Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 400,214 T→C 67.5% D237G (GAC→GGC)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,2140TC67.5% 139.3 / 85.0 109D237G (GAC→GGC) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (22/13);  new base C (39/34);  total (61/48)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 109 total aligned reads displayed.

ACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTG  >  NC_000913/400118‑400310
                                                                                                |                                                                                                
aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGt                                                                                               >  1:951157/1‑100 (MQ=255)
aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGt                                                                                               >  1:548110/1‑100 (MQ=255)
  naCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTg                                                                                             >  1:1238461/2‑100 (MQ=255)
   aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTCGGCATAGAAATCGCTGGTTAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTg                                                                                             >  2:3219623/1‑99 (MQ=255)
   aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGc                                                                                            <  1:2995308/100‑1 (MQ=255)
   aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGc                                                                                            <  2:436217/100‑1 (MQ=255)
    cTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGcc                                                                                           >  2:1387282/1‑100 (MQ=255)
     tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGccc                                                                                          >  1:628825/1‑100 (MQ=255)
     tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGccc                                                                                          >  1:1357346/1‑100 (MQ=255)
         gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGaa                                                                                      >  2:2145000/1‑100 (MQ=255)
         gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGaa                                                                                      >  2:2318150/1‑100 (MQ=255)
          cgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAAc                                                                                     <  2:1444949/100‑1 (MQ=255)
           gcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACt                                                                                    <  2:1774881/100‑1 (MQ=255)
              atattCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACGGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                                 <  1:1502072/97‑1 (MQ=255)
              aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgcg                                                                                 <  1:923947/100‑1 (MQ=255)
              aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTgcg                                                                                 >  1:2842482/1‑100 (MQ=255)
              aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                                 <  2:166659/100‑1 (MQ=255)
              aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                                 <  1:380428/100‑1 (MQ=255)
              aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                                 <  2:679120/100‑1 (MQ=255)
              aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                                 <  1:1062527/100‑1 (MQ=255)
                cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCa                                                                               >  2:1516188/1‑100 (MQ=255)
                 ttCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCAt                                                                              <  2:461027/100‑1 (MQ=255)
                   cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                            <  1:2318133/100‑1 (MQ=255)
                   cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                            >  1:2631277/1‑100 (MQ=255)
                     tCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGa                                                                          >  2:842702/1‑100 (MQ=255)
                      cAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAt                                                                         >  2:2564760/1‑100 (MQ=255)
                        aTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGAtct                                                                       >  2:1910147/1‑100 (MQ=255)
                         tGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtctc                                                                      >  1:3285659/1‑100 (MQ=255)
                          gTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCa                                                                     <  1:1758/100‑1 (MQ=255)
                           tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTGCGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                    >  1:1944536/1‑100 (MQ=255)
                           tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                    >  2:302231/1‑100 (MQ=255)
                           tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtctc                                                                      >  1:1437701/1‑98 (MQ=255)
                           tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                    <  2:2631297/100‑1 (MQ=255)
                             cTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGa                                                                  >  2:2226363/1‑100 (MQ=255)
                              ttGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTACACAGGTGCTGACTTGAGGATTGCCGCTGCCCAGAACTGCGCATTCGATCCCACGAc                                                                 >  1:271346/1‑100 (MQ=255)
                               tGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGAcc                                                                >  2:2615043/1‑100 (MQ=255)
                                 gtgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCtt                                                              <  2:805691/100‑1 (MQ=255)
                                  tgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                             >  1:2250051/1‑100 (MQ=255)
                                  tgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                             <  1:2598316/100‑1 (MQ=255)
                                  tgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                             <  2:386680/100‑1 (MQ=255)
                                   gtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTg                                                            >  2:3101511/1‑100 (MQ=255)
                                      gTAGGCATAGAAGTCGCTGGTGGGTACGATCTGTGGACAGGTGCTGGCTTGCGGATTGTCGTGGCTGAAAACTGCGCATTCGATATGAGGACCGTTg                                                            >  1:3366841/1‑97 (MQ=255)
                                      gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                         >  1:3369331/1‑100 (MQ=255)
                                      gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                         >  1:1644272/1‑100 (MQ=255)
                                      gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                         >  1:138378/1‑100 (MQ=255)
                                           cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTTCGGATTGCCTTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                                      <  1:1386428/98‑1 (MQ=255)
                                           cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                    >  2:79378/1‑100 (MQ=255)
                                           cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                    >  2:838344/1‑100 (MQ=255)
                                            aTAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                   <  1:289109/100‑1 (MQ=255)
                                            aTAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                   >  1:910102/1‑100 (MQ=255)
                                              aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                                 >  2:2522645/1‑100 (MQ=255)
                                               gAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGTCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCa                                                <  1:2141098/100‑1 (MQ=255)
                                               gAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCa                                                >  2:229291/1‑100 (MQ=255)
                                                  aTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGTTCTCACGACCTTTGATCACTTGCTCAACg                                             >  1:2650485/1‑100 (MQ=255)
                                                  aTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACg                                             >  1:2614221/1‑100 (MQ=255)
                                                   tCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGa                                            <  1:2732091/100‑1 (MQ=255)
                                                   tCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGAGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGa                                            <  1:1838607/100‑1 (MQ=255)
                                                     gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                          >  2:1342531/1‑100 (MQ=255)
                                                     gCTGGTGAGTACGATCTCTCCACAGGTGCTAGCGTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTACTCAACGATc                                          >  1:2223188/1‑100 (MQ=255)
                                                      cTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                         >  2:3278737/1‑100 (MQ=255)
                                                       tGGTGAGTCCGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCAc                                        <  1:2386694/100‑1 (MQ=255)
                                                         gTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACtt                                      <  1:1708823/100‑1 (MQ=255)
                                                           gAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                    <  2:2044512/100‑1 (MQ=255)
                                                           gAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                    >  1:2165512/1‑100 (MQ=255)
                                                             gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                                  >  1:270396/1‑100 (MQ=255)
                                                              tACGATCTCTCCACAGGTGCTGGCTTGCGGAGTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGa                                 <  2:180096/100‑1 (MQ=255)
                                                                cGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATggct                               >  1:2494065/1‑97 (MQ=255)
                                                                cGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                               <  1:1028503/100‑1 (MQ=255)
                                                                cGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                               <  1:2226348/100‑1 (MQ=255)
                                                                 gATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCCCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                              <  1:243882/100‑1 (MQ=255)
                                                                  aTCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgn                             <  2:1238465/100‑2 (MQ=255)
                                                                   tctctcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaa                            <  2:1097432/100‑1 (MQ=255)
                                                                   tctctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaa                            >  1:332885/1‑100 (MQ=255)
                                                                    ctctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                           <  2:910105/100‑1 (MQ=255)
                                                                    ctctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                           <  2:866047/100‑1 (MQ=255)
                                                                    ctctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                           <  1:1351032/100‑1 (MQ=255)
                                                                    ctctcCACAGGTGCTGGCTTGCGGATTGCCGATGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCACTTGCTCAACGATCACTTCATGAtcgaac                           >  1:2712107/1‑100 (MQ=255)
                                                                     tctcCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                          >  1:2217666/1‑100 (MQ=255)
                                                                     tctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                          >  2:143791/1‑100 (MQ=255)
                                                                     tctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                          <  1:891302/100‑1 (MQ=255)
                                                                      ctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactc                         >  2:2723832/1‑100 (MQ=255)
                                                                          acaGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaac                     >  1:2581064/1‑100 (MQ=255)
                                                                           caGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACg                    <  2:2922596/100‑1 (MQ=255)
                                                                           caGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACg                    <  2:1671133/100‑1 (MQ=255)
                                                                            aGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGc                   <  2:1357352/100‑1 (MQ=255)
                                                                            aGGTGCTGGCGTGCGGCTTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGCTCTCTTTATGATCGAACTCGAACg                    <  1:640338/99‑1 (MQ=255)
                                                                             ggTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcc                  <  2:1297601/100‑1 (MQ=255)
                                                                              gTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCa                 <  2:3312446/100‑1 (MQ=255)
                                                                               tGCTGGCTTGCGGCTTGGCGTTGCCCAGAACGCCGCCTTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAccgaactcgaac                     <  1:956099/95‑1 (MQ=255)
                                                                                 cTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt              >  1:2491933/1‑100 (MQ=255)
                                                                                 cTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt              >  2:2978769/1‑100 (MQ=255)
                                                                                 cTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt              >  2:2198215/1‑100 (MQ=255)
                                                                                    gCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGa           >  2:410568/1‑100 (MQ=255)
                                                                                      ttGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACg         >  1:350598/1‑100 (MQ=255)
                                                                                        gCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGc       >  2:1272804/1‑100 (MQ=255)
                                                                                        gCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGc       <  2:2130424/100‑1 (MQ=255)
                                                                                         cGGAGTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGc       <  1:64726/99‑1 (MQ=255)
                                                                                           gATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCCCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCaan    <  1:2514079/100‑2 (MQ=255)
                                                                                             ttGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAAtt   >  1:3269315/1‑99 (MQ=255)
                                                                                             ttGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTg  <  1:842699/100‑1 (MQ=255)
                                                                                                |                                                                                                
ACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTG  >  NC_000913/400118‑400310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑