BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,340,287	G→A	65.0%	L79F (CTT→TTT)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,340,287	0	G	A	65.0%	123.3 / 139.9	143	L79F (CTT→TTT)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand): ref base G (22/28); new base A (44/49); total (66/77)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.03e-01

Only 100 of 143 total aligned reads displayed.

TTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAA  >  NC_000913/4340189‑4340382

ttCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGc                                                                                                <  2:1577876/100‑1 (MQ=255)

  cATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCtt                                                                                              >  1:2044807/1‑100 (MQ=255)

     cGACAAGCTCCAGCAGGCCCCGATCCATTGCGGCGGGGGCTTTTTCCCGATCGCCCAACAGGAAGGCCGGCACGTTTTGTTGGCGCTCATGAAACt                                                                                               <  1:296809/96‑1 (MQ=255)

      gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCg                                                                                          >  2:2852012/1‑100 (MQ=255)

      gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                          >  2:2540790/1‑100 (MQ=255)

        cAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGAt                                                                                        <  1:272166/100‑1 (MQ=255)

         acgCTCCAGCAGGTCGCGATCCATTGCGGCGCGGGCTTTTTCCCGCTCGCCCAACAGGAAGACCGGCACGTTTTGCTGGCGCTCTTGAAACTTCCCGATc                                                                                       >  1:2299603/3‑100 (MQ=255)

            cTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAAt                                                                                    >  2:3013437/1‑100 (MQ=255)

            cTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAAt                                                                                    >  2:3324193/1‑100 (MQ=255)

             tCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAAtt                                                                                   <  1:1961920/100‑1 (MQ=255)

              ccAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTg                                                                                  <  2:2663422/100‑1 (MQ=255)

              ccAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTg                                                                                  <  2:958738/100‑1 (MQ=255)

               cagcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGt                                                                                 >  1:3384804/1‑100 (MQ=255)

                 gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCt                                                                               >  1:1253547/1‑100 (MQ=255)

                  cagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTg                                                                              <  2:1898824/100‑1 (MQ=255)

                  cagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTg                                                                              <  2:1404372/100‑1 (MQ=255)

                  cacgccgcgATCCATTGCGGAGAGGGCTTTTTCCCGAGCCCCCAAAAGGCAGACCGGCACGTTTTGCTGGCGCTAATGCAACTTACCGATCAATTGTCTg                                                                              <  1:1510575/95‑1 (MQ=255)

                   agGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGa                                                                             <  2:505358/100‑1 (MQ=255)

                    ggTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTg                                                                              <  2:1241870/98‑1 (MQ=255)

                        gcgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGttt                                                                        <  1:2929964/100‑1 (MQ=255)

                        gcgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGttt                                                                        <  1:512164/100‑1 (MQ=255)

                          gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGCCGTTTTg                                                                      >  1:1393112/1‑100 (MQ=255)

                          gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTg                                                                      <  1:2581880/100‑1 (MQ=255)

                          gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTg                                                                      >  1:1596905/1‑100 (MQ=255)

                           aTCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGa                                                                     >  1:1835004/1‑100 (MQ=255)

                           aTCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGa                                                                     >  2:1596810/1‑100 (MQ=255)

                             ccATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATg                                                                   >  2:3197643/1‑100 (MQ=255)

                               aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                 <  2:2918517/100‑1 (MQ=255)

                               aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                 >  2:1966864/1‑100 (MQ=255)

                                ttGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGGACGGTTTGTTGGCGCTCATGAAACTTACCGAGCAATTGTCTGAAGTTTTGCTGTTc                                                                >  1:3243303/1‑100 (MQ=255)

                                 tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                               <  1:2513211/100‑1 (MQ=255)

                                 tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                               >  1:827351/1‑100 (MQ=255)

                                    ggcgAGGGCTTTTTCCCGAGCGCTCAACAGGAGGAACGGCGCGTTTTGTTGGCACTAATGACACTCACTGATAAATTGTCTGGTGTTTTGATGTGCGtgc                                                            >  1:2741298/1‑98 (MQ=255)

                                      cgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACTGATCAATTGTCTGACGTTTTGATGTTCGTCCgg                                                          <  2:1733952/100‑1 (MQ=255)

                                       gAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGa                                                         <  2:1883930/100‑1 (MQ=255)

                                        aGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGAt                                                        >  2:1507522/1‑100 (MQ=255)

                                            cTTTTTCCCGATCGCCCAACAGGAAGACCGGCCCTTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTAATGTTCGTCCGGATGTTc                                                    >  1:1637134/1‑100 (MQ=255)

                                            cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGCTCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                    >  1:417570/1‑100 (MQ=255)

                                            cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                    <  1:1354360/100‑1 (MQ=255)

                                            cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                    <  1:1354432/100‑1 (MQ=255)

                                             tttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTcc                                                   >  2:2508442/1‑100 (MQ=255)

                                              ttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCa                                                  <  1:2223016/100‑1 (MQ=255)

                                              ttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCa                                                  <  1:1460592/100‑1 (MQ=255)

                                                ttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                                <  2:2430924/100‑1 (MQ=255)

                                                ttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                                <  1:1006911/100‑1 (MQ=255)

                                                 tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                               <  1:2404485/100‑1 (MQ=255)

                                                 tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                               >  1:2583923/1‑100 (MQ=255)

                                                 tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                               <  2:1106170/100‑1 (MQ=255)

                                                  cccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTg                                              <  1:2327861/100‑1 (MQ=255)

                                                   ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                             >  1:1671890/1‑100 (MQ=255)

                                                    cGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGAt                                            <  2:3384833/100‑1 (MQ=255)

                                                    cGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGAt                                            <  2:2450078/100‑1 (MQ=255)

                                                    cGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGAt                                            <  1:1185204/100‑1 (MQ=255)

                                                       tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGc                                         <  1:1991049/100‑1 (MQ=255)

                                                        cGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCt                                        >  2:410502/1‑100 (MQ=255)

                                                         gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                       >  2:1817091/1‑100 (MQ=255)

                                                         gCCCAACAGGAAGACCGGAACGTTTTGTTGGCGCTCATGAAACTTACCGAGCAATTGTCTGACGTTTTGATGTTCGTCCGGATTTTCCATTTGATAGc                                         >  1:724323/1‑98 (MQ=255)

                                                           ccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGaa                                     <  2:1851064/100‑1 (MQ=255)

                                                             aaCAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGGACa                                   >  2:1093413/1‑100 (MQ=255)

                                                               cAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATc                                 >  1:1684140/1‑100 (MQ=255)

                                                                aGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCa                                <  2:2551545/100‑1 (MQ=255)

                                                                aGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCa                                <  2:969337/100‑1 (MQ=255)

                                                                aGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCa                                >  1:526231/1‑100 (MQ=255)

                                                                 ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCTGGATGTTCCATTTGATAGCTGAACATCa                                >  2:893692/1‑99 (MQ=255)

                                                                 ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                               >  1:2634166/1‑100 (MQ=255)

                                                                   aaGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                             <  2:2154900/100‑1 (MQ=255)

                                                                    aGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCa                            >  2:2776632/1‑100 (MQ=255)

                                                                     gACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAg                           <  2:2355298/100‑1 (MQ=255)

                                                                      aCCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTTTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                             >  1:1755293/1‑97 (MQ=255)

                                                                       ccGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGTACATCAGGCAGTc                         <  2:2278202/100‑1 (MQ=255)

                                                                       ccGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTc                         >  1:1544104/1‑100 (MQ=255)

                                                                       ccGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTc                         >  1:3184410/1‑100 (MQ=255)

                                                                         ggCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCaa                       >  1:2643930/1‑100 (MQ=255)

                                                                         ggCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCaa                       >  1:2904270/1‑100 (MQ=255)

                                                                          gCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAAt                      >  1:1028246/1‑100 (MQ=255)

                                                                           cACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATg                     <  2:3346034/100‑1 (MQ=255)

                                                                            aCGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATgg                    >  1:3034634/1‑100 (MQ=255)

                                                                            aCGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATgg                    <  1:883218/100‑1 (MQ=255)

                                                                             cGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGc                   <  2:328758/100‑1 (MQ=255)

                                                                              gTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGACAGTCAATGGCt                  >  1:979997/1‑100 (MQ=255)

                                                                               ttttGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt                 >  2:1414809/1‑100 (MQ=255)

                                                                               ttttGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt                 <  1:416131/100‑1 (MQ=255)

                                                                               ttttGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt                 >  2:2197130/1‑100 (MQ=255)

                                                                                tttGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTc                <  2:1505481/100‑1 (MQ=255)

                                                                                tttGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTCTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTc                <  1:2377895/100‑1 (MQ=255)

                                                                                      ggCGCTCATGAAACTTACCGATCAATTGTCTGAGGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGa          <  1:3209222/100‑1 (MQ=255)

                                                                                      ggCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGa          <  1:1474294/100‑1 (MQ=255)

                                                                                      ggCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGa          >  1:2496030/1‑100 (MQ=255)

                                                                                       gcgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGa          >  2:3068259/1‑99 (MQ=255)

                                                                                        cgcTTATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGaa         <  2:898882/99‑1 (MQ=255)

                                                                                        cgcTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag        <  1:2178787/100‑1 (MQ=255)

                                                                                        cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATTAGGCAGTCAATGGCTTCGTTTGAag        <  2:2006855/100‑1 (MQ=255)

                                                                                        cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag        <  1:2746902/100‑1 (MQ=255)

                                                                                          cTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATTGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagag      <  1:1817080/100‑1 (MQ=255)

                                                                                           tCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagaga     <  2:3131765/100‑1 (MQ=255)

                                                                                            cATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag    >  1:3339507/1‑100 (MQ=255)

                                                                                            cATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag    <  2:1024874/100‑1 (MQ=255)

                                                                                            cATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag    >  1:2023468/1‑100 (MQ=255)

                                                                                             aTGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga   >  1:1067836/1‑100 (MQ=255)

                                                                                              tGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGaa  >  2:2373499/1‑100 (MQ=255)

TTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAA  >  NC_000913/4340189‑4340382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`