Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,172,553 A→G 30.9% I471T (ATC→ACC)  mfd ← transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,172,5530AG30.9% 147.0 / 87.1 116I471T (ATC→ACC) mfdtranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base A (39/41);  new base G (20/16);  total (59/57)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

ATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGC  >  NC_000913/1172454‑1172651
                                                                                                   |                                                                                                  
aTGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACAACCGGCTGACGAATATGCAGTTCCGCAGGGTTACGGa                                                                                                    >  1:3071226/1‑100 (MQ=255)
    cACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGGCCAATATGCAGTTCCGAAAGGTTACGGAACAg                                                                                                >  1:43389/1‑100 (MQ=255)
     aCCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAgt                                                                                               <  1:584180/100‑1 (MQ=255)
     aCCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAgt                                                                                               <  1:2812479/100‑1 (MQ=255)
      cccGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAgtg                                                                                              <  2:1671197/100‑1 (MQ=255)
       ccGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAg                                                                                                <  1:2558788/97‑1 (MQ=255)
        ngCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTa                                                                                            >  2:1530741/2‑100 (MQ=255)
          cTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATc                                                                                          <  1:2175168/100‑1 (MQ=255)
           ttCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCg                                                                                         <  2:3121507/100‑1 (MQ=255)
             ccAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGGTACGGATCAGTGTATCggg                                                                                       >  1:3027421/1‑100 (MQ=255)
               aGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGt                                                                                     >  2:409692/1‑100 (MQ=255)
                gCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGtt                                                                                    <  2:2426995/100‑1 (MQ=255)
                 cGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTg                                                                                   >  1:2303405/1‑100 (MQ=255)
                    ggTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGAAGAGTGTATCGGGGTTGATg                                                                                >  1:1643212/1‑100 (MQ=255)
                      tCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGt                                                                              >  2:1799227/1‑100 (MQ=255)
                      tCATTCCGGCATAACGACCGACGCCGTCCTCCAGATTGCCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATgg                                                                               >  1:282719/1‑99 (MQ=255)
                        aTTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTgc                                                                            >  2:3006629/1‑100 (MQ=255)
                          tCCGCCATAACGACCGACGCCGTGCTCCAGATGGCCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTgcg                                                                           >  1:3229154/1‑99 (MQ=255)
                           ccGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTgcggc                                                                         <  1:1962344/100‑1 (MQ=255)
                            cGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTgcggcg                                                                        <  2:1776345/100‑1 (MQ=255)
                              gCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCgag                                                                      <  2:3175613/100‑1 (MQ=255)
                              gCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCgag                                                                      <  2:52515/100‑1 (MQ=255)
                               cATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCgaga                                                                     <  1:1808267/100‑1 (MQ=255)
                                   aCGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATcc                                                                 >  2:3028543/1‑100 (MQ=255)
                                   aCGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATcc                                                                 <  1:2656314/100‑1 (MQ=255)
                                   aCGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATcc                                                                 >  2:621366/1‑100 (MQ=255)
                                   aCGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACCGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATcc                                                                 <  1:1801818/100‑1 (MQ=255)
                                    cgaccgacGCCGTGCTCCAGATGGACCACCGGCTGCCCAATATGCAGTTCCGCCAGGCTATGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCt                                                                >  1:2383977/1‑100 (MQ=255)
                                     gaccgacGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTg                                                               <  2:2847383/100‑1 (MQ=255)
                                       ccgacGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTgac                                                             >  1:2198312/1‑100 (MQ=255)
                                       ccgacGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTgac                                                             >  2:1883113/1‑100 (MQ=255)
                                             ccGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTgacgacgac                                                       <  1:2103964/100‑1 (MQ=255)
                                                 gCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGc                                                   <  1:1347336/100‑1 (MQ=255)
                                                 gCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGc                                                   <  1:378728/100‑1 (MQ=255)
                                                  cTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCa                                                  >  2:2739454/1‑100 (MQ=255)
                                                  cTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCa                                                  >  2:1666349/1‑100 (MQ=255)
                                                  cTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCa                                                  >  2:1971763/1‑100 (MQ=255)
                                                   tCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCaa                                                 >  1:2022775/1‑100 (MQ=255)
                                                   tCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCaa                                                 >  2:2860070/1‑100 (MQ=255)
                                                   tCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCa                                                  >  1:1849544/1‑99 (MQ=255)
                                                    ccAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAAc                                                <  1:1600831/100‑1 (MQ=255)
                                                    ccAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAAc                                                >  2:641955/1‑100 (MQ=255)
                                                     cAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAAcg                                               >  1:3177702/1‑100 (MQ=255)
                                                       gATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAAcgcg                                             >  1:1105854/1‑100 (MQ=255)
                                                        aTGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGt                                            <  2:1134292/100‑1 (MQ=255)
                                                          ggACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTc                                          >  1:245710/1‑100 (MQ=255)
                                                           gACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCa                                         >  1:57970/1‑100 (MQ=255)
                                                            accaccGGCTGACCACTATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCAc                                        <  2:3177729/100‑1 (MQ=255)
                                                            accaccGGCTGACCAAGATGCAGTGCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCAc                                        <  1:3130345/100‑1 (MQ=255)
                                                             ccaccGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGCCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCAcc                                       >  1:3052634/1‑100 (MQ=255)
                                                               accGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGa                                     >  1:970624/1‑100 (MQ=255)
                                                               accGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGa                                     <  2:273572/100‑1 (MQ=255)
                                                               accGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGa                                     <  1:2066257/100‑1 (MQ=255)
                                                               accGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGa                                     >  1:516660/1‑100 (MQ=255)
                                                               aacGGCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGa                                     >  2:1329960/3‑100 (MQ=255)
                                                                ccGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAg                                    <  1:614367/100‑1 (MQ=255)
                                                                ccGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAg                                    <  1:1181657/100‑1 (MQ=255)
                                                                  ggCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCa                                  <  2:1105857/100‑1 (MQ=255)
                                                                   gCTGACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAg                                 >  2:2656377/1‑100 (MQ=255)
                                                                     tGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGAt                               <  1:1149343/100‑1 (MQ=255)
                                                                     tGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGAt                               <  2:3387741/100‑1 (MQ=255)
                                                                     tGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGCTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGAt                               >  2:617701/1‑100 (MQ=255)
                                                                     tGACCAATATGCAGTGCCGCAAGGTTACGGATCAGTGGATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGAGCAACGCGTCCACCGAGCAGa                                <  1:1455423/99‑1 (MQ=255)
                                                                      gACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATc                              <  2:47726/100‑1 (MQ=255)
                                                                      gACCAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATc                              <  1:2749936/100‑1 (MQ=255)
                                                                      gACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATc                              <  1:978787/100‑1 (MQ=255)
                                                                       aCCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCg                             <  1:3001823/100‑1 (MQ=255)
                                                                        ccAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGc                            >  2:2560875/1‑100 (MQ=255)
                                                                         cAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCACCGCGTTCACCGAGCAGAACGc                            >  1:3307033/1‑99 (MQ=255)
                                                                         cAATATGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCt                           <  1:409690/100‑1 (MQ=255)
                                                                           atatGCAGTTCCGCACGGTTGCGGGTCAGTGTATCGTGGTTGGTGGAGCGGCGAGTATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCg                             <  1:1147692/96‑1 (MQ=255)
                                                                           atatGCAGTCCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCttt                         <  2:1434918/100‑1 (MQ=255)
                                                                            tatGCAGTTCCGCAATGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTc                        <  1:291015/100‑1 (MQ=255)
                                                                            tatGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTc                        <  1:2566638/100‑1 (MQ=255)
                                                                            tatGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTc                        <  1:977302/100‑1 (MQ=255)
                                                                            tatGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTc                        <  2:551446/100‑1 (MQ=255)
                                                                             atGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCg                       <  2:2878938/100‑1 (MQ=255)
                                                                              tGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGc                      >  1:1179635/1‑100 (MQ=255)
                                                                              tGCAGTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGc                      >  1:683964/1‑100 (MQ=255)
                                                                              tGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGc                      >  1:1713007/1‑100 (MQ=255)
                                                                              tGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGc                      <  1:2680346/100‑1 (MQ=255)
                                                                              tGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGc                      >  2:2542329/1‑100 (MQ=255)
                                                                              tGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGc                      <  2:2006860/100‑1 (MQ=255)
                                                                                 aGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCaaa                   <  2:445197/100‑1 (MQ=255)
                                                                                  gTTCCGCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAAt                  >  1:1550047/1‑100 (MQ=255)
                                                                                     ccGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAg               >  2:2753715/1‑100 (MQ=255)
                                                                                     ccGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAg               >  2:394483/1‑100 (MQ=255)
                                                                                      cGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAgc              >  2:2411328/1‑100 (MQ=255)
                                                                                       gCAAGGTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAgcg             <  2:2198327/100‑1 (MQ=255)
                                                                                       gCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAgcg             >  1:1230936/1‑100 (MQ=255)
                                                                                       gCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCCTTCGCAAATCAgcg             >  2:3236636/1‑100 (MQ=255)
                                                                                          aGGCTACGGATCAGTGTAGCGGGGTTAATGGTGCGGAGAGAATCCTGACGACGACGGGCAAAGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCa          >  1:3360676/1‑100 (MQ=255)
                                                                                           ggTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAg         <  1:1394257/100‑1 (MQ=255)
                                                                                           ggTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAg         >  2:1600120/1‑100 (MQ=255)
                                                                                           ggTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAg         >  2:2309961/1‑100 (MQ=255)
                                                                                            gTTACGGGTCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGa        >  1:2342792/1‑100 (MQ=255)
                                                                                             ttACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGAt       <  1:2535640/100‑1 (MQ=255)
                                                                                                cGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTAc    >  2:427102/1‑100 (MQ=255)
                                                                                                cGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTAc    <  1:2445415/100‑1 (MQ=255)
                                                                                                  gATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGc  >  2:1217482/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
ATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGC  >  NC_000913/1172454‑1172651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: