Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 129,360 G→T 60.8% intergenic (+24/+47) lpd → / ← yacH dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913129,3600GT60.8% 96.8 / 195.6 158intergenic (+24/+47)lpd/yacHdihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein
Reads supporting (aligned to +/- strand):  ref base G (31/31);  new base T (51/45);  total (82/76)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 161 total aligned reads displayed.

GTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTG  >  NC_000913/129265‑129459
                                                                                               |                                                                                                   
gtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAt                                                                                                 <  2:1506928/100‑1 (MQ=255)
 tgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGATGTTCCGGCaaac                                                                                                <  2:500994/100‑3 (MQ=255)
   ggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTaa                                                                                              >  1:689359/1‑100 (MQ=255)
    gCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaa                                                                                             >  2:2382260/1‑100 (MQ=255)
    gCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaa                                                                                             >  2:1008937/1‑100 (MQ=255)
    gCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaa                                                                                             >  1:2780953/1‑100 (MQ=255)
    gCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTaaa                                                                                             <  2:2325367/100‑1 (MQ=255)
       tGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAGAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaaaa                                                                                          >  2:2020076/1‑100 (MQ=255)
        ggcggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAg                                                                                         >  1:1385833/1‑100 (MQ=255)
          cggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCg                                                                                       <  1:221867/100‑1 (MQ=255)
          cggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCg                                                                                       <  2:855331/100‑1 (MQ=255)
           ggcAGAAGTGTTCGAATGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCgg                                                                                      <  1:1975160/100‑1 (MQ=255)
            gcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGCTTGCCGGAACATCCGTCAATTAAAAAAGCGGc                                                                                     <  1:1127467/100‑1 (MQ=255)
             cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCt                                                                                    >  1:1313899/1‑100 (MQ=255)
             cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCt                                                                                    >  2:879617/1‑100 (MQ=255)
               gAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTaa                                                                                  >  2:2879600/1‑100 (MQ=255)
                 aGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCTTTTTCTTGAACATCCGTCAATTAAAAAAGCGGCTAAcc                                                                                >  1:2462743/1‑100 (MQ=255)
                   tgtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAc                                                                              <  2:2920124/100‑1 (MQ=255)
                    gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTTCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcg                                                                             >  1:1769550/1‑100 (MQ=255)
                    gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcg                                                                             >  1:2686795/1‑100 (MQ=255)
                      tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGcgtggttagcc                                                                           >  2:1422631/1‑90 (MQ=255)
                      tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgcc                                                                           <  2:343599/100‑1 (MQ=255)
                       cGAAGGTAGCATTACCGACCTGCCGAACCCGAGAGCGAACAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgccg                                                                          <  1:2382243/100‑1 (MQ=255)
                       cGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATGCGTCAATTAAAAAAGCGGCTAACCAcgccg                                                                          >  2:1168849/1‑100 (MQ=255)
                        gaagaaaGCATTACCGACCTGCCAAACCCGAAAGCGAAGAAGAAGTAATTTATCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgc                                                                            <  1:1782892/91‑1 (MQ=255)
                        gAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgccgc                                                                         >  2:685223/1‑100 (MQ=255)
                        gAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcgccgc                                                                         <  1:380101/100‑1 (MQ=255)
                        gAAGGTAACATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcgccgc                                                                         >  2:1771739/1‑100 (MQ=255)
                          aGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCtt                                                                       >  1:962363/1‑100 (MQ=255)
                           ggTAGCATTACCGACCTGTCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCt                                                                        >  1:3081836/1‑98 (MQ=255)
                           ggTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTTTTTTCACATCCGTCAATTAAAAAAGCGTCTAACCACGACGCttt                                                                      >  1:2861774/1‑100 (MQ=255)
                            gTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtttt                                                                     <  2:857628/100‑1 (MQ=255)
                            gTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCtttt                                                                     >  1:1566438/1‑100 (MQ=255)
                             tAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCttttt                                                                    <  1:2047536/100‑1 (MQ=255)
                               gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCttttttt                                                                  <  1:1723700/100‑1 (MQ=255)
                               gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCttttttt                                                                  >  1:1532271/1‑100 (MQ=255)
                                cATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATATTGCGTTTGCCGGAACATCCGTCTATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                                 <  1:2903081/100‑1 (MQ=255)
                                 aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTAc                                                                >  2:3386428/1‑100 (MQ=255)
                                 aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTAc                                                                >  2:2430798/1‑100 (MQ=255)
                                   tACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGt                                                              >  2:1125999/1‑100 (MQ=255)
                                   tACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGt                                                              >  2:1315119/1‑100 (MQ=255)
                                    aCCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc                                                             <  2:1460290/100‑1 (MQ=255)
                                    aCCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc                                                             >  1:2462971/1‑100 (MQ=255)
                                      cGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTg                                                           <  2:3001996/100‑1 (MQ=255)
                                       gACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc                                                          >  2:3049786/1‑100 (MQ=255)
                                         ccTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCaa                                                        <  2:16807/100‑1 (MQ=255)
                                         ccTGCAGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTg                                                           >  1:1255062/1‑97 (MQ=255)
                                          cTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAt                                                       >  1:3113215/1‑100 (MQ=255)
                                           tGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAtt                                                      >  1:678384/1‑100 (MQ=255)
                                           tGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAtt                                                      <  2:1624180/100‑1 (MQ=255)
                                           tGCCGAACCCGAAAGCGAAGAAGAAGTAATATTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCCCGCCGCTTTTTTTACGTCTGCAAtt                                                      >  1:850996/1‑100 (MQ=255)
                                            tccGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTAAGTCTGCAAttt                                                     >  2:2828800/2‑100 (MQ=255)
                                            gCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAttt                                                     <  2:1723642/100‑1 (MQ=255)
                                             ccGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTa                                                    <  1:60585/100‑1 (MQ=255)
                                              cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc                                                   <  2:2352447/100‑1 (MQ=255)
                                              cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc                                                   <  2:3251725/100‑1 (MQ=255)
                                               gAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAcc                                                  >  1:2392332/1‑100 (MQ=255)
                                               gAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTGTTACGTCTGCAATTTAcc                                                  >  2:2700993/1‑100 (MQ=255)
                                               gAACCCGAAAGCGAAGAAGAAGTAATTGTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAGTGAcc                                                  >  1:624178/1‑100 (MQ=255)
                                                 aCCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCtt                                                >  1:2277376/1‑100 (MQ=255)
                                                   ccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTc                                              >  1:409777/1‑100 (MQ=255)
                                                    cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc                                             >  2:1879188/1‑100 (MQ=255)
                                                     gAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCa                                            >  1:1205717/1‑100 (MQ=255)
                                                     gAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCa                                            >  2:664779/1‑100 (MQ=255)
                                                      aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAg                                           <  1:1733796/100‑1 (MQ=255)
                                                        aGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtc                                         <  2:2634135/100‑1 (MQ=255)
                                                           gaagaagaaGTAATTTTTCGTTTGCGGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttc                                      >  1:1946632/1‑100 (MQ=255)
                                                           gaagaagaaGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttc                                      >  2:405336/1‑100 (MQ=255)
                                                           gaagaagaaGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttc                                      <  2:762021/100‑1 (MQ=255)
                                                            aagaagaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                     <  1:292378/100‑1 (MQ=255)
                                                            aagaagaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                     <  1:3104794/100‑1 (MQ=255)
                                                            aagaagaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                     <  2:3274221/100‑1 (MQ=255)
                                                             agaagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTcttctt                                    >  1:1003082/1‑100 (MQ=255)
                                                                   agTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCGAGTCTTCTTGCTCCa                              >  2:3342700/1‑100 (MQ=255)
                                                                         tttttaattGCCGGATGTTCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCa                        >  2:475539/8‑100 (MQ=38)
                                                                         ttttCGTTTGCCGGAACATCCGTCTATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCa                        <  1:140021/100‑1 (MQ=255)
                                                                         ttttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCa                        <  2:1662449/100‑1 (MQ=255)
                                                                         ttttCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCa                        >  1:3339754/1‑100 (MQ=255)
                                                                            tCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagag                     <  2:3104306/100‑1 (MQ=255)
                                                                              gTTTGCCGGACCATCCGGCAATTAACAAAGCGGCTAACCTCGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCCCCACGTTCagagag                   <  1:2828777/100‑1 (MQ=255)
                                                                              gTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagagag                   >  2:2265200/1‑100 (MQ=255)
                                                                               tttGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAgagaga                  <  1:1008934/100‑1 (MQ=255)
                                                                                ttGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGAc                 <  1:644433/100‑1 (MQ=255)
                                                                                 tGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACg                >  2:1106310/1‑100 (MQ=255)
                                                                                 tGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACg                >  1:2080141/1‑100 (MQ=255)
                                                                                  gCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGt               >  2:2486196/1‑100 (MQ=255)
                                                                                  gCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTGTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGt               >  1:624895/1‑100 (MQ=255)
                                                                                   ccGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGtt              >  2:2325529/1‑100 (MQ=255)
                                                                                     ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg            <  1:2121566/100‑1 (MQ=255)
                                                                                     ggAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg            <  2:2762892/100‑1 (MQ=255)
                                                                                     ggAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg            >  2:1196355/1‑100 (MQ=255)
                                                                                      gAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCTGAGAGACGTTccc           <  2:2299232/100‑3 (MQ=255)
                                                                                       aaCATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCa          <  2:2111181/100‑1 (MQ=255)
                                                                                        aCATCCGTCAATTAAAAAAGCGGCTATCCACGCCGCTTTTTTTACGTCTGCAATTTTCCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg            <  1:883523/97‑1 (MQ=255)
                                                                                        aCATCCGTCAATTAAAAAAGCGGCTAACCACTCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCAt         <  1:1588098/100‑1 (MQ=255)
                                                                                         cATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATa        >  1:99256/1‑100 (MQ=255)
                                                                                             cGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCGCGTTCAGAGAGACGTTCGCATActgc    <  1:3062899/100‑1 (MQ=255)
                                                                                             cGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATActgc    <  2:2656634/100‑1 (MQ=255)
                                                                                              ggCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATActgct   >  1:1492742/1‑100 (MQ=255)
                                                                                              ggCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATActgct   <  2:1532278/100‑1 (MQ=255)
                                                                                               |                                                                                                   
GTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTG  >  NC_000913/129265‑129459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: