Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 894,155 T→G 100% D124E (GAT→GAG potF → putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913894,1550TG98.2% 375.8 / ‑3.1 112D124E (GAT→GAGpotFputrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (53/55);  total (56/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 112 total aligned reads displayed.

GTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCC  >  NC_000913/894059‑894251
                                                                                                |                                                                                                
gTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGccc                                                                                               <  1:793402/100‑1 (MQ=255)
  tGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGGGCCCGa                                                                                             >  2:174079/1‑100 (MQ=255)
  tGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGa                                                                                             <  2:3346552/100‑1 (MQ=255)
   gACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAATCTGGTCGCCAAACACGAGCCCGAc                                                                                            >  2:677099/1‑100 (MQ=255)
   gACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGAc                                                                                            <  2:2516265/100‑1 (MQ=255)
   gACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGAc                                                                                            >  1:2821594/1‑100 (MQ=255)
   gACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGAc                                                                                            >  1:2903214/1‑100 (MQ=255)
    aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACa                                                                                           >  2:2305365/1‑100 (MQ=255)
    aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACa                                                                                           <  2:1527820/100‑1 (MQ=255)
     cTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACaa                                                                                          <  2:315970/100‑1 (MQ=255)
      tGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAAt                                                                                         <  2:2352277/100‑1 (MQ=255)
        cGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTAGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATaa                                                                                       >  1:921173/1‑100 (MQ=255)
          ggAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAt                                                                                     >  2:2573449/1‑100 (MQ=255)
           gAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAtt                                                                                    <  2:1238318/100‑1 (MQ=255)
            aGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAttt                                                                                   <  2:816651/100‑1 (MQ=255)
             gTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTg                                                                                  <  1:822427/100‑1 (MQ=255)
              ttttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGc                                                                                 <  2:167694/100‑1 (MQ=255)
               tttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGc                                                                                 >  1:2034102/1‑99 (MQ=255)
               tttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCt                                                                                >  2:324941/1‑100 (MQ=255)
               tttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCt                                                                                >  2:1000926/1‑100 (MQ=255)
               tttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCt                                                                                >  1:511357/1‑100 (MQ=255)
                 tCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTAt                                                                              >  1:1418466/1‑100 (MQ=255)
                   cAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGc                                                                            >  2:187990/1‑100 (MQ=255)
                   cAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGc                                                                            >  2:1491161/1‑100 (MQ=255)
                     gCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGccc                                                                          >  1:3309239/1‑100 (MQ=255)
                     gCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGccc                                                                          >  1:1766744/1‑100 (MQ=255)
                     gCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGccc                                                                          <  2:1587225/100‑1 (MQ=255)
                     gCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTAAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGccc                                                                          <  1:2561890/100‑1 (MQ=255)
                       cGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATCAATTTGCTATGCCCta                                                                        >  1:501586/1‑100 (MQ=255)
                       cGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCta                                                                        >  1:2174468/1‑100 (MQ=255)
                        gCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCtat                                                                       >  1:2867605/1‑100 (MQ=255)
                        gCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCtat                                                                       <  2:2411476/100‑1 (MQ=255)
                        gCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACAAGCCCGACAATAAATTTGTTATGCCCtat                                                                       >  2:620383/1‑100 (MQ=255)
                           ggccAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCATACACGCGCACGACAATAAATTTGCTATGCCCTATAtg                                                                    >  1:2287136/4‑100 (MQ=255)
                            gACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgt                                                                   >  1:282605/1‑100 (MQ=255)
                             aCAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgtg                                                                  <  1:2848063/100‑1 (MQ=255)
                              cAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACAAGCCCGACAATAAATTTGCTATGCCCTATATGTgg                                                                 >  2:2357070/1‑100 (MQ=255)
                               aaaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTggg                                                                <  1:635324/100‑1 (MQ=255)
                               aaaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTggg                                                                <  2:1245714/100‑1 (MQ=255)
                               aaaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTggg                                                                <  2:1418472/100‑1 (MQ=255)
                                aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               <  1:1558474/100‑1 (MQ=255)
                                aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               >  1:2277348/1‑100 (MQ=255)
                                aaaGCAAATTGCCGGAGGGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGCCAACAAATTTGCTATGCCCTATATGTggcc                                                               >  1:1002975/1‑98 (MQ=255)
                                aaaGCAAATTGCCGGACTGGAAGAATCTCGACCAGGAACTGCTGAAGCTGGGCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               >  1:2830978/1‑100 (MQ=255)
                                 aaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGCAGCTGGTCGCCAAACACGACCCCGACAATAAATTTGCTATGCCCTATATGTGGGcg                                                              >  1:1303739/1‑100 (MQ=255)
                                 aaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGGGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcg                                                              <  1:3290240/100‑1 (MQ=255)
                                   gCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcgac                                                            <  2:3116332/100‑1 (MQ=255)
                                       aTTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGAcc                                                        <  2:860729/100‑1 (MQ=255)
                                         tGCCGGAGTGGAAGAATCTCGCCCCGGAACGGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTAGATGTGGGCGACGACCgg                                                      <  2:1805881/100‑1 (MQ=255)
                                         tGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCgg                                                      >  1:3110256/1‑100 (MQ=255)
                                         tGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCgg                                                      <  1:1750153/100‑1 (MQ=255)
                                         tGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCgg                                                      <  2:2848218/100‑1 (MQ=255)
                                          ggcGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCggg                                                     >  2:2293735/3‑100 (MQ=255)
                                           ccGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGa                                                    >  2:442142/1‑100 (MQ=255)
                                              gAGTGGAAGAATCTCGTCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTg                                                 <  1:1985138/100‑1 (MQ=255)
                                                gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGCGCCCGACAATCAATTTGCCATGCCCTATCTGTGCGCGACGACCGCGATTGGc                                               <  1:2830021/100‑1 (MQ=255)
                                                gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGc                                               <  2:964761/100‑1 (MQ=255)
                                                 tGGAAGAATCTCGATCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                              <  1:2305349/100‑1 (MQ=255)
                                                 tGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                              <  1:3078146/100‑1 (MQ=255)
                                                  ggAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                             >  1:913226/1‑100 (MQ=255)
                                                   gaagaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGTtat                                            >  1:350682/1‑100 (MQ=255)
                                                    aagaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCtata                                           >  2:1545949/1‑100 (MQ=255)
                                                      gaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAc                                         <  1:2411385/100‑1 (MQ=255)
                                                           tcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGa                                    <  1:563585/100‑1 (MQ=255)
                                                           tcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGCCTATAACGTTGa                                    >  1:1784546/1‑100 (MQ=255)
                                                            cGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGAt                                   <  1:1392981/100‑1 (MQ=255)
                                                            cGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGAt                                   <  1:436638/100‑1 (MQ=255)
                                                             gACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATa                                  <  2:1347432/100‑1 (MQ=255)
                                                             gACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATa                                  >  2:1466933/1‑100 (MQ=255)
                                                              aCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATaa                                 <  2:2745854/100‑1 (MQ=255)
                                                               cccGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATaaa                                <  2:1943005/100‑1 (MQ=255)
                                                                ccGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAg                               <  2:362400/100‑1 (MQ=255)
                                                                 cGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGt                              <  2:1151332/100‑1 (MQ=255)
                                                                 cGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGt                              <  2:2867628/100‑1 (MQ=255)
                                                                 cGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGCCTATAACGTTGATAAAGt                              <  2:1784557/100‑1 (MQ=255)
                                                                 cGGAACTGCTGAAGCTGGTCGCCAAACACAAGCCCGACAAAAATTTTGCTATGTCCTATATGTGGGCGACGACCGGGATTCGCTATAAAGTTGATAAAg                               <  1:3154127/99‑1 (MQ=255)
                                                                    aaCTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAGTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGt                              <  2:2007909/97‑1 (MQ=255)
                                                                     aCTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTaaa                          >  2:1465789/1‑100 (MQ=255)
                                                                     aCTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTACATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTaaa                          >  1:3167038/1‑100 (MQ=255)
                                                                       tgctgAACCTGGTCGCCAAACACGAACCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGc                        >  1:281575/1‑100 (MQ=255)
                                                                        gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  1:1054775/1‑100 (MQ=255)
                                                                        gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       <  1:1334241/100‑1 (MQ=255)
                                                                         ctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCgg                      >  1:1734404/1‑100 (MQ=255)
                                                                          tgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGt                     >  2:2805424/1‑100 (MQ=255)
                                                                           gAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTaaa                          >  2:2852352/1‑94 (MQ=255)
                                                                            aaGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGc                   <  1:2916879/100‑1 (MQ=255)
                                                                            aaGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGc                   >  2:1336385/1‑100 (MQ=255)
                                                                               cTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTgg                >  1:178554/1‑100 (MQ=255)
                                                                                tGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTggg               >  2:2809550/1‑100 (MQ=255)
                                                                                 ggTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc              <  1:949722/100‑1 (MQ=255)
                                                                                 ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc              <  1:558589/100‑1 (MQ=255)
                                                                                   tCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGa            >  1:9950/1‑100 (MQ=255)
                                                                                   tCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGa            >  1:1348015/1‑100 (MQ=255)
                                                                                      ccAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaaaa         >  1:1736042/1‑100 (MQ=255)
                                                                                      ccAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaaaa         >  1:1240440/1‑100 (MQ=255)
                                                                                       cAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAc        >  2:2402578/1‑100 (MQ=255)
                                                                                           cacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACgcgc    >  2:1216711/1‑100 (MQ=255)
                                                                                            acGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACgcgc    <  1:1350343/99‑1 (MQ=255)
                                                                                             cGAGCCCGACAATCAATTTGCTATGCCCTATATGTGGGCGACGACCGGGACTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGcc   <  1:153435/99‑1 (MQ=255)
                                                                                             cGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGccc  >  1:3127186/1‑100 (MQ=255)
                                                                                                |                                                                                                
GTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCC  >  NC_000913/894059‑894251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: