255)

                               cgcgCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGAt                                                                   >  1:2767898/1‑100 (MQ=255)

                                gcgcAGCGGCGTCAGCTGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATg                                                                  <  1:1978361/100‑1 (MQ=255)

                                gcgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATg                                                                  >  2:2185226/1‑100 (MQ=255)

                                gcgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATg                                                                  <  2:1873758/100‑1 (MQ=255)

                                gcgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATg                                                                  <  1:3203734/100‑1 (MQ=255)

                                 cgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGc                                                                 <  1:814490/100‑1 (MQ=255)

                                   cAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCaa                                                               >  1:505534/1‑100 (MQ=255)

                                       ggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAAt                                                           >  1:1195357/1‑100 (MQ=255)

                                            cagcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAtt                                                      >  2:426650/1‑100 (MQ=255)

                                            cagcagTTGTTTTTTATAGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAt                                                       >  2:2326238/1‑99 (MQ=255)

                                             agcagTTGTTTTTTTTTTTCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt                                                     >  1:937743/1‑100 (MQ=255)

                                              gcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTc                                                    >  1:1353636/1‑100 (MQ=255)

                                              gcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTc                                                    >  1:1579704/1‑100 (MQ=255)

                                              gcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTc                                                    >  1:902997/1‑100 (MQ=255)

                                                agTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGc                                                  >  1:256288/1‑100 (MQ=255)

                                                 gttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCt                                                 >  1:1489832/1‑100 (MQ=255)

                                                 gttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCt                                                 >  2:3258657/1‑100 (MQ=255)

                                                 gttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCt                                                 >  1:296196/1‑100 (MQ=255)

                                                 gttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCt                                                 <  2:527285/100‑1 (MQ=255)

                                                   tgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtgg                                               >  2:2783793/1‑100 (MQ=255)

                                                    gttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggt                                              >  1:952307/1‑100 (MQ=255)

                                                    gtgttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggt                                              <  1:1315160/97‑1 (MQ=255)

                                                      tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                            <  2:2526646/100‑1 (MQ=255)

                                                      tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                            <  2:1164761/100‑1 (MQ=255)

                                                      tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                            <  1:2813028/100‑1 (MQ=255)

                                                      tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                            >  2:621753/1‑100 (MQ=255)

                                                      tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                            <  1:1060177/100‑1 (MQ=255)

                                                      tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                            <  1:70474/100‑1 (MQ=255)

                                                       ttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTggtggt                                           >  2:706097/1‑100 (MQ=255)

                                                       ttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                            <  1:1496496/99‑1 (MQ=255)

                                                        tttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTc                                          <  2:610139/100‑1 (MQ=255)

                                                        tgtATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAAAAAAAAAAAAAAATCCATTTCGCTggtggt                                           <  1:1689641/97‑1 (MQ=255)

                                                         ttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCa                                         <  2:2377348/100‑1 (MQ=255)

                                                          tATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAg                                        <  2:560276/100‑1 (MQ=255)

                                                          tATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAg                                        >  1:713351/1‑100 (MQ=255)

                                                           aTCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGa                                       >  2:1695306/1‑100 (MQ=255)

                                                           aTCGCCAAACAAAATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAAAAAAAAAAAAAAAAAAATCCATTTCGCTGGTGGTCAGa                                       <  1:230132/100‑1 (MQ=255)

                                                             cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATg                                     >  1:1163149/1‑100 (MQ=255)

                                                             cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATg                                     >  2:2125397/1‑100 (MQ=255)

                                                             cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCAATTTCGCTGGTGGTCAGATg                                     >  1:2351408/1‑100 (MQ=255)

                                                             cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATg                                     <  2:610996/100‑1 (MQ=255)

                                                              gCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGc                                    >  2:2713339/1‑100 (MQ=255)

                                                              gCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGc                                    >  2:2136633/1‑100 (MQ=255)

                                                               ccAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCg                                   >  2:3106259/1‑100 (MQ=255)

                                                                cAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCgg                                  <  1:564898/100‑1 (MQ=255)

                                                                  aTCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGa                                >  2:1301147/1‑100 (MQ=255)

                                                                    ccACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATg                              >  1:146793/1‑100 (MQ=255)

                                                                    ccACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATg                              >  1:806977/1‑100 (MQ=255)

                                                                     cacaTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATgg                             >  2:2490341/1‑100 (MQ=255)

                                                                       caTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCg                           >  1:2275984/1‑100 (MQ=255)

                                                                         tCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCACGATGAAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTg                         <  1:2960978/100‑1 (MQ=255)

                                                                         tCTGTGAAAGAAAGCCTGACTGGTGATTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTg                         <  2:1080778/100‑1 (MQ=255)

                                                                          cTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTgg                        <  2:2014599/100‑1 (MQ=255)

                                                                          cTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTgg                        <  2:1074251/100‑1 (MQ=255)

                                                                           tgtgAAAGAAAGTCTGACTGGTGGTTAAATTGTCAATGCTTATTACCCTGCTCGTTGCAAAATTCCATTTCGCTGGTTGTCAGATGCGGGATGGCGTggg                       <  1:1084702/100‑1 (MQ=255)

                                                                           tgtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGAtagatcgga                       >  1:2591818/1‑92 (MQ=255)

                                                                            gtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGTGTGGGa                      >  2:683382/1‑100 (MQ=255)

                                                                            gtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGa                      <  2:2897157/100‑1 (MQ=255)

                                                                             tgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAc                     <  2:2536910/100‑1 (MQ=255)

                                                                              gaaagaaaGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTTGTGGTCAGATGCGGGATGGCGTGGGGcg                    >  1:2528495/1‑100 (MQ=255)

                                                                               aaagaaagCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgc                   >  2:125666/1‑100 (MQ=255)

                                                                                aagaaagCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgcg                  >  1:1842561/1‑100 (MQ=255)

                                                                                     agCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCggg             <  1:2776198/100‑1 (MQ=255)

                                                                                      gCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCgggg            <  1:1986815/100‑1 (MQ=255)

                                                                                      gCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCgggg            >  1:510304/1‑100 (MQ=255)

                                                                                       ccTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGa           <  1:57863/100‑1 (MQ=255)

                                                                                          gACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg        <  2:3028286/100‑1 (MQ=255)

                                                                                                cGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCacac  <  1:1112256/100‑1 (MQ=255)

TCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACAC  >  NC_000913/363928‑364123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`