Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 53,012 A→G 61.4% G135G (GGT→GGC)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091353,0120AG61.4% 121.8 / 141.4 144G135G (GGT→GGC) pdxA4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (26/29);  new base G (37/50);  total (63/81)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

CGGTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCTC  >  NC_000913/52913‑53109
                                                                                                   |                                                                                                 
cGGTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCACCATCATCACCACCCTTCTCGCCTGCGAACGCTCTTCGAAAAACTCGATATGa                                                                                                   >  1:2747003/1‑100 (MQ=255)
   tAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCg                                                                                                <  1:2115796/100‑1 (MQ=255)
    aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCgg                                                                                               <  2:1061617/100‑1 (MQ=255)
    aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCgg                                                                                               <  1:2277573/100‑1 (MQ=255)
     aaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGt                                                                                              <  2:738316/100‑1 (MQ=255)
      aTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGCACGCTCTTCGAAAAACTCGGTATGGCCGGTa                                                                                             >  1:1181097/1‑100 (MQ=255)
      aTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTGCGAAAAACTCGGTATGGCCGGTa                                                                                             >  1:1060715/1‑100 (MQ=255)
      aTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTCTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTa                                                                                             >  2:335801/1‑100 (MQ=255)
       tGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTaa                                                                                            >  2:1015167/1‑100 (MQ=255)
         cgtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTaaaa                                                                                          <  2:2511631/100‑1 (MQ=255)
          gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                         >  2:2449593/1‑100 (MQ=255)
          gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                         <  2:2365159/100‑1 (MQ=255)
          gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAg                                                                                         <  2:2552466/100‑1 (MQ=255)
           tcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAgg                                                                                        <  1:1555793/100‑1 (MQ=255)
           tcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATG‑GCGGTAAAAGGa                                                                                       <  1:751249/100‑1 (MQ=255)
              ttGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAAt                                                                                     <  1:2533260/100‑1 (MQ=255)
               tGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATg                                                                                    <  1:218594/100‑1 (MQ=255)
                gCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGc                                                                                   >  1:2418521/1‑100 (MQ=255)
                 ccAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTTTTTTTTTTTCGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGc                                                                                   >  1:656153/1‑99 (MQ=255)
                 ccAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGcc                                                                                  <  2:236663/100‑1 (MQ=255)
                  cAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCa                                                                                 >  2:494959/1‑100 (MQ=255)
                   aGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAg                                                                                <  2:1570319/100‑1 (MQ=255)
                   aGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTAAGACCGGTAAAAGGAATGCCAg                                                                                <  1:1975262/100‑1 (MQ=255)
                     cgcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcg                                                                              <  2:670649/100‑1 (MQ=255)
                     cgcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcg                                                                              <  2:111481/100‑1 (MQ=255)
                       ccACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtc                                                                            <  2:1425861/100‑1 (MQ=255)
                         aCGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtcgt                                                                          >  2:2879486/1‑100 (MQ=255)
                          cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCTAACGCCCTTCGAAAAACTCGGTATGGCCGGCAAAAGGAATGCCAGcgtc                                                                            >  1:58461/1‑97 (MQ=255)
                            cgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTtaa                                                                       <  2:2273991/100‑1 (MQ=255)
                               aGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTtaataa                                                                    <  2:2919505/100‑1 (MQ=255)
                                   cttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGcc                                                                <  1:3380811/100‑1 (MQ=255)
                                   cttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCGCTTCGAAAACCTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGc                                                                 <  1:1239846/99‑1 (MQ=255)
                                    ttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCt                                                               >  1:366203/1‑100 (MQ=255)
                                      cGGTCCCCAGCATCCTCACCACCTTTTTCGCCTGCGAACGCTCTTAGCAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTCCTAACGCCtt                                                              <  2:1844894/99‑1 (MQ=255)
                                       ggacGCCAGCATCATCACCACCTTTTTTGATTGCAAACGCTCATCGCAAAAATCGGTATCAAAGGTAAATGGAACTCGAGCGTCGTtaatatc                                                                   >  1:3274268/4‑91 (MQ=255)
                                        gTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTAt                                                           >  1:825551/1‑100 (MQ=255)
                                         tCGCCAGCATCATCACCCCCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATg                                                          <  2:842847/100‑1 (MQ=255)
                                         tCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATACCGCCTTTATg                                                          <  1:1292455/100‑1 (MQ=255)
                                         tCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATg                                                          <  2:793542/100‑1 (MQ=255)
                                         tCGCCAGCATCATCACCACCTTTTTCGCCCGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                            <  1:2985123/98‑1 (MQ=255)
                                          cGCCAGCCTCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                             <  1:3171813/96‑1 (MQ=255)
                                          cGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGc                                                         <  2:3169161/100‑1 (MQ=255)
                                          cGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGc                                                         <  2:2855542/100‑1 (MQ=255)
                                          cGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGc                                                         >  2:1036235/1‑100 (MQ=255)
                                            ccAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAc                                                       <  2:1867028/100‑1 (MQ=255)
                                            ccAGCATCATCACCACCTTTTTAGCCTGCGAACGCTCTTCAAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCCTTAATAACGCCTTTATGCAc                                                       >  1:2749102/1‑100 (MQ=255)
                                             cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                      <  1:862372/100‑1 (MQ=255)
                                             cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                      <  2:961936/100‑1 (MQ=255)
                                              aGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCg                                                     >  1:1422775/1‑100 (MQ=255)
                                               gCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCgg                                                    >  2:964905/1‑100 (MQ=255)
                                                   catcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCGGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGAACCGGAcca                                                >  1:2596411/1‑99 (MQ=255)
                                                   catcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGCCCt                                                >  1:1252325/1‑100 (MQ=255)
                                                   catcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt                                                >  2:855486/1‑100 (MQ=255)
                                                     tcaCCACCTTTTTCGCCTGCGAACGCTCTTCTAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                              <  1:2790617/100‑1 (MQ=255)
                                                     tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                              <  2:3380917/100‑1 (MQ=255)
                                                       accaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGa                                            <  2:1555135/100‑1 (MQ=255)
                                                       accaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGa                                            <  1:3008170/100‑1 (MQ=255)
                                                        ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                           <  2:823401/100‑1 (MQ=255)
                                                        ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                           <  1:2526172/100‑1 (MQ=255)
                                                        ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                           >  1:1561291/1‑100 (MQ=255)
                                                        ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                           <  1:1727422/100‑1 (MQ=255)
                                                         caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                          >  2:2211970/1‑100 (MQ=255)
                                                         caccGTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                          <  2:1587536/100‑1 (MQ=255)
                                                             ttttttGCCTGCGAACGCTCTTCTAAAATCTCGGTATGAGCGGTAAAAGGAATGCCATCGTCGTTAATATCGCGTTTATGCACCGGACCTGTGATc                                          <  1:3196828/96‑1 (MQ=255)
                                                             tttttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcg                                      >  2:1226342/1‑100 (MQ=255)
                                                              ttttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcgc                                     <  2:776683/100‑1 (MQ=255)
                                                              ttttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcgc                                     <  2:2444971/100‑1 (MQ=255)
                                                               tttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcgc                                     <  1:2953650/99‑1 (MQ=255)
                                                                 tCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGc                                  >  1:3252503/1‑100 (MQ=255)
                                                                  cGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCa                                 >  1:2725795/1‑100 (MQ=255)
                                                                     cTGCGAACGATCTTCGAAAAACGCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATCACGCCTTTATGCACCGGACCTGTGATCAGCGCgg                                   <  1:1465325/95‑1 (MQ=255)
                                                                       gCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTc                            <  2:521881/100‑1 (MQ=255)
                                                                         gAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGc                          >  1:2681079/1‑100 (MQ=255)
                                                                            cGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGt                       >  2:1653107/1‑100 (MQ=255)
                                                                            cGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGt                       >  1:584897/1‑100 (MQ=255)
                                                                             gCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGtt                      <  2:665749/100‑1 (MQ=255)
                                                                              ctctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                     <  2:1872629/100‑1 (MQ=255)
                                                                              ctctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                     <  2:496175/100‑1 (MQ=255)
                                                                               tctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCa                    <  1:173768/100‑1 (MQ=255)
                                                                               tctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCa                    <  2:1215053/100‑1 (MQ=255)
                                                                               tctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCa                    >  2:2090060/1‑100 (MQ=255)
                                                                                ctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                   >  1:2232351/1‑100 (MQ=255)
                                                                                ctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                   >  2:862482/1‑100 (MQ=255)
                                                                                ctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                   >  2:2207577/1‑100 (MQ=255)
                                                                                 ttCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGa                  <  1:1402670/100‑1 (MQ=255)
                                                                                  tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc                 <  2:2194726/100‑1 (MQ=255)
                                                                                  tCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc                 <  2:10324/100‑1 (MQ=255)
                                                                                   cGAAAAACTCGGTATGACCTGAAAAAGGAATGCCAACGTCTGTAATAACTCCTTTATGCACAGGACCTGTGATCAGCGCGCCAAATTCGCCGTTcacaca                >  1:2877028/1‑100 (MQ=255)
                                                                                     aaaaaCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAc              <  1:825342/100‑1 (MQ=255)
                                                                                      aaaaCTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAcc             <  1:2795630/100‑1 (MQ=255)
                                                                                      aaaaCTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAcc             <  1:448759/100‑1 (MQ=255)
                                                                                         aCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATc          >  1:1242570/1‑100 (MQ=255)
                                                                                         aCTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATc          >  1:1077273/1‑100 (MQ=255)
                                                                                          cTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCg         <  2:2765954/100‑1 (MQ=255)
                                                                                          cTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCg         <  1:3004396/100‑1 (MQ=255)
                                                                                           tCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGc        >  2:392828/1‑100 (MQ=255)
                                                                                           tCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGc        >  1:841129/1‑100 (MQ=255)
                                                                                              gTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACg     <  2:1007265/100‑1 (MQ=255)
                                                                                               tATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGc    >  2:1929581/1‑100 (MQ=255)
                                                                                               tATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGc    <  1:967064/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
CGGTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCTC  >  NC_000913/52913‑53109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑