BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	674,180	G→A	21.7%	L202L (CTG→TTG)	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,180	0	G	A	21.7%	284.7 / 71.8	138	L202L (CTG→TTG)	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (51/57); new base A (21/9); total (72/66)
Fisher's exact test for biased strand distribution p-value = 3.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 148 total aligned reads displayed.

AAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTC  >  NC_000913/674083‑674279

aaGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCt                                                                                                    <  1:3380622/100‑1 (MQ=255)

 aGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTc                                                                                                   >  1:2980432/1‑100 (MQ=255)

  ggTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCg                                                                                                  <  2:1661070/100‑1 (MQ=255)

   gTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCg                                                                                                  >  1:1678165/1‑99 (MQ=255)

     gATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTc                                                                                                <  1:1947201/99‑1 (MQ=255)

     gATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCa                                                                                               >  2:306720/1‑100 (MQ=255)

      aTCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAg                                                                                              >  2:1449978/1‑100 (MQ=255)

      aTCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAg                                                                                              <  2:2131655/100‑1 (MQ=255)

       tctcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGc                                                                                             >  1:3022211/1‑100 (MQ=255)

       tctcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGc                                                                                             >  1:263605/1‑100 (MQ=255)

        ctcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCg                                                                                            <  1:3286631/100‑1 (MQ=255)

        ctcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCg                                                                                            >  2:1719974/1‑100 (MQ=255)

         tcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGt                                                                                           >  2:2608740/1‑100 (MQ=255)

         tcCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGt                                                                                           <  2:2684814/100‑1 (MQ=255)

          ccACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTa                                                                                          >  2:2975943/1‑100 (MQ=255)

          ccACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTa                                                                                          <  1:2477920/100‑1 (MQ=255)

             cGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGc                                                                                       <  2:648326/100‑1 (MQ=255)

             cGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGc                                                                                       >  2:567748/1‑100 (MQ=255)

             cGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGc                                                                                       >  2:2001854/1‑100 (MQ=255)

             cGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGc                                                                                       >  2:2567825/1‑100 (MQ=255)

                  tCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGa                                                                                  >  1:63283/1‑100 (MQ=255)

                   cGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑t                                                                                >  1:594210/1‑100 (MQ=255)

                    ggAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑tt                                                                               >  1:1730403/1‑100 (MQ=255)

                    ggAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑tt                                                                               >  2:585982/1‑100 (MQ=255)

                    ggAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑tt                                                                               >  2:1055531/1‑100 (MQ=255)

                    ggAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑tt                                                                               >  2:1504982/1‑100 (MQ=255)

                      aaCGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑tttt                                                                             <  1:2249380/100‑1 (MQ=255)

                        cGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGa                                                                           <  1:1323003/100‑1 (MQ=255)

                         gACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGAt                                                                          <  2:2262509/100‑1 (MQ=255)

                          aCCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGTCAGTGATCCAGTTTATCCAGATCGTTGAGCAACGCGTCAGCGTAAGCAGTGA‑TTTTGATa                                                                         <  1:2914875/100‑1 (MQ=255)

                            cGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATaaa                                                                       <  2:75837/100‑1 (MQ=255)

                             gATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAAc                                                                      >  1:2584188/1‑100 (MQ=255)

                             gATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAAc                                                                      >  2:1345494/1‑100 (MQ=255)

                              aTCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAAcc                                                                     <  2:85428/100‑1 (MQ=255)

                              aTCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAAcc                                                                     >  2:2953996/1‑100 (MQ=255)

                                  aGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACt                                                                  >  1:2205712/1‑99 (MQ=255)

                                    ttACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGAGCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCg                                                               <  1:3270589/100‑1 (MQ=255)

                                     tACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCgg                                                              <  2:1900868/100‑1 (MQ=255)

                                          tGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGAtct                                                         >  2:1976642/1‑100 (MQ=255)

                                           gCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTCTCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGAtctc                                                        >  1:1805650/1‑100 (MQ=255)

                                           gCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGCTAAAAAACTGCGGGCtctc                                                        >  2:2318934/1‑100 (MQ=255)

                                           gCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGAtctc                                                        <  1:2455750/100‑1 (MQ=255)

                                            cATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGAtctc                                                        >  1:289288/1‑99 (MQ=255)

                                             gtGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCtt                                                      >  2:1242639/2‑100 (MQ=255)

                                              tGGTTTTCACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCttt                                                     <  1:1814732/100‑1 (MQ=255)

                                              tGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCttt                                                     <  1:82972/100‑1 (MQ=255)

                                              tGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCtt                                                      >  1:434647/1‑99 (MQ=255)

                                                gTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTAc                                                   >  2:2698745/1‑100 (MQ=255)

                                                gTTTTAACGGTGTCTGCCCAATGATACAGTTTATCCAAATAGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTTATAAACCATTGCGGGATCTCTTTAc                                                   >  1:720659/1‑100 (MQ=255)

                                                 ttttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAAATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACg                                                  <  2:2384281/100‑1 (MQ=255)

                                                  tttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGt                                                 >  2:360394/1‑100 (MQ=255)

                                                   ttAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGtt                                                <  1:162564/100‑1 (MQ=255)

                                                    tAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTc                                               <  1:3053777/100‑1 (MQ=255)

                                                    tAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTc                                               >  1:2072777/1‑100 (MQ=255)

                                                     aaCGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCa                                              >  2:1501245/1‑100 (MQ=255)

                                                       cGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAAc                                            <  1:1111928/100‑1 (MQ=255)

                                                       cGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAAc                                            >  2:428443/1‑100 (MQ=255)

                                                       cGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAAc                                            <  1:750776/100‑1 (MQ=255)

                                                       cGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAAc                                            >  1:434743/1‑100 (MQ=255)

                                                          tgtCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACttt                                         <  2:483793/100‑1 (MQ=255)

                                                          tgtCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACttt                                         <  2:2731887/100‑1 (MQ=255)

                                                           gtCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACAACTGCGGGATCTCTTTACGTTCAACTTTg                                        >  2:2927564/1‑100 (MQ=255)

                                                            tCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTgg                                       <  2:2072790/100‑1 (MQ=255)

                                                              tGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTa                                     <  2:434745/100‑1 (MQ=255)

                                                                gCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATc                                   >  2:1559316/1‑100 (MQ=255)

                                                                 ccAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCg                                  <  2:2944315/100‑1 (MQ=255)

                                                                  cAGTGATCCAGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGc                                 >  2:2256512/1‑100 (MQ=255)

                                                                   aGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCa                                >  2:2335264/1‑100 (MQ=255)

                                                                   aGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCa                                <  2:728765/100‑1 (MQ=255)

                                                                         ccAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCca                          <  2:2315431/100‑1 (MQ=255)

                                                                         ccAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCca                          >  1:1077576/1‑100 (MQ=255)

                                                                           aGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagc                        <  2:1830536/100‑1 (MQ=255)

                                                                           aGTTTATCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagc                        <  2:805106/100‑1 (MQ=255)

                                                                             tttATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcag                      <  2:1200557/100‑1 (MQ=255)

                                                                                aTCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcagcag                   <  1:2124277/100‑1 (MQ=255)

                                                                                aTCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcagcag                   >  2:1267207/1‑100 (MQ=255)

                                                                                aTCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCcagcagcag                   >  2:3148867/1‑100 (MQ=255)

                                                                                 tCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCagcagcagc                  >  2:1012652/1‑100 (MQ=255)

                                                                                 tCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCagcagcagc                  >  1:34880/1‑100 (MQ=255)

                                                                                 tCCAGATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCagcagcagc                  >  2:628797/1‑100 (MQ=255)

                                                                                   cAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCg                >  2:178362/1‑100 (MQ=255)

                                                                                   cAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCg                >  1:1536361/1‑100 (MQ=255)

                                                                                   cAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCg                <  2:3001396/100‑1 (MQ=255)

                                                                                     gATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGtn              <  1:845529/100‑2 (MQ=255)

                                                                                     gATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTc              <  1:1957874/100‑1 (MQ=255)

                                                                                     gATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTc              <  2:1184189/100‑1 (MQ=255)

                                                                                     gATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTc              <  2:1681908/100‑1 (MQ=255)

                                                                                     gATCGTTGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTc              >  1:2976826/1‑100 (MQ=255)

                                                                                          ttGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATaa         >  2:1583915/1‑100 (MQ=255)

                                                                                          ttGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATaa         >  2:508039/1‑100 (MQ=255)

                                                                                          ttGAGCAACTCGTCAGCGTAAGCAGTGATTTTTTTTTTTTTTCTGCGGGATCTCTTTACGTTCAACTTTGGTCTCGCAGCGCCCGCAGCAGCCGTCGAt           >  1:2240785/1‑99 (MQ=255)

                                                                                           tGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAAc        >  2:1568482/1‑100 (MQ=255)

                                                                                           tGAGCAACTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAAc        <  1:2998719/100‑1 (MQ=255)

                                                                                             agcagcTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACtt      <  2:1943834/100‑1 (MQ=255)

                                                                                              tcagcTCGTCAGCGTAGGCAGTGA‑TTTGGATAAACCACGGCGGGATCTCGGTACGTTCAACTTTGGTATCGCAGCGCCGGCAGCAGCCGTCGATAACtt      <  1:1637639/98‑1 (MQ=255)

                                                                                              gcagcTCGTCAGCGTAAGCAGTGA‑TCTGGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTg     <  1:218926/100‑1 (MQ=255)

                                                                                               cagcTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACTTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTgt    <  2:799212/100‑1 (MQ=255)

                                                                                                 gcTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTgttc  >  2:1346629/1‑100 (MQ=255)

                                                                                                 gcTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTgttc  <  1:1228259/100‑1 (MQ=255)

                                                                                                 gcTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTgttc  >  2:899575/1‑100 (MQ=255)

AAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGA‑TTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTC  >  NC_000913/674083‑674279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`