Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 975,083 T→A 63.4% D255E (GAT→GAA mukF → chromosome condensin MukBEF, kleisin‑like subunit, binds calcium

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913975,0830TA63.4% 142.2 / 148.6 144D255E (GAT→GAAmukFchromosome condensin MukBEF, kleisin‑like subunit, binds calcium
Reads supporting (aligned to +/- strand):  ref base T (18/34);  new base A (35/55);  total (55/89)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

GAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCG  >  NC_000913/974985‑975180
                                                                                                  |                                                                                                 
gAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATc                                                                                                  <  2:3158765/100‑1 (MQ=255)
 aaGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCt                                                                                                 <  1:2166525/100‑1 (MQ=255)
   gCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGc                                                                                               <  1:3223904/100‑1 (MQ=255)
    cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCGTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCa                                                                                              >  1:2372113/1‑100 (MQ=255)
    cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCa                                                                                              <  2:1109527/100‑1 (MQ=255)
          gCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCaa                                                                                        >  2:2402282/1‑100 (MQ=255)
          gCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCaa                                                                                        <  1:209920/100‑1 (MQ=255)
          gCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCaa                                                                                        >  2:237011/1‑100 (MQ=255)
          gCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCaa                                                                                        >  2:1807598/1‑100 (MQ=255)
            gACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAAc                                                                                      >  2:3279351/1‑100 (MQ=255)
            gACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAAc                                                                                      >  1:481526/1‑100 (MQ=255)
             aCAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTTGATCTGCAGAGCAAACt                                                                                     >  1:838259/1‑100 (MQ=255)
             aCAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCGGAGCAAACt                                                                                     <  1:2573736/100‑1 (MQ=255)
              cAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTc                                                                                    >  2:71255/1‑100 (MQ=255)
                 aTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGAt                                                                                 <  1:447526/100‑1 (MQ=255)
                  ttGCAGGCTAATCTGTTGCGCATTCATGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATc                                                                                <  1:2259754/100‑1 (MQ=255)
                   tgtaGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCg                                                                               <  2:553850/97‑1 (MQ=255)
                   tGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCg                                                                               <  2:3028881/100‑1 (MQ=255)
                   tGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCg                                                                               <  1:2294025/100‑1 (MQ=255)
                    gCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGt                                                                              >  2:799509/1‑100 (MQ=255)
                     cAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGta                                                                             >  2:944337/1‑100 (MQ=255)
                      aGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGtat                                                                            <  1:1694082/100‑1 (MQ=255)
                       ggCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatt                                                                           >  1:1925715/1‑100 (MQ=255)
                       ggCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGt                                                                              >  1:1070872/1‑97 (MQ=255)
                        gCTAATCGGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatt                                                                           <  1:1980787/99‑1 (MQ=255)
                         cTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGtatta                                                                          >  2:1589575/1‑99 (MQ=255)
                          tAATCTGTTGCGCATTCAGGATGCGACGATGGCCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATc                                                                        <  1:1262082/100‑1 (MQ=255)
                           aaTCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCa                                                                       <  1:588387/100‑1 (MQ=255)
                           aaTCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTACTATCa                                                                       >  1:3136468/1‑100 (MQ=255)
                            aTCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAg                                                                      <  2:894895/100‑1 (MQ=255)
                            aTCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAg                                                                      <  1:1604198/100‑1 (MQ=255)
                            aTCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAg                                                                      >  1:702112/1‑100 (MQ=255)
                             tCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGt                                                                     <  2:2951499/100‑1 (MQ=255)
                              cTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGtt                                                                    <  2:1805153/100‑1 (MQ=255)
                               tGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTg                                                                   >  2:766084/1‑100 (MQ=255)
                                gTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTgg                                                                  >  2:408080/1‑100 (MQ=255)
                                gTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAGCTGCACAGCAAACTCGATCGTATTATCAGTTg                                                                   >  1:1298464/1‑99 (MQ=255)
                                gTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgg                                                                  >  1:4355/1‑100 (MQ=255)
                                 ttGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTggg                                                                 <  2:3071624/100‑1 (MQ=255)
                                 ttGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTggg                                                                 <  1:887719/100‑1 (MQ=255)
                                  tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                                <  1:943112/100‑1 (MQ=255)
                                  tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                                <  1:791170/100‑1 (MQ=255)
                                   gagcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTAGATCGTATTATCAGTTGGGGc                                                               <  1:1342226/98‑1 (MQ=255)
                                    cgcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGcc                                                              >  2:50529/1‑100 (MQ=255)
                                    cgcATTCAGGATGCGACGATGACCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCa                                                             >  2:50531/1‑100 (MQ=255)
                                      cATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAg                                                            <  2:1415071/100‑1 (MQ=255)
                                      cATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAg                                                            >  1:211422/1‑100 (MQ=255)
                                      aaTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTGCGAACTGCAGAGCAAACTCGAGCGCAGTATCAg                                                                      >  1:3189990/2‑90 (MQ=255)
                                       aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc                                                           <  1:1024651/100‑1 (MQ=255)
                                       aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc                                                           <  1:1939685/100‑1 (MQ=255)
                                       aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc                                                           <  1:791876/100‑1 (MQ=255)
                                          cAGGATGCGACGATGACCCACGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAAt                                                        >  1:2074828/1‑100 (MQ=255)
                                           aGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATc                                                       <  2:787124/100‑1 (MQ=255)
                                           aGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCCAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATc                                                       <  1:1875523/100‑1 (MQ=255)
                                            ggATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGCTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATcc                                                      >  2:201641/1‑100 (MQ=255)
                                              aTGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCAt                                                    <  1:1231849/100‑1 (MQ=255)
                                              aTGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCAt                                                    <  2:481528/100‑1 (MQ=255)
                                               tGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATc                                                   >  1:1095770/1‑100 (MQ=255)
                                                gCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATc                                                   <  1:562444/99‑1 (MQ=255)
                                                gCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCg                                                  <  2:1925728/100‑1 (MQ=255)
                                                 cgacgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGa                                                 >  2:1728736/1‑100 (MQ=255)
                                                 cgacgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGa                                                 >  1:3307725/1‑100 (MQ=255)
                                                       tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCacaagtcgatg                                           <  2:211422/100‑11 (MQ=255)
                                                       tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgtg                                           <  2:1717178/100‑1 (MQ=255)
                                                        gACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGGTCGTATTAGCAGTTGGGGCCAGCAATCCATCGACTtgtg                                           <  1:1392362/99‑1 (MQ=255)
                                                        gACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTgg                                          >  1:3064119/1‑100 (MQ=255)
                                                         aCCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGa                                         <  2:2191793/100‑1 (MQ=255)
                                                         aCCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGa                                         >  2:1137623/1‑100 (MQ=255)
                                                             aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                     <  1:2563048/100‑1 (MQ=255)
                                                             aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                     <  2:1590462/100‑1 (MQ=255)
                                                             aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                     <  2:2734424/100‑1 (MQ=255)
                                                             aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTg                                      >  1:2836927/1‑99 (MQ=255)
                                                             aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                     <  1:1500762/100‑1 (MQ=255)
                                                             aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                     <  2:126324/100‑1 (MQ=255)
                                                              tGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGc                                    <  2:1206607/100‑1 (MQ=255)
                                                              tGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGc                                    <  1:529251/100‑1 (MQ=255)
                                                               gACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCt                                   >  2:2556923/1‑100 (MQ=255)
                                                               gACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCt                                   <  1:1157997/100‑1 (MQ=255)
                                                                aCGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTa                                  <  1:3307463/100‑1 (MQ=255)
                                                                aCGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTa                                  <  1:2152992/100‑1 (MQ=255)
                                                                 cGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTAc                                 <  2:1284418/100‑1 (MQ=255)
                                                                        cATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGcc                          >  2:2669526/1‑100 (MQ=255)
                                                                         aTTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCa                         <  2:2425779/100‑1 (MQ=255)
                                                                           ttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACg                       >  1:2654723/1‑100 (MQ=255)
                                                                             cgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTa                     <  2:2135453/100‑1 (MQ=255)
                                                                             cgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTa                     <  2:2014448/100‑1 (MQ=255)
                                                                              gtcgATGGTCTGGTCTTCGATCTGCATAGCATTCTCGATCGTGTTATCAGTTGTGGCCAGCAATCTATCGACTTGTTTATTGGCTACGACCGCCACGTac                    <  1:2338063/100‑1 (MQ=255)
                                                                              gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTac                    >  2:2114427/1‑100 (MQ=255)
                                                                              gtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTac                    >  1:2446295/1‑100 (MQ=255)
                                                                                cgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTacac                  <  1:1253344/100‑1 (MQ=255)
                                                                                cgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTacac                  >  2:3288831/1‑100 (MQ=255)
                                                                                cgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTacac                  <  2:2925290/100‑1 (MQ=255)
                                                                                 gATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCATTTGGGATCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTaca                   >  1:2061349/1‑98 (MQ=255)
                                                                                   tCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACaaa               <  1:2331333/100‑1 (MQ=255)
                                                                                        tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAt          <  1:528340/100‑1 (MQ=255)
                                                                                         ggTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAtt         <  1:567943/100‑1 (MQ=255)
                                                                                           tgtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCg       <  2:1460172/100‑1 (MQ=255)
                                                                                            gtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGt      >  1:19860/1‑100 (MQ=255)
                                                                                              tCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTAc    >  1:1465326/1‑100 (MQ=255)
                                                                                               cGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATGGGCTACGACCGCCACGTACACAAATTTATTCGTAcc   >  2:2378209/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
GAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCG  >  NC_000913/974985‑975180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: