Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,360,814 T→G 51.1% Y374S (TAC→TCC)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,360,8140TG51.1% 21.6 / 226.0 149Y374S (TAC→TCC) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (35/38);  new base G (39/37);  total (74/75)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 150 total aligned reads displayed.

TGTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCCTGAT  >  NC_000913/4360716‑4360913
                                                                                                  |                                                                                                   
tGTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTa                                                                                                    <  1:2552498/100‑1 (MQ=255)
 gTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAg                                                                                                   <  2:453849/100‑1 (MQ=255)
  ttATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAgg                                                                                                  <  2:1303483/100‑1 (MQ=255)
  ttATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAgg                                                                                                  <  2:2159789/100‑1 (MQ=255)
     tCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaa                                                                                               >  2:634739/1‑100 (MQ=255)
     tCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGAGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTaa                                                                                               >  1:1383235/1‑100 (MQ=255)
       tATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAAt                                                                                             <  1:2704059/100‑1 (MQ=255)
        aTTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAtt                                                                                            >  1:2938059/1‑100 (MQ=255)
         tttCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTc                                                                                           <  2:521930/100‑1 (MQ=255)
           tCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTg                                                                                         >  2:2434928/1‑100 (MQ=255)
           tCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTg                                                                                         >  2:2632446/1‑100 (MQ=255)
            cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTgg                                                                                        <  1:2473555/100‑1 (MQ=255)
             atctttCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                       <  1:1486594/96‑1 (MQ=255)
              gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                      <  2:586620/100‑1 (MQ=255)
                 ttCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggagat                                                                                   >  2:1486600/1‑96 (MQ=255)
                  tCAGTGCTGCTAATTGTTTTTCCTCTAAAGGATGTTGAGATTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGGGCAATCTGGGgttga                                                                                  >  2:2901897/1‑100 (MQ=255)
                  tCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgatga                                                                                  <  2:2562237/100‑1 (MQ=255)
                   cAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgatgat                                                                                 <  2:1304170/100‑1 (MQ=255)
                    aGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGAtt                                                                                <  1:2258352/100‑1 (MQ=255)
                    aGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTATGGGGATGAtt                                                                                >  2:3171100/1‑100 (MQ=255)
                     gTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTg                                                                               >  2:773254/1‑100 (MQ=255)
                     gTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTg                                                                               <  1:2085466/100‑1 (MQ=255)
                     gTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTg                                                                               <  1:1328175/100‑1 (MQ=255)
                        ctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAAc                                                                            >  2:3057259/1‑100 (MQ=255)
                        ctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAAc                                                                            >  2:1828618/1‑100 (MQ=255)
                         tgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACa                                                                           >  1:1892079/1‑100 (MQ=255)
                         tgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACa                                                                           >  1:599086/1‑100 (MQ=255)
                         tgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACa                                                                           <  2:604055/100‑1 (MQ=255)
                          gctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACaa                                                                          >  2:2749949/1‑100 (MQ=255)
                          gctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACaa                                                                          >  1:43120/1‑100 (MQ=255)
                              aTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAAtctc                                                                      <  1:2834827/100‑1 (MQ=255)
                                tGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCAc                                                                    <  1:21898/100‑1 (MQ=255)
                                 gattttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTATCTGCTATCGCGGAGGTAAATTCTGTGGATGATTGAACAATCTCAcc                                                                   >  1:527457/3‑100 (MQ=255)
                                 gTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAcc                                                                   >  1:2202999/1‑100 (MQ=255)
                                 gTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAcc                                                                   >  2:386177/1‑100 (MQ=255)
                                  tttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCt                                                                  <  2:2260770/100‑1 (MQ=255)
                                  tttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCt                                                                  <  1:2763630/100‑1 (MQ=255)
                                   ttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGTTTGACCCATCTCAcgt                                                                  >  1:658214/1‑97 (MQ=255)
                                   ttttCATCTAAAGGATGTTGAGAATGGAGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAGCta                                                                 >  1:1800943/1‑100 (MQ=255)
                                      tCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaata                                                              <  2:3107870/100‑1 (MQ=255)
                                      tCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAAGTAAATTCTGGGGATGATTGAACAATCTCACCtaata                                                              <  1:1814093/100‑1 (MQ=255)
                                        aTCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTaataat                                                            >  2:137310/1‑100 (MQ=255)
                                          cTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTc                                                          <  2:530296/100‑1 (MQ=255)
                                            aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                        >  2:1889684/1‑100 (MQ=255)
                                            aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                        <  2:3203802/100‑1 (MQ=255)
                                            aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                        <  2:739528/100‑1 (MQ=255)
                                            aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGGACAATCTCACCTAATAATTCAc                                                        <  1:37968/100‑1 (MQ=255)
                                              aGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTg                                                      >  2:2163579/1‑100 (MQ=255)
                                               ggATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTgg                                                     <  1:1999972/100‑1 (MQ=255)
                                                 aTGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCa                                                   >  2:17566/1‑100 (MQ=255)
                                                   gTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACg                                                 >  2:1742360/1‑100 (MQ=255)
                                                     tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGt                                               >  1:2420624/1‑100 (MQ=255)
                                                     tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGt                                               <  1:2465528/100‑1 (MQ=255)
                                                       agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                             <  1:3077076/100‑1 (MQ=255)
                                                       agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                             <  2:2039727/100‑1 (MQ=255)
                                                        gaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCa                                            >  2:1496523/1‑100 (MQ=255)
                                                        gaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCa                                            >  1:3050241/1‑100 (MQ=255)
                                                          aTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCTCTGGCACGGTTCAAt                                          >  2:3340813/1‑100 (MQ=255)
                                                          aTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAAt                                          <  1:3171073/100‑1 (MQ=255)
                                                          aTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAAt                                          <  1:302130/100‑1 (MQ=255)
                                                          aTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAAt                                          <  1:148083/100‑1 (MQ=255)
                                                          aTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCATAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                             <  1:2371772/97‑1 (MQ=255)
                                                            ggtgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAAt                                          <  1:2834819/95‑1 (MQ=255)
                                                             gcgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAt                                       >  1:1571165/1‑100 (MQ=255)
                                                              cgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAtt                                      <  2:2038414/100‑1 (MQ=255)
                                                              cgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAtt                                      >  1:1838562/1‑100 (MQ=255)
                                                               gcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                                     >  2:10588/1‑100 (MQ=255)
                                                                cACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTa                                    >  2:910005/1‑100 (MQ=255)
                                                                  cGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatc                                  >  1:1040244/1‑100 (MQ=255)
                                                                  cGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatc                                  <  1:133322/100‑1 (MQ=255)
                                                                  cGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatc                                  <  1:1832096/100‑1 (MQ=255)
                                                                     tatCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatcatc                               <  2:691336/100‑1 (MQ=255)
                                                                     tatCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatcatc                               <  1:1715229/100‑1 (MQ=255)
                                                                       tCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGc                             <  2:1367457/100‑1 (MQ=255)
                                                                       tCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGc                             <  2:3208933/100‑1 (MQ=255)
                                                                        cAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGc                             <  2:675379/99‑1 (MQ=255)
                                                                        cAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGcc                            >  2:776178/1‑100 (MQ=255)
                                                                        cAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGcc                            >  1:379951/1‑100 (MQ=255)
                                                                            tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                        >  2:1145934/1‑100 (MQ=255)
                                                                            tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                        <  1:2778312/100‑1 (MQ=255)
                                                                              aTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAg                      >  2:387144/1‑100 (MQ=255)
                                                                               tGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAgn                     <  1:2619914/100‑2 (MQ=255)
                                                                               tGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGt                     >  1:902494/1‑100 (MQ=255)
                                                                               ngCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAg                      >  1:3037917/2‑99 (MQ=255)
                                                                               ngCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGt                     >  1:73/2‑100 (MQ=255)
                                                                                gCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa                    >  1:1088789/1‑100 (MQ=255)
                                                                                gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGt                     >  2:3212101/1‑99 (MQ=255)
                                                                                gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa                    <  2:927718/100‑1 (MQ=255)
                                                                                  tttttCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaaa                  >  1:2918530/1‑100 (MQ=255)
                                                                                  tttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaaa                  >  2:2706667/1‑100 (MQ=255)
                                                                                    tttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaata                >  1:2312902/1‑100 (MQ=255)
                                                                                     ttCTGCTCTCGCGTAGGTAAAGTCTGGAGATGATTCAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa                    <  1:1190850/95‑1 (MQ=255)
                                                                                       cTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATc             <  1:1115295/100‑1 (MQ=255)
                                                                                       cTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTACTAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATc             >  2:2726736/1‑100 (MQ=255)
                                                                                       cTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATc             >  1:2551208/1‑100 (MQ=255)
                                                                                        tGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa            <  2:402955/100‑1 (MQ=255)
                                                                                          ctctCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtg          >  1:3181569/1‑100 (MQ=255)
                                                                                          ctctCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtg          <  1:1893017/100‑1 (MQ=255)
                                                                                              cgcgGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGcc      >  2:2277845/1‑100 (MQ=255)
                                                                                                  tAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCCTGAt  >  1:1425175/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TGTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCCTGAT  >  NC_000913/4360716‑4360913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: