Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,015,333 A→T 17.7% intergenic (+19/+21) metE → / ← ysgA 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,015,3330AT17.7% 387.1 / 76.4 164intergenic (+19/+21)metE/ysgA5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase
Reads supporting (aligned to +/- strand):  ref base A (55/79);  new base T (11/18);  total (67/97)
Fisher's exact test for biased strand distribution p-value = 8.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

Only 100 of 169 total aligned reads displayed.

CTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGA  >  NC_000913/4015234‑4015432
                                                                                                   |                                                                                                   
cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTaa                                                                                                     >  2:1855096/1‑100 (MQ=255)
 tGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAAt                                                                                                    >  1:2849792/1‑100 (MQ=255)
    aaaCGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATacc                                                                                                 >  2:547528/1‑100 (MQ=255)
     taCGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATacc                                                                                                 <  1:2051884/98‑1 (MQ=255)
       cgcgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATaccacc                                                                                              >  1:2533578/1‑100 (MQ=255)
       cgcgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATaccac                                                                                               <  1:626893/99‑1 (MQ=255)
        gcgcgGCTGGCCAGAACCCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTACTACCAccc                                                                                             >  2:3164959/1‑100 (MQ=255)
        gcgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCAccc                                                                                             <  1:547525/100‑1 (MQ=255)
         cgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAGATCCAAACCGGGGGGTAATaccac                                                                                               >  1:1223497/1‑97 (MQ=255)
         cgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCg                                                                                            >  1:1963064/1‑100 (MQ=255)
          gcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCgg                                                                                           <  1:1675763/100‑1 (MQ=255)
          gcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCgg                                                                                           <  2:1592614/100‑1 (MQ=255)
          gcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCgg                                                                                           <  1:1019279/100‑1 (MQ=255)
          gcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCgg                                                                                           >  1:620658/1‑100 (MQ=255)
           cgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGt                                                                                          <  2:38392/100‑1 (MQ=255)
           cgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGt                                                                                          <  2:2476840/100‑1 (MQ=255)
             gCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGttt                                                                                        <  1:1352620/100‑3 (MQ=255)
             gCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCt                                                                                        >  2:1050766/1‑100 (MQ=255)
              cTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGtttg                                                                                       <  1:3229340/100‑4 (MQ=255)
              cTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGtttg                                                                                       <  2:2261633/100‑4 (MQ=255)
               tGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCttt                                                                                      <  1:1253559/100‑1 (MQ=255)
                ggCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCtttt                                                                                     >  1:261126/1‑100 (MQ=255)
                  ccAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTtct                                                                                   <  2:1536966/100‑1 (MQ=255)
                   cAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGtttggattt                                                                                  <  2:3075495/100‑9 (MQ=38)
                    aGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCa                                                                                 <  2:1512361/100‑1 (MQ=255)
                    aGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCa                                                                                 <  2:1022424/100‑1 (MQ=255)
                     gAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCAt                                                                                <  2:3302502/100‑1 (MQ=255)
                     gAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCAt                                                                                >  2:696763/1‑100 (MQ=255)
                       aaCCCGCGCGGCACTGACGAATATGGTGCAGGCGGCGCAGAACTTGCTTCGGGGGTAAAATCCAAACCGCGTGGTAATACCACCCGGTCTTTTCTCATTa                                                                              >  1:512466/1‑100 (MQ=255)
                         cccGTGCGGCACTGGCGAACATGGTGCAGGCGGCGCGGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACa                                                                            <  1:2350626/100‑1 (MQ=255)
                         cccGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACa                                                                            <  1:3159854/100‑1 (MQ=255)
                         cccGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACa                                                                            <  2:516612/100‑1 (MQ=255)
                          ccGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCCCCCGGTCTTTTCTCATTACAg                                                                           <  1:2517348/100‑1 (MQ=255)
                            gcgcgGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCg                                                                         <  1:2919155/100‑1 (MQ=255)
                              gcgGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGAc                                                                       >  2:719370/1‑100 (MQ=255)
                                ggCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGActt                                                                     >  1:1589523/1‑100 (MQ=255)
                                 gCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGActtc                                                                    >  2:2957665/1‑100 (MQ=255)
                                  cACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGActtct                                                                   <  1:638283/100‑1 (MQ=255)
                                    cTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACttcttc                                                                 >  2:136100/1‑100 (MQ=255)
                                    cTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACttcttc                                                                 <  2:286195/100‑1 (MQ=255)
                                    cTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACttcttc                                                                 >  2:2694422/1‑100 (MQ=255)
                                     tGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTcc                                                                <  2:1273756/100‑1 (MQ=255)
                                      ggCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTccc                                                               <  1:99087/100‑1 (MQ=255)
                                       gCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCcca                                                              >  2:1737019/1‑100 (MQ=255)
                                       gCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCcca                                                              <  1:2645116/100‑1 (MQ=255)
                                       gCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCcca                                                              <  1:1475055/100‑1 (MQ=255)
                                             aTGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATAc                                                        <  2:2199897/100‑1 (MQ=255)
                                              tGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACt                                                       >  2:2680091/1‑100 (MQ=255)
                                               ggTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTg                                                      >  2:976363/1‑100 (MQ=255)
                                                gTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATATCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGc                                                     <  2:1954289/100‑1 (MQ=255)
                                                gTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGc                                                     <  2:620661/100‑1 (MQ=255)
                                                 tGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCt                                                    <  2:1340133/100‑1 (MQ=255)
                                                   cAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTa                                                  <  1:2253994/100‑1 (MQ=255)
                                                     ggcggcGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTaaa                                                >  1:1154278/1‑100 (MQ=255)
                                                     ggcggcGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTaaa                                                >  2:1591631/1‑100 (MQ=255)
                                                      gcggcgCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAc                                               >  1:2784760/1‑100 (MQ=255)
                                                      gcggcgCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAc                                               <  2:2059280/100‑1 (MQ=255)
                                                      gcggcgCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAc                                               <  2:625726/100‑1 (MQ=255)
                                                      gcggcgCAGAACCTGCGTCGGGGGTAACATCCAAACCGGGTGGTAATACCACCCGGTCTGTTCTCATCACAGCGACTTCTTCCCACCATACTGCTTaagc                                               <  1:1608964/100‑3 (MQ=255)
                                                       cggcgCAGAACTTGCGTCGGGGGTAAAATCCAACCCGGGTGCTAATACCCCACGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                              <  1:3379806/100‑1 (MQ=255)
                                                       cggcgCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                              <  1:913516/100‑1 (MQ=255)
                                                       cggcgCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                              <  1:1737008/100‑1 (MQ=255)
                                                        ggcgCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGGGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCa                                             >  2:1170488/1‑100 (MQ=255)
                                                          cgcAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                              >  1:156627/1‑97 (MQ=255)
                                                            cAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTcc                                         <  2:1787447/100‑1 (MQ=255)
                                                            cAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTCCTTCCCACCATACTGCTTAAACCATTcc                                         <  2:1391147/100‑1 (MQ=255)
                                                              gAACTTGCGTCGGGGGTAAAATCCAAACCGGGGGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTCAACCATTCCAg                                       >  1:2890593/1‑100 (MQ=255)
                                                                aCTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCa                                     <  1:3080496/100‑1 (MQ=255)
                                                                aCTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCa                                     <  1:680540/100‑1 (MQ=255)
                                                                 cTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCAt                                    <  2:1005163/100‑1 (MQ=255)
                                                                 cTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCAt                                    >  2:1882959/1‑100 (MQ=255)
                                                                   tGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATAc                                  <  2:2801607/100‑1 (MQ=255)
                                                                     cGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGc                                >  1:650431/1‑100 (MQ=255)
                                                                     cGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGc                                <  1:1914181/100‑1 (MQ=255)
                                                                      gTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCt                               >  2:119122/1‑100 (MQ=255)
                                                                          ggggTAAAATCCAAACCGGGGGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgcca                           >  2:1448928/1‑100 (MQ=255)
                                                                           gggTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgccag                          >  1:206311/1‑100 (MQ=255)
                                                                               tgagaaaagaCCGGGTGGTATTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTCCCACCCAt                      >  1:2658365/10‑100 (MQ=37)
                                                                               aaaaTCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCGTATGCTggaatggtt                      <  1:3115214/100‑9 (MQ=255)
                                                                               aaaaTCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAt                      <  1:530535/100‑1 (MQ=255)
                                                                                aaaTCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAtc                     >  2:2339060/1‑100 (MQ=255)
                                                                                 aaTCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAtct                    >  2:441156/1‑100 (MQ=255)
                                                                                     cAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTg                >  2:1610521/1‑100 (MQ=255)
                                                                                     aagaCCGGGTGGTATTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTTCTTAAACCATTCCAGCATACGCCGCCAGCCAtctt                   >  1:3360039/4‑97 (MQ=39)
                                                                                       gaCCGGGTGGTATTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCa              >  1:882763/2‑100 (MQ=255)
                                                                                        aCCGGGTGGTATTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAg             >  1:347997/1‑100 (MQ=255)
                                                                                        aCCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAg             <  1:1344068/100‑1 (MQ=255)
                                                                                         ccGGGTGGTATTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAGCATTCAGGCATCCGCCGCCAGCCATCTTCTGCAg             >  1:3084794/1‑99 (MQ=255)
                                                                                         ccGGGTGGTATTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGa            >  2:2788877/1‑100 (MQ=255)
                                                                                         ccGGGTGGTATTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGa            >  2:726165/1‑100 (MQ=255)
                                                                                         ccGGGGGGTATTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGa            >  2:1484689/1‑100 (MQ=255)
                                                                                            ggtggtATTACCACCCGGTTTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGc         >  2:306406/1‑100 (MQ=255)
                                                                                            ggtggtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGc         >  1:380698/1‑100 (MQ=255)
                                                                                             gtggtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCg        <  2:85373/100‑1 (MQ=255)
                                                                                               ngtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATAAGCTGCCAGCCATCTTCTGCAGATGCGGc      >  1:1031707/2‑100 (MQ=255)
                                                                                               ggtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGc      <  1:1316744/100‑1 (MQ=255)
                                                                                                gtAATACCACCCGGTCTTTTCTCATTACCGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCa     <  2:2729689/100‑1 (MQ=255)
                                                                                                gtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCa     <  1:710670/100‑1 (MQ=255)
                                                                                                  aaTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATg   <  1:960565/100‑1 (MQ=255)
                                                                                                   aTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGa  <  2:3311746/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGA  >  NC_000913/4015234‑4015432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: