BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	662,636	T→G	62.2%	T3P (ACC→CCC)	ybeD ←	UPF0250 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,636	0	T	G	62.2%	131.3 / 126.9	122	T3P (ACC→CCC)	ybeD	UPF0250 family protein
Reads supporting (aligned to +/- strand): ref base T (21/25); new base G (36/39); total (57/65)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACT  >  NC_000913/662537‑662735

  aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGttt                                                                                                   <  1:1289505/100‑1 (MQ=255)

  aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGttt                                                                                                   >  2:1767945/1‑100 (MQ=255)

   ccACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGACTTCAAGCAGTTCGTTAAGTTTGGgttt                                                                                                  >  1:359929/1‑100 (MQ=255)

   ccACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGgttt                                                                                                  >  1:2970614/1‑100 (MQ=255)

    cACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTc                                                                                                 >  1:2735844/1‑100 (MQ=255)

     aCTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCa                                                                                                <  2:2716716/100‑1 (MQ=255)

     aCTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCa                                                                                                >  1:2716694/1‑100 (MQ=255)

      cTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCAt                                                                                               >  1:2670821/1‑100 (MQ=255)

       ttCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATg                                                                                              <  2:673731/100‑1 (MQ=255)

       ttCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATg                                                                                              >  2:215350/1‑100 (MQ=255)

       ttCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATg                                                                                              <  2:3256926/100‑1 (MQ=255)

        tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGt                                                                                             >  2:409392/1‑100 (MQ=255)

         cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTc                                                                                            <  2:436715/100‑1 (MQ=255)

           accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAg                                                                                          <  2:1756208/100‑1 (MQ=255)

           accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAg                                                                                          >  1:46530/1‑100 (MQ=255)

           accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAg                                                                                          <  1:85012/100‑1 (MQ=255)

           acaaccTGATAAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAATCAGTTCGTTAAGTTTTGGTTTCATGTCAg                                                                                          <  2:630774/97‑1 (MQ=255)

            ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGc                                                                                         <  2:62235/100‑1 (MQ=255)

            ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGc                                                                                         <  2:585388/100‑1 (MQ=255)

               ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTcc                                                                                      >  1:3344372/1‑100 (MQ=255)

                cTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCg                                                                                     >  1:2623266/1‑100 (MQ=255)

                    tCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGcg                                                                                  >  1:2068669/1‑99 (MQ=255)

                       aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGcgtaac                                                                              >  1:1188578/1‑100 (MQ=255)

                       aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaac                                                                              >  2:181535/1‑100 (MQ=255)

                       aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaac                                                                              <  1:2993051/100‑1 (MQ=255)

                         cAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGcgtaacgt                                                                            <  1:215350/100‑1 (MQ=255)

                         cAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGcgtaacgt                                                                            >  2:2617291/1‑100 (MQ=255)

                            cTCAGGTAACGCCTGCCCCATACCTTTGTAAGTAAAAGGAGGAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGcgtaacgtaa                                                                          >  1:2479159/1‑99 (MQ=255)

                            cTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAAt                                                                         <  2:19256/100‑1 (MQ=255)

                             tCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAAtt                                                                        <  1:2347352/100‑1 (MQ=255)

                              cAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTa                                                                       <  2:553259/100‑1 (MQ=255)

                               aGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTaa                                                                      <  2:2157321/100‑1 (MQ=255)

                                ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTaaa                                                                     >  1:1094188/1‑100 (MQ=255)

                                ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTaaa                                                                     >  2:2265373/1‑100 (MQ=255)

                                 gTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAAt                                                                    >  1:234730/1‑100 (MQ=255)

                                 gTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAAt                                                                    <  1:3368004/100‑1 (MQ=255)

                                  tAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATa                                                                   <  1:1038519/100‑1 (MQ=255)

                                    aCGCCTGTCCCATAACTTTGTAAGTAAAAGGGGTCGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGc                                                                 <  1:1778040/100‑1 (MQ=255)

                                    aCGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGc                                                                 <  2:2692712/100‑1 (MQ=255)

                                     cGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCa                                                                <  1:2152023/100‑1 (MQ=255)

                                       ccTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAAc                                                              <  2:2546314/100‑1 (MQ=255)

                                       ccTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTAGGTTTTCATGTCAGCTCCGGGGTAACGTAATAAAATAGCAAc                                                              >  1:1585896/1‑100 (MQ=255)

                                        ctgcaacATAACTTTGTAAGTAAAAGGAGTAGGGAATTAAAGCAGTTCGTAAAGTGTGGGTTTCATGTCAGCACAGGGGTAACGTAATGAAATAGCAAc                                                              >  1:1351873/7‑99 (MQ=255)

                                        cTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACt                                                             >  1:1658335/1‑100 (MQ=255)

                                        cTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACt                                                             <  2:1706105/100‑1 (MQ=255)

                                          gCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTcc                                                           <  2:1952661/100‑1 (MQ=255)

                                          gCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTcc                                                           >  1:3065609/1‑100 (MQ=255)

                                          gCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTcc                                                           <  1:747895/100‑1 (MQ=255)

                                           ccccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTCACGTAATTAAATAGCAACTccc                                                          >  2:3062918/1‑100 (MQ=255)

                                            cccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCACCTccc                                                          <  1:571188/99‑1 (MQ=255)

                                            cccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTccc                                                          >  2:538263/1‑99 (MQ=255)

                                            cccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCg                                                         >  2:37890/1‑100 (MQ=255)

                                             ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGc                                                        >  1:910084/1‑100 (MQ=255)

                                             ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGc                                                        >  2:1362070/1‑100 (MQ=255)

                                              cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGcc                                                       <  2:859295/100‑1 (MQ=255)

                                                tAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAg                                                     >  1:3179102/1‑100 (MQ=255)

                                                 aaCTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGa                                                    >  2:2336289/1‑100 (MQ=255)

                                                   cTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTACTAAACTAGCAACTCCCGACACAAg                                                  >  2:2320228/1‑100 (MQ=255)

                                                   cTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAg                                                  <  2:2878631/100‑1 (MQ=255)

                                                     ttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGc                                                >  2:2092329/1‑100 (MQ=255)

                                                       gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCgg                                              <  2:2436250/100‑1 (MQ=255)

                                                       gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGccttctggcgg                                              <  2:2970640/100‑10 (MQ=255)

                                                         aagtcaAAGGAGTAGGGAATACACGCAGTTCGTGAAGGCTGGGATTCAGGTCAGAACCGGCGTAACGCAATTAAATAGCAACTCCCGCCACAAGGCCGGa                                            >  1:2346772/1‑100 (MQ=255)

                                                         aagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGa                                            <  1:100334/100‑1 (MQ=255)

                                                          agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                           <  1:2813067/100‑1 (MQ=255)

                                                           gtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGt                                          >  2:1536664/1‑100 (MQ=255)

                                                           gtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAg                                           >  2:3379529/1‑99 (MQ=255)

                                                            taaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtt                                         >  2:1024975/1‑100 (MQ=255)

                                                             aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAGt                                          <  1:2451043/98‑1 (MQ=255)

                                                             aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttt                                        <  2:815731/100‑1 (MQ=255)

                                                               aaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                      <  1:3232005/100‑1 (MQ=255)

                                                                aGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttttt                                     <  1:1173423/100‑1 (MQ=255)

                                                                  gAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTAc                                   <  1:1280435/100‑1 (MQ=255)

                                                                  gAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTAc                                   <  2:2633794/100‑1 (MQ=255)

                                                                   aGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACt                                  <  2:147805/100‑1 (MQ=255)

                                                                   aGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACt                                  <  2:1578001/100‑1 (MQ=255)

                                                                   aGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACt                                  <  1:1918571/100‑1 (MQ=255)

                                                                    gTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTg                                 <  1:37890/100‑1 (MQ=255)

                                                                      aGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAt                               >  1:3278569/1‑100 (MQ=255)

                                                                      aGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAt                               >  2:1487374/1‑100 (MQ=255)

                                                                      aGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAt                               >  2:379434/1‑100 (MQ=255)

                                                                        ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGc                             >  2:1462627/1‑100 (MQ=255)

                                                                        ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGc                             <  2:1250326/100‑1 (MQ=255)

                                                                          aaTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCtt                           <  2:1519086/100‑1 (MQ=255)

                                                                            tcttccgatctCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAg                         <  1:879446/90‑1 (MQ=255)

                                                                             tCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGt                        >  1:82361/1‑100 (MQ=255)

                                                                             tCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCGCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGt                        <  1:2894357/100‑1 (MQ=255)

                                                                              cAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                       >  1:717014/1‑100 (MQ=255)

                                                                              cAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                       >  1:735458/1‑100 (MQ=255)

                                                                              cAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                       <  2:1423231/100‑1 (MQ=255)

                                                                              cAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                       >  1:2324465/1‑100 (MQ=255)

                                                                                   aGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgg                  <  1:1536657/100‑1 (MQ=255)

                                                                                    gTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg                 <  1:3336967/100‑1 (MQ=255)

                                                                                       cGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAc              >  2:318139/1‑100 (MQ=255)

                                                                                        gTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGa               >  1:1750497/1‑98 (MQ=255)

                                                                                             gTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAt        >  2:944460/1‑100 (MQ=255)

                                                                                             gTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAt        >  2:1984872/1‑100 (MQ=255)

                                                                                              tttGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAtt       <  1:1599839/100‑1 (MQ=255)

                                                                                               ttGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa      >  2:2312031/1‑100 (MQ=255)

                                                                                                   ttttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACt  >  1:3117803/1‑100 (MQ=255)

GTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACT  >  NC_000913/662537‑662735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`