Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 443,022 G→A 16.8% intergenic (+25/+29) thiI → / ← yajL tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913443,0220GA16.8% 345.4 / 53.7 148intergenic (+25/+29)thiI/yajLtRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base G (62/61);  new base A (15/10);  total (77/71)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

Only 100 of 157 total aligned reads displayed.

GGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCC  >  NC_000913/442923‑443121
                                                                                                   |                                                                                                   
ggggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTgg                                                                                                     <  1:1360706/100‑1 (MQ=255)
  ggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTc                                                                                                   <  1:1980321/100‑1 (MQ=255)
  ggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTc                                                                                                   <  2:1235730/100‑1 (MQ=255)
   gTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCa                                                                                                  <  2:426774/100‑1 (MQ=255)
   gTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCa                                                                                                  <  2:23920/100‑1 (MQ=255)
   gTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCa                                                                                                  >  2:3116250/1‑100 (MQ=255)
     gatgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGa                                                                                                >  1:1849028/1‑100 (MQ=255)
      atgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGAt                                                                                               >  2:3194906/1‑100 (MQ=255)
       tgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATg                                                                                              <  2:805735/100‑1 (MQ=255)
        gaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGc                                                                                             <  1:1904448/100‑1 (MQ=255)
          gCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGa                                                                                           >  1:2817304/1‑100 (MQ=255)
           ccGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGAc                                                                                          <  2:1995316/100‑1 (MQ=255)
            cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGAcn                                                                                         <  1:3301302/100‑2 (MQ=255)
            cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGACg                                                                                         <  2:1138118/100‑1 (MQ=255)
            cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGACg                                                                                         <  1:1372165/100‑1 (MQ=255)
            cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGACg                                                                                         <  1:112708/100‑1 (MQ=255)
            cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGACg                                                                                         <  1:626208/100‑1 (MQ=255)
             gTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGACGt                                                                                        <  1:880926/100‑1 (MQ=255)
                tGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTg                                                                                     >  1:2834313/1‑100 (MQ=255)
                  cAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGCGGTTTTTACGTCGCATCTGGTCAGCTGCGACGTTTGcc                                                                                   <  1:2090744/100‑1 (MQ=255)
                  cAGGCGCTCTATCAGCGCGAACAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTAGTCAGATGCGACGTTTGcc                                                                                   >  1:2018861/1‑100 (MQ=255)
                   aGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCg                                                                                  <  2:1054607/100‑1 (MQ=255)
                        ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATcc                                                                             <  2:2014713/100‑1 (MQ=255)
                        ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATcc                                                                             <  2:614561/100‑1 (MQ=255)
                         tctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCg                                                                            >  1:121267/1‑100 (MQ=255)
                           tATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGac                                                                          <  2:446215/100‑1 (MQ=255)
                            aTCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGaca                                                                         >  2:2322479/1‑100 (MQ=255)
                               tGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACacta                                                                      >  1:1919649/1‑100 (MQ=255)
                                gcgcGAGTAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACactac                                                                     >  1:1035921/1‑100 (MQ=255)
                                    gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGt                                                                 >  1:1028188/1‑100 (MQ=255)
                                    gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAAGTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGt                                                                 >  1:3186591/1‑100 (MQ=255)
                                     aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGta                                                                >  1:1696149/1‑100 (MQ=255)
                                     aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGta                                                                <  2:2539848/100‑1 (MQ=255)
                                       cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                              >  2:686695/1‑100 (MQ=255)
                                        aGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaata                                                             <  1:861767/100‑1 (MQ=255)
                                         gggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                            >  1:311659/1‑100 (MQ=255)
                                          ggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTaataat                                                           <  1:1314428/100‑1 (MQ=255)
                                          ggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTaataat                                                           >  2:2752801/1‑100 (MQ=255)
                                          ggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTaataat                                                           <  2:1843280/100‑1 (MQ=255)
                                           gCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAAtt                                                          <  1:2677560/100‑1 (MQ=255)
                                            cTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATta                                                         <  2:1749832/100‑1 (MQ=255)
                                               tAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATaa                                                      <  2:226136/100‑1 (MQ=255)
                                                 aCAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAAt                                                    >  2:856932/1‑100 (MQ=255)
                                                  cAATGTGAAGGTATATCGCCCGTAATTTGTTGTTTTTACGTCGCATCTGGTCAGATGAGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATc                                                   <  1:1868927/100‑1 (MQ=255)
                                                    aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATccc                                                 <  2:1392394/100‑1 (MQ=255)
                                                        gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgcc                                             >  2:3193436/1‑100 (MQ=255)
                                                          aGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgc                                           <  1:2700681/100‑1 (MQ=255)
                                                           ggTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                          >  2:2806644/1‑100 (MQ=255)
                                                           ggTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                          <  2:1507455/100‑1 (MQ=255)
                                                            gTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCa                                         <  1:1093557/100‑1 (MQ=255)
                                                              atatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATc                                       <  1:1264391/100‑1 (MQ=255)
                                                               tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCa                                      >  1:1528320/1‑100 (MQ=255)
                                                                atCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                     >  2:2495415/1‑100 (MQ=255)
                                                                  cGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAcc                                    >  2:2429657/1‑99 (MQ=255)
                                                                   gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAg                                  >  2:2509815/1‑100 (MQ=255)
                                                                     ccGTACTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCa                                   >  1:1006070/1‑97 (MQ=255)
                                                                     ccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGtt                                >  2:20591/1‑100 (MQ=255)
                                                                     ccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGtt                                >  2:1960282/1‑100 (MQ=255)
                                                                      cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtg                               <  1:2324579/100‑1 (MQ=255)
                                                                       gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgt                              <  2:270939/100‑1 (MQ=255)
                                                                         aaTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                            <  1:1477573/100‑1 (MQ=255)
                                                                          aTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGAt                           <  1:3139883/100‑1 (MQ=255)
                                                                           tttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                          <  2:2410027/100‑1 (MQ=255)
                                                                           tttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                          <  2:1961411/100‑1 (MQ=255)
                                                                            ttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGc                         <  2:691178/100‑1 (MQ=255)
                                                                             tgtgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                        <  2:915897/100‑1 (MQ=255)
                                                                              gtgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCa                       >  1:1780353/1‑100 (MQ=255)
                                                                              gtgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCa                       >  1:2546767/1‑100 (MQ=255)
                                                                              gtgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCa                       >  2:2829958/1‑100 (MQ=255)
                                                                                ggTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACt                     >  2:1439816/1‑100 (MQ=255)
                                                                                 gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACtt                    >  2:1593020/1‑100 (MQ=255)
                                                                                  tttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTc                   <  2:1449414/100‑1 (MQ=255)
                                                                                  tttttACGTCGAATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTTATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCAc                      <  1:1625393/97‑1 (MQ=255)
                                                                                    tttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCAt                 >  2:1375385/1‑100 (MQ=255)
                                                                                    tttACGCCTCATCTGGTCAGATGAGACGTTTGCCGCATCCGACACTTCTCGTAATAATTATAAATCCCTGCTGCCATCACCAGTTGTGATGCCACTTCAt                 <  1:3211915/100‑1 (MQ=255)
                                                                                     aaaCGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATg                >  1:3023808/3‑100 (MQ=255)
                                                                                      aaCGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATgg               >  1:3147761/2‑100 (MQ=255)
                                                                                       aCGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggg              >  2:2824066/1‑100 (MQ=255)
                                                                                       aCGTCGCATCTGACCAGATGAGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggg              >  1:2075332/1‑100 (MQ=255)
                                                                                        cGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  2:60645/1‑100 (MQ=255)
                                                                                        cGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  2:1172513/1‑100 (MQ=255)
                                                                                        cGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  1:1274246/1‑100 (MQ=255)
                                                                                         gccGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTAGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt            >  2:2183812/3‑100 (MQ=255)
                                                                                         gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  1:1714721/1‑99 (MQ=255)
                                                                                         gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt            >  2:3020701/1‑100 (MQ=255)
                                                                                         gTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggggt            >  2:1023869/1‑98 (MQ=255)
                                                                                         gTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt            >  1:1583660/1‑100 (MQ=255)
                                                                                           cGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCCCCAGTTGTGATGCCACTTCATGGGCttt          >  2:2031757/1‑100 (MQ=255)
                                                                                           cGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttt          <  2:2797379/100‑1 (MQ=255)
                                                                                            gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt         >  1:439051/1‑100 (MQ=255)
                                                                                            gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt         >  2:3155578/1‑100 (MQ=255)
                                                                                              aTCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTc       <  2:2072546/100‑1 (MQ=255)
                                                                                              aTCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTc       <  1:1632812/100‑1 (MQ=255)
                                                                                                 tGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACg    >  1:1013630/1‑100 (MQ=255)
                                                                                                 tGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACg    <  1:2495396/100‑1 (MQ=255)
                                                                                                 tGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACg    <  2:2156167/100‑1 (MQ=255)
                                                                                                  ggTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc   >  1:2988945/1‑100 (MQ=255)
                                                                                                  ggTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc   >  1:1325273/1‑100 (MQ=255)
                                                                                                  ggTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc   >  2:1624670/1‑100 (MQ=255)
                                                                                                   gTCAGATGCGACGTTTGCCGCATCCGACACTCCTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGcc  <  2:490680/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCC  >  NC_000913/442923‑443121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: