Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 532,380 A→G 64.8% intergenic (‑159/‑71) allS ← / → allA allD operon transcriptional activator/ureidoglycolate lyase, releasing urea

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913532,3800AG64.8% 139.7 / 125.2 145intergenic (‑159/‑71)allS/allAallD operon transcriptional activator/ureidoglycolate lyase, releasing urea
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base G (33/60);  total (57/88)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

Only 100 of 147 total aligned reads displayed.

AGTATTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTAAGT  >  NC_000913/532281‑532477
                                                                                                   |                                                                                                 
aGTATTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGa                                                                                                   >  2:3311297/1‑100 (MQ=255)
aGTATTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGa                                                                                                   >  1:1234955/1‑100 (MQ=255)
   aTTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTg                                                                                                <  2:1803926/100‑1 (MQ=255)
    tttttACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTAATGTTGTTCTCTTAAGGTTTTAGATTGc                                                                                               <  2:415420/100‑1 (MQ=255)
    tttttACATGATAACCCAGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGc                                                                                               <  1:810804/100‑1 (MQ=255)
     ttttACAGTATAACCCTGTTGAACTTGTGGACTAAATCTAGTTTTTGAAAAATAGTCGTAGGTTAGTATTGATGATGTACTCTTCAGGTTTTAGGTGGcc                                                                                              <  1:3061684/100‑1 (MQ=255)
     tgtgaCATGATAACGCTGTTCAATTTGTGGATTAAATCTAGGTTTGGAAAAATATTCCAACTTTTGTATTTATGTTGTTCTCTTAAGGTTTTGGGTTGcc                                                                                              <  1:3131930/96‑1 (MQ=255)
      tttACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGcc                                                                                              <  1:2393955/99‑1 (MQ=255)
      tttACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGAAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGcc                                                                                              <  1:2301037/99‑1 (MQ=255)
        tACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGt                                                                                           >  1:1068193/1‑100 (MQ=255)
        tACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGt                                                                                           >  2:482153/1‑100 (MQ=255)
          cATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTa                                                                                         <  1:2630852/100‑1 (MQ=255)
           aTGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTAt                                                                                        <  2:778040/100‑1 (MQ=255)
            tGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTAtt                                                                                       >  2:2365822/1‑100 (MQ=255)
               tAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGaa                                                                                    <  1:2566302/100‑1 (MQ=255)
               tAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGaa                                                                                    <  2:523504/100‑1 (MQ=255)
                 aCCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAc                                                                                  >  1:1047463/1‑100 (MQ=255)
                 aCCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAc                                                                                  <  2:610481/100‑1 (MQ=255)
                 aCCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAc                                                                                  >  1:1521495/1‑100 (MQ=255)
                     ttttCAATTTGTGGATTAAATCTAGTTTTGGAACAATATTTCAACTTTTGTATTTATGTTTTTCTCTTCAGGTTTTAGGTTGCCTGTTATTGAATCCAAg                                                                              <  1:3026284/98‑1 (MQ=255)
                      gTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGc                                                                             <  1:2421538/100‑1 (MQ=255)
                       ttCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCt                                                                            >  1:2613017/1‑100 (MQ=255)
                           aTTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGAcc                                                                        <  2:442614/100‑1 (MQ=255)
                           aTTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGAcc                                                                        <  1:3158537/100‑1 (MQ=255)
                            tttGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCg                                                                       <  1:1569997/100‑1 (MQ=255)
                            tttGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCg                                                                       >  1:1353204/1‑100 (MQ=255)
                            tttGTAGACTAAATCTATTTTTGTAAAAATATTCCAATTTTTGTATTGATGCTGGTCTCTTAAGGTTTTACGTTGCCTCTTATTGAAACCAAGCTGAcc                                                                        <  1:1896247/99‑1 (MQ=255)
                             ttGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCgg                                                                      <  2:1646291/100‑1 (MQ=255)
                              tgtgGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGt                                                                     >  1:1343086/1‑100 (MQ=255)
                                tgGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTcg                                                                   <  2:2509187/100‑1 (MQ=255)
                                tgGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTcg                                                                   >  1:352363/1‑100 (MQ=255)
                                 ggACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTcgg                                                                  <  2:2866966/100‑1 (MQ=255)
                                  gACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTcggc                                                                 >  2:3140932/1‑100 (MQ=255)
                                      aaaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtg                                                             >  2:1598287/1‑100 (MQ=255)
                                       aaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtgg                                                            <  1:2808106/100‑1 (MQ=255)
                                       aaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtgg                                                            <  1:3338253/100‑1 (MQ=255)
                                       aaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGATCGGTCGGCggtgg                                                            <  1:1655533/100‑1 (MQ=255)
                                       aaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtgg                                                            <  1:412242/100‑1 (MQ=255)
                                       aaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtgg                                                            <  2:1990837/100‑1 (MQ=255)
                                       aaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtgg                                                            <  1:1401813/100‑1 (MQ=255)
                                          cTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTg                                                         >  1:1803746/1‑100 (MQ=255)
                                          cTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTg                                                         <  1:244199/100‑1 (MQ=255)
                                           tAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTGCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTAGGCGGTGGTTGa                                                        >  1:2012410/1‑100 (MQ=255)
                                             gTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAAc                                                      <  2:3228604/100‑1 (MQ=255)
                                             gTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAAc                                                      >  2:1895328/1‑100 (MQ=255)
                                              ttttGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACg                                                     <  1:2177559/100‑1 (MQ=255)
                                              ttttGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACg                                                     <  1:1753709/100‑1 (MQ=255)
                                                  ggAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCTGTCGGCGGTGGTTGAACGGAAt                                                 >  1:624097/1‑100 (MQ=255)
                                                  ggAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGaa                                                  >  1:1308809/1‑99 (MQ=255)
                                                   gAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAAtt                                                >  2:2647414/1‑100 (MQ=255)
                                                    aaaaaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAATGGAATTa                                               <  2:1771173/100‑1 (MQ=255)
                                                    aaaaaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTa                                               <  2:82331/100‑1 (MQ=255)
                                                    aaaaaTATTCCAACTTTTGTATTGATGTTGTGCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCGGACCGGTCGGCGGTGGTTGAACGGAATTa                                               <  1:869793/100‑1 (MQ=255)
                                                     aaaaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTAt                                              >  1:2324156/1‑100 (MQ=255)
                                                        atatTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTa                                               <  1:1042733/96‑1 (MQ=255)
                                                         tatTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTa                                          <  2:171221/100‑1 (MQ=255)
                                                         tatTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTa                                          <  2:1330811/100‑1 (MQ=255)
                                                          atTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTAc                                         >  1:654800/1‑100 (MQ=255)
                                                            tCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACaa                                       >  2:2453982/1‑100 (MQ=255)
                                                             ccAACTTTTGTATTGCTGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAg                                      <  2:3333552/100‑1 (MQ=255)
                                                             ccAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAg                                      >  1:2359186/1‑100 (MQ=255)
                                                             ccAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAg                                      >  2:2585773/1‑100 (MQ=255)
                                                             ccAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAg                                      <  2:934196/100‑1 (MQ=255)
                                                             ccAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAg                                      >  1:2658675/1‑100 (MQ=255)
                                                              cAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAgg                                     >  1:908867/1‑100 (MQ=255)
                                                              cAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAgg                                     <  2:268141/100‑1 (MQ=255)
                                                                aCTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGAc                                   >  1:1412423/1‑100 (MQ=255)
                                                                aCTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGAc                                   <  1:62307/100‑1 (MQ=255)
                                                                 cTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACa                                  >  2:723695/1‑100 (MQ=255)
                                                                 cTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACa                                  <  2:1068196/100‑1 (MQ=255)
                                                                     tGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACaaaaa                              <  1:1791051/100‑1 (MQ=255)
                                                                        aTTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAagag                             >  1:3266849/1‑98 (MQ=255)
                                                                         ttGATGTTGTTCTCTTAAGGTTTTAGTTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATg                          <  1:911635/100‑1 (MQ=255)
                                                                         ttGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATg                          <  1:1598280/100‑1 (MQ=255)
                                                                         ttGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATg                          <  1:1953835/100‑1 (MQ=255)
                                                                           gATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGaa                        >  2:1553172/1‑100 (MQ=255)
                                                                           gATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGaa                        <  1:3244566/100‑1 (MQ=255)
                                                                           gATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGaa                        <  2:1364531/100‑1 (MQ=255)
                                                                           gATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGaa                        >  1:317542/1‑100 (MQ=255)
                                                                              gttgttCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACt                     >  2:1800757/1‑100 (MQ=255)
                                                                                tgtgCTCGTAAGGTTTTATGTGGTAAGATATTGAAACCAAGCTGACAGGGTGGTGGTGGTTGAACGGATTTATGTAAAAAAAAAAAAAAGATGaa                        <  1:1663070/95‑1 (MQ=255)
                                                                                 gttCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCa                  >  1:2455196/1‑100 (MQ=255)
                                                                                 gttCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCa                  >  1:567207/1‑100 (MQ=255)
                                                                                  ttCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAg                 <  2:1187003/100‑1 (MQ=255)
                                                                                   tctcTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAgg                <  1:1677998/100‑1 (MQ=255)
                                                                                   tctcTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAgg                <  2:3105558/100‑1 (MQ=255)
                                                                                   tctcTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAgg                <  2:425921/100‑1 (MQ=255)
                                                                                      ctTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGTCTAGTCGGCGGTGGTTGTGCGGAACTAAGTTACCAGGACAAACAGGTGAAGCTTCAGGTa              >  1:2082027/1‑99 (MQ=255)
                                                                                       ttAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTAtt            >  2:3074301/1‑100 (MQ=255)
                                                                                       ttAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTAtt            <  2:2698488/100‑1 (MQ=255)
                                                                                        tAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGTGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTa           >  2:1888527/1‑100 (MQ=255)
                                                                                         aaGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTAc          <  1:3055874/100‑1 (MQ=255)
                                                                                          aGGTTTTAGGGTGCCTGTTATTGCAACCAGGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTAcc         <  1:658157/100‑1 (MQ=255)
                                                                                           ggTTTTAGATTGCTTTTTATTTAAACTAAACTTACCGGTCGGCGGTGGTTGAACTGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTAcc         <  1:621113/99‑1 (MQ=255)
                                                                                            gTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGt       >  2:1512794/1‑100 (MQ=255)
                                                                                             ttttAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGtt      <  1:482151/100‑1 (MQ=255)
                                                                                              tttAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTa     <  2:2462224/100‑1 (MQ=255)
                                                                                               ttAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTaa    >  2:3013940/1‑100 (MQ=255)
                                                                                                tAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTAAg   >  2:152573/1‑100 (MQ=255)
                                                                                                 aGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTAAGt  >  1:3292074/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
AGTATTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTAAGT  >  NC_000913/532281‑532477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: