Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,169,382 C→G 16.0% intergenic (+52/+30) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,3820CG16.0% 334.0 / 57.5 126intergenic (+52/+30)bhsA/ldtCbiofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base C (55/50);  new base G (12/8);  total (67/59)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01

Only 100 of 128 total aligned reads displayed.

GTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCAC  >  NC_000913/1169283‑1169480
                                                                                                   |                                                                                                  
gTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGc                                                                                                    <  1:3080195/100‑1 (MQ=255)
gTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGc                                                                                                    >  2:1719128/1‑100 (MQ=255)
  aaCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                  <  2:1320958/100‑1 (MQ=255)
  aaCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                  <  1:2532884/100‑1 (MQ=255)
   aCCGGTCCGAATATCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                  <  1:2532626/99‑1 (MQ=255)
   aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAATCATTAACCCGCATTAATGCCTGCTACGGCGGATTTTTTCCCCTCGATATGCGgt                                                                                                 >  1:2828534/1‑100 (MQ=255)
   aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt                                                                                                 >  1:773351/1‑100 (MQ=255)
   aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt                                                                                                 >  2:2569166/1‑100 (MQ=255)
      ggTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTc                                                                                              >  2:1868720/1‑100 (MQ=255)
         ccGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTACTGATTTTTTCCCCGCGACATGCCGTGTcgc                                                                                            >  1:1098265/1‑99 (MQ=255)
          cGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                          <  2:1040841/100‑1 (MQ=255)
          cGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                          <  2:2684229/100‑1 (MQ=255)
           gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                         <  2:3139370/100‑1 (MQ=255)
           gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                         <  2:633075/100‑1 (MQ=255)
           gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCggg                                                                                         <  1:1206909/100‑1 (MQ=255)
           gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCggg                                                                                         <  2:1805371/100‑1 (MQ=255)
            aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggg                                                                                        >  1:2216964/1‑100 (MQ=255)
            aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggg                                                                                        <  2:444242/100‑1 (MQ=255)
            aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCgggg                                                                                        <  2:2162280/100‑1 (MQ=39)
                 ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttt                                                                                   >  2:2448925/1‑100 (MQ=255)
                    ccATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttttttt                                                                                <  2:2093024/100‑1 (MQ=255)
                     cATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCGGGGaaaaaatca                                                                               <  2:2717080/100‑9 (MQ=37)
                         gAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCg                                                                           >  2:118018/1‑100 (MQ=255)
                          aaCAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCgg                                                                          >  2:3022247/1‑100 (MQ=255)
                           acagacgTAATTTATAAATAAGCAGTAACTCTCATTACTGCCTGCTACTGCTGATGTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATcc                                                                            <  1:24416/91‑1 (MQ=255)
                            cagcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGc                                                                        <  2:2824356/100‑1 (MQ=255)
                             agcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCt                                                                       <  2:3205638/100‑1 (MQ=255)
                              gcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCtt                                                                      >  2:19587/1‑100 (MQ=255)
                               cagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCttt                                                                     >  1:1690245/1‑100 (MQ=255)
                                agTAATTTATAAATAAGCATTAACCCTCAGTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCt                                                                       <  1:1344541/97‑1 (MQ=255)
                                  tAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTAc                                                                   <  1:2562889/99‑1 (MQ=255)
                                    aTTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGc                                                                <  2:2195280/100‑1 (MQ=255)
                                      ttATACATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCg                                                               >  1:2650458/1‑99 (MQ=255)
                                       tatcAACAAGCATTACCCCTAACTACTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGtt                                                             <  1:1971527/100‑1 (MQ=255)
                                         taaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtg                                                           <  2:1953562/100‑1 (MQ=255)
                                          aaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgt                                                          >  1:2275528/1‑100 (MQ=255)
                                           aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCTCGGGGATTTTTTTATCAGGGCTTTACAGCGTTtgtg                                                         >  1:1262459/1‑100 (MQ=255)
                                           aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                         <  1:1010861/100‑1 (MQ=255)
                                           aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                         >  2:922114/1‑100 (MQ=255)
                                            ataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTgg                                                        <  2:2216979/100‑1 (MQ=255)
                                             taaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTggg                                                       <  2:868010/100‑1 (MQ=255)
                                             naaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTTCAGCGTTTGTggg                                                       >  2:784765/2‑100 (MQ=255)
                                               aGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGGTTTACGGGGGTTGTGGGc                                                      >  1:2787302/1‑99 (MQ=255)
                                               aGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCt                                                     <  1:966101/100‑1 (MQ=255)
                                                gCATTAACCCTCATCAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTc                                                    <  1:2660515/100‑1 (MQ=255)
                                                 cATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTTGGc                                                      >  1:1568258/1‑97 (MQ=255)
                                                  aTTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCAc                                                  >  2:2948869/1‑100 (MQ=255)
                                                   ttAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACt                                                 >  2:2273937/1‑100 (MQ=255)
                                                     aaCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtg                                               >  2:1276700/1‑100 (MQ=255)
                                                      aCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                              <  1:1209185/100‑1 (MQ=255)
                                                       cccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGCTTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgat                                             >  2:2896045/1‑100 (MQ=255)
                                                        ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgatg                                            >  2:2639027/1‑100 (MQ=255)
                                                        ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATGTTTTTATCCGGGCTTTACAGCGCTTGTGGGCTCACTtgatg                                            >  1:275970/1‑100 (MQ=255)
                                                              tAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcg                                      <  2:596063/100‑1 (MQ=255)
                                                              tAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcg                                      >  1:2520502/1‑100 (MQ=255)
                                                               ttccgatcttACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                     <  2:1841067/91‑1 (MQ=255)
                                                               cgatcttGCTACTGCTGATTTCTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                     <  1:2155163/94‑1 (MQ=255)
                                                               aaTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                     <  2:621386/100‑1 (MQ=255)
                                                                aTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGTGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                     <  1:1287898/99‑1 (MQ=255)
                                                                  gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                  >  2:1188769/1‑100 (MQ=255)
                                                                  gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                  >  1:649726/1‑100 (MQ=255)
                                                                  gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                  >  2:2097723/1‑100 (MQ=255)
                                                                  gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                  >  2:3235226/1‑100 (MQ=255)
                                                                   ccTGCTACTGCTGATTTTTTCCCCGCGACGTGCCGTGTCCCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACAt                                 <  1:2957462/100‑1 (MQ=255)
                                                                    cTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTTTTTTTTCTTTACAGCGTTTGTGGGCTCACTTTATGTCTGCGTACAt                                 >  1:1601688/1‑99 (MQ=255)
                                                                     tGCTACTGCTGATTTCTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGAGATcg                               >  2:2155178/1‑99 (MQ=255)
                                                                       cTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTGTCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGTGGatgtccgc                             <  1:137811/100‑8 (MQ=255)
                                                                        tACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGAGATcggaa                            >  1:1841056/1‑96 (MQ=255)
                                                                         aCTGCTGATTTTTTCCCCACTGCATGCATTGTCGCGAACATTTTTTTATTCAGGCTTTACAGCGTTTGAGTTCTCACTTGATGACGGCGGACa                                  <  1:2131179/93‑1 (MQ=255)
                                                                           tgctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGc                         >  2:2819675/1‑100 (MQ=255)
                                                                            gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCa                        <  2:2480286/100‑1 (MQ=255)
                                                                            gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGCGGGCTCACTTGATGACGGCGGACATCCACCGGCa                        >  2:1467145/1‑100 (MQ=255)
                                                                             ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCAt                       >  1:500756/1‑100 (MQ=255)
                                                                                aaaaaatCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATccc                    >  1:3005285/7‑100 (MQ=37)
                                                                                 ttttttCCCCGTGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaag                   <  1:2344380/100‑4 (MQ=37)
                                                                                 ttttttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTTTTTTTTTTTTACAGCGTTTGGGGGCTCACTTGCTGACGTCGTACGTCCACCGGCATCCCg                   >  1:2214379/1‑100 (MQ=255)
                                                                                 ttttttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaag                   <  2:1598248/100‑4 (MQ=37)
                                                                                 ttgtttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCg                   <  1:2637148/97‑1 (MQ=255)
                                                                                   ttttCCCCGCGACATGCCGTGTCGCGGGGATTGTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaagtg                 <  2:3144669/100‑6 (MQ=25)
                                                                                     ttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACTGGCATCCCGGAAc               <  2:2252494/100‑1 (MQ=255)
                                                                                     ttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGaa                <  2:2828557/99‑1 (MQ=255)
                                                                                       ccccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:1371727/1‑100 (MQ=255)
                                                                                       ccccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:1883280/1‑100 (MQ=255)
                                                                                       ccccGCGACATGCCGTGTCGCGGGGATTTTTTTACCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCCTCCAGGAACgg             <  1:1781594/100‑1 (MQ=255)
                                                                                       ccccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGCACgg             >  1:30465/1‑100 (MQ=38)
                                                                                       ccccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:245540/1‑100 (MQ=39)
                                                                                       ccccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:1216033/1‑100 (MQ=39)
                                                                                        cccGCGAGCTGCCGTGTCGCCGGGACGTTTTTATCCGCGCTATACAGCGGTTGTGGGCGCACTTGATCACGGTGGACACACAAAGGCATCCCGGAACgg             >  1:919434/1‑99 (MQ=255)
                                                                                        cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  1:2433414/1‑99 (MQ=39)
                                                                                        cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  2:419945/1‑100 (MQ=255)
                                                                                        cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  1:506440/1‑100 (MQ=255)
                                                                                        cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  2:724886/1‑100 (MQ=255)
                                                                                        cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  2:419977/1‑100 (MQ=255)
                                                                                         ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAc           <  1:3189230/100‑1 (MQ=255)
                                                                                           gcgACATGCCGTGTCGCGTGGATTTTTTTGTCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAca          <  1:2017649/99‑1 (MQ=255)
                                                                                              aCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc       <  1:2620762/99‑1 (MQ=255)
                                                                                                 tGCCGTGTCGCGGGGATTTTTTTATCTGGGCTTTACAGCGTTTGTGGGCTTACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATca   >  1:2386894/1‑100 (MQ=255)
                                                                                                  gCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGAGGACATCCACCGGCATCCCGGAACGGAcatcca      >  1:1868990/1‑96 (MQ=255)
                                                                                                  gCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATcac  <  2:2269645/100‑1 (MQ=255)
                                                                                                  gCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATcac  >  2:2964222/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCAC  >  NC_000913/1169283‑1169480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: