Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,950,468 G→T 100% intergenic (+48/‑39) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,4680GT93.3% 317.5 / 11.1 111intergenic (+48/‑39)ilvL/ilvXilvG operon leader peptide/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (48/54);  total (53/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

Only 100 of 115 total aligned reads displayed.

TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTA  >  NC_000913/3950371‑3950560
                                                                                                   |                                                                                            
tgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGttt                                                                                            >  2:2968020/1‑100 (MQ=255)
 gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTCACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttt                                                                                           >  2:1405556/1‑100 (MQ=255)
 gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttt                                                                                           <  2:662167/100‑1 (MQ=255)
   ttattaTCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTACGGGGCtttt                                                                                          >  2:2482748/1‑99 (MQ=255)
    tattatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTtttttt                                                                                        <  2:595587/100‑1 (MQ=255)
    tattatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑‑GGGtttttttt                                                                                      <  2:1148548/100‑1 (MQ=255)
      ttatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTg                                                                                     <  2:2460014/100‑1 (MQ=255)
       tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTg                                                                                     <  1:2409390/100‑1 (MQ=255)
        ataccACGGTGCGGGGCTGCACTTGGACGAGGAACGGCTTAGAGATCAAGGCTTAACTAACTAAGCCCCCCGCGCAGAAAGGTCCGCGGTTTTTTCTTGa                                                                                    <  1:1474803/97‑1 (MQ=255)
        atCCCACCGTGCGGGGCTGCACTTAGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGa                                                                                    <  1:300763/100‑1 (MQ=255)
         tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAc                                                                                   <  2:1189569/100‑1 (MQ=255)
         tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAc                                                                                   >  1:2359614/1‑100 (MQ=255)
         tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAc                                                                                   >  2:371941/1‑100 (MQ=255)
         tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAc                                                                                   >  2:3012426/1‑100 (MQ=255)
          cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAcc                                                                                  <  2:1433289/100‑1 (MQ=255)
           ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt                                                                                 >  2:1850067/1‑100 (MQ=255)
           ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt                                                                                 >  2:3005472/1‑100 (MQ=255)
            cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCtt                                                                                >  1:1082756/1‑100 (MQ=255)
            cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCtt                                                                                <  1:1674943/100‑1 (MQ=255)
             aCCGTGCTGGGCTGCACTTGGCCGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAATGTCCGGGGTTTTTTTTTGACCt                                                                                 <  1:2219384/98‑1 (MQ=255)
             aCCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTa                                                                               >  2:1392076/1‑100 (MQ=255)
             aCCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTa                                                                               <  2:2540813/100‑1 (MQ=255)
                 tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaaa                                                                           <  2:2134888/100‑1 (MQ=255)
                  gCGGGGTTGCACTTGGACGGGGAAGGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTaaaaa                                                                           <  2:1603634/99‑1 (MQ=255)
                  gCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAAc                                                                          <  2:1626563/100‑1 (MQ=255)
                   cGGGGCTGCACTTGGACGAGAAAAGGAATAGAGATCAAGCCTTAACGAACTAAAACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaaa                                                                           >  1:652742/1‑98 (MQ=255)
                     gggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATa                                                                       >  2:491403/1‑100 (MQ=255)
                       gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATaa                                                                      >  1:1740829/1‑99 (MQ=255)
                       gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAAc                                                                     <  1:1596425/100‑1 (MQ=255)
                        cTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAcc                                                                    >  2:502529/1‑100 (MQ=255)
                         tGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCg                                                                   <  2:3065407/100‑1 (MQ=255)
                         tGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCg                                                                   >  1:942250/1‑100 (MQ=255)
                          gCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCga                                                                  >  2:2448225/1‑100 (MQ=255)
                            aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCgagg                                                                >  1:3153128/1‑100 (MQ=255)
                             cTTGGACGAGGAAAGGCGTAGAGATCAAGCCTTAACGAACGAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCgagn                                                                <  2:845411/99‑2 (MQ=255)
                               tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                             <  2:2709603/100‑1 (MQ=255)
                               tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                             <  2:2049622/100‑1 (MQ=255)
                                 gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg                                                           >  2:895938/1‑100 (MQ=255)
                                 gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg                                                           >  2:1613200/1‑100 (MQ=255)
                                  aggAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                          <  2:335584/98‑1 (MQ=255)
                                  aCGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                          <  2:888713/100‑1 (MQ=255)
                                      ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                      >  2:2845314/1‑100 (MQ=255)
                                      ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                      >  2:844662/1‑100 (MQ=255)
                                      ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                      <  2:2911954/100‑1 (MQ=255)
                                       ggaaGGCTTCGAGAGCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATg                                                     <  2:133016/98‑1 (MQ=255)
                                       gAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATg                                                     <  2:1766529/100‑1 (MQ=255)
                                        aaaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGa                                                    >  2:3351037/1‑100 (MQ=255)
                                           ggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACTATGAAt                                                  >  1:182996/1‑99 (MQ=255)
                                           ggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGaa                                                   >  1:1699905/1‑98 (MQ=255)
                                           ggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                 <  1:1127551/100‑1 (MQ=255)
                                            gCTGAGAGATCATGCCTTAACGAACTAGGACCCCCGCACCGAATGGGCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                 <  1:2958836/99‑1 (MQ=255)
                                             cgtAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                               <  2:262345/98‑1 (MQ=255)
                                             cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                               <  2:2450674/100‑1 (MQ=255)
                                              ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAACGAGGAGCAGACAATGAATAAc                                               >  1:800963/1‑99 (MQ=255)
                                                agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg                                             >  1:1178778/1‑99 (MQ=255)
                                                  agaTCAAGCCTTAACGAACTAAGGCCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcac                                          <  2:3168544/100‑1 (MQ=255)
                                                   gaTCAAGACTTAACGAACTAAGACCCACGCACCGAAAGGTCCGAAGTTGTCTTTTGACCTTAAAAACATAACCGAGTAGTAGACAATGAATAACAGctta                                         <  1:3171591/100‑4 (MQ=255)
                                                   cttCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcaca                                         <  2:2620721/98‑1 (MQ=255)
                                                      cAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaaa                                      >  2:53643/1‑100 (MQ=255)
                                                      cAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaaa                                      >  2:1997280/1‑100 (MQ=255)
                                                         gCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACCGCACAAAATTc                                   >  2:710079/1‑100 (MQ=255)
                                                         gCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                                   >  2:3246307/1‑100 (MQ=255)
                                                         gCCTTAACGAACCAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                                   <  2:21107/100‑1 (MQ=255)
                                                          ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt                                  >  2:3282265/1‑100 (MQ=255)
                                                           cTTAACGACCGAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt                                  <  2:1634627/99‑1 (MQ=255)
                                                           cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTTAAAACATAACCGCGGAGCAGACAATGAATAACAGCACAAAAtt                                    >  1:1801447/1‑96 (MQ=255)
                                                            ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                                 >  2:2056152/1‑99 (MQ=255)
                                                            ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt                                >  2:1708513/1‑100 (MQ=255)
                                                            ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTCTTTTTTTGACCTTAAAAACTTAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt                                <  1:1841094/100‑1 (MQ=255)
                                                            gaaactaactAAGCCCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt                                <  2:2644862/98‑1 (MQ=255)
                                                             tAACGATCTAAGACCCCCGAACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt                                <  1:727755/99‑1 (MQ=255)
                                                               aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTGGTTTTTTTGACCTTAAAAAAATAACCGATGGGGAGACAATGAATAACTGCACAAAATTCTGtt                               >  1:1656654/1‑99 (MQ=255)
                                                               aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                             <  1:1118156/100‑1 (MQ=255)
                                                               aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                             >  2:2181933/1‑100 (MQ=255)
                                                                tgAATTAAGGCCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                             <  1:764308/98‑1 (MQ=255)
                                                                cGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct                            >  2:2184030/1‑100 (MQ=255)
                                                                cGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGATCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct                            >  1:1912429/1‑100 (MQ=255)
                                                                 gAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtctc                           >  2:316607/1‑100 (MQ=255)
                                                                  aaCTTAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                             <  1:1117059/97‑1 (MQ=255)
                                                                   aCTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa                         >  1:2898225/1‑100 (MQ=255)
                                                                    cTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTaaa                         >  1:1470076/1‑99 (MQ=255)
                                                                    cTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAg                        <  1:39200/100‑1 (MQ=255)
                                                                     tgtgttccccGTACCGACAGGTCCGGGTTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct                            <  1:2040269/90‑1 (MQ=255)
                                                                     tAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGa                       >  2:2595936/1‑100 (MQ=255)
                                                                     gaaGACCCCCCCCCCAAAGGGTCGGGGGTTTTTTTTTGACATCAAAAACATAACCAAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGa                       <  2:3199617/99‑1 (MQ=255)
                                                                          cccccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg                  <  1:1110126/100‑1 (MQ=255)
                                                                           ccccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg                 <  2:919527/100‑1 (MQ=255)
                                                                            cccGCACCGAAAGGTCCGGGGTTTTTTTTTTTTTTTTTTTACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg                 >  1:2720088/1‑99 (MQ=255)
                                                                            cccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGa                <  1:2398436/100‑1 (MQ=255)
                                                                            cccGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg                  >  1:338768/1‑97 (MQ=255)
                                                                            cccGAACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg                  <  1:120774/98‑1 (MQ=255)
                                                                                cACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgggg           <  1:2582473/100‑1 (MQ=255)
                                                                                  ccGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa          >  2:890204/1‑100 (MQ=255)
                                                                                  ccGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa          <  2:2528656/100‑1 (MQ=255)
                                                                                    gAATGGTCCGGGGTGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa                   <  1:1866541/89‑1 (MQ=255)
                                                                                      aaGGTCCGGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaact       <  1:2229990/100‑1 (MQ=255)
                                                                                      aaGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaacta      <  2:1912442/100‑1 (MQ=255)
                                                                                      aaGGGCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa          <  1:1449860/96‑1 (MQ=255)
                                                                                         gTCCGGGGTTTTTTTTTTTTTTTTATAACATAAACACGGCGCACACCATGGATAACAGCACAAAATTCGTGTTCTCACGCTTCCGGACGGGGaactagct   >  1:501453/1‑100 (MQ=255)
                                                                                          tCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACCGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAactaacta  >  2:1974507/1‑100 (MQ=255)
                                                                                                   |                                                                                            
TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTA  >  NC_000913/3950371‑3950560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: