Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 459,256 T→C 51.2% D123D (GAT→GAC lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,2560TC51.2% 1.5 / 162.3 117D123D (GAT→GAClonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (25/32);  new base C (27/33);  total (52/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 120 total aligned reads displayed.

GCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGCTG  >  NC_000913/459157‑459355
                                                                                                   |                                                                                                   
gCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATtgat                                                                                                     >  2:2873946/1‑100 (MQ=255)
   ggTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAg                                                                                                  >  1:1953632/1‑100 (MQ=255)
   ggTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAg                                                                                                  >  1:1573371/1‑100 (MQ=255)
     tCGCGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACCCTTTTCTGCGACGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCg                                                                                                <  2:1000381/100‑1 (MQ=255)
      cGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCgg                                                                                               <  2:2570927/100‑1 (MQ=255)
       gAGTGGTTATAGGGTGCGCGTATTTGTGCGCTGTGTGATAATGGCGAACACTTTTCTGCGAAGGCTGGGTATCTGGAGTTGCCGACCATTGACGAGCgtg                                                                                              <  1:3220442/100‑3 (MQ=255)
       gAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCggg                                                                                              <  1:2666954/100‑1 (MQ=255)
       gAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCggg                                                                                              <  1:792578/100‑1 (MQ=255)
       gAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCggg                                                                                              <  2:2058798/100‑1 (MQ=255)
       gAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCggg                                                                                              >  2:3201195/1‑100 (MQ=255)
       gAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACCTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCggg                                                                                              <  2:3043911/100‑1 (MQ=255)
        agtggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACGCTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCgg                                                                                               <  1:3155967/95‑1 (MQ=255)
         ggggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGAGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGaa                                                                                            >  1:501804/1‑100 (MQ=255)
             ttACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAgg                                                                                        <  2:163078/100‑1 (MQ=255)
              tACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGa                                                                                       <  2:3056786/100‑1 (MQ=255)
                 aGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGt                                                                                    <  2:2438966/100‑1 (MQ=255)
                   cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGagatcg                                                                                  >  1:453631/1‑95 (MQ=255)
                   cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGc                                                                                  <  2:1595969/100‑1 (MQ=255)
                   cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGc                                                                                  <  2:792564/100‑1 (MQ=255)
                       cgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGt                                                                              <  1:1012366/100‑1 (MQ=255)
                       cgcgTATTGCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCt                                                                                 <  1:3291762/97‑1 (MQ=255)
                        gcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTg                                                                             <  2:1257969/100‑1 (MQ=255)
                        gcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTg                                                                             <  2:2097559/100‑1 (MQ=255)
                        gcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTg                                                                             >  1:2031140/1‑100 (MQ=255)
                         cgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGAGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGCACAGGAAGTGCTGGTGc                                                                            >  1:1876080/1‑100 (MQ=255)
                           tATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGt                                                                          <  2:81436/100‑1 (MQ=255)
                             tttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTAc                                                                        >  1:2518144/1‑100 (MQ=255)
                              tgcTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTAc                                                                        <  1:378103/97‑1 (MQ=255)
                                cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc                                                                     <  2:1828292/100‑1 (MQ=255)
                                cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc                                                                     <  2:1275631/100‑1 (MQ=255)
                                cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc                                                                     <  2:3046094/100‑1 (MQ=255)
                                cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc                                                                     <  2:3066485/100‑1 (MQ=255)
                                  gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa                                                                   >  2:722593/1‑100 (MQ=255)
                                  gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa                                                                   >  1:2936118/1‑100 (MQ=255)
                                    ggtctctGAAAAGGGCGATCACTTTCGTGCGAAGGCGGACTATCTGGAGGCTGCGGCCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCGAt                                                                  <  1:733756/97‑1 (MQ=255)
                                    gcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATc                                                                 <  1:656438/100‑1 (MQ=255)
                                     ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCTACCATTGATGAGCGGGAACAGGAAGTGCTGGTGAGTACTGCAATCa                                                                >  2:3377820/1‑100 (MQ=255)
                                     ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                                <  1:342247/100‑1 (MQ=255)
                                     ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                                >  2:3296691/1‑100 (MQ=255)
                                     ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                                <  2:2520308/100‑1 (MQ=255)
                                       ctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGc                                                              >  1:1252351/1‑100 (MQ=255)
                                        tctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGcc                                                             <  1:55797/100‑1 (MQ=255)
                                         ctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCa                                                            >  1:1694332/1‑100 (MQ=255)
                                          tGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAg                                                           >  1:1671663/1‑100 (MQ=255)
                                           gACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGt                                                          <  2:2310354/100‑1 (MQ=255)
                                             cAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTc                                                        <  2:2645222/100‑1 (MQ=255)
                                                tGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAACCAGCCAGTTCgga                                                     <  2:2299360/100‑3 (MQ=255)
                                                 ggCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAg                                                    >  1:2266725/1‑100 (MQ=255)
                                                 ggCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAg                                                    >  1:1083143/1‑100 (MQ=255)
                                                 ggCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAg                                                    <  2:2601596/100‑1 (MQ=255)
                                                 ggCGAACACTTGTCTGCGAAGGCGGAGTAGCTGGAGTCGCCGACCATTGACGAGCGGGAGCAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAg                                                    <  1:1409537/100‑1 (MQ=255)
                                                  gCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAgg                                                   <  2:2936143/100‑1 (MQ=255)
                                                  gCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAgg                                                   <  2:3161888/100‑1 (MQ=255)
                                                  gCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGGGCTGGTGCGTACTGCAATCAGCCAGTGCTAAgg                                                   >  2:1803016/1‑100 (MQ=255)
                                                   cGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGc                                                  <  1:1044291/100‑1 (MQ=255)
                                                   cGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGc                                                  <  2:1763708/100‑1 (MQ=255)
                                                   cGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGc                                                  <  1:2427213/100‑1 (MQ=255)
                                                    gAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCAGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCt                                                 >  1:1812280/1‑100 (MQ=255)
                                                       cacTTTTCTGCGAAGGTGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACa                                              <  1:1981986/100‑1 (MQ=255)
                                                       cacTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACa                                              >  1:320562/1‑100 (MQ=255)
                                                        agttttCTGCGAAGGCTGAGTAGCTGGAGTCGCCGGCCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACAt                                             <  2:622714/98‑1 (MQ=255)
                                                         cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc                                            >  1:1729010/1‑100 (MQ=255)
                                                         cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc                                            >  1:2615934/1‑100 (MQ=255)
                                                         cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc                                            <  2:443829/100‑1 (MQ=255)
                                                         cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc                                            >  1:948216/1‑100 (MQ=255)
                                                          ttttCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCa                                           >  1:2988404/1‑100 (MQ=255)
                                                          ttttCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCa                                           >  2:3098147/1‑100 (MQ=255)
                                                           tttCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTAAATCaa                                          >  1:9353/1‑100 (MQ=255)
                                                             tCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGc                                        <  2:1489888/100‑1 (MQ=255)
                                                               tGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTg                                      >  2:1867672/1‑100 (MQ=255)
                                                               tGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTg                                      >  2:1825124/1‑100 (MQ=255)
                                                               tGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTg                                      >  1:442139/1‑100 (MQ=255)
                                                                  gAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAAc                                   <  2:754240/100‑1 (MQ=255)
                                                                  gAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAAc                                   <  2:1047610/100‑1 (MQ=255)
                                                                     ggCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaa                                >  1:1747872/1‑100 (MQ=255)
                                                                      gCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaa                               <  2:1674177/100‑1 (MQ=255)
                                                                       cGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaaa                              >  1:301284/1‑100 (MQ=255)
                                                                           gTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATc                          <  2:2461663/100‑1 (MQ=255)
                                                                           gTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATc                          <  1:2496354/100‑1 (MQ=255)
                                                                             aTCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc                        >  1:1297371/1‑100 (MQ=255)
                                                                             aTCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc                        >  1:1629374/1‑100 (MQ=255)
                                                                              tCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCcca                       >  2:1334339/1‑100 (MQ=255)
                                                                              tCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCcca                       <  2:649541/100‑1 (MQ=255)
                                                                              tCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCcca                       >  1:2507582/1‑100 (MQ=255)
                                                                               cTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccac                      >  1:823365/1‑100 (MQ=255)
                                                                                tGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc                     <  2:2485857/100‑1 (MQ=255)
                                                                                tGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc                     >  1:1226279/1‑100 (MQ=255)
                                                                                tGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc                     <  2:570253/100‑1 (MQ=255)
                                                                                 ggAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacca                    >  1:906868/1‑100 (MQ=255)
                                                                                  gAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAg                   <  1:1373173/100‑1 (MQ=255)
                                                                                  gAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAg                   <  2:1496155/100‑1 (MQ=255)
                                                                                      cGCAGACCATTGACGAGCGGGAACAGGAAGTGGTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATAAAGCTGAAAAAAAAAATCCCACCAGAAg                <  1:1988960/99‑1 (MQ=255)
                                                                                       gCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTg              >  2:3140288/1‑100 (MQ=255)
                                                                                       gCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTg              <  2:3116647/100‑1 (MQ=255)
                                                                                            ccATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc         >  2:589587/1‑100 (MQ=255)
                                                                                            ccATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc         <  2:1532020/100‑1 (MQ=255)
                                                                                             cATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACg        >  2:758123/1‑100 (MQ=255)
                                                                                               ttGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTc      >  2:536572/1‑100 (MQ=255)
                                                                                                 gatgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGc    <  2:2266699/100‑1 (MQ=255)
                                                                                                   tgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTCAACAAAAAAATCCCACCAGAAGTGCTGACGTCGCTg  <  1:3177393/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCGCTG  >  NC_000913/459157‑459355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: