Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,279,370 G→T 56.2% A298S (GCA→TCA)  ghxP → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,3700GT56.2% 31.0 / 198.6 137A298S (GCA→TCA) ghxPguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base T (38/39);  total (69/68)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

Only 100 of 139 total aligned reads displayed.

GTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCG  >  NC_000913/4279271‑4279469
                                                                                                   |                                                                                                   
gTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAg                                                                                                     <  1:700928/100‑1 (MQ=255)
 tgatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                                                                    <  1:126830/100‑1 (MQ=255)
 tgatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                                                                    >  2:126830/1‑100 (MQ=255)
  gatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGca                                                                                                   <  1:2107782/100‑1 (MQ=255)
   atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                                  <  2:3298980/100‑1 (MQ=255)
   atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                                  <  1:1853046/100‑1 (MQ=255)
   atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                                  <  2:2143060/100‑1 (MQ=255)
   atgaCGGCAGTATTTGACGCCAACGGAACTATCAGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcac                                                                                                  <  1:671330/100‑1 (MQ=255)
    tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcact                                                                                                 <  2:1594421/100‑1 (MQ=255)
    tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcact                                                                                                 <  2:2482493/100‑1 (MQ=255)
    tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcact                                                                                                 >  2:2664245/1‑100 (MQ=255)
     gaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactg                                                                                                <  2:2586444/100‑1 (MQ=255)
      aCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactga                                                                                               <  2:2993534/100‑1 (MQ=255)
       cGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCCGGCGACCCTGCTGGGTAAAGATGGGCAGATCATCGACGGTGGGAAATcactg                                                                                                >  1:400518/1‑98 (MQ=39)
       cGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgac                                                                                              >  1:1539539/1‑100 (MQ=255)
       cGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgac                                                                                              <  1:948061/100‑1 (MQ=255)
         gCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgacca                                                                                            >  1:934275/1‑100 (MQ=255)
           aGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccact                                                                                          <  1:765117/100‑1 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactg                                                                                         <  1:2235948/100‑1 (MQ=255)
             tATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactga                                                                                        <  2:2992192/100‑1 (MQ=255)
              aTTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactgac                                                                                       >  2:1384691/1‑100 (MQ=255)
              aTTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactgac                                                                                       >  2:1347964/1‑100 (MQ=255)
               tttGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACt                                                                                      >  2:2658131/1‑100 (MQ=255)
                 tGATGCCACCGGAACTATCCGTGTCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTcc                                                                                    >  1:379851/1‑100 (MQ=255)
                   aTGCCACCGGAACTATCCGTGCCGTCGCCGGGCAGGCGAACATGCTGGATAAAGATGGGCAGAATATCGACGGAGGGAAAGCACTGACAACTGACTCCAt                                                                                  >  1:840675/1‑100 (MQ=37)
                    tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                                 <  1:3371205/100‑1 (MQ=255)
                    tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                                 >  1:1135449/1‑100 (MQ=255)
                    tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATg                                                                                 <  2:303046/100‑1 (MQ=255)
                      ccACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGag                                                                               <  2:2958922/100‑1 (MQ=255)
                       cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagc                                                                              >  2:838505/1‑100 (MQ=255)
                       cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGag                                                                               <  1:3083176/99‑1 (MQ=255)
                           ggAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCg                                                                          >  1:563893/1‑100 (MQ=255)
                                tagccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtc                                                                     <  1:542129/97‑1 (MQ=255)
                                tATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATAAGCAGCGTTTtc                                                                     >  2:1551325/1‑100 (MQ=255)
                                 aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtct                                                                    >  2:553998/1‑100 (MQ=255)
                                   ccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTctct                                                                  <  2:1131164/100‑1 (MQ=255)
                                   ccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTctct                                                                  >  2:3279321/1‑100 (MQ=255)
                                     gTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTgg                                                                >  2:3121720/1‑100 (MQ=255)
                                     gTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTgg                                                                <  2:961503/100‑1 (MQ=255)
                                      tGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                                                               >  1:796244/1‑100 (MQ=255)
                                       gCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGcc                                                              <  2:2358195/100‑1 (MQ=255)
                                          gTCGCCGGCCAGGCGAGCCTGCTGGATAAATATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtgg                                                           <  2:1107881/100‑1 (MQ=255)
                                          gTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtgg                                                           <  2:1844773/100‑1 (MQ=255)
                                            cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtg                                                         >  2:3276498/1‑100 (MQ=255)
                                            cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtg                                                         <  1:339985/100‑1 (MQ=255)
                                             gCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtgg                                                        >  2:1140354/1‑100 (MQ=255)
                                             gCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtgg                                                        <  1:1254242/100‑1 (MQ=255)
                                              ccGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtggg                                                       <  2:563896/100‑1 (MQ=255)
                                                ggCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg                                                     >  2:815547/1‑100 (MQ=255)
                                                ggCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg                                                     <  2:3390621/100‑1 (MQ=255)
                                                ggCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg                                                     >  2:59801/1‑100 (MQ=255)
                                                 ggcAGGCGAACCTGCTGGATAAAGATGGGAAGGTCAGCGAGGGTGGGAAATCACTGACCACTGACTCCATGCGCAGCGTTTTCTCTGGCCTggtggg                                                       <  1:2729325/95‑1 (MQ=255)
                                                 gCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGc                                                    >  2:338297/1‑100 (MQ=255)
                                                 gCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGc                                                    <  1:621000/100‑1 (MQ=255)
                                                  ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtggg                                                       >  1:1390068/1‑96 (MQ=255)
                                                  ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                                   <  2:3044972/100‑1 (MQ=255)
                                                  ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                                   <  2:708446/100‑1 (MQ=255)
                                                   cAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGACATCACTGACCACTGACTCCATGAGCAGCGATTTCTCTGGCCTGGTGGGTGCgg                                                  >  1:1632095/1‑100 (MQ=255)
                                                       cGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTcc                                              >  2:2211445/1‑100 (MQ=255)
                                                          aCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGc                                           <  1:1878852/100‑1 (MQ=255)
                                                           ccTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCa                                          >  1:172550/1‑100 (MQ=255)
                                                           ccTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCa                                          <  2:3211644/100‑1 (MQ=255)
                                                            ctgctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAg                                         >  1:898160/1‑100 (MQ=255)
                                                              gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGTCTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCg                                       <  1:541108/100‑1 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                      <  1:1539537/100‑1 (MQ=255)
                                                                tgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                      <  1:1243677/99‑1 (MQ=255)
                                                                 ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                    <  2:1379462/100‑1 (MQ=255)
                                                                  gATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtat                                   >  2:1188066/1‑100 (MQ=255)
                                                                  gATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtat                                   <  2:2998148/100‑1 (MQ=255)
                                                                  gATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTCTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtat                                   >  2:1811656/1‑100 (MQ=255)
                                                                     aaaGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGTGGCTCCGGCAGCGGtata                                  >  1:3134096/1‑98 (MQ=255)
                                                                       aGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATc                              <  2:532218/100‑1 (MQ=255)
                                                                        gATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCg                             <  2:1401671/100‑1 (MQ=255)
                                                                          tGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAg                           <  1:3328944/100‑1 (MQ=255)
                                                                           nggCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGt                          >  1:2482133/2‑100 (MQ=255)
                                                                           gggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGt                          <  1:107105/100‑1 (MQ=255)
                                                                           gggCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGt                          >  1:2482608/1‑100 (MQ=255)
                                                                             gCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCt                        <  1:1026433/100‑1 (MQ=255)
                                                                               aGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgc                      <  2:143760/100‑1 (MQ=255)
                                                                               aGATCATCGACGGTGGGAAAGCACGGACCGCTGACTCCATGAGCAGCGTTTGCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgc                      <  1:222836/100‑1 (MQ=255)
                                                                                gATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcg                     <  2:2587813/100‑1 (MQ=255)
                                                                                 atcatcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcgg                    >  2:2711190/1‑100 (MQ=255)
                                                                                  tcatcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcgg                    >  1:1362183/1‑99 (MQ=255)
                                                                                   catcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg                  <  2:3055949/100‑1 (MQ=255)
                                                                                   catcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg                  >  1:210083/1‑100 (MQ=255)
                                                                                   catcGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg                  >  2:711772/1‑100 (MQ=255)
                                                                                    atcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGcggcgg                 >  2:2956915/1‑100 (MQ=255)
                                                                                     aggcaagTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCggg                <  2:1711886/94‑1 (MQ=255)
                                                                                      cGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCggg                <  1:1592573/99‑1 (MQ=255)
                                                                                      cGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGt               <  1:2448319/100‑1 (MQ=255)
                                                                                      cGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGAAGCGGTATACATCGAGGCTgcggc                   >  1:2155842/1‑96 (MQ=255)
                                                                                        aCGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTa              <  1:1568741/99‑1 (MQ=255)
                                                                                        aCGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAc             >  1:3078158/1‑100 (MQ=255)
                                                                                         cGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcg            >  2:385027/1‑100 (MQ=255)
                                                                                          ggTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcgg           >  2:1811278/1‑100 (MQ=255)
                                                                                            tGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcg         <  1:3018024/100‑1 (MQ=255)
                                                                                              ggAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggc       >  1:833179/1‑100 (MQ=255)
                                                                                               gAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcg      >  1:3233207/1‑100 (MQ=255)
                                                                                                aaaGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcgg     >  1:455378/1‑100 (MQ=255)
                                                                                                   gCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCggcgggcg  >  2:2058964/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCG  >  NC_000913/4279271‑4279469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: