Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 383,477 G→A 68.2% G153G (GGC→GGT yaiP ← putative family 2 glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913383,4770GA68.2% 154.6 / 113.7 132G153G (GGC→GGTyaiPputative family 2 glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base A (45/45);  total (66/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 132 total aligned reads displayed.

TAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAAC  >  NC_000913/383380‑383572
                                                                                                 |                                                                                               
taataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGcc                                                                                               <  1:2373004/100‑1 (MQ=255)
taataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTGCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCAcc                                                                                               <  1:2963577/100‑1 (MQ=255)
 aataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCt                                                                                              >  1:3604/1‑100 (MQ=255)
 aataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCt                                                                                              >  1:2987624/1‑100 (MQ=255)
  ataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCtt                                                                                             <  2:3213880/100‑1 (MQ=255)
  ataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCtt                                                                                             >  1:659304/1‑100 (MQ=255)
  ataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCtt                                                                                             >  1:994707/1‑100 (MQ=255)
   taaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCttt                                                                                            >  1:1480498/1‑100 (MQ=255)
    aaaCGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTc                                                                                           <  2:2510264/100‑1 (MQ=255)
    aaaCGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTc                                                                                           <  2:3232682/100‑1 (MQ=255)
         gTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGc                                                                                      <  2:1942433/100‑1 (MQ=255)
         gTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGc                                                                                      <  2:1112578/100‑1 (MQ=255)
           gcgcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAg                                                                                    <  1:2362110/100‑1 (MQ=255)
            cgcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGt                                                                                   <  2:2929770/100‑1 (MQ=255)
             gcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGt                                                                                   >  1:2349952/1‑99 (MQ=255)
             gcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTa                                                                                  >  2:406301/1‑100 (MQ=255)
             gcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGACAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTa                                                                                  >  1:1879110/1‑100 (MQ=255)
               cACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTaga                                                                                >  1:1204492/1‑100 (MQ=255)
                 cccAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAgg                                                                              >  2:2738430/1‑100 (MQ=255)
                 cccAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAgg                                                                              <  2:1637760/100‑1 (MQ=255)
                   cAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCCGCACCTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGa                                                                            <  1:992315/100‑1 (MQ=255)
                   cAGGAGCTGCTGTAGCGTGCGCTTCATAACAAGCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAggg                                                                             <  1:3319150/99‑1 (MQ=255)
                      gAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATg                                                                         >  2:1345002/1‑100 (MQ=255)
                      gAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATg                                                                         <  1:1344996/100‑1 (MQ=255)
                      ccgatcttgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATg                                                                         <  2:1110789/93‑1 (MQ=255)
                         ctgctgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATgccg                                                                      >  1:1167837/1‑100 (MQ=255)
                         ctgctgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATgccg                                                                      <  2:2131141/100‑1 (MQ=255)
                          tgctgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATgccgc                                                                     <  1:454869/100‑1 (MQ=255)
                          tgctgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATgccgc                                                                     >  2:1534771/1‑100 (MQ=255)
                           gctgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATgccgcc                                                                    >  2:1402294/1‑100 (MQ=255)
                           gctgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATgccgcc                                                                    >  2:932463/1‑100 (MQ=255)
                             tgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCt                                                                   >  1:2043819/1‑99 (MQ=255)
                             tgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATgagatcgg                                                                  >  1:1110786/1‑93 (MQ=255)
                                  cGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGCTGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCa                                                             >  2:2963991/1‑100 (MQ=255)
                                   gTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATTCCGCCTACGGctt                                                            <  2:2301513/100‑3 (MQ=255)
                                   gTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCAt                                                            >  1:1727040/1‑100 (MQ=255)
                                    tGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATc                                                           <  2:1624930/100‑1 (MQ=255)
                                     gcgcTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCg                                                          <  2:880053/100‑1 (MQ=255)
                                     gcgcTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCg                                                          >  1:2242379/1‑100 (MQ=255)
                                     gcgcTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATATGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCg                                                          >  1:1921208/1‑100 (MQ=255)
                                      cgcTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCgg                                                         >  2:1478914/1‑100 (MQ=255)
                                      cgcTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCgg                                                         >  1:159794/1‑100 (MQ=255)
                                        cTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCa                                                       >  2:2192548/1‑100 (MQ=255)
                                           cATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACcg                                                    >  1:1412506/1‑100 (MQ=255)
                                            aTAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACcgc                                                   >  2:796818/1‑100 (MQ=255)
                                              aaCAATCATCGGCAACTTTACGGTCGCGCGGATGTTCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCgcgc                                                 <  2:2034303/100‑1 (MQ=255)
                                               aCAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCt                                                >  2:26606/1‑100 (MQ=255)
                                                 aaTCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCa                                              <  1:167566/100‑1 (MQ=255)
                                                 aaTCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCa                                              <  2:39492/100‑1 (MQ=255)
                                                  atcatcGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCaa                                             >  1:3193711/1‑100 (MQ=255)
                                                    catcGACAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAAtt                                           <  1:1368835/100‑1 (MQ=255)
                                                     atcGGCAACTTTACGGTCGCGCGGATGTGCGATAACACACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAAttt                                          <  1:2282785/100‑1 (MQ=255)
                                                      tcGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTc                                         <  2:659307/100‑1 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       >  2:1174167/1‑100 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       >  2:1770674/1‑100 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       <  1:1401467/100‑1 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       <  1:1178606/100‑1 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       <  2:3041486/100‑1 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       >  2:2341076/1‑100 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       <  2:2810736/100‑1 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       >  1:1054913/1‑100 (MQ=255)
                                                        ggCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTg                                       >  1:1030967/1‑100 (MQ=255)
                                                         gCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGc                                      <  1:1031264/100‑1 (MQ=255)
                                                          cAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGcc                                     <  1:2718693/100‑1 (MQ=255)
                                                          cAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGcc                                     <  2:159794/100‑1 (MQ=255)
                                                           aaCTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCa                                    <  1:879071/100‑1 (MQ=255)
                                                            aCTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAg                                   >  2:2022540/1‑100 (MQ=255)
                                                              tttACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCa                                 <  1:212690/100‑1 (MQ=255)
                                                                  cGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCAtata                             >  2:267936/1‑100 (MQ=255)
                                                                    gTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGc                           <  1:1032759/100‑1 (MQ=255)
                                                                    gTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGc                           <  1:2196999/100‑1 (MQ=255)
                                                                    gTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGc                           <  2:2242395/100‑1 (MQ=255)
                                                                      ctcgcgGATGTGCAGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGccc                         >  2:2293971/3‑100 (MQ=255)
                                                                      cgcgcgGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGccc                         <  2:2963426/100‑1 (MQ=255)
                                                                      cgcacgGATGTGCGGTAACAGACCCACACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGACACCAACCTAAATTTCTGCCAGCa                                 >  1:2131416/1‑92 (MQ=255)
                                                                        cgcgGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCAt                       >  2:1541210/1‑100 (MQ=255)
                                                                        cgcgGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCAt                       >  2:354378/1‑100 (MQ=255)
                                                                          cgGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTc                     >  2:1805426/1‑100 (MQ=255)
                                                                          cgGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTc                     >  2:693818/1‑100 (MQ=255)
                                                                          cgGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTc                     >  2:1979606/1‑100 (MQ=255)
                                                                            gATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCg                   >  2:2563950/1‑100 (MQ=255)
                                                                               gtgCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCt                >  1:473389/1‑100 (MQ=255)
                                                                               gtgCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCt                <  2:2793071/100‑1 (MQ=255)
                                                                                tgCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCtt               >  1:2388454/1‑100 (MQ=255)
                                                                                tgCGGTAACAGACCCGCAACTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCtt               <  1:1101261/100‑1 (MQ=255)
                                                                                 gCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTg              <  1:1330216/100‑1 (MQ=255)
                                                                                 gCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGAGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTg              >  1:2563940/1‑100 (MQ=255)
                                                                                   ggTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGAt            >  1:981819/1‑100 (MQ=255)
                                                                                   ggTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGAt            >  1:3358381/1‑100 (MQ=255)
                                                                                      aaCAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCgg         >  1:2488278/1‑100 (MQ=255)
                                                                                       aCAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCggg        <  2:3099681/100‑1 (MQ=255)
                                                                                         aGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATGTAGCCCATTCCGTCTTGATCGGGCg      <  1:1663989/100‑1 (MQ=255)
                                                                                         aGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCg      <  2:120562/100‑1 (MQ=255)
                                                                                         aGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCg      <  1:2900270/100‑1 (MQ=255)
                                                                                         aGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCg      <  2:1358537/100‑1 (MQ=255)
                                                                                          gACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCgg     <  1:1082297/100‑1 (MQ=255)
                                                                                            cccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGaa   >  1:635976/1‑100 (MQ=255)
                                                                                             ccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCGATGTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAAc  >  2:631137/1‑100 (MQ=255)
                                                                                             ccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAAc  >  2:3131469/1‑100 (MQ=255)
                                                                                             ccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAAc  >  2:3288184/1‑100 (MQ=255)
                                                                                                 |                                                                                               
TAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAAC  >  NC_000913/383380‑383572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: