Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,169,380 T→C 16.1% intergenic (+50/+32) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,3800TC16.1% 277.4 / 46.3 124intergenic (+50/+32)bhsA/ldtCbiofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base T (53/51);  new base C (12/8);  total (65/59)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-02

Only 100 of 126 total aligned reads displayed.

CTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCA  >  NC_000913/1169281‑1169479
                                                                                                   |                                                                                                   
cTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACAt                                                                                                     >  1:379161/1‑100 (MQ=255)
  gTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGc                                                                                                   >  2:1719128/1‑100 (MQ=255)
    aaCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                 <  2:1320958/100‑1 (MQ=255)
    aaCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                 <  1:2182434/100‑1 (MQ=255)
     aCCGGTCCGAATATCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                 <  1:2532626/99‑1 (MQ=255)
     aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt                                                                                                >  2:2569166/1‑100 (MQ=255)
     aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt                                                                                                >  1:773351/1‑100 (MQ=255)
        ggTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTc                                                                                             >  2:1868720/1‑100 (MQ=255)
        ggTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTc                                                                                             >  2:1209189/1‑100 (MQ=255)
           ccGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTACTGATTTTTTCCCCGCGACATGCCGTGTcgc                                                                                           >  1:1098265/1‑99 (MQ=255)
            cGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                         <  2:2684229/100‑1 (MQ=255)
            cGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                         <  2:1040841/100‑1 (MQ=255)
             gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                        <  2:3139370/100‑1 (MQ=255)
             gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                        <  2:633075/100‑1 (MQ=255)
             gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCggg                                                                                        <  1:1206909/100‑1 (MQ=255)
             ccgatctcTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                        <  2:2933326/93‑1 (MQ=255)
              aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggg                                                                                       >  1:2216964/1‑100 (MQ=255)
              aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggg                                                                                       <  1:2517065/100‑1 (MQ=255)
              aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggg                                                                                       <  2:444242/100‑1 (MQ=255)
              aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCgggg                                                                                       <  2:2162280/100‑1 (MQ=39)
              aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCgggg                                                                                       <  2:1815916/100‑1 (MQ=39)
                  cccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttt                                                                                   >  2:1426388/1‑100 (MQ=255)
                   ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttt                                                                                  >  2:2448925/1‑100 (MQ=255)
                    cTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggagatcgg                                                                                 >  1:2933301/1‑93 (MQ=255)
                      ccATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttttttt                                                                               <  2:2093024/100‑1 (MQ=255)
                      ccATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttttttt                                                                               >  2:1010864/1‑100 (MQ=255)
                       cATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCGGGGaaaaaatca                                                                              <  2:2717080/100‑9 (MQ=37)
                         tGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATc                                                                            >  2:229246/1‑100 (MQ=255)
                           gAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCg                                                                          >  2:118018/1‑100 (MQ=255)
                               agcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCt                                                                      <  2:3205638/100‑1 (MQ=255)
                                gcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCtt                                                                     >  1:596060/1‑100 (MQ=255)
                                gcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCtt                                                                     >  2:19587/1‑100 (MQ=255)
                                 cagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCttt                                                                    >  1:1690245/1‑100 (MQ=255)
                                  agTAATTTATAAATAAGCATTAACCCTCAGTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCt                                                                      <  1:1344541/97‑1 (MQ=255)
                                    tAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTAc                                                                  <  1:2562889/99‑1 (MQ=255)
                                      aTTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGc                                                               <  2:2195280/100‑1 (MQ=255)
                                         tatcAACAAGCATTACCCCTAACTACTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGtt                                                            <  1:1971527/100‑1 (MQ=255)
                                          ataaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGttt                                                           >  2:2209864/1‑100 (MQ=255)
                                           taaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtg                                                          <  2:1953562/100‑1 (MQ=255)
                                             aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCTCGGGGATTTTTTTATCAGGGCTTTACAGCGTTtgtg                                                        >  1:1262459/1‑100 (MQ=255)
                                             aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                        <  1:1010861/100‑1 (MQ=255)
                                             aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                        >  2:922114/1‑100 (MQ=255)
                                             aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                        <  1:2367714/100‑1 (MQ=255)
                                               taaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTggg                                                      <  2:868010/100‑1 (MQ=255)
                                               naaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTTCAGCGTTTGTggg                                                      >  2:784765/2‑100 (MQ=255)
                                                 aGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGGTTTACGGGGGTTGTGGGc                                                     >  1:2787302/1‑99 (MQ=255)
                                                  gCATTAACCCTCATCAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTc                                                   <  1:2660515/100‑1 (MQ=255)
                                                   cATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTTGGc                                                     >  1:1568258/1‑97 (MQ=255)
                                                    aTTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCAc                                                 >  2:2948869/1‑100 (MQ=255)
                                                     ttAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACt                                                >  2:2273937/1‑100 (MQ=255)
                                                       aaCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtg                                              >  2:1276700/1‑100 (MQ=255)
                                                        aCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                             <  1:1209185/100‑1 (MQ=255)
                                                         cccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGCTTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgat                                            >  2:2896045/1‑100 (MQ=255)
                                                          ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATGTTTTTATCCGGGCTTTACAGCGCTTGTGGGCTCACTtgatg                                           >  1:275970/1‑100 (MQ=255)
                                                                tAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcg                                     >  1:2520502/1‑100 (MQ=255)
                                                                tAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcg                                     <  2:596063/100‑1 (MQ=255)
                                                                 ttccgatcttACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                    <  2:1841067/91‑1 (MQ=255)
                                                                 cgatcttGCTACTGCTGATTTCTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                    <  1:2155163/94‑1 (MQ=255)
                                                                 aaTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                    <  2:621386/100‑1 (MQ=255)
                                                                  aTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGTGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                    <  1:1287898/99‑1 (MQ=255)
                                                                    gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                 >  1:649726/1‑100 (MQ=255)
                                                                    gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                 >  2:2097723/1‑100 (MQ=255)
                                                                     ccTGCTACTGCTGATTTTTTCCCCGCGACGTGCCGTGTCCCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACAt                                <  1:2957462/100‑1 (MQ=255)
                                                                      cTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTTTTTTTTCTTTACAGCGTTTGTGGGCTCACTTTATGTCTGCGTACAt                                >  1:1601688/1‑99 (MQ=255)
                                                                       tGCTACTGCTGATTTCTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGAGATcg                              >  2:2155178/1‑99 (MQ=255)
                                                                         cTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTGTCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGTGGatgtccgc                            <  1:137811/100‑8 (MQ=255)
                                                                          tACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGAGATcggaa                           >  1:1841056/1‑96 (MQ=255)
                                                                             tgctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGc                        >  2:2819675/1‑100 (MQ=255)
                                                                             tgctgATTTTCTCCGCGCGACATGACGTGTAGCGGGCGTTTTTTTACCCGGGCTTTACAGCGTTTGTGGGCTCACTTTATGACGGCGGACAGCCACCGGc                        <  1:3170685/100‑1 (MQ=255)
                                                                              gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCa                       <  2:2480286/100‑1 (MQ=255)
                                                                              gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCa                       <  1:2615253/100‑1 (MQ=255)
                                                                              gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCa                       <  2:3242831/100‑1 (MQ=255)
                                                                              gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGCGGGCTCACTTGATGACGGCGGACATCCACCGGCa                       >  2:1467145/1‑100 (MQ=255)
                                                                               ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCAt                      >  1:2749979/1‑100 (MQ=255)
                                                                               ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCAt                      >  1:500756/1‑100 (MQ=255)
                                                                                 gATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATcc                    >  1:1720707/1‑100 (MQ=255)
                                                                                  aaaaaatCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATccc                   >  1:3005285/7‑100 (MQ=37)
                                                                                   ttttttCCCCGTGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaag                  <  1:2344380/100‑4 (MQ=37)
                                                                                   ttttttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTTTTTTTTTTTTACAGCGTTTGGGGGCTCACTTGCTGACGTCGTACGTCCACCGGCATCCCg                  >  1:2214379/1‑100 (MQ=255)
                                                                                   ttttttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaag                  <  2:1598248/100‑4 (MQ=37)
                                                                                   ttgtttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCg                  <  1:2637148/97‑1 (MQ=255)
                                                                                     ttttCCCCGCGACATGCCGTGTCGCGGGGATTGTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaagtg                <  2:3144669/100‑6 (MQ=25)
                                                                                       ttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACTGGCATCCCGGAAc              <  2:2252494/100‑1 (MQ=255)
                                                                                       ttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGaa               <  2:2828557/99‑1 (MQ=255)
                                                                                        tCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACg             >  2:3052751/1‑100 (MQ=39)
                                                                                         ccccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg            >  2:1883280/1‑100 (MQ=255)
                                                                                         ccccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg            >  2:2527919/1‑100 (MQ=255)
                                                                                         ccccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGCACgg            >  1:30465/1‑100 (MQ=38)
                                                                                         ccccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg            >  2:1216033/1‑100 (MQ=39)
                                                                                          cccGCGAGCTGCCGTGTCGCCGGGACGTTTTTATCCGCGCTATACAGCGGTTGTGGGCGCACTTGATCACGGTGGACACACAAAGGCATCCCGGAACgg            >  1:919434/1‑99 (MQ=255)
                                                                                          cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg            >  1:2433414/1‑99 (MQ=39)
                                                                                          cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa           >  2:419977/1‑100 (MQ=255)
                                                                                          cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa           >  2:724886/1‑100 (MQ=255)
                                                                                          cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa           >  2:419945/1‑100 (MQ=255)
                                                                                          cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCCGAACgg            >  1:1991279/1‑99 (MQ=39)
                                                                                           ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAc          <  1:3189230/100‑1 (MQ=255)
                                                                                             gcgACATGCCGTGTCGCGTGGATTTTTTTGTCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAca         <  1:2017649/99‑1 (MQ=255)
                                                                                               gACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc      <  2:2729340/100‑1 (MQ=255)
                                                                                                aCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc      <  1:2620762/99‑1 (MQ=255)
                                                                                                   tGCCGTGTCGCGGGGATTTTTTTATCTGGGCTTTACAGCGTTTGTGGGCTTACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATca  >  1:2386894/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCA  >  NC_000913/1169281‑1169479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: