Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 367,830 G→A 59.8% Y197Y (TAC→TAT mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913367,8300GA59.8% 107.7 / 193.8 174Y197Y (TAC→TATmhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (44/26);  new base A (56/48);  total (100/74)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 175 total aligned reads displayed.

CACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAT  >  NC_000913/367731‑367928
                                                                                                   |                                                                                                  
cacCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCg                                                                                                    <  2:803824/100‑1 (MQ=255)
  ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTa                                                                                                  <  2:1114975/100‑1 (MQ=255)
   cAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTaa                                                                                                 <  2:2909674/100‑1 (MQ=255)
       ttCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCct                                                                                             >  1:37987/1‑100 (MQ=255)
       ttCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCct                                                                                             >  2:56636/1‑100 (MQ=255)
       ttCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCct                                                                                             >  1:330946/1‑100 (MQ=255)
        tCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCctc                                                                                            <  2:2075403/100‑1 (MQ=255)
        tCAGACAGCCAATCACCCGTTGTCCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCctc                                                                                            <  1:1145366/100‑1 (MQ=255)
         cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCctct                                                                                           >  1:780209/1‑100 (MQ=255)
         cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCctct                                                                                           >  2:929719/1‑100 (MQ=255)
           gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTGCTCCTTATACCAGCCGCGGTAGTTCTGTCCGTAACCCTCttt                                                                                         >  1:1593194/1‑100 (MQ=255)
           gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCttt                                                                                         >  2:2356370/1‑100 (MQ=255)
            aCAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTg                                                                                        <  2:103815/100‑1 (MQ=255)
             cAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGCTCTTCTCATCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgc                                                                                       >  1:2176442/1‑100 (MQ=255)
             cAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgc                                                                                       >  1:895346/1‑100 (MQ=255)
              aGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcg                                                                                      >  1:840774/1‑100 (MQ=255)
               gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgc                                                                                     >  1:182047/1‑100 (MQ=255)
               gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgc                                                                                     >  1:1482585/1‑100 (MQ=255)
                ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCACAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcg                                                                                    <  1:1807855/100‑1 (MQ=255)
                 cAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgc                                                                                   >  1:2074663/1‑100 (MQ=255)
                  aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCAAAACCCTCTTTgcgcgcg                                                                                  <  1:2691235/100‑1 (MQ=255)
                   aTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgc                                                                                 <  2:218950/100‑1 (MQ=255)
                   aTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgc                                                                                 >  1:2902595/1‑100 (MQ=255)
                   aTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcg                                                                                  <  1:1611339/99‑1 (MQ=255)
                   aTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgc                                                                                 >  2:728467/1‑100 (MQ=255)
                    tCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcg                                                                                >  1:2994943/1‑100 (MQ=255)
                      aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgcg                                                                              >  2:3027073/1‑100 (MQ=255)
                      aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgcg                                                                              >  1:3359505/1‑100 (MQ=255)
                      aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcg                                                                                >  1:1367448/1‑98 (MQ=255)
                       cccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcgcgc                                                                             >  2:2789545/1‑100 (MQ=255)
                       cccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCAGTAACCCTATTTgcgcgcgcgcgc                                                                             >  1:1370024/1‑100 (MQ=255)
                          gttgttCACTGCGCAGCGGTACGGCGATAGAGGCGATTTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAg                                                                          >  2:1798441/1‑100 (MQ=255)
                          gttgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAg                                                                          >  2:2353940/1‑100 (MQ=255)
                            tgttCACTGCGCAGCGGTCCGGCGATAGAGGCGATCTTCTCCTCCTGACCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCa                                                                           >  1:3036508/1‑97 (MQ=255)
                            tgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGaa                                                                        >  1:2829624/1‑100 (MQ=255)
                             gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAAt                                                                       >  2:2618297/1‑100 (MQ=255)
                               tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATgg                                                                     >  2:1229926/1‑100 (MQ=255)
                                  cTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCt                                                                   <  2:3283167/99‑1 (MQ=255)
                                  cTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCtt                                                                  <  2:2539015/100‑1 (MQ=255)
                                   tGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTc                                                                 >  1:3300583/1‑100 (MQ=255)
                                    gcgcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTcc                                                                <  2:1827477/100‑1 (MQ=255)
                                    gcgcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTcc                                                                >  2:1060841/1‑100 (MQ=255)
                                          cGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCttt                                                          <  2:2309952/100‑1 (MQ=255)
                                           ggTACGGCGATAGAGGGGAACAACTCCTCCTGATTCCAGCCGCGGTGGTTCTGTCCGTAACTCTCTTTGCGCACGCGCGCCAGAATGGCTTCCAGCTTTa                                                         >  1:2363269/1‑100 (MQ=255)
                                           ggTACGGCGATAGAGGCGCTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTa                                                         >  2:1595881/1‑100 (MQ=255)
                                           ggTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTa                                                         <  1:1940622/100‑1 (MQ=255)
                                           ggTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTa                                                         >  2:3005172/1‑100 (MQ=255)
                                            gTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTaa                                                        >  2:2958791/1‑100 (MQ=255)
                                             tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                       <  1:1550892/100‑1 (MQ=255)
                                               cggggATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                     <  2:2138797/96‑1 (MQ=255)
                                               cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                     <  1:2847184/100‑1 (MQ=255)
                                               cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                     <  2:41631/100‑1 (MQ=255)
                                               cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                     <  2:1235696/100‑1 (MQ=255)
                                                ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGt                                                    >  1:1207515/1‑100 (MQ=255)
                                                  cGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTc                                                  <  2:3332802/100‑1 (MQ=255)
                                                   gATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTcc                                                 <  1:929716/100‑1 (MQ=255)
                                                    aTAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTccc                                                <  1:2655839/100‑1 (MQ=255)
                                                       gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                             >  2:910836/1‑100 (MQ=255)
                                                       gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                             <  1:2632161/100‑1 (MQ=255)
                                                        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                            >  2:2434076/1‑100 (MQ=255)
                                                        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                            >  2:1877247/1‑100 (MQ=255)
                                                         ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                           >  2:674265/1‑100 (MQ=255)
                                                           cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                         <  2:2777538/100‑1 (MQ=255)
                                                             aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                       <  1:1014526/100‑1 (MQ=255)
                                                             aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                       <  1:276383/100‑1 (MQ=255)
                                                             aTCTTCTCCTCCTGACCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                       <  2:1786830/100‑1 (MQ=255)
                                                              tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTg                                      >  1:3234312/1‑100 (MQ=255)
                                                                 tctcCTCCTGATCCCAGCCGCGGTGGTTCTGTCCGTAACCCTCTTCGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTCACGGTTCCCGTGCCAGTTg                                      >  1:751296/1‑97 (MQ=255)
                                                                 tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                   >  2:1771358/1‑100 (MQ=255)
                                                                 tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                   >  2:739318/1‑100 (MQ=255)
                                                                 tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                   >  2:1659107/1‑100 (MQ=255)
                                                                  ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAg                                  <  2:902708/100‑1 (MQ=255)
                                                                   tcctccTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCGGCTTTAACGGTTCCCGTGCCAGTTGATAGt                                 >  1:757206/1‑100 (MQ=255)
                                                                   tcctccTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGt                                 >  2:2214614/1‑100 (MQ=255)
                                                                       cctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                             <  2:1302847/100‑1 (MQ=255)
                                                                        ctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatca                            >  1:579960/1‑100 (MQ=255)
                                                                         tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                           >  2:1381509/1‑100 (MQ=255)
                                                                         tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGt                                 >  1:2595314/1‑94 (MQ=255)
                                                                         tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                           >  2:1064746/1‑100 (MQ=255)
                                                                          gATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAcc                          <  2:2111261/100‑1 (MQ=255)
                                                                           aTCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCg                         >  1:646051/1‑100 (MQ=255)
                                                                           aTCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAACGCCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCg                         >  1:1098217/1‑100 (MQ=255)
                                                                             cccAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCATTAACGGTTCCCGTGCCAGTTGATAGTCATCACCggg                       >  1:2449523/1‑100 (MQ=255)
                                                                              ccAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgggg                      <  1:2573212/100‑1 (MQ=255)
                                                                               cAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGc                     >  2:2918700/1‑100 (MQ=255)
                                                                               cAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGc                     >  2:1162689/1‑100 (MQ=255)
                                                                                 gCCGCGGTAGTTCTGTCCATACCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                   <  1:2001070/100‑1 (MQ=255)
                                                                                 gCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                   <  2:463100/100‑1 (MQ=255)
                                                                                  ccGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCCGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCggg                  <  1:2372720/100‑1 (MQ=255)
                                                                                   cgcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa                 >  2:1526096/1‑100 (MQ=255)
                                                                                      ggTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGc              >  2:865676/1‑100 (MQ=255)
                                                                                       gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCt             <  2:184607/100‑1 (MQ=255)
                                                                                        tAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCt             >  2:3127804/1‑99 (MQ=255)
                                                                                          gTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc          <  2:939540/100‑1 (MQ=255)
                                                                                          gTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc          >  1:3346986/1‑100 (MQ=255)
                                                                                             cTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAtt       >  1:464048/1‑100 (MQ=255)
                                                                                               gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAGCGGGGCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc     <  1:3014244/100‑1 (MQ=255)
                                                                                               gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc     <  2:1909783/100‑1 (MQ=255)
                                                                                               gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc     >  2:2610563/1‑100 (MQ=255)
                                                                                                 ccGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGa   <  1:964165/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
CACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAT  >  NC_000913/367731‑367928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: