Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,352,460 T→G 67.3% intergenic (+103/‑124) yjdJ → / → ghoS GNAT family putative N‑acetyltransferase/antitoxin of GhoTS toxin‑antitoxin pair; endonuclease for ghoT mRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,352,4600TG67.3% 186.3 / 116.3 132intergenic (+103/‑124)yjdJ/ghoSGNAT family putative N‑acetyltransferase/antitoxin of GhoTS toxin‑antitoxin pair; endonuclease for ghoT mRNA
Reads supporting (aligned to +/- strand):  ref base T (28/15);  new base G (43/45);  total (71/61)
Fisher's exact test for biased strand distribution p-value = 9.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

GTACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCAGAGCG  >  NC_000913/4352365‑4352559
                                                                                               |                                                                                                   
 tACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGgttttt                                                                                                <  1:20827/100‑1 (MQ=255)
  aCAGGGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGGGCATCTGAAGGGTAGGGGGATGCACAACGAATGGGCAGAGAGCTCGgtttt                                                                                                 <  1:918960/98‑1 (MQ=255)
  aCAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGttttttt                                                                                               <  1:2411811/100‑1 (MQ=255)
   cAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTg                                                                                              <  2:2092486/100‑1 (MQ=255)
   cAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTg                                                                                              <  1:2353295/100‑1 (MQ=255)
   cAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTg                                                                                              >  2:2072589/1‑100 (MQ=255)
   cAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTg                                                                                              <  1:2937364/100‑1 (MQ=255)
   cAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTg                                                                                              >  2:2073155/1‑100 (MQ=255)
     gagTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTc                                                                                            >  1:1894396/1‑100 (MQ=255)
      agTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTcc                                                                                           <  2:2375750/100‑1 (MQ=255)
       gTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTccc                                                                                          <  2:1890684/100‑1 (MQ=255)
       gTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTccc                                                                                          <  2:1386483/100‑1 (MQ=255)
       gTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTccc                                                                                          >  2:737456/1‑100 (MQ=255)
       gTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTccc                                                                                          <  2:1005704/100‑1 (MQ=255)
        tCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCa                                                                                         <  1:1738078/100‑1 (MQ=255)
         cACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCaa                                                                                        >  1:1014912/1‑100 (MQ=255)
          aCGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAg                                                                                       >  1:1222363/1‑100 (MQ=255)
          aCGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAg                                                                                       <  2:2393173/100‑1 (MQ=255)
          aCGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAg                                                                                       >  2:2896808/1‑100 (MQ=255)
          aCGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAg                                                                                       <  1:2645168/100‑1 (MQ=255)
           cGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGt                                                                                      <  2:1165417/100‑1 (MQ=255)
           cGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCTCAAGt                                                                                      >  1:1432290/1‑100 (MQ=255)
             atatTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCa                                                                                    >  1:1026095/1‑100 (MQ=255)
             atatTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCa                                                                                    <  1:1497434/100‑1 (MQ=255)
               atTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATc                                                                                  >  2:2671366/1‑100 (MQ=255)
                 ttttCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATccc                                                                                >  1:985721/1‑100 (MQ=255)
                 ttttCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATccc                                                                                >  2:3252579/1‑100 (MQ=255)
                   ttCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCt                                                                              >  2:2514185/1‑100 (MQ=255)
                     cATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCttt                                                                            >  1:61897/1‑100 (MQ=255)
                     cATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCttn                                                                            <  1:32165/100‑2 (MQ=255)
                      aTTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTa                                                                           <  1:1937900/100‑1 (MQ=255)
                         ctctcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTg                                                                        <  1:1472323/100‑1 (MQ=255)
                         ctctcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGACCGTTTTTTTGTCGCAAGTCATGCCTTTTACTg                                                                        >  1:2500305/1‑100 (MQ=255)
                            tcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc                                                                     >  1:2184998/1‑100 (MQ=255)
                             ccGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCa                                                                    >  1:2734809/1‑100 (MQ=255)
                                     tgATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAg                                                            >  2:2112204/1‑100 (MQ=255)
                                      gATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCCGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGa                                                           >  2:452505/1‑100 (MQ=255)
                                       atctaGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGaa                                                          <  1:2359184/96‑1 (MQ=255)
                                           aGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATAtc                                                      <  2:598678/100‑1 (MQ=255)
                                              agagCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGACTATCTcc                                                   <  2:1770279/100‑1 (MQ=255)
                                                agcaaatGAAGGGTAGGGGAATGCACAAAGAATGGGCAGAAAGCGCAGTTTTTTGTCCTAAGTCATCCAATATAAAAAAAAAAAAAAAGAATATCTCCt                                                  <  1:1198716/93‑1 (MQ=255)
                                                 gCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCtat                                                >  2:1601329/1‑100 (MQ=255)
                                                 gCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCtat                                                >  1:208153/1‑100 (MQ=255)
                                                  cATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCtata                                               >  1:378369/1‑100 (MQ=255)
                                                  cATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCtata                                               <  1:1049932/100‑1 (MQ=255)
                                                  cATCTAAAGGGTAGGGGGATGCCCAAAGAATGGGCAGAGAGCGCGGTTCTTTGTCCCAAGTCATCCCCTTTACTAAGCAAAAAAAAGAATATCTCCt                                                  <  1:520621/97‑1 (MQ=255)
                                                    tCTGAAGGGTAGGGGGATGCACAAAGAATGGGCATAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATg                                             <  1:1553252/100‑1 (MQ=255)
                                                      tGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgag                                           <  2:904189/100‑1 (MQ=255)
                                                      tGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgag                                           <  2:1077312/100‑1 (MQ=255)
                                                       gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgaga                                          >  1:600937/1‑100 (MQ=255)
                                                       gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgaga                                          >  2:1714784/1‑100 (MQ=255)
                                                       gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTTTTTTTTTTCTTCACCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgaga                                          >  1:1506955/1‑100 (MQ=255)
                                                       gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgaga                                          <  2:2649195/100‑1 (MQ=255)
                                                       gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgaga                                          >  2:1953967/1‑100 (MQ=255)
                                                       gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgaga                                          >  1:602144/1‑100 (MQ=255)
                                                        aaGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGaa                                         <  2:1986860/100‑1 (MQ=255)
                                                        aaGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGaa                                         <  2:318778/100‑1 (MQ=255)
                                                          gggTAGGGGGATGCACAAAGAATGGGCCGAGAGCGCGGTTTTTTGTCTCATGTCATCCCCTTTATGGAGCAATAAAAGGAATATCTCCTATGTGaaaa                                         >  1:2891954/1‑98 (MQ=255)
                                                           ggTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAatca                                      >  2:1824210/1‑100 (MQ=255)
                                                            gTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAatcat                                     >  2:2177113/1‑100 (MQ=255)
                                                             tAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAatcatc                                    <  2:1704004/100‑1 (MQ=255)
                                                              aGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCAACTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAatcatc                                    <  1:2010964/99‑1 (MQ=255)
                                                               gggggATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCaa                                  >  2:1153872/1‑100 (MQ=255)
                                                               gggggATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCaa                                  >  2:2692726/1‑100 (MQ=255)
                                                                ggggATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTTTTTTTTTTTTTCCCCTATACTGGGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAAt                                 >  1:645838/1‑100 (MQ=255)
                                                                 ggtaTGCACAAAGAATGGGCAGGTAGCGCGGTTTTTTGTCCCACGTCACCACTTTTACTGAGCAAAAAAAAGAATATCGCCTATATGAGAAtcatca                                   <  1:2984755/94‑1 (MQ=255)
                                                                 gggATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATc                                >  1:2210419/1‑100 (MQ=255)
                                                                 gggATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAAt                                 >  1:2407597/1‑99 (MQ=255)
                                                                 gggATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATc                                >  2:1886476/1‑100 (MQ=255)
                                                                      gCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGt                           >  2:1472327/1‑100 (MQ=255)
                                                                       cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGtt                          <  2:3011460/100‑1 (MQ=255)
                                                                       cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGtt                          <  1:1049709/100‑1 (MQ=255)
                                                                       cacaAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGtt                          >  1:3093620/1‑100 (MQ=255)
                                                                       cacaAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGtt                          >  1:428919/1‑100 (MQ=255)
                                                                       cacaAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGtt                          >  2:732856/1‑100 (MQ=255)
                                                                        acaAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCACCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATAGGGGTta                         >  1:100771/1‑100 (MQ=255)
                                                                         caAAGAATGGGCAGAGAGCGCGTTTTTTTTTTTTTTTTTATCCCCTTTACCGAGCAAAACATAGCATCTCTTCTTTTGTTGAATCATCCATCGGAGt                           >  1:3212640/1‑97 (MQ=255)
                                                                         caAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTtaa                        >  2:2570655/1‑100 (MQ=255)
                                                                          aaaGAATGGGCAGATAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTAAAAAAAAAAAAGAATATCTCCTATATGTGAATCATCAATCGGGGtt                          <  1:1054574/97‑1 (MQ=255)
                                                                            aGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTtaataa                     <  2:484507/100‑1 (MQ=255)
                                                                            aGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGACCAAAAAAAAGCATATCTCCTATATGAGAATCATCAATCGGGTTtaataa                     >  1:1077791/1‑100 (MQ=255)
                                                                                tGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGttt                 >  1:2190032/1‑100 (MQ=255)
                                                                                 gggCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGtttt                <  2:3280487/100‑1 (MQ=255)
                                                                                  ggCAGATAGCGCGGTTTTTTGTCCCAATTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg               <  1:2882893/100‑1 (MQ=255)
                                                                                  ggCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGtttt                >  1:713602/1‑99 (MQ=255)
                                                                                  ggCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg               <  1:732853/100‑1 (MQ=255)
                                                                                   gCAGAGAGCGCGTTTTTTTGTCCCAAGTAATCCACTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAAGCCAAAAGCAGGGTTAATAAGTTTTGc              >  1:665460/1‑100 (MQ=255)
                                                                                   gCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGc              <  2:2159942/100‑1 (MQ=255)
                                                                                     agagagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGt            >  2:1963259/1‑100 (MQ=255)
                                                                                      gagagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTc           >  1:1687647/1‑100 (MQ=255)
                                                                                        gagCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTccc         <  1:2587914/100‑1 (MQ=255)
                                                                                         agCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTcccc        <  2:1886222/100‑1 (MQ=255)
                                                                                          gcgcGTTTTTTTGTCCCAAGTCATCCCTTTAACAAAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTcccc        <  1:1338675/99‑1 (MQ=255)
                                                                                          gcgcGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCa       >  2:1361170/1‑100 (MQ=255)
                                                                                          gcgcGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCTGAGTAAATAAGTTTTGCTTCCCCa       >  1:122742/1‑100 (MQ=255)
                                                                                           cgcgTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCag      >  2:983818/1‑100 (MQ=255)
                                                                                            gcgGTTTTTTGTCCCATGTCATCCCATTTACTGAGCAAAAAAAAAAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGtgc          >  1:1097417/1‑93 (MQ=255)
                                                                                             cgTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCagag    >  2:240814/1‑100 (MQ=255)
                                                                                              gTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCAGAGc   >  2:2346607/1‑100 (MQ=255)
                                                                                               tttttttGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCAGAGCg  <  2:2958732/100‑1 (MQ=255)
                                                                                               |                                                                                                   
GTACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCAGAGCG  >  NC_000913/4352365‑4352559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: