Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 548,374 G→A 17.2% A6A (GCG→GCA ylbE → NO‑induced DUF1116 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913548,3740GA17.2% 409.1 / 66.6 175A6A (GCG→GCAylbENO‑induced DUF1116 protein
Reads supporting (aligned to +/- strand):  ref base G (72/72);  new base A (13/17);  total (85/90)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 183 total aligned reads displayed.

TGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC  >  NC_000913/548275‑548473
                                                                                                   |                                                                                                   
tgtgCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGgcg                                                                                                     <  1:3260452/100‑1 (MQ=255)
   gCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGTACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCaa                                                                                                  >  1:2333954/1‑100 (MQ=255)
    cATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAg                                                                                                 <  2:2983708/100‑1 (MQ=255)
    cATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAg                                                                                                 <  2:1920554/100‑1 (MQ=255)
     aTTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGc                                                                                                <  2:1577803/100‑1 (MQ=255)
      ttACCAATTGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATACGGGGTTCCCATGTTTACATCAGTGGCGCAAGcc                                                                                               <  1:1457535/100‑1 (MQ=255)
      ttACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGcc                                                                                               <  1:2931622/100‑1 (MQ=255)
      ttACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGcc                                                                                               <  2:1825129/100‑1 (MQ=255)
       tACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCa                                                                                              >  1:2008057/1‑100 (MQ=255)
        aCCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTCTTAGAACGTTTGCAATAAGGGGGTCCCATGTTTACATCAGTGGCGCAAGTCaa                                                                                             >  1:68473/1‑100 (MQ=255)
        aCCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCaa                                                                                             >  1:2522090/1‑100 (MQ=255)
         ccAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAAt                                                                                            >  2:329501/1‑100 (MQ=255)
         ccAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAAt                                                                                            <  1:2760198/100‑1 (MQ=255)
         ccAATGGGCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAAt                                                                                            >  2:1779209/1‑100 (MQ=255)
          cAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAAtg                                                                                           >  1:2920712/1‑100 (MQ=255)
           aaTGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAAtgc                                                                                          <  2:2652150/100‑1 (MQ=255)
           aaTGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAAtgc                                                                                          <  1:2135714/100‑1 (MQ=255)
           aaTGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAAtgc                                                                                          <  2:1565856/100‑1 (MQ=255)
           aaTGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAAtgc                                                                                          <  2:842250/100‑1 (MQ=255)
               gTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAATCCATTGCTGCg                                                                                      <  1:1779198/100‑1 (MQ=255)
               gTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCg                                                                                      <  1:1727829/100‑1 (MQ=255)
               gTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCg                                                                                      <  1:1392896/100‑1 (MQ=255)
               gTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCg                                                                                      >  2:3125464/1‑100 (MQ=255)
                tCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCgg                                                                                     <  2:2740826/100‑1 (MQ=255)
                tCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCg                                                                                      <  2:1087779/99‑1 (MQ=255)
                 cGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGt                                                                                    <  1:1303834/100‑1 (MQ=255)
                  gCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGtt                                                                                   >  1:3030591/1‑100 (MQ=255)
                  gCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGGTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCgg                                                                                     <  1:1407921/98‑1 (MQ=255)
                   ccAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTCGCAATAAGGGGTTCCGATGTTTCAATCAGTGGCGCAAGCCAATGCTGTGGtt                                                                                   >  2:2658424/1‑99 (MQ=255)
                     aGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATc                                                                                <  2:1652410/100‑1 (MQ=255)
                     aGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATc                                                                                >  1:2946251/1‑100 (MQ=255)
                     aGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATc                                                                                <  2:2946277/100‑1 (MQ=255)
                     aGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATc                                                                                <  1:836100/100‑1 (MQ=255)
                       tCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTGCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGa                                                                              <  2:774131/100‑1 (MQ=255)
                         gCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAAc                                                                            <  1:901972/100‑1 (MQ=255)
                         gCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAAc                                                                            <  2:419682/100‑1 (MQ=255)
                          ccGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACa                                                                           >  2:278147/1‑100 (MQ=255)
                                 cAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATtcgt                                                                    >  2:1275358/1‑100 (MQ=255)
                                 cAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATtcgt                                                                    >  2:91042/1‑100 (MQ=255)
                                  aaTAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAAt                                                                        <  1:3335458/95‑1 (MQ=255)
                                  aaTAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATtcgtc                                                                   <  2:82343/100‑1 (MQ=255)
                                   aTAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAAAGCTGCGGTTATCGAACAAATtcgtcg                                                                  <  2:2057467/100‑1 (MQ=255)
                                   aTAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATtcgtcg                                                                  >  1:1235222/1‑100 (MQ=255)
                                    tAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTcgc                                                                 <  2:1421840/100‑1 (MQ=255)
                                     aaaaaaCTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTcgc                                                                 >  1:3101914/1‑99 (MQ=255)
                                       aaaaCTGGCTCGTGTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCt                                                              <  1:1368282/100‑1 (MQ=255)
                                           cTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTc                                                          <  2:2920736/100‑1 (MQ=255)
                                            tGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTcc                                                         <  1:1710853/100‑1 (MQ=255)
                                             ggCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGTTTATCGAACAAATTCGTCGCGCTCGTCca                                                        >  2:2267938/1‑100 (MQ=255)
                                               cTCGTTTATTGAGACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTcc                                                         <  1:2653809/97‑1 (MQ=255)
                                               cTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCcaca                                                      >  1:2298982/1‑100 (MQ=255)
                                               cTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCcaca                                                      >  2:644974/1‑100 (MQ=255)
                                                 cGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACAct                                                    >  1:879843/1‑100 (MQ=255)
                                                  gTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACActg                                                   >  2:2482653/1‑100 (MQ=255)
                                                  gTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACActg                                                   <  1:1197420/100‑1 (MQ=255)
                                                    ttattaGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACActggc                                                 >  2:1034320/1‑100 (MQ=255)
                                                      attaGCACGTTTGCAATAAAGGATTCCCATGTTTAAATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACActggct                                                >  1:1653487/1‑99 (MQ=255)
                                                       ttaGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACActggctgg                                              >  2:1985930/1‑100 (MQ=255)
                                                        taGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGa                                             >  1:1025851/1‑100 (MQ=255)
                                                        taGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGa                                             <  2:2118183/100‑1 (MQ=255)
                                                          gaccGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGAtg                                           <  1:2453654/97‑1 (MQ=255)
                                                          gAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGAtg                                           <  2:1703659/100‑1 (MQ=255)
                                                          gAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGAtg                                           <  2:3388337/100‑1 (MQ=255)
                                                            aCGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGAtgtg                                         <  2:1148498/100‑1 (MQ=255)
                                                             ccgatctaaTAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGc                                        <  1:3194651/93‑1 (MQ=255)
                                                             cGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGc                                        <  2:3386729/100‑1 (MQ=255)
                                                              gTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCa                                       <  2:2459243/100‑1 (MQ=255)
                                                              gTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCa                                       <  2:836960/100‑1 (MQ=255)
                                                              gTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCa                                       >  1:881060/1‑100 (MQ=255)
                                                                ttGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAAc                                     >  2:2160009/1‑100 (MQ=255)
                                                                  gCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCTTCGCGCTCGTCCACACTGGCTGGAGGTGCAACCg                                   <  1:2150044/100‑1 (MQ=255)
                                                                  gCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCTTCCACACTGGCTGGATGTGCAACCg                                   <  1:186886/100‑1 (MQ=255)
                                                                   cAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCgg                                  >  2:2358904/1‑100 (MQ=255)
                                                                   cAATAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCgg                                  <  2:2434279/100‑1 (MQ=255)
                                                                    aaTAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGc                                 <  2:2522110/100‑1 (MQ=255)
                                                                     aTAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGct                                >  2:83639/1‑100 (MQ=255)
                                                                     aTAAGGGGTTCCCATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGct                                >  2:1272039/1‑100 (MQ=255)
                                                                       aaGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGACGCGCTCGTCCACACTGGCTGGATGTGCAACCGGcttc                              <  1:2082949/100‑1 (MQ=255)
                                                                         ggggTTCCCATGTTTGCATCAGTGGCACAAGCCGATGCTGCGGTTATCGAACAAATGCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGcttct                             <  2:527685/99‑1 (MQ=255)
                                                                          gggTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCttcttc                           <  2:368143/100‑1 (MQ=255)
                                                                          gggTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCttcttc                           >  2:3055513/1‑100 (MQ=255)
                                                                             ttCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACt                        <  1:4318/100‑1 (MQ=255)
                                                                             ttCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACt                        >  1:2786972/1‑100 (MQ=255)
                                                                              tCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACtt                       <  2:2854151/100‑1 (MQ=255)
                                                                               cccATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTa                      >  2:855110/1‑100 (MQ=255)
                                                                               cccATGTTTACATCAGTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTa                      <  2:539453/100‑1 (MQ=255)
                                                                                ccATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTAt                     >  1:2819946/1‑100 (MQ=255)
                                                                                 cATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTTCCTTATc                    >  1:1041327/1‑100 (MQ=255)
                                                                                 cATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATc                    >  2:3375094/1‑100 (MQ=255)
                                                                                 cATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATc                    <  2:1206640/100‑1 (MQ=255)
                                                                                   tGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAg                  >  2:508122/1‑100 (MQ=255)
                                                                                     tttACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCg                <  2:879846/100‑1 (MQ=255)
                                                                                             aGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAAc        >  2:2618098/1‑100 (MQ=255)
                                                                                             aGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAAc        >  2:3175970/1‑100 (MQ=255)
                                                                                              gTGGCACAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACg       >  1:1102392/1‑100 (MQ=255)
                                                                                                ggCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAg     >  1:2684772/1‑100 (MQ=255)
                                                                                                ggCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAg     >  2:3207678/1‑100 (MQ=255)
                                                                                                   gcAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCCGCGAACTAAACGAgg    >  1:96882/1‑98 (MQ=255)
                                                                                                   gcAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc  <  1:2866761/100‑1 (MQ=255)
                                                                                                   gcAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc  >  1:800343/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGC  >  NC_000913/548275‑548473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: