Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,169,384 G→A 16.9% intergenic (+54/+28) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,3840GA16.9% 273.9 / 43.2 119intergenic (+54/+28)bhsA/ldtCbiofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base G (52/46);  new base A (12/8);  total (64/55)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

Only 100 of 126 total aligned reads displayed.

AACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCAC  >  NC_000913/1169285‑1169480
                                                                                                   |                                                                                                
aaCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                  <  2:1320958/100‑1 (MQ=255)
aaCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                  <  1:2532884/100‑1 (MQ=255)
aaCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                  <  1:2182434/100‑1 (MQ=255)
 aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAATCATTAACCCGCATTAATGCCTGCTACGGCGGATTTTTTCCCCTCGATATGCGgt                                                                                                 >  1:2828534/1‑100 (MQ=255)
 aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt                                                                                                 >  1:773351/1‑100 (MQ=255)
 aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt                                                                                                 >  2:2569166/1‑100 (MQ=255)
    ggTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTc                                                                                              >  2:1868720/1‑100 (MQ=255)
       ccGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTACTGATTTTTTCCCCGCGACATGCCGTGTcgc                                                                                            >  1:1098265/1‑99 (MQ=255)
        cGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgg                                                                                          <  2:1040841/100‑1 (MQ=255)
         gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                         <  2:3139370/100‑1 (MQ=255)
         gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                         <  2:633075/100‑1 (MQ=255)
         gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCggg                                                                                         <  1:1206909/100‑1 (MQ=255)
         gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCggg                                                                                         <  2:1805371/100‑1 (MQ=255)
         ccgatctcTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCggg                                                                                         <  2:2933326/93‑1 (MQ=255)
          aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggg                                                                                        <  2:444242/100‑1 (MQ=255)
          aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggg                                                                                        <  1:2517065/100‑1 (MQ=255)
          aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCgggg                                                                                        <  2:1815916/100‑1 (MQ=39)
            tACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCGGGGaa                                                                                      <  2:214114/100‑2 (MQ=38)
               ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttt                                                                                   >  2:2448925/1‑100 (MQ=255)
                cTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgggagatcgg                                                                                  >  1:2933301/1‑93 (MQ=255)
                  ccATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttttttt                                                                                <  2:2093024/100‑1 (MQ=255)
                   cATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCGGGGaaaaaatca                                                                               <  2:2717080/100‑9 (MQ=37)
                     tGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATc                                                                             >  2:229246/1‑100 (MQ=255)
                       gAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCg                                                                           >  2:118018/1‑100 (MQ=255)
                         acagacgTAATTTATAAATAAGCAGTAACTCTCATTACTGCCTGCTACTGCTGATGTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATcc                                                                            <  1:24416/91‑1 (MQ=255)
                          cagcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGc                                                                        <  2:2824356/100‑1 (MQ=255)
                           agcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCt                                                                       <  2:3205638/100‑1 (MQ=255)
                             cagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCttt                                                                     >  1:1690245/1‑100 (MQ=255)
                              agTAATTTATAAATAAGCATTAACCCTCAGTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCt                                                                       <  1:1344541/97‑1 (MQ=255)
                                tAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTAc                                                                   <  1:2562889/99‑1 (MQ=255)
                                  aTTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGc                                                                <  2:2195280/100‑1 (MQ=255)
                                    ttATACATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCg                                                               >  1:2650458/1‑99 (MQ=255)
                                     tatcAACAAGCATTACCCCTAACTACTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGtt                                                             <  1:1971527/100‑1 (MQ=255)
                                      ataaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGttt                                                            >  2:2209864/1‑100 (MQ=255)
                                       taaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtg                                                           <  2:1953562/100‑1 (MQ=255)
                                        aaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgt                                                          >  1:2275528/1‑100 (MQ=255)
                                         aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCTCGGGGATTTTTTTATCAGGGCTTTACAGCGTTtgtg                                                         >  1:1262459/1‑100 (MQ=255)
                                         aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                         <  1:1010861/100‑1 (MQ=255)
                                         aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                         >  2:922114/1‑100 (MQ=255)
                                         aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                         <  1:2367714/100‑1 (MQ=255)
                                          ataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTgg                                                        <  2:2216979/100‑1 (MQ=255)
                                           taaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTggg                                                       <  2:868010/100‑1 (MQ=255)
                                           naaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTTCAGCGTTTGTggg                                                       >  2:784765/2‑100 (MQ=255)
                                             aGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCt                                                     <  1:966101/100‑1 (MQ=255)
                                              gCATTAACCCTCATCAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTc                                                    <  1:2660515/100‑1 (MQ=255)
                                               cATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTTGGc                                                      >  1:1568258/1‑97 (MQ=255)
                                                aTTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCAc                                                  >  2:2948869/1‑100 (MQ=255)
                                                 ttAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACt                                                 >  2:2273937/1‑100 (MQ=255)
                                                   aaCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtg                                               >  2:1276700/1‑100 (MQ=255)
                                                    aCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                              <  1:1209185/100‑1 (MQ=255)
                                                     cccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGCTTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgat                                             >  2:2896045/1‑100 (MQ=255)
                                                      ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgatg                                            >  2:2639027/1‑100 (MQ=255)
                                                      ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATGTTTTTATCCGGGCTTTACAGCGCTTGTGGGCTCACTtgatg                                            >  1:275970/1‑100 (MQ=255)
                                                            tAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcg                                      <  2:596063/100‑1 (MQ=255)
                                                            tAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcg                                      >  1:2520502/1‑100 (MQ=255)
                                                             cgatcttGCTACTGCTGATTTCTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                     <  1:2155163/94‑1 (MQ=255)
                                                             aaTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                     <  2:621386/100‑1 (MQ=255)
                                                              aTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGTGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                     <  1:1287898/99‑1 (MQ=255)
                                                                gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                  >  2:1188769/1‑100 (MQ=255)
                                                                gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                  >  1:649726/1‑100 (MQ=255)
                                                                gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                  >  2:2097723/1‑100 (MQ=255)
                                                                 ccTGCTACTGCTGATTTTTTCCCCGCGACGTGCCGTGTCCCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACAt                                 <  1:2957462/100‑1 (MQ=255)
                                                                  cTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTTTTTTTTCTTTACAGCGTTTGTGGGCTCACTTTATGTCTGCGTACAt                                 >  1:1601688/1‑99 (MQ=255)
                                                                   tGCTACTGCTGATTTCTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGAGATcg                               >  2:2155178/1‑99 (MQ=255)
                                                                     cTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTGTCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGTGGatgtccgc                             <  1:137811/100‑8 (MQ=255)
                                                                      tACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGAGATcggaa                            >  1:1841056/1‑96 (MQ=255)
                                                                       aCTGCTGATTTTTTCCCCACTGCATGCATTGTCGCGAACATTTTTTTATTCAGGCTTTACAGCGTTTGAGTTCTCACTTGATGACGGCGGACa                                  <  1:2131179/93‑1 (MQ=255)
                                                                         tgctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGc                         >  2:2819675/1‑100 (MQ=255)
                                                                         tgctgATTTTCTCCGCGCGACATGACGTGTAGCGGGCGTTTTTTTACCCGGGCTTTACAGCGTTTGTGGGCTCACTTTATGACGGCGGACAGCCACCGGc                         <  1:3170685/100‑1 (MQ=255)
                                                                          gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCa                        <  2:3242831/100‑1 (MQ=255)
                                                                          gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCa                        <  1:2615253/100‑1 (MQ=255)
                                                                          gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGCGGGCTCACTTGATGACGGCGGACATCCACCGGCa                        >  2:1467145/1‑100 (MQ=255)
                                                                           ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCAt                       >  1:2749979/1‑100 (MQ=255)
                                                                           ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCAt                       >  1:500756/1‑100 (MQ=255)
                                                                             gATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATcc                     >  1:1720707/1‑100 (MQ=255)
                                                                              aaaaaatCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATccc                    >  1:3005285/7‑100 (MQ=37)
                                                                               ttttttCCCCGTGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaag                   <  1:2344380/100‑4 (MQ=37)
                                                                               ttttttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTTTTTTTTTTTTACAGCGTTTGGGGGCTCACTTGCTGACGTCGTACGTCCACCGGCATCCCg                   >  1:2214379/1‑100 (MQ=255)
                                                                               ttttttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaag                   <  2:1598248/100‑4 (MQ=37)
                                                                                 ttttCCCCGCGACATGCCGTGTCGCGGGGATTGTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATcaagtg                 <  2:3144669/100‑6 (MQ=25)
                                                                                   ttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACTGGCATCCCGGAAc               <  2:2252494/100‑1 (MQ=255)
                                                                                    tCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACg              >  2:3052751/1‑100 (MQ=39)
                                                                                     ccccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:2527919/1‑100 (MQ=255)
                                                                                     ccccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:1883280/1‑100 (MQ=255)
                                                                                     ccccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:1371727/1‑100 (MQ=255)
                                                                                     ccccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:245540/1‑100 (MQ=39)
                                                                                     ccccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg             >  2:1216033/1‑100 (MQ=39)
                                                                                      cccGCGAGCTGCCGTGTCGCCGGGACGTTTTTATCCGCGCTATACAGCGGTTGTGGGCGCACTTGATCACGGTGGACACACAAAGGCATCCCGGAACgg             >  1:919434/1‑99 (MQ=255)
                                                                                      cccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  2:1898440/1‑100 (MQ=255)
                                                                                      cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  1:506440/1‑100 (MQ=255)
                                                                                      cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  2:724886/1‑100 (MQ=255)
                                                                                      cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  2:419945/1‑100 (MQ=255)
                                                                                      cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa            >  2:419977/1‑100 (MQ=255)
                                                                                      cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCCGAACgg             >  1:1991279/1‑99 (MQ=39)
                                                                                       ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAc           <  1:3189230/100‑1 (MQ=255)
                                                                                           gACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc       <  2:2729340/100‑1 (MQ=255)
                                                                                            aCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc       <  1:2620762/99‑1 (MQ=255)
                                                                                               tGCCGTGTCGCGGGGATTTTTTTATCTGGGCTTTACAGCGTTTGTGGGCTTACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATca   >  1:2386894/1‑100 (MQ=255)
                                                                                                gCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGAGGACATCCACCGGCATCCCGGAACGGAcatcca      >  1:1868990/1‑96 (MQ=255)
                                                                                                gCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATcac  >  2:2964222/1‑100 (MQ=255)
                                                                                                   |                                                                                                
AACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCAC  >  NC_000913/1169285‑1169480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: