Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 409,322 G→T 23.8% A233E (GCG→GAG)  rdgC ← nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913409,3220GT23.8% 330.8 / 95.0 154A233E (GCG→GAG) rdgCnucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function
Reads supporting (aligned to +/- strand):  ref base G (56/61);  new base T (20/17);  total (76/78)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

ATATCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCAGCA  >  NC_000913/409223‑409421
                                                                                                   |                                                                                                   
atatCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAgcg                                                                                                     <  1:2626095/100‑1 (MQ=255)
  atCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGcc                                                                                                   >  1:932902/1‑100 (MQ=255)
   tCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCa                                                                                                  >  2:1082374/1‑100 (MQ=255)
    cTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAg                                                                                                 >  2:2735953/1‑100 (MQ=255)
    cTTCGTTTTGATCGCGCAGCGCGTCGCCCAACTTCAGACGCTTGAACGAACCACCGGCCCACATCTCAAACTTACTGCGCTGCTCCCAGTCgcgcgc                                                                                                    >  1:2763687/1‑97 (MQ=255)
        gTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTa                                                                                             >  2:1120083/1‑100 (MQ=255)
        gTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGACCGCCAGTTTa                                                                                             >  1:2232024/1‑100 (MQ=255)
         ttttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAg                                                                                            <  2:1682140/100‑1 (MQ=255)
           ttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTc                                                                                          <  2:2610804/100‑1 (MQ=255)
           ttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTc                                                                                          <  1:187196/100‑1 (MQ=255)
           ttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTc                                                                                          <  2:3282268/100‑1 (MQ=255)
           ttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACAATCGTCGCACATTACAACCTGAATGCGCTGCTGCCAGCCGAGCTCCAGTTTAGt                                                                                           >  1:2371930/1‑99 (MQ=255)
           ttGATCGCGCAGCTCGTCGCAGAACTGCAGACGCTTGTGCGAACCAGCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGGCGAGCTCCAGTTTAGTc                                                                                          >  1:2019901/1‑100 (MQ=255)
             gATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCCCAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTCTAGTcac                                                                                        >  2:2470106/1‑100 (MQ=255)
             gATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTcac                                                                                        >  1:19997/1‑100 (MQ=255)
             gATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTca                                                                                         >  2:364307/1‑99 (MQ=255)
                cgcgCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTcaccac                                                                                     <  2:206485/100‑1 (MQ=255)
                cgcgCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTcaccac                                                                                     >  1:1740055/1‑100 (MQ=255)
                    cAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACtttt                                                                                 >  1:3380910/1‑100 (MQ=255)
                     aGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACtttt                                                                                 >  1:116647/1‑99 (MQ=255)
                      gCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTcc                                                                               <  1:2579291/100‑1 (MQ=255)
                       cTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCg                                                                              >  2:2400890/1‑100 (MQ=255)
                       cTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCg                                                                              <  1:1020151/100‑1 (MQ=255)
                        tcgtcgCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCgg                                                                             >  1:714910/1‑100 (MQ=255)
                           tcgCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCtt                                                                          <  1:2147447/100‑1 (MQ=255)
                            cgCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTc                                                                         <  2:2181492/100‑1 (MQ=255)
                            cgCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTc                                                                         <  2:2459130/100‑1 (MQ=255)
                             gCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCa                                                                        >  2:425301/1‑100 (MQ=255)
                                gAACTTCAGACGCTTGAGCTAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtg                                                                     <  1:810248/100‑1 (MQ=255)
                                gAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtg                                                                     >  1:16971/1‑100 (MQ=255)
                                gAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtg                                                                     <  2:3094016/100‑1 (MQ=255)
                                 aaCTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCAAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgt                                                                    <  1:3177652/100‑1 (MQ=255)
                                  aCTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgtg                                                                   >  1:589164/1‑100 (MQ=255)
                                  aCTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgtg                                                                   <  2:1238312/100‑1 (MQ=255)
                                  aCTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgtg                                                                   >  2:1423640/1‑100 (MQ=255)
                                   cTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGa                                                                  >  2:2899880/1‑100 (MQ=255)
                                   cTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGa                                                                  >  2:1533907/1‑100 (MQ=255)
                                     tCAGACGCTTGGGCGAACCAGCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGGGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgt                                                                    <  1:689228/96‑1 (MQ=255)
                                      cAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTg                                                               >  2:2616188/1‑100 (MQ=255)
                                        gACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGt                                                             >  2:2844737/1‑100 (MQ=255)
                                          cGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGa                                                           <  1:1412319/100‑1 (MQ=255)
                                          cGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGa                                                           >  2:1921965/1‑100 (MQ=255)
                                            cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc                                                         <  2:93068/100‑1 (MQ=255)
                                            cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc                                                         >  1:2105280/1‑100 (MQ=255)
                                            cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc                                                         <  1:526497/100‑1 (MQ=255)
                                            cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc                                                         >  1:485195/1‑100 (MQ=255)
                                             ttGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtct                                                        <  1:1201566/100‑1 (MQ=255)
                                                aGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCtt                                                     >  2:2932852/1‑100 (MQ=255)
                                                aGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCtt                                                     <  1:2738343/100‑1 (MQ=255)
                                                aGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCtt                                                     >  2:951447/1‑100 (MQ=255)
                                                aGCGAACCATCGGCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCGGTTTTCCGGCTTCAATGTGATTGGTGAtct                                                        >  1:416739/1‑97 (MQ=255)
                                                aGCGAACCAGCGTCGCACATCACATACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCtt                                                     <  2:2893075/100‑1 (MQ=255)
                                                   gAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGc                                                  >  2:2806855/1‑100 (MQ=255)
                                                   gAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGc                                                  <  2:756922/100‑1 (MQ=255)
                                                   gAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGc                                                  <  1:1902436/100‑1 (MQ=255)
                                                   gAACCATCGTCACACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGc                                                  <  2:2551996/100‑1 (MQ=255)
                                                      ccATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTgg                                               <  1:2400873/100‑1 (MQ=255)
                                                      ccATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTgg                                               <  2:3165495/100‑1 (MQ=255)
                                                      ccATCGTCGCACATCACAAACTGAATGCGCTACTGCCAGTCGAGCTCCAGATTACACACCACTTTTCCCGCTACAATGTGATTGGTGATCTCTTCGca                                                 >  1:1345554/1‑97 (MQ=255)
                                                       cttcgtcgCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGt                                              <  1:2058693/98‑1 (MQ=255)
                                                        aTCGTCCCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTc                                             <  1:2851534/100‑1 (MQ=255)
                                                             cgCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATc                                        <  2:963836/100‑1 (MQ=255)
                                                                 caTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGt                                    >  1:962555/1‑100 (MQ=255)
                                                                 caTCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGt                                    >  1:394911/1‑100 (MQ=255)
                                                                  aTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGtt                                   <  1:1405406/100‑1 (MQ=255)
                                                                  aTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGtt                                   <  2:2311932/100‑1 (MQ=255)
                                                                  aTCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGtt                                   <  2:2595499/100‑1 (MQ=255)
                                                                   tCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGttt                                  >  2:3361657/1‑100 (MQ=255)
                                                                    cacaAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttc                                 >  2:2435850/1‑100 (MQ=255)
                                                                     acaAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttct                                >  2:811050/1‑100 (MQ=255)
                                                                     acaAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttct                                >  2:1099507/1‑100 (MQ=255)
                                                                     acaAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttct                                >  1:3113168/1‑100 (MQ=255)
                                                                       aaaCTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttcttc                              >  2:2513435/1‑100 (MQ=255)
                                                                        aaCTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTcg                             <  2:996784/100‑1 (MQ=255)
                                                                        aaCTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTcg                             >  2:178260/1‑100 (MQ=255)
                                                                          cTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTcgcg                           <  1:2721162/100‑1 (MQ=255)
                                                                            gAATGCGCTGCTGCCAGTCGAGCTCCAGTGTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTGCGCTGGTCAGATCTTGTTTCTTcgcg                           <  1:3285635/98‑1 (MQ=255)
                                                                              aTGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGa                       <  2:605861/100‑1 (MQ=255)
                                                                               tGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGAt                      <  1:3115224/100‑1 (MQ=255)
                                                                               tGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGAt                      <  1:647279/100‑1 (MQ=255)
                                                                               tGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGAt                      <  2:2604601/100‑1 (MQ=255)
                                                                               tGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATATCTTCGCTGGTCAGCTCTTGTTTCTTCGCGCgg                        >  1:936722/1‑98 (MQ=255)
                                                                                gcgcTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATc                     <  2:1968011/100‑1 (MQ=255)
                                                                                gcgcTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATc                     <  2:2686685/100‑1 (MQ=255)
                                                                                gcgcTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTAAGATCTTGTTTCTTCGCGCGGAt                      >  1:3329598/1‑99 (MQ=255)
                                                                                 cgcTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCa                    >  1:1628238/1‑100 (MQ=255)
                                                                                    tgctgcCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgc                 <  1:2476912/100‑1 (MQ=255)
                                                                                       tgcCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgccgc              >  2:3331676/1‑100 (MQ=255)
                                                                                       tgcCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgccgc              <  1:824329/100‑1 (MQ=255)
                                                                                        gcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACgccgcc             >  1:1007068/1‑100 (MQ=255)
                                                                                         ccAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCa            <  1:2963748/100‑1 (MQ=255)
                                                                                          cAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCAt           >  1:704644/1‑100 (MQ=255)
                                                                                           aGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATc          <  1:47193/100‑1 (MQ=255)
                                                                                            gTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATAACGCCGCCATCt         >  2:623197/1‑100 (MQ=255)
                                                                                             tCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCtt        <  2:2806177/100‑1 (MQ=255)
                                                                                              cGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTc       >  1:789532/1‑100 (MQ=255)
                                                                                              cGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTc       >  2:365255/1‑100 (MQ=255)
                                                                                               gAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTcc      >  2:1907708/1‑100 (MQ=255)
                                                                                                 gcgcCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCAg    <  2:1297910/100‑1 (MQ=255)
                                                                                                   gcCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCAGCa  >  2:1071348/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ATATCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCAGCA  >  NC_000913/409223‑409421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: